Gene

KWMTBOMO06907  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA004646

Annotation

PREDICTED:_mitotic_checkpoint_protein_BUB3_[Papilio_machaon]

Location

Bomo_Chr11(-):19624686-19629575      View in EpiBrowser

Full name

Mitotic checkpoint protein BUB3      

Location in the cell

Nuclear   Reliability : 1.564

CDS

ATGTCAGCACAGACTAAGAAAATTGCTTCCACAATGACTGTCACACGGGTGGCGGAGTCGCGCACCGAGTTCAAATTAAAAAGCTTACCCGAAGACGCTATTTCGAGCGTTAAATTTGCACCGAAATCTAACCAGTACTTGCTGGTATCTTCATGGGACTGTTCTGTGAGGCTTTATGATGTGACCGCGAATATAGAAAGACATAAATACAATCATGAACTGCCGGTTCTTGATGTTTGTTTTCGGGATGCTGTTCATTCATATAGTGGTGGACTAGATCAGACTCTCAAAATGTATGATTTGAATGCTAGCACGGAGACTATCCTCGGAGAGCACAAAGGAGCTATACGCTGTGTAGAGTTCGCATCTGAACTTAATGCAGTACTCACAGGAAGCTGGGATGGAACAGTCAAAATGTGGGATAGCAGAGTTCCAAACTGTGTCGGCACTTACAATCAGGGAAATGAACGGGTATACACAATGAGCATAGTTGGCGAAAAGTTCGTAGTTGGTACATCGGGACGTAAAATATTTGTATGGGACGTTCGTAACATGGGGCATGTAAATCAAAGGAGGGAATCCTCACTCAAATATCAAACACGATGCATACGAGTGTTTCCCAATAAGCAGGGCTACGTACTCAGCTCAATAGAGGGACGTGTGGCGGTGGAGTATTTGGACAGTAATCCTGAGGTGCAGAAGAAGAAATATGCTTTCAAATGCCATCGGATTAAAGAAGGAGGTCTAGAAAAGATATATCCAGTGAACGCGATCAGCTTCCACTCCGTGTACAACACGTTCGCGACGGGCGGCTCGGACGGCTACGTGAACATCTGGGACGGCTTCAACAAGAAGCGGCTGTGCCAGTTCCACCGCTACAACACCGCCGTCTCCGCGCTCGCCTTCTCGCACGACGGCTCCGCGCTCGCCATCGCCTGCTCGCAGCTCGACGACTCGCAGATCGAAGCCTCCAAGCCCGAAGACACCATCTACATACGATACGTCACGGACCAGGAAACGAAGCCTAAATGA

Protein

MSAQTKKIASTMTVTRVAESRTEFKLKSLPEDAISSVKFAPKSNQYLLVSSWDCSVRLYDVTANIERHKYNHELPVLDVCFRDAVHSYSGGLDQTLKMYDLNASTETILGEHKGAIRCVEFASELNAVLTGSWDGTVKMWDSRVPNCVGTYNQGNERVYTMSIVGEKFVVGTSGRKIFVWDVRNMGHVNQRRESSLKYQTRCIRVFPNKQGYVLSSIEGRVAVEYLDSNPEVQKKKYAFKCHRIKEGGLEKIYPVNAISFHSVYNTFATGGSDGYVNIWDGFNKKRLCQFHRYNTAVSALAFSHDGSALAIACSQLDDSQIEASKPEDTIYIRYVTDQETKPK

Description

Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.

Subunit

Interacts with BUB1 and BUBR1. The BUB1/BUB3 complex interacts with MAD1L1. Interacts with ZNF207/BuGZ; leading to promote stability and kinetochore loading of BUB3.

Similarity

Belongs to the WD repeat BUB3 family.

Keywords

Acetylation   ADP-ribosylation   Alternative splicing   Cell cycle   Cell division   Centromere   Chromosome   Chromosome partition   Complete proteome   Isopeptide bond   Kinetochore   Meiosis   Mitosis   Nucleus   Phosphoprotein   Reference proteome   Repeat   Ubl conjugation   WD repeat  

Feature

chain  Mitotic checkpoint protein BUB3
splice variant  In isoform 2.

