Gene

KWMTBOMO07015  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA010467

Annotation

PREDICTED:_bifunctional_ATP-dependent_dihydroxyacetone_kinase/FAD-AMP_lyase_(cyclizing)-like?_partial_[Bombyx_mori]

Location

Bomo_Chr12(-):1863526-1877123      View in EpiBrowser

Full name

Triokinase/FMN cyclase      

Alternative Name

Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing)

Location in the cell

Cytoplasmic   Reliability : 2.362

CDS

ATGCTTGGCTTTCAGGATGACAGTAAGAGAGTGGCCATCCTTGGTGGTGGAGGATCGGGACACGAACCCTTTGCATCAGGATTCATAGGTTCGGGAATGCTCGATGGCGCAGTGGCTGGTGGGGTTTTCGCTTCACCACCTACTGGACATGTATTGTACGGGATAGCGCAACTACACAAATATAATTCAGGGGGAGTTCTTGTTATCATTGGTAATTACACAGGCGATCGGCTAAACTTCGGCAAGGCCATCGAGAAAGCTAAAATAGCAGGAATTAAGGTGGAAGGTCTTATCGTGGGAGAGGATGTGGCTTCAAGCAAGAACAAAACAGGCGGGCGCAGTATGGTCGGAGAAGTACTATTTTATAAGCTATGTGGAGCAATGGCCTTAAAAGGTTACGACCTTGAGACGATACGAAAGACTGCAGTTGAAGCTAACAAAAACATGGCCACCCTAGGCGTGTGCCTCAGCGCTTGCTCTCTGCCAGGTCAGCCGCCGCTGTTCGAGATATCACCTGACGAGATGGAACTCGGCGCTGGTGTACACGGCGAAGCTGGTATAGCGAAAGTCAAAATGGGTACCGCCAAGGAAATCGTCAAGATGCTTCTAGCTATAATTGTTGGTCATTTAAAATTATCACGGGGAGATCGGGTAGCGGTCACAGTGGACAACTTAGGTGGTACTTCGTTCTTGGAAATGAATATCATCAGCGCTGAAACTAAGGATTACTTAGAAAACCACGATATCACGGTAGAACGTGTATACGCGGGGCACTTAAAGACCTCACTAGAGATGCACGGATTTCAAATATGTCTTCTGCTTCTAAACAAGGAGCATGGCGATCTCTTCCTGGAGTTATTGGACGCACCCACTTCAGCTGCAGCTTGGACTGGTGGTGAAATATCCGTGAGGAGTGACGGAGGACATGTCGACGATGAGAGCTTGTTGAAAGGTGACGTTAGGAAGGCGGCTTCGGGCCCGTCTTTGTCAGCGAAACAGCAAGATCTTCTCCGCGAGAGTTTGACCTCCGCTGCAGAAGTACTGATTAAGAGCGAGGCTTTACTGAATAAACTAGACTCAGGGTGCGGAGATGGCGACTGCGGGAATACGCTTAAAAAGTTTGGACACGCCATCTTAGCATATCTGAAGACAGCTTCGTTAGAATATGTGTCCAACGTACTCTGGGACTTGTCAGAAATCGCCGAGACCGACATGGGCGGGACCTCGGGTGGAATCTACAGCTTGGGGCTTTCGGCTGCATCACAGGCATTTTCAATAGCTACGTCCAACTCTGTCCCAACATGGCTGTCGGCGTTGGAGAGCGCAATACAAGCTATTTCAAAATACGGCGGTGCTGAGCCCGGCGATAGGACAATGTTGGACACTTTGCATGCAGTAGCTGAAACGTTGAAGAAGTGTATCGAAGAAGAACTGTTAGCGGTGCTGAAGAAGGCTGCAGAAGCTGCCGATCACGGCGCCAAGGCCACAGCGAGTATGGTTGCAAGAGCTGGTAGAGCATCGTACGTGGCTAGCGGTTATACGCAGCAAGAAGACGCTGGGGCAAGAGCAGCAGCTCTTTGGCTACAAGCTATACTGTGTTCTATCGCTAGTAAAATATGA

