Gene

KWMTBOMO07133  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA010546

Annotation

prophenoloxidase_activating_enzyme_[Helicoverpa_armigera]

Location

Bomo_Chr12(+):5930072-5932271      View in EpiBrowser

Full name

Serine protease 7       + More
Phenoloxidase-activating factor 1      
CLIP domain-containing serine protease 2      

Alternative Name

Melanization protease 2
Prophenoloxidase-activating factor I
Serine protease PPAF-1
BzArgOEtase

Location in the cell

Extracellular   Reliability : 1.989

CDS

ATGCGTTGCGGTTTGAAGACTACGGTTCGTCTTGGCGAATACGACACTCAATCTGACCGGGATTGTGTTGACAATGTGTGCGCGGATCCGCCGCAAGACATCCCAGTACTTGCCGTCTATCCTCATCAGGGATTTAGCGACCAGAATAAGAACAGAAAAGATGATATTGGTTTAATTCGATTGGCGAGGAGAGTTACCTATTCATATTACGTTCAACCTATCTGCCTCGTCGAACCGAACGGTCGATTGGACGTAGGTAATGACGTTTTCGTTGCGGGCTGGGGGAAAACCTTAAACGGTCGCAATAGCCCAGTCAAATTAAAACTCAATGTTCCAATATTCGCAAAGAATGATTGCGCCTCAAAATATAGACAATTAGGAGTTGATTTGACTGATAGGCAGATATGTGCTGGTGGTGTATTCGCCGAAGACGCCTGTAGAGGAGACTCCGGCGGACCTCTTATGTTGAGGAGACCTGATGGCGTTTGGGAAACAGTTGCAGTTGTCTCATTTGGTTATGGCTGCGGACGTGATGGATGGCCAGGAGTTTACACTTCGGTAGCTAATTACATAGATTGGATCGAGTCGACTTTGCAATCGTCGAACGAGTGA

Protein

MRCGLKTTVRLGEYDTQSDRDCVDNVCADPPQDIPVLAVYPHQGFSDQNKNRKDDIGLIRLARRVTYSYYVQPICLVEPNGRLDVGNDVFVAGWGKTLNGRNSPVKLKLNVPIFAKNDCASKYRQLGVDLTDRQICAGGVFAEDACRGDSGGPLMLRRPDGVWETVAVVSFGYGCGRDGWPGVYTSVANYIDWIESTLQSSNE

Description

Serine protease which, by cleaving and activating prophenoloxidase (PPO1) after immune challenge, plays an essential role in the melanization immune response to septic wounding (PubMed:16256951, PubMed:16322759, PubMed:19071960, PubMed:24260243, PubMed:16861233). May function in diverse Hayan-dependent PPO1-activating cascades that are negatively controlled by different serpin proteins; Spn27A in the hemolymph and Spn77BA in the trachea (PubMed:18854145, PubMed:24260243, PubMed:16322759). Important for the innate immune response to fungi (PubMed:16861233). Regulation of melanization and PPO1 activation appears to be largely independent of the Toll signaling pathway (PubMed:16861233).
Serine endopeptidase which, by cleaving prophenoloxidase PPO1 and PPO2, is required for the activation of the prophenoloxidase cascade probably following the recognition of pathogen-derived products.
Endopeptidase with selective post-Arg cleavage site (PubMed:16399077, PubMed:7737188). Might play a role in innate immunity in response to fungal and Gram-positive bacterial infections (PubMed:16399077).

Subunit

Interacts with Spn27A.
In the active form, heterodimer of a light chain and a heavy chain; disulfide-linked (Probable).
In the active form, heterodimer of a light chain and a heavy chain; disulfide-linked.

Similarity

Belongs to the peptidase S1 family.
Belongs to the peptidase S1 family. CLIP subfamily.

Keywords

Calcium   Complete proteome   Disulfide bond   Glycoprotein   Hydrolase   Metal-binding   Protease   Reference proteome   Secreted   Serine protease   Signal   Zymogen   3D-structure   Direct protein sequencing   Immunity   Innate immunity   Cleavage on pair of basic residues  

