Gene

KWMTBOMO07182

Pre Gene Modal

BGIBMGA010393

Annotation

PREDICTED:_uncharacterized_protein_LOC101743036_[Bombyx_mori]

Location

Bomo_Chr12(-):7217991-7224721      View in EpiBrowser

Full name

Juvenile hormone acid O-methyltransferase      

Alternative Name

Juvenile hormone acid methyltransferase

Location in the cell

Cytoplasmic   Reliability : 2.27

CDS

ATGTGGAACAATGCGGAGATTTATCATGAAAATAACAGTATTACGAGACGAGATGCAATAGAATCACTAGATGCATTCGCTCCATTGTTCAACTGGAAATCCAATGAAAAAATCTTGGAAGTGGGATGTGCGGATGGAAGCATCACTAAAATTTTAGCAACATATTTCCCGAAGAACATACACTCGCTAACCGCATGTGACAATGACAAAAAAGGACTTAAGTATGCCAGTGAACATAATAAGAATGAGAAGATCACTTTTAAATTTATGGACATTGAAGAGGATTTGTCCAGTGATGTTAAAGGAAAATTCGATCACGTATTTTCGTTGTGCACCTTCCATTGGATCAGGAAGCAACAAAAAGCTTTCCAAAACATCTATGATCTTCTTACTACTGAAGGGGAATGTTTTCTGACAATGTTTGCTTATACACAAATGTATCACTTTTATGTGATTTTGAAACATTCTGAAAAATGGAGAGCATGGCTACAAGATATGACTATGTTTTTGTCACCATACTATAACATTTCGAGTCCAGATAATTTAGTTGAAGAAATGTTGAAAAAGATTGGATTTAAACACGTCCAAATTCAAACGAAACAAAAGACTTACTGTTTCCAAAAACAGGAATTTCGAGAAATTATGAGAGCAGTGAATCCTTTCAAGATCCCGAATGAGAAGATTGAACACTTTATTGACGATTTGTTCGAAGCGGCACGTGGAATGGCCCTCATCAAAGAAGACGGAACCCTTGATGCCACATACAACTTACTTATTATTTATTGTCAGAAATAA

Protein

MWNNAEIYHENNSITRRDAIESLDAFAPLFNWKSNEKILEVGCADGSITKILATYFPKNIHSLTACDNDKKGLKYASEHNKNEKITFKFMDIEEDLSSDVKGKFDHVFSLCTFHWIRKQQKAFQNIYDLLTTEGECFLTMFAYTQMYHFYVILKHSEKWRAWLQDMTMFLSPYYNISSPDNLVEEMLKKIGFKHVQIQTKQKTYCFQKQEFREIMRAVNPFKIPNEKIEHFIDDLFEAARGMALIKEDGTLDATYNLLIIYCQK

Description

O-methyltransferase that transfers a methyl group from S-adenosyl-L-methionine (SAM) to the carboxyl group of juvenile hormone acids to produce active juvenile hormones in the corpora allata, the last step during juvenile hormone biosynthesis (PubMed:14530389). Also able to methylate farnesoate to methyl farnesoate (PubMed:14530389).
O-methyltransferase that transfers a methyl group from S-adenosyl-L-methionine (SAM) to the carboxyl group of juvenile hormone acids to produce active juvenile hormones in the corpora allata, the last step during juvenile hormone biosynthesis (PubMed:18549957). Also able to methylate farnesoate to methyl farnesoate (PubMed:18549957).

Catalytic Activity

(2E,6E)-farnesoate + S-adenosyl-L-methionine = methyl (2E,6E)-farnesoate + S-adenosyl-L-homocysteine
juvenile hormone III carboxylate + S-adenosyl-L-methionine = juvenile hormone III + S-adenosyl-L-homocysteine

Biophysicochemical Properties

3.32 uM for juvenile hormone III acid
0.18 uM for farnesoate

Similarity

Belongs to the methyltransferase superfamily.

Keywords

Complete proteome   Methyltransferase   Reference proteome   S-adenosyl-L-methionine   Transferase  

