Gene

KWMTBOMO07184  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA010392

Annotation

PREDICTED:_uncharacterized_protein_LOC101743036_[Bombyx_mori]

Location

Bomo_Chr12(-):7246373-7248161      View in EpiBrowser

Full name

Juvenile hormone acid O-methyltransferase      

Alternative Name

Juvenile hormone acid methyltransferase

Location in the cell

Cytoplasmic   Reliability : 2.201 Nuclear   Reliability : 2.153

CDS

ATGAACGACCATCTAGACTTTTACCATGACTGCAACGATGTTACCAGAATAGATGCTATTGAATCTCTAACGGAATACGGATCGAAACTTAAATTCAAACCAAAAGCAAAAGTTATTGAAGTAGGATGCGCTGACGGCAGCGTTTCTAACATCCTTTTCCAACATTTGCCCAAGGACATAGAGCTTTTGCTATCTTGCGATAAAAATGAGAAAGCGGTACAGTTTGCCAAGGAGCACTACAAAAATAACAAAACAGCTTATAGGGTATTAGATATAGAAGGCGACCTGCCGGAGGATTTAAAAGGGAAATTCGATAATTTCATCTCATTGATGACGTTCCATTGGGTCCCTCAACAGGAAAAAGCATTTCGAAACGTCTACGATCTTCTGGCTGAGGACGGAGAATGTTTTTTGACCTTAAAATCGTATTCACACATTTACCATATGTTTGTATTACAATCAAAATCAAGAAAATGGGGTCCGTTCATGAAGAATATGAAAAACTTTCTGTCGCCGTACTACGATTTGTCGATGCCGGATCAACACATAGCAAAAATATTAAAGAACGTCGGTTTCAAGAATATTGACGTAAGATCTAAACAGAAAATGTACACATTTAAGAACTTGGAGAAGTTTAGGGGACTCATGATCGGAGTGAATCCATTTAAAGTTCCTGAGAATGAATTTGAAAATTACATTGACGACCTCATGGAAACAGCACGCACGCTACGTATTATTGATGAGGAAAACGGCACGTTGTCTTTCCTGTTTAATATGAACATTGTTCATTGTACTAAATAA

Protein

MNDHLDFYHDCNDVTRIDAIESLTEYGSKLKFKPKAKVIEVGCADGSVSNILFQHLPKDIELLLSCDKNEKAVQFAKEHYKNNKTAYRVLDIEGDLPEDLKGKFDNFISLMTFHWVPQQEKAFRNVYDLLAEDGECFLTLKSYSHIYHMFVLQSKSRKWGPFMKNMKNFLSPYYDLSMPDQHIAKILKNVGFKNIDVRSKQKMYTFKNLEKFRGLMIGVNPFKVPENEFENYIDDLMETARTLRIIDEENGTLSFLFNMNIVHCTK

Description

O-methyltransferase that transfers a methyl group from S-adenosyl-L-methionine (SAM) to the carboxyl group of juvenile hormone acids to produce active juvenile hormones in the corpora allata, the last step during juvenile hormone biosynthesis (PubMed:14530389). Also able to methylate farnesoate to methyl farnesoate (PubMed:14530389).
O-methyltransferase that transfers a methyl group from S-adenosyl-L-methionine (SAM) to the carboxyl group of juvenile hormone acids to produce active juvenile hormones in the corpora allata, the last step during juvenile hormone biosynthesis (PubMed:18549957). Also able to methylate farnesoate to methyl farnesoate (PubMed:18549957).

Catalytic Activity

(2E,6E)-farnesoate + S-adenosyl-L-methionine = methyl (2E,6E)-farnesoate + S-adenosyl-L-homocysteine
juvenile hormone III carboxylate + S-adenosyl-L-methionine = juvenile hormone III + S-adenosyl-L-homocysteine

Biophysicochemical Properties

3.32 uM for juvenile hormone III acid
0.18 uM for farnesoate

Similarity

Belongs to the methyltransferase superfamily.

Keywords

Complete proteome   Methyltransferase   Reference proteome   S-adenosyl-L-methionine   Transferase  