Feature

propeptide  Activation peptide
chain  Serine protease 7

Uniprot

H9JLZ4   A0A2H1V1Q2   S4PK70   A0A2A4JQA1   A0A290U615   B3F5B1   + More
O77102   Q5DI99   Q49QW1   D5LPT4   A0A0U1WT79   A0A173GPF2   A0A194R0R2   I4DN03   I4DJ03   A0A3S2M731   A0A212EKT5   A0A0L7LLI6   A0A194PP07   Q9NB86   A0A173M1W2   A0A139WN30   A0A1A9W879   D6W9Z5   A0A1Y1KJG0   A0A1A9Z167   A0A0A1XPG9   A0A1B0FQW4   A0A1A9X740   A0A2D0XV03   A0A1A9VW86   A0A1Y1KP79   B4N8B1   Q9V3Z2   A0A1W4VMD7   B3NYQ7   W8BLL3   B0WA88   U5ESH9   A0A0K8W2H3   J3JXM3   A0A1Q3FEM6   B4I4W4   B4QZQ4   A0A3B0JPE4   B5DWB5   Q294N1   B4PRF7   B4GMV5   W8BSR7   A0A0A1WGL4   A0A1J1I303   A0A023EJG2   A0A0L0CE89   B4JF72   A0A2H1VCQ7   A0A1I8PP27   B3LYY3   B4K6S5   K7J980   E9IPW8   A0A034WLR0   B4M4J5   A0A1Y1NHS8   B1B5K1   A0A0K8VB28   A0A0M9A7E2   I4DND8   A0A1W4XI92   A0A034V075   A0A1I8N0W4   O97366   A0A194RQ53   A0A023EQB0   Q2VG86   A0A182GNZ3   A0A2A4IX69   A0A023EPR7   A0A023EQV6   A0A182G580   A0A0M4ERW8   A0A0C9QZC0   A0A1W7R8M0   A0A1S4F560   Q17B76   A0A0K8VIH4   A0A0K8UPH0   A0A182G5Z0   Q17EX4   D6WUF6   A0A1Y1K9U3   A0A290U621   A0A0M4EP76   A0A026WIN7   E2C8L2   Q5MPC6   A0A151WR74   A0A3L8D7J0   A0A2A3EA55   B4NGU2  

EC Number

3.4.21.-

Pubmed

19121390    23622113    28953952    9770467    15857768    18207075    + More
19557749    26354079    22651552    22118469    26227816    11267903    18362917    19820115    28004739    25830018    28990217    17994087    10731132    12537572    15579698    16256951    16322759    16861233    18854145    19071960    22227521    24260243    24495485    22516182    15632085    17550304    24945155    26108605    20075255    21282665    25348373    18195005    25315136    9839951    16362048    9652393    11012672    12185078    17287215    16399077    7737188    26483478    17510324    28756777    24508170    20798317    15944088    30249741   

EMBL

BABH01005537    BABH01005538    ODYU01000283    SOQ34775.1    GAIX01004665    JAA87895.1    + More
NWSH01000833    PCG73946.1    KY680243    ATD13324.1    EF614332    ABU98654.1    AF059728    AAC64004.1    AY789465    AM293327    AAX18636.1    CAL25132.1    AY677081    AAW24480.1    GU953224    ADF43208.1    JQ581597    AFX82593.1    KT006136    ANH58161.1    KQ460883    KPJ11099.1    AK402671    BAM19293.1    AK401271    BAM17893.1    RSAL01000017    RVE52975.1    AGBW02014220    OWR42068.1    JTDY01000666    KOB76290.1    KQ459603    KPI92860.1    AF261976    AAF74738.1    LC150880    BAV01246.1    KQ971312    KYB29266.1    EEZ99203.2    GEZM01081962    JAV61464.1    GBXI01001381    JAD12911.1    CCAG010023843    KX346579    ASN76826.1    GEZM01081961    JAV61465.1    CH964232    EDW81362.1    AE014297    AF233093    AY665379    BT128742    AAF43410.1    AAT76546.1    AEH59645.1    CH954181    EDV48170.1    GAMC01016016    JAB90539.1    DS231869    EDS40917.1    GANO01002346    JAB57525.1    GDHF01006992    JAI45322.1    BT127992    AEE62954.1    GFDL01009047    JAV25998.1    CH480821    EDW55257.1    CM000364    EDX12012.1    OUUW01000008    SPP84087.1    CM000070    EDY68533.1    EAL28934.1    CM000160    EDW96345.1    CH479185    EDW38179.1    GAMC01002145    JAC04411.1    GBXI01016739    GBXI01006351    JAC97552.1    JAD07941.1    CVRI01000039    CRK94579.1    GAPW01004503    JAC09095.1    JRES01000503    KNC30561.1    CH916369    EDV93353.1    ODYU01001850    SOQ38623.1    CH902617    EDV41857.1    CH933806    EDW14191.1    AAZX01002213    GL764655    EFZ17385.1    GAKP01004269    JAC54683.1    CH940652    EDW59556.1    GEZM01002864    JAV97108.1    AB363980    BAG14262.2    GDHF01016160    JAI36154.1    KQ435719    KOX78755.1    AK402861    KQ459604    BAM19428.1    KPI92345.1    GAKP01023416    JAC35542.1    AB013088    BAA34642.1    KQ460045    KPJ18151.1    GAPW01001836    JAC11762.1    AB035418    AY061936    DQ157441    BABH01019990    AAL31707.1    ABB58762.1    BAE73254.1    JXUM01015012    JXUM01015013    KQ560420    KXJ82504.1    NWSH01005677    PCG64066.1    GAPW01002593    JAC11005.1    GAPW01002093    JAC11505.1    JXUM01042955    JXUM01042956    KQ561344    KXJ78893.1    CP012526    ALC47976.1    GBYB01001065    JAG70832.1    GEHC01000168    JAV47477.1    CH477325    EAT43491.1    GDHF01013623    JAI38691.1    GDHF01024094    GDHF01019994    JAI28220.1    JAI32320.1    JXUM01044209    JXUM01044210    KQ561391    KXJ78725.1    CH477278    EAT45085.1    KQ971352    EFA07558.1    GEZM01092820    JAV56445.1    KY680242    KZ150014    ATD13323.1    PZC75014.1    ALC47552.1    KK107185    EZA55838.1    GL453666    EFN75742.1    AY672784    AAV91006.1    KQ982813    KYQ50307.1    QOIP01000012    RLU16063.1    KZ288322    PBC28172.1    CH964272    EDW84439.2   