Feature

chain  Juvenile hormone acid O-methyltransferase

Uniprot

H9JLJ1   H9JWV0   Q767F1   A0A1P1ENW6   A0A2A4K0R4   A0A212F9H0   + More
A0A194PH70   A0A194R0H9   A0A2H1VCR6   Q1KN64   A5LFV6   A0A173GZJ8   A0A3S2M9U8   Q9U4Z8   D2Y4U6   A0A1W5ZM45   A0A0R5ZNF1   A0A2A4JYY3   A0A2H1VCN4   A5LFV5   A0A0L7L836   A0A2C9DK13   Q206L4   A0A075W3Z0   A0A182ILY4   A0A1I8NU96   A0A084VUE9   A0A0L0C8K2   A0A182M749   A0A336L491   B4P880   A0A1I8MHF6   A0A182PFZ1   A0A1W4VJN5   B3NNG6   A0A0J9TPF7   A0A2M4ALK9   A0A1A9WKH5   B4I231   A0A182RP42   A0A3B0K301   B4LVM0   A0A182KF39   A0A1B0G4E2   A0A1W4VSL6   A0A182YBZ0   B4G7P2   Q9VJK8   A0A182T4X4   T1IRK8   Q29LS0   A0A1B0D0M7   R4IKD3   A0A2M3Z4F9   B2DCQ0   A0A2M3Z4G9   A0A0K8TS48   A0A1A9VCC9   A0A182HNS9   Q7Q9F0   A0A1A9WKH6   A0A2M3Z4E3   A0A1A9Z807   A0A182FCT5   A0A1L2BNI6   A0A2J7RC57   A0A3S5X9D4   A0A2M4BW66   A0A182WMH0   A0A146KYR9   A0A0H4SZT2   A0A146IWP0   W8BZU3   B4KKK5   A0A0A9XHP0   A0A2M4BVZ2   A0A1B3PD94   A0A2M4BWC8   A0A0K8S8Y3   B3MUK9   A0A182N9N0   A0A1I8MED4   W5JAE4   A0A1J0I2X3   T1GFV4   A0A1W4WX71   A0A1A9XET3   A0A1B0BCZ1   A0A1A9Z806   B4MZR7   B4JZ63   A0A1A9XET4   A0A1A9VS52   A0A182X8H7   A0A0M3QU49   A0A182LGS6   A0A1B3PDM2   A0A1B0CX51  

EC Number

2.1.1.325

Pubmed

19121390    14530389    22118469    26354079    18839418    10620045    + More
28382786    26227816    28536442    17510324    24438588    26108605    17994087    17550304    25315136    22936249    25244985    18549957    10731132    12537572    12537569    15632085    23499946    18435763    18362917    19820115    26369729    12364791    14747013    17210077    26823975    24495485    25401762    20920257    23761445    20966253   

EMBL

BABH01005671    BABH01018740    AB113578    JN851817    BABH01005674    NWSH01000345    + More
PCG77232.1    AGBW02009587    OWR50378.1    KQ459603    KPI92751.1    KQ460883    KPJ11207.1    ODYU01001839    SOQ38586.1    DQ465408    ABE98256.1    AB127945    BAF63630.1    KX289532    ANI21405.1    RSAL01000003    RVE54742.1    AF117590    AAF16712.1    GU323798    ADA83703.1    KY421777    ARI71313.1    KM926339    AJR27469.1    PCG77231.1    SOQ38587.1    AB127944    BAF63629.1    JTDY01002391    KOB71509.1    KX880373    ARE68679.1    DQ409061    CH477383    ABD65474.1    EAT42177.1    KM212828    AIG94926.1    ATLV01016737    KE525103    KFB41593.1    JRES01000751    KNC28768.1    AXCM01012846    UFQS01000997    UFQT01000997    SSX08344.1    SSX28367.1    CM000158    EDW90127.1    CH954179    EDV56617.1    CM002910    KMY90515.1    GGFK01008333    MBW41654.1    CH480820    EDW54588.1    OUUW01000004    SPP79391.1    CH940649    EDW64414.1    CCAG010005611    CH479180    EDW29313.1    AB113579    AE014134    AY075194    JH431370    CH379060    EAL33975.2    AJVK01009988    JX291120    AFQ01089.1    GGFM01002660    MBW23411.1    AB360763    KQ971338    BAG30999.1    EFA02917.1    GGFM01002642    MBW23393.1    GDAI01000627    JAI16976.1    APCN01002761    AAAB01008900    EAA09331.1    GGFM01002633    MBW23384.1    KU159280    ALT10380.1    NEVH01005887    PNF38426.1    KT869353    ALO17567.1    GGFJ01008101    MBW57242.1    GDHC01017106    JAQ01523.1    KP274881    AKQ00043.1    LC049616    BAU78328.1    GAMC01001658    JAC04898.1    CH933807    EDW11655.1    GBHO01024463    GBHO01010623    GDHC01003517    JAG19141.1    JAG32981.1    JAQ15112.1    GGFJ01008104    MBW57245.1    KU659901    AOG17700.1    GGFJ01008100    MBW57241.1    GBRD01016222    JAG49604.1    CH902624    EDV33538.1    ADMH02002051    ETN59754.1    KT947473    APC62652.1    CAQQ02017873    CAQQ02017874    CAQQ02017875    CAQQ02017876    CAQQ02017877    CAQQ02017878    JXJN01012210    CH963920    EDW77852.1    CH916379    EDV94172.1    CP012523    ALC40009.1    KU660028    AOG17826.1    AJWK01033250   