Protein

MLGFQDDSKRVAILGGGGSGHEPFASGFIGSGMLDGAVAGGVFASPPTGHVLYGIAQLHKYNSGGVLVIIGNYTGDRLNFGKAIEKAKIAGIKVEGLIVGEDVASSKNKTGGRSMVGEVLFYKLCGAMALKGYDLETIRKTAVEANKNMATLGVCLSACSLPGQPPLFEISPDEMELGAGVHGEAGIAKVKMGTAKEIVKMLLAIIVGHLKLSRGDRVAVTVDNLGGTSFLEMNIISAETKDYLENHDITVERVYAGHLKTSLEMHGFQICLLLLNKEHGDLFLELLDAPTSAAAWTGGEISVRSDGGHVDDESLLKGDVRKAASGPSLSAKQQDLLRESLTSAAEVLIKSEALLNKLDSGCGDGDCGNTLKKFGHAILAYLKTASLEYVSNVLWDLSEIAETDMGGTSGGIYSLGLSAASQAFSIATSNSVPTWLSALESAIQAISKYGGAEPGDRTMLDTLHAVAETLKKCIEEELLAVLKKAAEAADHGAKATASMVARAGRASYVASGYTQQEDAGARAAALWLQAILCSIASKI

Description

Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate. Represses IFIH1-mediated cellular antiviral response (PubMed:17600090).

Catalytic Activity

ATP + dihydroxyacetone = ADP + dihydroxyacetone phosphate + H(+)
ATP + D-glyceraldehyde = ADP + D-glyceraldehyde 3-phosphate + H(+)
FAD = AMP + H(+) + riboflavin cyclic-4',5'-phosphate

Cofactor

Mg(2+)

Biophysicochemical Properties

0.5 uM for dihydroxyacetone
11 uM for glyceraldehyde
1.55 uM for dihydroxyacetone
43.2 uM for ATP
18.1 uM for glyceraldehyde
7 uM for FAD
12 uM for ADP-glucose
317 uM for UDP-glucose
263 uM for UDP-galactose

Subunit

Homodimer (By similarity). Interacts with IFIH1 (via the CARD domains), the interaction is inhibited by viral infection (PubMed:17600090).

Similarity

Belongs to the dihydroxyacetone kinase (DAK) family.

Keywords

Alternative splicing   ATP-binding   Cobalt   Complete proteome   FAD   Flavoprotein   Kinase   Lyase   Magnesium   Manganese   Metal-binding   Multifunctional enzyme   Nucleotide-binding   Phosphoprotein   Polymorphism   Reference proteome   Transferase  

Feature

chain  Triokinase/FMN cyclase
splice variant  In isoform 2.
sequence variant  In dbSNP:rs2260655.

Uniprot

A0A2A4K9H2   A0A2W1B4U0   A0A194QMW4   A0A194PPA9   A0A2H1VBP3   A0A0L7L3Z9   + More
A0A212F8X5   A0A154PGQ1   A0A2R2MMQ2   C3ZQZ9   A0A2R2MMP3   A0A1S3IBP5   A0A151INV0   A0A151JT26   F4W7H7   A0A067RGB2   A0A2J7RBU9   A0A026WU04   K1PIX8   A0A1B6D265   A0A2J7RBX7   A0A232ERT0   E2A2U3   A0A088AEP8   A0A2A3EBN0   A0A151X8J8   E9IX13   K7ITH9   E2B9B1   K1PR20   A0A0C9RHJ1   H2XLN7   A0A1W2WHJ9   F1Q8Q6   A0A0H5SDC7   A0A3M6THH3   A0A0B2VND3   A0A1A8NNV3   A0A1A8S4J7   A0A1A8UW57   L8Y5J3   A0A1A8F8N5   A0A3B4CTM6   I3MA42   A0A1A8IZJ0   A0A195AT93   W5MTB7   G1SFW1   A0A158NPY8   C3XZK9   H3BDK7   A0A1S3G900   A0A2B4T0J0   A0A0N4TLW6   Q08CB4   A0A3N0YI63   A0A3Q1AHF4   A0A024R529   W5KUR9   A0A2K5PUJ5   H2ND85   G3QN84   G2HF99   A0A140VJH7   Q3LXA3   F7GX62   H9EV13   A0A2K5KEP8   A0A238C3Z8   A0A2K6KTG4   A0A2K6S7W7   W5ULD7   A0A2D0RAI8   A0A2K6EG49   G1QPV1   D2HUE6   A0A2K6S7X4   F6TNX4   A0A2K6EG62   H3BDK8   A0A096MPA6   A0A1A7XB45   A0A0D9R252   G1MDS9   A0A1A7YB51   A0A2K6BKC7   A0A2K5V408   I0FMH5   G7PPY4   A0A2K5P3L9   A0A2D0RCG5   U3KBU5   A0A2D0RBR1   A0A2K6P8B6   A0A2K5EX23   A0A2U3XGC2   A0A212DGA4   A0A3Q7PM29   T1IL93  