Proteomes

UP000005204    UP000218220    UP000053240    UP000283053    UP000007151    UP000037510    + More
UP000053268    UP000007266    UP000091820    UP000092445    UP000092444    UP000092443    UP000078200    UP000007798    UP000000803    UP000192221    UP000008711    UP000002320    UP000001292    UP000000304    UP000268350    UP000001819    UP000002282    UP000008744    UP000183832    UP000037069    UP000001070    UP000095300    UP000007801    UP000009192    UP000002358    UP000008792    UP000053105    UP000192223    UP000095301    UP000069940    UP000249989    UP000092553    UP000008820    UP000053097    UP000008237    UP000075809    UP000279307    UP000242457   

PRIDE

H9JLZ4    Q9V3Z2    Q2VG86   

Pfam

PF00089   Trypsin        + More
PF12032   CLIP
PF13019   Sde2_N_Ubi

Interpro

IPR001314   Peptidase_S1A        + More
IPR009003   Peptidase_S1_PA       
IPR018114   TRYPSIN_HIS       
IPR022700   CLIP       
IPR038565   CLIP_sf       
IPR001254   Trypsin_dom       
IPR033116   TRYPSIN_SER       
IPR024974   Sde2_N       

SUPFAM

SSF50494   SSF50494       

Gene 3D

2.20.20.90    

CDD

cd00190  Tryp_SPc

ProteinModelPortal

H9JLZ4   A0A2H1V1Q2   S4PK70   A0A2A4JQA1   A0A290U615   B3F5B1   + More
O77102   Q5DI99   Q49QW1   D5LPT4   A0A0U1WT79   A0A173GPF2   A0A194R0R2   I4DN03   I4DJ03   A0A3S2M731   A0A212EKT5   A0A0L7LLI6   A0A194PP07   Q9NB86   A0A173M1W2   A0A139WN30   A0A1A9W879   D6W9Z5   A0A1Y1KJG0   A0A1A9Z167   A0A0A1XPG9   A0A1B0FQW4   A0A1A9X740   A0A2D0XV03   A0A1A9VW86   A0A1Y1KP79   B4N8B1   Q9V3Z2   A0A1W4VMD7   B3NYQ7   W8BLL3   B0WA88   U5ESH9   A0A0K8W2H3   J3JXM3   A0A1Q3FEM6   B4I4W4   B4QZQ4   A0A3B0JPE4   B5DWB5   Q294N1   B4PRF7   B4GMV5   W8BSR7   A0A0A1WGL4   A0A1J1I303   A0A023EJG2   A0A0L0CE89   B4JF72   A0A2H1VCQ7   A0A1I8PP27   B3LYY3   B4K6S5   K7J980   E9IPW8   A0A034WLR0   B4M4J5   A0A1Y1NHS8   B1B5K1   A0A0K8VB28   A0A0M9A7E2   I4DND8   A0A1W4XI92   A0A034V075   A0A1I8N0W4   O97366   A0A194RQ53   A0A023EQB0   Q2VG86   A0A182GNZ3   A0A2A4IX69   A0A023EPR7   A0A023EQV6   A0A182G580   A0A0M4ERW8   A0A0C9QZC0   A0A1W7R8M0   A0A1S4F560   Q17B76   A0A0K8VIH4   A0A0K8UPH0   A0A182G5Z0   Q17EX4   D6WUF6   A0A1Y1K9U3   A0A290U621   A0A0M4EP76   A0A026WIN7   E2C8L2   Q5MPC6   A0A151WR74   A0A3L8D7J0   A0A2A3EA55   B4NGU2  

PDB

2OLG     E-value=7.10675e-42,     Score=425

KEGG

101739185    no KO assigned | (RefSeq) serine protease easter-like

GO

GO:0004252   GO:0008236   GO:0035006   GO:0005576   GO:0006508   GO:0035008   GO:0046872   GO:0008233   GO:0045087   GO:0004175   GO:0016021   GO:0005615   GO:0006468   GO:0005515   GO:0031032   GO:0030060   GO:0019752   GO:0016491   GO:0016295   GO:0016296  

Subcellular location

Secreted  

Length:

203

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.01573

Exp number, first 60 AAs:

0.00017

Total prob of N-in:

0.28753

outside

1  -  203

 

 

Pi

126.347952

Theta

141.898705

Tajima's D

-0.588389

CLR

0

CSRT

0.224038798060097

Interpretation

Uncertain