Gene

KWMTBOMO07018

Pre Gene Modal

BGIBMGA010465

Annotation

PREDICTED:_neutral_ceramidase_[Amyelois_transitella]

Location

Bomo_Chr12(-):1909459-1934203      View in EpiBrowser

Full name

Neutral ceramidase      

Alternative Name

Neutral N-acylsphingosine amidohydrolase
Neutral acylsphingosine deacylase
Slug-a-bed protein
Acylsphingosine deacylase
N-acylsphingosine amidohydrolase

Location in the cell

Lysosomal   Reliability : 0.886

CDS

ATGTTATTCGCGTGGTGCGTGCTTGCGTGCGTGACGGCGGCGGATGCGCTGCGTGTCGGAGCTGGCATAGCTGACGTCACTGGCCCACCGGCTGAAATTGCTTTTATGGGCTATGCTCAACTCGAGCAGATCGGGCATGGAATTCACCTGCGTCAGTTCTCAAGGGCCTTCGTGATAGAAGATAACAGTGGGGACACTGTTAAACGACTGGTCTTCGTGTCTGTTGATGCTGCGATGATGGGACATGGAGTTAGGAAAGAGGTAATAAGACGGCTGCAGAAGCGCTTTGGCGTAATCTACAACGAGGATAATGTGATCATCAGCGGCACCCACACTCACTCCACTCCTGGAGGATTTCTAATGGATTTCCTCTTCGATCTACCCATCCTTGGATTCGTGAAGGAGACGTACATAGCTTATGTCCTTGGAATTTATAAGAGCATAGTAATAGCTCACAGCAGGCTGACGTCGGCTCGGATAAAATACGGCGAGGCTGAACTTCTAGATGCGAATATCAACAGATCTCCGAAGTCATACTTGAACAATCCGGCAGAAGAAAGATCAAAATACAAATACGACGTGGATAAGACGTTGTCTCAAGTGAGATTCCTTGCAACGACGGGCGAGGTCATCGGCGTGATCAACTGGTTCGCCGTCCACCCGACCAGCATGAACAACACCAATAGGCTGGTGTCCTCGGACAACGTCGGATACGCTTCGCTACTCATGGAGAAGGCTCTGAATGGGAACAATACGTTACCGGGGAAAGGCGCAATAGTCTGCGCGTTCGGGTCAACGAACCTCGGCGACGTGTCCCCCAACACGCGCGGCCCGCGCTGCGAGCGGAGCGGCCGGCCGTGCGACCAGCAGGAGCAGCTCTGCGGGAAAAAGGAGCGCTGCTTCGCGTCCGGCCCGGGCAGGGACATGTTCGACAGCACTAGGATCATCGCTACCAAACTATTCGAAACTGCCATGAAAGTTCTCAGACAGCCCGGGGAAGATATAACTGGCACAGTGGCCGTGGCCCACCAATACGTCAAGATGCCAGATGAAGAAGTCCACCCATTCGATCCTGTGACAGAAACATTTAACATTAGTGTAAAAGTGAAGGGCTGTCTCCCGGCCATGGGGTATAGTTTCGCAGCAGGGACCACTGACGGGCCAGGGGCGTTTGACTTCAAACAGGGAACTAAAACATCTAACCCTCTCTGGAACGCCGTGAGGGACTTCATAGCCGAGCCGACGGAGGACGATGTACAGTGCCAAGCTCCAAAGCCCATCCTGCTGGCCACCGGAAGGGCTAAGTTCCCTTACGAGTGGCAGCCCCGGGTGGTGTCGTGCGCGGTCGCCAGGATCGGCTCGTTGTACTTGGCGGCCGTCCCCGGGGAGTTCACCACCATGTCTGGGAGGAGATTGCGGAACATCATCTCCAAGACCGCCCCACGACCCAACCGGGTCGTCATAGCCGGCCTCTCCAACATATACTCGGACTACGTGGCTACCCCTGAGGAGTACCAGGTGCAAAGGTACGAAGCAGCTTCAACCATCTACGGGCCCCACACATTGGACATCTACTTGAACAAATACCTTGAACTCACCGAGGCTCTACTCGAGAACAAAACCCCCGAGTCTGGTCCGGACCCTCCTGACTTGAGCGGCCAGCTGATCACTCTGGTGCCCCCCGTGCTGTGGGACTCCGCCCCCTGGAGGAAGGAGTTTGGAGACTGCGTGCAGCAACCGCAGTCCAGCTACAGCTACGGAGACGTCGTGGTAGCCGCTTTTGTGTCGGGACACCCACGGAACAGCGTCCGCCACGGCCGCTGGTACATGGCCGTAGAGAAACTCGAATCGGAGATCGATGACGCCTGGACTGTCATCGCGACTGATGCGGATTGGGAGACCAAATTCACCTGGCACCGTGACTCCAAAGTCTTGGGAACGAGTCACGTGGAACTCGAATGGGAGATACCCCCCGGGACTCCCCCCGGTACGTATCGGCTCCACCACTACGGGAACTACAAGTACGTTTTGGGTGGGATATACCCATACCACGGCTTCACTGATAGCTTCAAGGTCTCTTAA