Uniprot

H9J556   A0A194QR56   A0A2H1V339   S4P5H9   A0A194Q9S5   A0A2W1C2C1   + More
A0A2A4K7N2   A0A3S2NQT9   A0A212FLV0   A0A0L7L480   A0A2J7PN74   A0A067RB95   A0A1B6EGI1   A0A1B6FJ92   A0A1B6HME9   A0A1B6KU11   A0A1B6MF64   D6X4M0   A0A1W4W2W7   J9K808   A0A1Y1K2K2   A0A2T7P896   A0A1S3IJW8   A0A0A9WLL1   C3Y5S4   A0A0B6Y9E7   V4B165   R7USZ3   A0A195CU12   A0A0N7ZCD8   E2AWS0   A0A0L7RK02   A0A154NYT1   A0A195BB82   E2BFL7   A0A151J4R0   F4WDL5   A0A151JV88   A0A158NIM9   A0A2C9JQ13   A0A0J7L279   A0A026WZW0   A0A310SEH1   A0A151WQ25   A0A3R7SJK4   C0IRC6   A0A2A3EIJ3   A0A088AQL5   A0A232F546   K7IMF7   A0A1S3DC81   A0A3Q0J6B9   A0A293M4M7   A0A210PP70   A0A0L8FLL0   A0A2P2I2J4   K1PHV7   A0A3S1C938   J3JUZ6   S4RSZ5   T1IIQ6   A0A087UDV1   W5MNG7   W4Y561   R0LED4   V9KPJ9   W5K9X2   A0A1S2ZI75   K7FM27   A0A2I0MEU7   A0A1V4KJU8   A0A2I0MES4   U3J5P9   A0A3Q0GU34   A0A3B4BDS9   A0A3Q0GPJ4   A0A218UT65   U3JQ70   A0A3S2P801   H2M0R6   A0A3P9IXW3   G3W9J5   G3W9J6   A0A1L8FEC6   G1NGF8   G3W9J7   O43684-2   A0A1U7R8K2   A0A286XDC6   Q4FZS2   A0A384BM44   K9IJA3   A0A341BXJ2   F7B951   A0A3Q2HZV6   A0A2U4BRA8   A0A340WWE3   A0A2I3NAM9   A0A2K5EN33   A0A2Y9SFT9  

Pubmed

19121390    26354079    23622113    28756777    22118469    26227816    + More
24845553    18362917    19820115    28004739    25401762    26823975    18563158    23254933    20798317    21719571    21347285    15562597    24508170    30249741    28648823    20075255    28812685    22992520    22516182    23537049    24402279    25329095    17381049    23371554    23749191    25463417    17554307    21709235    27762356    20838655    10198256    9660858    14702039    15164054    15489334    15525512    18199686    18669648    19608861    21269460    23186163    24462186    24462187    21993624    15057822    15632090    24813606    19892987   

EMBL

BABH01040711    KQ461185    KPJ07455.1    ODYU01000452    SOQ35260.1    GAIX01005539    + More
JAA87021.1    KQ459252    KPJ02164.1    KZ149906    PZC78273.1    NWSH01000092    PCG79662.1    RSAL01000138    RVE46176.1    AGBW02007696    OWR54724.1    JTDY01003110    KOB70131.1    NEVH01023957    PNF17793.1    KK852811    KDR15933.1    GEDC01003709    GEDC01000297    JAS33589.1    JAS37001.1    GECZ01019522    JAS50247.1    GECU01031872    JAS75834.1    GEBQ01025045    JAT14932.1    GEBQ01005379    JAT34598.1    KQ971380    EEZ97704.1    ABLF02028424    GEZM01098963    JAV53606.1    PZQS01000005    PVD29616.1    GBHO01036206    GBRD01008691    GDHC01016182    JAG07398.1    JAG57130.1    JAQ02447.1    GG666487    EEN64321.1    HACG01005566    CEK52431.1    KB204089    ESO81944.1    AMQN01006420    AMQN01006421    AMQN01006422    KB298315    ELU09330.1    KQ977306    KYN03624.1    GDRN01068652    JAI64168.1    GL443424    EFN62106.1    KQ414578    KOC71195.1    KQ434777    KZC04234.1    KQ976529    KYM81811.1    GL448037    EFN85539.1    KQ980102    KYN17747.1    GL888087    EGI67795.1    KQ981702    KYN37459.1    ADTU01017000    LBMM01001164    KMQ96796.1    KK107072    QOIP01000007    EZA60659.1    RLU20878.1    KQ762400    OAD55898.1    KQ982850    KYQ49966.1    QCYY01003397    ROT63696.1    EU492542    ACD13595.1    KZ288253    PBC31006.1    NNAY01000987    OXU25600.1    GFWV01009206    MAA33935.1    NEDP02005569    OWF38295.1    KQ429209    KOF65543.1    IACF01002573    LAB68221.1    JH816779    EKC21138.1    RQTK01000143    RUS86307.1    BT127061    KB632379    AEE62023.1    ERL94090.1    JH430212    KK119382    KFM75540.1    AHAT01004030    AAGJ04038112    KB743453    EOA98647.1    JW867677    AFP00195.1    AGCU01172420    AGCU01172421    AKCR02000017    PKK28182.1    LSYS01003057    OPJ84097.1    PKK28181.1    ADON01095586    MUZQ01000141    OWK56963.1    AGTO01011741    CM012455    RVE59742.1    AEFK01004272    AEFK01004273    CM004479    OCT69916.1    AF047472    AF047473    AF053304    AF081496    AK312546    AC012391    CH471066    BC005138    BC022438    AAKN02002977    AABR07005873    BC099199    CH473953    AAH99199.1    EDM11706.1    GABZ01006998    JAA46527.1    AHZZ02036186   