Pubmed

28756777    26354079    26227816    22118469    26383154    18563158    + More
21719571    24845553    24508170    22992520    28648823    20798317    21282665    20075255    12481130    15114417    23594743    17885136    30382153    23385571    21993624    21347285    9215903    11181995    25329095    22398555    16136131    21484476    16289032    14702039    16554811    15489334    4688871    17600090    21269460    24569995    24275569    25319552    23127152    20010809    19892987    17431167    22002653    25362486    29294181   

EMBL

NWSH01000039    PCG80380.1    KZ150341    PZC71309.1    KQ461194    KPJ06868.1    + More
KQ459603    KPI92970.1    ODYU01001690    SOQ38259.1    JTDY01003081    KOB70182.1    AGBW02009660    OWR50180.1    KQ434893    KZC10644.1    GG666663    EEN45040.1    KQ976914    KYN07115.1    KQ982014    KYN30670.1    GL887844    EGI69850.1    KK852482    KDR22916.1    NEVH01005889    PNF38316.1    KK107105    EZA59458.1    JH817601    EKC18869.1    GEDC01017507    JAS19791.1    PNF38318.1    NNAY01002582    OXU20996.1    GL436214    EFN72250.1    KZ288301    PBC28874.1    KQ982409    KYQ56693.1    GL766616    EFZ14941.1    AAZX01003331    GL446479    EFN87717.1    EKC18870.1    GBYB01006396    JAG76163.1    EAAA01001926    BX957278    LN855247    CRZ21959.1    RCHS01003571    RMX40845.1    JPKZ01001291    KHN82859.1    HAEG01004056    SBR70671.1    HAEH01021967    SBS13171.1    HAEJ01011369    SBS51826.1    KB368149    ELV10250.1    HAEB01008599    HAEC01011888    SBQ55126.1    AGTP01066789    HAED01016276    SBR02721.1    KQ976745    KYM75443.1    AHAT01015510    AHAT01015511    AHAT01015512    ADTU01022711    ADTU01022712    GG666475    EEN66530.1    AFYH01010944    AFYH01010945    AFYH01010946    AFYH01010947    LSMT01000001    PFX34853.1    UZAD01013153    VDN90550.1    BC124306    AAI24307.1    RJVU01042551    ROL45561.1    CH471076    EAW73940.1    ABGA01076013    NDHI03003558    PNJ22124.1    CABD030079452    AACZ04057297    AK305413    GABC01002456    GABF01000624    GABD01010396    GABE01006961    NBAG03000297    BAK62407.1    JAA08882.1    JAA21521.1    JAA22704.1    JAA37778.1    PNI45179.1    HM005364    AEE60964.1    DQ138290    DQ344550    AK023915    AK223580    AP003108    BC001341    JU322466    AFE66222.1    KZ269978    OZC12192.1    JT419119    AHH42964.1    ADFV01078994    GL193398    EFB20941.1    AHZZ02006390    HADW01013700    SBP15100.1    AQIB01079262    ACTA01020162    HADX01005132    SBP27364.1    AQIA01020060    JSUE03012184    JV045580    JV636090    AFI35651.1    AFJ71430.1    CM001289    EHH56174.1    AGTO01011732    MKHE01000002    OWK17273.1    JH430755   

Proteomes

UP000218220    UP000053240    UP000053268    UP000037510    UP000007151    UP000076502    + More
UP000085678    UP000001554    UP000078542    UP000078541    UP000007755    UP000027135    UP000235965    UP000053097    UP000005408    UP000215335    UP000000311    UP000005203    UP000242457    UP000075809    UP000002358    UP000008237    UP000008144    UP000000437    UP000006672    UP000275408    UP000031036    UP000011518    UP000261440    UP000005215    UP000078540    UP000018468    UP000001811    UP000005205    UP000008672    UP000081671    UP000225706    UP000038020    UP000278627    UP000257160    UP000018467    UP000233040    UP000001595    UP000001519    UP000002277    UP000005640    UP000008225    UP000233080    UP000233180    UP000233220    UP000221080    UP000233160    UP000001073    UP000002281    UP000028761    UP000029965    UP000008912    UP000233120    UP000233100    UP000006718    UP000009130    UP000233060    UP000016665    UP000233200    UP000233020    UP000245341    UP000286641   