Protein

MLFAWCVLACVTAADALRVGAGIADVTGPPAEIAFMGYAQLEQIGHGIHLRQFSRAFVIEDNSGDTVKRLVFVSVDAAMMGHGVRKEVIRRLQKRFGVIYNEDNVIISGTHTHSTPGGFLMDFLFDLPILGFVKETYIAYVLGIYKSIVIAHSRLTSARIKYGEAELLDANINRSPKSYLNNPAEERSKYKYDVDKTLSQVRFLATTGEVIGVINWFAVHPTSMNNTNRLVSSDNVGYASLLMEKALNGNNTLPGKGAIVCAFGSTNLGDVSPNTRGPRCERSGRPCDQQEQLCGKKERCFASGPGRDMFDSTRIIATKLFETAMKVLRQPGEDITGTVAVAHQYVKMPDEEVHPFDPVTETFNISVKVKGCLPAMGYSFAAGTTDGPGAFDFKQGTKTSNPLWNAVRDFIAEPTEDDVQCQAPKPILLATGRAKFPYEWQPRVVSCAVARIGSLYLAAVPGEFTTMSGRRLRNIISKTAPRPNRVVIAGLSNIYSDYVATPEEYQVQRYEAASTIYGPHTLDIYLNKYLELTEALLENKTPESGPDPPDLSGQLITLVPPVLWDSAPWRKEFGDCVQQPQSSYSYGDVVVAAFVSGHPRNSVRHGRWYMAVEKLESEIDDAWTVIATDADWETKFTWHRDSKVLGTSHVELEWEIPPGTPPGTYRLHHYGNYKYVLGGIYPYHGFTDSFKVS

Description

Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-7.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Regulates synaptic vesicle exocytosis and trafficking by controlling presynaptic terminal sphingolipid composition.
Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-7.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface (By similarity).

Catalytic Activity

an N-acylsphing-4-enine + H2O = a fatty acid + sphing-4-enine

Miscellaneous

Overexpression rescues retinal degeneration in arrestin and phospholipase C mutants, probably by facilitating membrane turnover and endocytosis of rhodopsin in photoreceptors.

Similarity

Belongs to the neutral ceramidase family.

Keywords

Complete proteome   Glycoprotein   Hydrolase   Lipid metabolism   Reference proteome   Secreted   Signal   Sphingolipid metabolism  

Feature

chain  Neutral ceramidase

Uniprot

H9JLR3   A0A2H1VYE1   A0A2A4K9C0   A0A1E1WP24   A0A194PI22   A0A2W1B8Z5   + More
A0A194QTJ8   A0A0L7LUS4   A0A212EIH2   A0A195DRJ6   A0A158NBE4   A0A1W4WKF1   A0A232F654   A0A195FCN6   K7ITX7   A0A026W3S6   A0A3L8DSF7   Q173S2   A0A1S4FFJ2   A0A154P9R8   A0A2K8JRS6   A0A084VN16   A0A023F618   A0A224XFS4   B0WGE6   A0A0P4VKF0   A0A182U547   A0A0C9RVC1   A0A182G996   A0A1I8PNB8   A0A1Q3FNI5   A0A1Q3FNN9   A0A182Y541   A0A1Q3FNZ9   A0A182QIH4   A0A182RL44   A0A1Q3FNM7   D6X3Y4   A0A1L8DPV5   A0A1L8EGU5   A0A1B0CQR1   A0A1L8EGN3   T1PE63   Q7QI06   A0A182PPX0   E9IBK4   A0A182W445   A0A0L0BLG6   A0A1A9ZZK7   I2CMG2   A0A067RJZ6   A0A182FIG3   W5JJX8   A0A1L8DQ15   V5GV97   A0A2M4ATB9   A0A1B0FRH6   A0A182JG20   R4N4U2   A0A034V4F0   A0A0K8USE8   A0A1A9WN33   A0A182MZX1   A0A2M4BFF7   A0A146ME47   A0A0A9W5T8   B4K8R6   A0A0M4EGS5   A0A1A9XTC7   B4LYM3   A0A182US50   A0A0A1WH00   D6X3Y5   B4JVA2   A0A0A1WKA2   B4R1M6   B4HZR3   B3P584   A4V3N7   B4PLW9   Q9VA70   A0A0R3NKD0   Q29C43   B4NGW1   W8BUB2   A0A3B0K2B9   A0A3B0JCJ4   A0A3B0K988   A0A1W4VRB3   A0A336ML87   A0A182IDP2   A0A182XNH7   A0A0A9WE73   A0A1J1J8W7   A0A1Q3FFP1   A0A1Q3FFN4   A0A1B0BUR0   B3MTB9   A0A182U2Y6   A0A182UYT8  