Feature

chain  Juvenile hormone acid O-methyltransferase

Uniprot

H9JLJ1   H9JWV0   Q1KN64   A5LFV6   Q9U4Z8   A0A194R0H9   + More
A0A173GZJ8   D2Y4U6   Q767F1   A0A0R5ZNF1   A0A3S2M9U8   A0A194PH70   A0A1P1ENW6   A0A2A4K0R4   A0A2H1VCR6   A0A212F9H0   A0A1W5ZM45   A0A2A4JYY3   A5LFV5   A0A2H1VCN4   A0A0L7L836   B2DCQ0   B2DCP9   A0A0H4SZT2   A0A1I8MHF6   A0A024BT15   A0A139WJN5   A0A0L0C8K2   A0A1I8NU96   B2DCP8   A0A1I8PV71   A0A146IWP0   A0A1I8MHL4   A0A1A9WKH5   A0A1I8MGC1   A0A2J7RC57   Q206L4   A0A1B0D0M7   A0A1I8MHJ3   A0A1A9Z807   A0A087TP03   A0A2C9DK13   A0A075W3Z0   A0A1B0G4E2   A0A1B0CX51   A0A1A9VCC9   T1GFV4   R4IKD3   A0A1W4WX71   B4LVM0   B4G7P2   A0A1A9XET3   A0A1B0BCZ1   Q29LS0   B4MZR7   A0A3B0K301   A0A1I8MH96   A0A1W4VSL6   A0A2C8C2A6   B4I231   Q9VJK8   B4P880   A0A1I8P888   A0A1I8NP59   A0A2R4N6F4   A0A336L491   A0A146KYR9   A0A182PFZ1   A0A0A9XHP0   A0A034W1Z2   A0A1I8MNI4   A0A0J9TPF7   A0A0K8S8Y3   A0A3S5X9D4   A0A182M749   A0A084VUE9   B4KKK5   A0A1W4VJN5   A0A1A9WKH6   A0A182N9N0   A0A146J3Z5   A0A1I8Q8P6   A0A1I8MED4   E0VP25   A0A1I8PAX7   A0A182RP42   A0A182ILY4   A0A182T4X4   A0A1A9Z806   B3MUK9   A0A0A1WN75   A0A1B3PDM1   B4JZ63   A0A182KF39   A0A1L2BNI6   B3NNG6   A0A1I8NYM0  

EC Number

2.1.1.325

Pubmed

19121390    18839418    10620045    26354079    14530389    22118469    + More
28382786    26227816    18435763    18362917    19820115    25315136    26108605    17510324    28536442    23499946    17994087    15632085    18549957    10731132    12537572    12537569    17550304    26823975    25401762    25348373    22936249    24438588    26894583    20566863    25830018   

EMBL

BABH01005671    BABH01018740    DQ465408    ABE98256.1    AB127945    BAF63630.1    + More
AF117590    AAF16712.1    KQ460883    KPJ11207.1    KX289532    ANI21405.1    GU323798    ADA83703.1    AB113578    JN851817    KM926339    AJR27469.1    RSAL01000003    RVE54742.1    KQ459603    KPI92751.1    BABH01005674    NWSH01000345    PCG77232.1    ODYU01001839    SOQ38586.1    AGBW02009587    OWR50378.1    KY421777    ARI71313.1    PCG77231.1    AB127944    BAF63629.1    SOQ38587.1    JTDY01002391    KOB71509.1    AB360763    KQ971338    BAG30999.1    EFA02917.1    AB360762    BAG30998.1    KP274881    AKQ00043.1    KJ188030    AHZ20738.1    KYB28190.1    JRES01000751    KNC28768.1    AB360761    BAG30997.1    LC049616    BAU78328.1    NEVH01005887    PNF38426.1    DQ409061    CH477383    ABD65474.1    EAT42177.1    AJVK01009988    KK116107    KFM66842.1    KX880373    ARE68679.1    KM212828    AIG94926.1    CCAG010005611    AJWK01033250    CAQQ02017873    CAQQ02017874    CAQQ02017875    CAQQ02017876    CAQQ02017877    CAQQ02017878    JX291120    AFQ01089.1    CH940649    EDW64414.1    CH479180    EDW29313.1    JXJN01012210    CH379060    EAL33975.2    CH963920    EDW77852.1    OUUW01000004    SPP79391.1    LT716988    LT716989    LT716990    SIW59357.1    SIW59358.1    SIW59359.1    CH480820    EDW54588.1    AB113579    AE014134    AY075194    CM000158    EDW90127.1    MG269999    AVX28147.1    UFQS01000997    UFQT01000997    SSX08344.1    SSX28367.1    GDHC01017106    JAQ01523.1    GBHO01024463    GBHO01010623    GDHC01003517    JAG19141.1    JAG32981.1    JAQ15112.1    GAKP01009356    JAC49596.1    CM002910    KMY90515.1    GBRD01016222    JAG49604.1    KT869353    ALO17567.1    AXCM01012846    ATLV01016737    KE525103    KFB41593.1    CH933807    EDW11655.1    LC055463    BAU79434.1    DS235354    EEB15131.1    CH902624    EDV33538.1    GBXI01014449    JAC99842.1    KU660037    AOG17835.1    CH916379    EDV94172.1    KU159280    ALT10380.1    CH954179    EDV56617.1   