PRIDE

A0A088AEP8    A0A140VJH7    Q3LXA3   

Pfam

PF02734   Dak2        + More
PF02733   Dak1

Interpro

IPR004007   DhaL_dom        + More
IPR004006   DhaK_dom       
IPR036117   DhaL_dom_sf       
IPR012734   DhaK_ATP       

SUPFAM

SSF101473   SSF101473       

Gene 3D

1.25.40.340    

ProteinModelPortal

A0A2A4K9H2   A0A2W1B4U0   A0A194QMW4   A0A194PPA9   A0A2H1VBP3   A0A0L7L3Z9   + More
A0A212F8X5   A0A154PGQ1   A0A2R2MMQ2   C3ZQZ9   A0A2R2MMP3   A0A1S3IBP5   A0A151INV0   A0A151JT26   F4W7H7   A0A067RGB2   A0A2J7RBU9   A0A026WU04   K1PIX8   A0A1B6D265   A0A2J7RBX7   A0A232ERT0   E2A2U3   A0A088AEP8   A0A2A3EBN0   A0A151X8J8   E9IX13   K7ITH9   E2B9B1   K1PR20   A0A0C9RHJ1   H2XLN7   A0A1W2WHJ9   F1Q8Q6   A0A0H5SDC7   A0A3M6THH3   A0A0B2VND3   A0A1A8NNV3   A0A1A8S4J7   A0A1A8UW57   L8Y5J3   A0A1A8F8N5   A0A3B4CTM6   I3MA42   A0A1A8IZJ0   A0A195AT93   W5MTB7   G1SFW1   A0A158NPY8   C3XZK9   H3BDK7   A0A1S3G900   A0A2B4T0J0   A0A0N4TLW6   Q08CB4   A0A3N0YI63   A0A3Q1AHF4   A0A024R529   W5KUR9   A0A2K5PUJ5   H2ND85   G3QN84   G2HF99   A0A140VJH7   Q3LXA3   F7GX62   H9EV13   A0A2K5KEP8   A0A238C3Z8   A0A2K6KTG4   A0A2K6S7W7   W5ULD7   A0A2D0RAI8   A0A2K6EG49   G1QPV1   D2HUE6   A0A2K6S7X4   F6TNX4   A0A2K6EG62   H3BDK8   A0A096MPA6   A0A1A7XB45   A0A0D9R252   G1MDS9   A0A1A7YB51   A0A2K6BKC7   A0A2K5V408   I0FMH5   G7PPY4   A0A2K5P3L9   A0A2D0RCG5   U3KBU5   A0A2D0RBR1   A0A2K6P8B6   A0A2K5EX23   A0A2U3XGC2   A0A212DGA4   A0A3Q7PM29   T1IL93  

PDB

1UN8     E-value=1.1933e-47,     Score=480

KEGG

101738177    K00863 triose/dihydroxyacetone kinase / FAD-AMP lyase (cyclizing) [EC:2.7.1.28 2.7.1.29 4.6.1.15] |

PATHWAY

00051    Fructose and mannose metabolism - Bombyx mori (domestic silkworm)
00561    Glycerolipid metabolism - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)
01200    Carbon metabolism - Bombyx mori (domestic silkworm)

GO

GO:0006071   GO:0004371   GO:0016829   GO:0005524   GO:0005829   GO:0019563   GO:0050354   GO:0039534   GO:0046835   GO:0034012   GO:0016301   GO:0016021   GO:0045087   GO:0005634   GO:0046872   GO:0061624   GO:0070062   GO:0044262   GO:0045088   GO:0006468   GO:0007018   GO:0030286   GO:0005515   GO:0004319   GO:0004320   GO:0016295   GO:0016296  

Length:

539

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.29933

Exp number, first 60 AAs:

0.23288

Total prob of N-in:

0.00942

outside

1  -  539

 

 

Pi

201.494354

Theta

149.017681

Tajima's D

0.818716

CLR

0.174285

CSRT

0.599570021498925

Interpretation

Uncertain