EC Number

3.5.1.23

Pubmed

19121390    26354079    28756777    26227816    22118469    21347285    + More
28648823    20075255    24508170    30249741    17510324    24438588    25474469    27129103    26483478    25244985    18362917    19820115    25315136    12364791    14747013    17210077    21282665    26108605    22517621    24845553    20920257    23761445    23601004    25348373    26823975    25401762    17994087    18057021    25830018    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    26109357    26109356    17550304    12153720    12637747    15356190    14769922    15632085    24495485   

EMBL

BABH01005208    BABH01005209    BABH01005210    ODYU01005218    SOQ45860.1    NWSH01000039    + More
PCG80383.1    GDQN01002261    JAT88793.1    KQ459603    KPI92967.1    KZ150341    PZC71311.1    KQ461194    KPJ06871.1    JTDY01000041    KOB79228.1    AGBW02014643    OWR41277.1    KQ980581    KYN15461.1    ADTU01011000    ADTU01011001    ADTU01011002    ADTU01011003    ADTU01011004    ADTU01011005    ADTU01011006    ADTU01011007    ADTU01011008    ADTU01011009    NNAY01000885    OXU26062.1    KQ981673    KYN38173.1    KK107453    EZA50648.1    QOIP01000004    RLU23242.1    CH477417    EAT41313.1    KQ434839    KZC07968.1    KY030989    ATU82740.1    ATLV01014673    KE524978    KFB39360.1    GBBI01002065    JAC16647.1    GFTR01007768    JAW08658.1    DS231926    EDS26943.1    GDKW01001794    JAI54801.1    GBYB01011646    JAG81413.1    JXUM01049339    KQ561600    KXJ78038.1    GFDL01005885    JAV29160.1    GFDL01005826    JAV29219.1    GFDL01005839    JAV29206.1    AXCN02002023    GFDL01005878    JAV29167.1    KQ971379    EEZ97353.1    GFDF01005592    JAV08492.1    GFDG01000986    JAV17813.1    AJWK01023885    AJWK01023886    AJWK01023887    GFDG01000985    JAV17814.1    KA646243    AFP60872.1    AAAB01008811    EAA04917.3    GL762111    EFZ22084.1    JRES01001702    KNC20803.1    JQ308540    AFJ68095.1    KK852425    KDR24151.1    ADMH02000912    ETN64687.1    GFDF01005593    JAV08491.1    GALX01004308    JAB64158.1    GGFK01010693    MBW44014.1    CCAG010022123    JX569799    AGL39308.1    GAKP01021563    JAC37389.1    GDHF01022808    GDHF01014766    GDHF01001327    JAI29506.1    JAI37548.1    JAI50987.1    GGFJ01002644    MBW51785.1    GDHC01001347    JAQ17282.1    GBHO01040465    GBRD01013004    GBRD01013003    JAG03139.1    JAG52822.1    CH933806    EDW15485.1    KRG01581.1    CP012526    ALC47628.1    CH940650    EDW68043.1    KRF83622.1    GBXI01016527    JAC97764.1    EEZ97352.2    CH916374    EDV91422.1    GBXI01015337    JAC98954.1    CM000364    EDX15014.1    CH480819    EDW53520.1    CH954182    EDV53067.1    AE014297    BT150409    AAF57052.1    AAN14232.1    AAN14233.1    AAN14234.1    AGB96488.1    AHA95713.1    CM000160    EDW99106.1    KRK04661.1    KRK04662.1    AB112076    BT004471    CM000070    KRS99567.1    CH964272    EDW84458.1    GAMC01013846    GAMC01013845    JAB92709.1    OUUW01000005    SPP80099.1    SPP80097.1    SPP80098.1    UFQT01001467    SSX30700.1    APCN01004753    GBHO01040464    GBRD01013007    GBRD01013005    JAG03140.1    JAG52819.1    CVRI01000074    CRL07958.1    GFDL01008667    JAV26378.1    GFDL01008699    JAV26346.1    JXJN01020882    JXJN01020883    CH902623    EDV30509.1    KPU72902.1   