Proteomes

UP000005204    UP000053240    UP000283053    UP000053268    UP000218220    UP000007151    + More
UP000037510    UP000007266    UP000095301    UP000037069    UP000095300    UP000091820    UP000235965    UP000008820    UP000092462    UP000092445    UP000054359    UP000079169    UP000092444    UP000092461    UP000078200    UP000015102    UP000192223    UP000008792    UP000008744    UP000092443    UP000092460    UP000001819    UP000007798    UP000268350    UP000192221    UP000001292    UP000000803    UP000002282    UP000075885    UP000075883    UP000030765    UP000009192    UP000075884    UP000009046    UP000075900    UP000075880    UP000075901    UP000007801    UP000001070    UP000075881    UP000008711   

PRIDE

H9JLJ1    H9JWV0    Q767F1    Q9VJK8   

Pfam

PF13847   Methyltransf_31        + More
PF08241   Methyltransf_11
PF13649   Methyltransf_25
PF08242   Methyltransf_12
PF00071   Ras

Interpro

IPR029063   SAM-dependent_MTases        + More
IPR025714   Methyltranfer_dom       
IPR013216   Methyltransf_11       
IPR041698   Methyltransf_25       
IPR013217   Methyltransf_12       
IPR027417   P-loop_NTPase       
IPR001806   Small_GTPase       
IPR037872   Rab3       
IPR005225   Small_GTP-bd_dom       

SUPFAM

SSF53335   SSF53335        + More
SSF52540   SSF52540       

CDD

cd01865  Rab3

ProteinModelPortal

H9JLJ1   H9JWV0   Q1KN64   A5LFV6   Q9U4Z8   A0A194R0H9   + More
A0A173GZJ8   D2Y4U6   Q767F1   A0A0R5ZNF1   A0A3S2M9U8   A0A194PH70   A0A1P1ENW6   A0A2A4K0R4   A0A2H1VCR6   A0A212F9H0   A0A1W5ZM45   A0A2A4JYY3   A5LFV5   A0A2H1VCN4   A0A0L7L836   B2DCQ0   B2DCP9   A0A0H4SZT2   A0A1I8MHF6   A0A024BT15   A0A139WJN5   A0A0L0C8K2   A0A1I8NU96   B2DCP8   A0A1I8PV71   A0A146IWP0   A0A1I8MHL4   A0A1A9WKH5   A0A1I8MGC1   A0A2J7RC57   Q206L4   A0A1B0D0M7   A0A1I8MHJ3   A0A1A9Z807   A0A087TP03   A0A2C9DK13   A0A075W3Z0   A0A1B0G4E2   A0A1B0CX51   A0A1A9VCC9   T1GFV4   R4IKD3   A0A1W4WX71   B4LVM0   B4G7P2   A0A1A9XET3   A0A1B0BCZ1   Q29LS0   B4MZR7   A0A3B0K301   A0A1I8MH96   A0A1W4VSL6   A0A2C8C2A6   B4I231   Q9VJK8   B4P880   A0A1I8P888   A0A1I8NP59   A0A2R4N6F4   A0A336L491   A0A146KYR9   A0A182PFZ1   A0A0A9XHP0   A0A034W1Z2   A0A1I8MNI4   A0A0J9TPF7   A0A0K8S8Y3   A0A3S5X9D4   A0A182M749   A0A084VUE9   B4KKK5   A0A1W4VJN5   A0A1A9WKH6   A0A182N9N0   A0A146J3Z5   A0A1I8Q8P6   A0A1I8MED4   E0VP25   A0A1I8PAX7   A0A182RP42   A0A182ILY4   A0A182T4X4   A0A1A9Z806   B3MUK9   A0A0A1WN75   A0A1B3PDM1   B4JZ63   A0A182KF39   A0A1L2BNI6   B3NNG6   A0A1I8NYM0  

PDB

2PJD     E-value=0.00316976,     Score=94

KEGG

101743036    K10718 juvenile hormone-III synthase [EC:2.1.1.325] | (RefSeq) uncharacterized LOC101743036

PATHWAY

00981    Insect hormone biosynthesis - Bombyx mori (domestic silkworm)

GO

GO:0008168   GO:0006718   GO:0035049   GO:0032259   GO:0019010   GO:0016740   GO:0016021   GO:0008049   GO:0003924   GO:0005525  

Length:

266

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00261

Exp number, first 60 AAs:

0.00131

Total prob of N-in:

0.04037

outside

1  -  266

 

 

Pi

244.897787

Theta

161.040036

Tajima's D

1.783109

CLR

0.321486

CSRT

0.842057897105145

Interpretation

Uncertain