Proteomes

UP000005204    UP000218220    UP000007151    UP000053268    UP000053240    UP000283053    + More
UP000037510    UP000008820    UP000079169    UP000075880    UP000095300    UP000030765    UP000037069    UP000075883    UP000002282    UP000095301    UP000075885    UP000192221    UP000008711    UP000091820    UP000001292    UP000075900    UP000268350    UP000008792    UP000075881    UP000092444    UP000076408    UP000008744    UP000000803    UP000075901    UP000001819    UP000092462    UP000007266    UP000078200    UP000075840    UP000007062    UP000092445    UP000069272    UP000235965    UP000075920    UP000009192    UP000007801    UP000075884    UP000000673    UP000015102    UP000192223    UP000092443    UP000092460    UP000007798    UP000001070    UP000076407    UP000092553    UP000075882    UP000092461   

PRIDE

H9JLJ1    H9JWV0    Q767F1    Q9VJK8   

Pfam

PF13847   Methyltransf_31        + More
PF08241   Methyltransf_11
PF13649   Methyltransf_25
PF08242   Methyltransf_12
PF00071   Ras

Interpro

IPR029063   SAM-dependent_MTases        + More
IPR025714   Methyltranfer_dom       
IPR013216   Methyltransf_11       
IPR041698   Methyltransf_25       
IPR013217   Methyltransf_12       
IPR001806   Small_GTPase       
IPR027417   P-loop_NTPase       
IPR037872   Rab3       
IPR005225   Small_GTP-bd_dom       

SUPFAM

SSF53335   SSF53335        + More
SSF52540   SSF52540       

CDD

cd01865  Rab3

ProteinModelPortal

H9JLJ1   H9JWV0   Q767F1   A0A1P1ENW6   A0A2A4K0R4   A0A212F9H0   + More
A0A194PH70   A0A194R0H9   A0A2H1VCR6   Q1KN64   A5LFV6   A0A173GZJ8   A0A3S2M9U8   Q9U4Z8   D2Y4U6   A0A1W5ZM45   A0A0R5ZNF1   A0A2A4JYY3   A0A2H1VCN4   A5LFV5   A0A0L7L836   A0A2C9DK13   Q206L4   A0A075W3Z0   A0A182ILY4   A0A1I8NU96   A0A084VUE9   A0A0L0C8K2   A0A182M749   A0A336L491   B4P880   A0A1I8MHF6   A0A182PFZ1   A0A1W4VJN5   B3NNG6   A0A0J9TPF7   A0A2M4ALK9   A0A1A9WKH5   B4I231   A0A182RP42   A0A3B0K301   B4LVM0   A0A182KF39   A0A1B0G4E2   A0A1W4VSL6   A0A182YBZ0   B4G7P2   Q9VJK8   A0A182T4X4   T1IRK8   Q29LS0   A0A1B0D0M7   R4IKD3   A0A2M3Z4F9   B2DCQ0   A0A2M3Z4G9   A0A0K8TS48   A0A1A9VCC9   A0A182HNS9   Q7Q9F0   A0A1A9WKH6   A0A2M3Z4E3   A0A1A9Z807   A0A182FCT5   A0A1L2BNI6   A0A2J7RC57   A0A3S5X9D4   A0A2M4BW66   A0A182WMH0   A0A146KYR9   A0A0H4SZT2   A0A146IWP0   W8BZU3   B4KKK5   A0A0A9XHP0   A0A2M4BVZ2   A0A1B3PD94   A0A2M4BWC8   A0A0K8S8Y3   B3MUK9   A0A182N9N0   A0A1I8MED4   W5JAE4   A0A1J0I2X3   T1GFV4   A0A1W4WX71   A0A1A9XET3   A0A1B0BCZ1   A0A1A9Z806   B4MZR7   B4JZ63   A0A1A9XET4   A0A1A9VS52   A0A182X8H7   A0A0M3QU49   A0A182LGS6   A0A1B3PDM2   A0A1B0CX51  

PDB

4KRI     E-value=0.00770756,     Score=90

KEGG

101745533    K10718 juvenile hormone-III synthase [EC:2.1.1.325] | (RefSeq) uncharacterized LOC101745533

PATHWAY

00981    Insect hormone biosynthesis - Bombyx mori (domestic silkworm)

GO

GO:0006718   GO:0035049   GO:0032259   GO:0019010   GO:0008168   GO:0016021   GO:0016740   GO:0008049   GO:0005525   GO:0003924  

Length:

264

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.45142

Exp number, first 60 AAs:

0.00161

Total prob of N-in:

0.10358

outside

1  -  264

 

 

Pi

339.912346

Theta

146.097629

Tajima's D

5.468655

CLR

0.25163

CSRT

0.999950002499875

Interpretation

Uncertain