Proteomes

UP000005204    UP000218220    UP000053268    UP000053240    UP000037510    UP000007151    + More
UP000078492    UP000005205    UP000192223    UP000215335    UP000078541    UP000002358    UP000053097    UP000279307    UP000008820    UP000076502    UP000030765    UP000002320    UP000075902    UP000069940    UP000249989    UP000095300    UP000076408    UP000075886    UP000075900    UP000007266    UP000092461    UP000095301    UP000007062    UP000075885    UP000075920    UP000037069    UP000092445    UP000027135    UP000069272    UP000000673    UP000092444    UP000075880    UP000091820    UP000075884    UP000009192    UP000092553    UP000092443    UP000008792    UP000075903    UP000001070    UP000000304    UP000001292    UP000008711    UP000000803    UP000002282    UP000001819    UP000007798    UP000268350    UP000192221    UP000075840    UP000076407    UP000183832    UP000092460    UP000007801   

PRIDE

H9JLR3    A4V3N7    Q9VA70   

Pfam

PF17048   Ceramidse_alk_C        + More
PF04734   Ceramidase_alk

Interpro

IPR038445   NCDase_C_sf        + More
IPR006823   Ceramidase_alk       
IPR031331   NEUT/ALK_ceramidase_C       
IPR031329   NEUT/ALK_ceramidase_N       
IPR000953   Chromo/chromo_shadow_dom       
IPR016197   Chromo-like_dom_sf       

SUPFAM

SSF54160   SSF54160       

Gene 3D

2.60.40.2300    

CDD

cd00024  CHROMO

ProteinModelPortal

H9JLR3   A0A2H1VYE1   A0A2A4K9C0   A0A1E1WP24   A0A194PI22   A0A2W1B8Z5   + More
A0A194QTJ8   A0A0L7LUS4   A0A212EIH2   A0A195DRJ6   A0A158NBE4   A0A1W4WKF1   A0A232F654   A0A195FCN6   K7ITX7   A0A026W3S6   A0A3L8DSF7   Q173S2   A0A1S4FFJ2   A0A154P9R8   A0A2K8JRS6   A0A084VN16   A0A023F618   A0A224XFS4   B0WGE6   A0A0P4VKF0   A0A182U547   A0A0C9RVC1   A0A182G996   A0A1I8PNB8   A0A1Q3FNI5   A0A1Q3FNN9   A0A182Y541   A0A1Q3FNZ9   A0A182QIH4   A0A182RL44   A0A1Q3FNM7   D6X3Y4   A0A1L8DPV5   A0A1L8EGU5   A0A1B0CQR1   A0A1L8EGN3   T1PE63   Q7QI06   A0A182PPX0   E9IBK4   A0A182W445   A0A0L0BLG6   A0A1A9ZZK7   I2CMG2   A0A067RJZ6   A0A182FIG3   W5JJX8   A0A1L8DQ15   V5GV97   A0A2M4ATB9   A0A1B0FRH6   A0A182JG20   R4N4U2   A0A034V4F0   A0A0K8USE8   A0A1A9WN33   A0A182MZX1   A0A2M4BFF7   A0A146ME47   A0A0A9W5T8   B4K8R6   A0A0M4EGS5   A0A1A9XTC7   B4LYM3   A0A182US50   A0A0A1WH00   D6X3Y5   B4JVA2   A0A0A1WKA2   B4R1M6   B4HZR3   B3P584   A4V3N7   B4PLW9   Q9VA70   A0A0R3NKD0   Q29C43   B4NGW1   W8BUB2   A0A3B0K2B9   A0A3B0JCJ4   A0A3B0K988   A0A1W4VRB3   A0A336ML87   A0A182IDP2   A0A182XNH7   A0A0A9WE73   A0A1J1J8W7   A0A1Q3FFP1   A0A1Q3FFN4   A0A1B0BUR0   B3MTB9   A0A182U2Y6   A0A182UYT8  

PDB

4WGK     E-value=7.99273e-159,     Score=1440

KEGG

101744519    K12349 neutral ceramidase [EC:3.5.1.23] | (RefSeq) neutral ceramidase

PATHWAY

00600    Sphingolipid metabolism - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0017040   GO:0046514   GO:0046512   GO:0005576   GO:0042759   GO:0016021   GO:0031629   GO:0005737   GO:0098793   GO:0045494   GO:0035187   GO:0102121   GO:0007268   GO:0016079   GO:0006665  

PANTHER

PTHR12670  

Subcellular location

Secreted  

SignalP

Position:   1 - 16,         Likelihood:  0.966831

 

 

Length:

693

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

4.98984999999999

Exp number, first 60 AAs:

0.5606

Total prob of N-in:

0.03359

outside

1  -  693

 

 

Pi

208.130216

Theta

16.09512

Tajima's D

0.966313

CLR

1.035918

CSRT

0.658317084145793

Interpretation

Uncertain