Proteomes

UP000005204    UP000053240    UP000053268    UP000218220    UP000283053    UP000007151    + More
UP000037510    UP000235965    UP000027135    UP000007266    UP000192223    UP000007819    UP000245119    UP000085678    UP000001554    UP000030746    UP000014760    UP000078542    UP000000311    UP000053825    UP000076502    UP000078540    UP000008237    UP000078492    UP000007755    UP000078541    UP000005205    UP000076420    UP000036403    UP000053097    UP000279307    UP000075809    UP000283509    UP000242457    UP000005203    UP000215335    UP000002358    UP000079169    UP000242188    UP000053454    UP000005408    UP000271974    UP000030742    UP000245300    UP000054359    UP000018468    UP000007110    UP000018467    UP000079721    UP000007267    UP000053872    UP000190648    UP000016666    UP000189705    UP000261520    UP000197619    UP000016665    UP000001038    UP000265180    UP000265200    UP000007648    UP000186698    UP000001645    UP000005640    UP000189706    UP000005447    UP000002494    UP000261680    UP000291021    UP000252040    UP000002281    UP000245320    UP000265300    UP000028761    UP000233020    UP000248484   

PRIDE

H9J556    O43684    F7B951   

Pfam

PF00400   WD40

Interpro

IPR001680   WD40_repeat        + More
IPR017986   WD40_repeat_dom       
IPR015943   WD40/YVTN_repeat-like_dom_sf       
IPR036322   WD40_repeat_dom_sf       
IPR019775   WD40_repeat_CS       
IPR020472   G-protein_beta_WD-40_rep       

SUPFAM

SSF50978   SSF50978       

Gene 3D

2.130.10.10    

ProteinModelPortal

H9J556   A0A194QR56   A0A2H1V339   S4P5H9   A0A194Q9S5   A0A2W1C2C1   + More
A0A2A4K7N2   A0A3S2NQT9   A0A212FLV0   A0A0L7L480   A0A2J7PN74   A0A067RB95   A0A1B6EGI1   A0A1B6FJ92   A0A1B6HME9   A0A1B6KU11   A0A1B6MF64   D6X4M0   A0A1W4W2W7   J9K808   A0A1Y1K2K2   A0A2T7P896   A0A1S3IJW8   A0A0A9WLL1   C3Y5S4   A0A0B6Y9E7   V4B165   R7USZ3   A0A195CU12   A0A0N7ZCD8   E2AWS0   A0A0L7RK02   A0A154NYT1   A0A195BB82   E2BFL7   A0A151J4R0   F4WDL5   A0A151JV88   A0A158NIM9   A0A2C9JQ13   A0A0J7L279   A0A026WZW0   A0A310SEH1   A0A151WQ25   A0A3R7SJK4   C0IRC6   A0A2A3EIJ3   A0A088AQL5   A0A232F546   K7IMF7   A0A1S3DC81   A0A3Q0J6B9   A0A293M4M7   A0A210PP70   A0A0L8FLL0   A0A2P2I2J4   K1PHV7   A0A3S1C938   J3JUZ6   S4RSZ5   T1IIQ6   A0A087UDV1   W5MNG7   W4Y561   R0LED4   V9KPJ9   W5K9X2   A0A1S2ZI75   K7FM27   A0A2I0MEU7   A0A1V4KJU8   A0A2I0MES4   U3J5P9   A0A3Q0GU34   A0A3B4BDS9   A0A3Q0GPJ4   A0A218UT65   U3JQ70   A0A3S2P801   H2M0R6   A0A3P9IXW3   G3W9J5   G3W9J6   A0A1L8FEC6   G1NGF8   G3W9J7   O43684-2   A0A1U7R8K2   A0A286XDC6   Q4FZS2   A0A384BM44   K9IJA3   A0A341BXJ2   F7B951   A0A3Q2HZV6   A0A2U4BRA8   A0A340WWE3   A0A2I3NAM9   A0A2K5EN33   A0A2Y9SFT9  

PDB

4OWR     E-value=1.77641e-45,     Score=459

KEGG

101738254    K02180 cell cycle arrest protein BUB3 | (RefSeq) mitotic checkpoint protein BUB3

GO

GO:0043130   GO:0000776   GO:0007094   GO:0033597   GO:1990298   GO:0000070   GO:0008608   GO:0005654   GO:0034501   GO:0051983   GO:0005829   GO:0051321   GO:0000777   GO:1901990   GO:0006511   GO:0051301   GO:0031145   GO:0005515   GO:0008270   GO:0003700   GO:0005634   GO:0043565   GO:0043401   GO:0006418   GO:0016020   GO:0006260  

Subcellular location

Nucleus   Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition.   With evidence from 4 publications.
Chromosome   Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition.   With evidence from 4 publications.
Centromere   Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition.   With evidence from 4 publications.
Kinetochore   Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition.   With evidence from 4 publications.

Length:

343

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.05587

Exp number, first 60 AAs:

0.00014

Total prob of N-in:

0.06641

outside

1  -  343

 

 

Pi

16.427683

Theta

17.653741

Tajima's D

-0.316306

CLR

12.487709

CSRT

0.295285235738213

Interpretation

Uncertain