Gene

KWMTBOMO06817

Pre Gene Modal

BGIBMGA012050

Annotation

PREDICTED:_uncharacterized_protein_LOC101741648_isoform_X1_[Bombyx_mori]

Location

Bomo_Chr11(+):16026922-16027852      View in EpiBrowser

Full name

Mitochondrial cardiolipin hydrolase      

Alternative Name

Choline phosphatase 6
Mitochondrial phospholipase
Phosphatidylcholine-hydrolyzing phospholipase D6
Phospholipase D6
Protein zucchini homolog

Location in the cell

Cytoplasmic   Reliability : 2.206

CDS

ATGCCTGGGATACACAATGTAGCAATACAAGGACGCTTAGTTGAAATGCTGAAAAAGAAAAATATCAAGATTTCAATTGTAATAGATCAGTCCGGGTGCAACGAACCAAATGATGTCTTCATAAAGGAACTTTTAGATGCTGGTGCAGTAATCAAGTACATTAATACAGAACCGTATACAATGCAACATAAATTCTGTTTGATCGACGACAAAGTTTTAATGACAGGTACACTAAACTGGGGAAACGATCGATCGTCAGATCACTGGAACTACGTCTACATTACAAGCAAGCCAAAGCTAGTTGAACCAGTCAGCAAAGAGTTCAAATATATGTGGCTCAGTTCAAAAGATTTGAACTATTCCCAGTTTGATAGCAAACCTATCAACGAAGATGAGTGTTGTAACACAGAAGTCGTTAATCTTACAGAAAACTATAAATCAGTCCCGTTTGGAAATAAAGAGACGCTGACTTCTGAAATATGCATAGTGTAA

Protein

MPGIHNVAIQGRLVEMLKKKNIKISIVIDQSGCNEPNDVFIKELLDAGAVIKYINTEPYTMQHKFCLIDDKVLMTGTLNWGNDRSSDHWNYVYITSKPKLVEPVSKEFKYMWLSSKDLNYSQFDSKPINEDECCNTEVVNLTENYKSVPFGNKETLTSEICIV

Description

Endonuclease that plays a critical role in PIWI-interacting RNA (piRNA) biogenesis during spermatogenesis. piRNAs provide essential protection against the activity of mobile genetic elements (By similarity). piRNA-mediated transposon silencing is thus critical for maintaining genome stability, in particular in germline cells when transposons are mobilized as a consequence of wide-spread genomic demethylation (By similarity). Has been proposed to act as a cardiolipin hydrolase to generate phosphatidic acid at mitochondrial surface (By similarity). Although it cannot be excluded that it can act as a phospholipase in some circumstances, it should be noted that cardiolipin hydrolase activity is either undetectable in vitro, or very low (PubMed:21397848). In addition, cardiolipin is almost exclusively found on the inner mitochondrial membrane, while PLD6 localizes to the outer mitochondrial membrane, facing the cytosol (PubMed:21397848). Has been shown to be a backbone-non-specific, single strand-specific nuclease, cleaving either RNA or DNA substrates with similar affinity. Produces 5' phosphate and 3' hydroxyl termini, suggesting it could directly participate in the processing of primary piRNA transcripts (By similarity). Also acts as a regulator of mitochondrial shape through facilitating mitochondrial fusion (PubMed:17028579, PubMed:26711011).

Subunit

Homodimer (PubMed:17028579). Interacts with MOV10L1 (By similarity). Interacts with MIGA1 and MIGA2; possibly facilitating homodimer formation (PubMed:26711011).

Similarity

Belongs to the phospholipase D family. MitoPLD/Zucchini subfamily.

Keywords

Complete proteome   Differentiation   Endonuclease   Hydrolase   Lipid degradation   Lipid metabolism   Meiosis   Membrane   Metal-binding   Mitochondrion   Mitochondrion outer membrane   Nuclease   Polymorphism   Reference proteome   Spermatogenesis   Transmembrane   Transmembrane helix   Zinc   Zinc-finger  

Feature

chain  Mitochondrial cardiolipin hydrolase
sequence variant  In dbSNP:rs17856924.

Uniprot

H9JR90   A0A194R3T5   A0A194PKY8   A0A212EI55   A0A2H1VYD5   S4PTC5   + More
A0A0L7KPJ2   A0A3S2TN45   A0A1E1XDP6   A0A023GD06   Q8N2A8   A0A2K5CG16   F6TQM8   A0A3B4YFF5   A0A3B4VN71   G3RV35   A0A2U9BQ98   A0A2R9AEN9   H2QCD0   A0A2R9AEB2   G2HE65   A0A383ZGQ1   A0A0S2I4X0   A0A091DAY7   I3KNT9   G5C332   A0A3Q7YAP4   A0A3L8Q451   G1RSR5   A0A3B5ARB5  

EC Number

3.1.-.-

Pubmed

19121390    26354079    22118469    23622113    26227816    28503490    + More
14702039    16625196    15489334    17028579    21397847    21397848    26711011    17495919    22398555    22722832    16136131    21484476    25186727    21993625    30282656   

EMBL

BABH01016273    KQ460779    KPJ12352.1    KQ459606    KPI91770.1    AGBW02014707    + More
OWR41151.1    ODYU01005213    SOQ45851.1    GAIX01012278    JAA80282.1    JTDY01007674    KOB65035.1    RSAL01000045    RVE50614.1    GFAC01001823    JAT97365.1    GBBM01003377    JAC32041.1    AK090899    AC055811    BC031263    CABD030039199    CP026250    AWP05796.1    AWP05797.1    AWP05798.1    AWP05799.1    AJFE02026409    AACZ04059834    AK305029    NBAG03000529    BAK62023.1    PNI17172.1    CP013118    ALO17377.1    KN122870    KFO27648.1    AERX01018863    JH173139    EHB15943.1    QUSF01011075    RLV62105.1    ADFV01121093   

Proteomes

UP000005204    UP000053240    UP000053268    UP000007151    UP000037510    UP000283053    + More
UP000005640    UP000233020    UP000002280    UP000261360    UP000261420    UP000001519    UP000246464    UP000240080    UP000002277    UP000261681    UP000064893    UP000028990    UP000005207    UP000006813    UP000286642    UP000276834    UP000001073    UP000261400   

PRIDE

H9JR90    Q8N2A8   

Pfam

PF13091   PLDc_2

Interpro

IPR025202   PLD-like_dom        + More
IPR001736   PLipase_D/transphosphatidylase       

ProteinModelPortal

H9JR90   A0A194R3T5   A0A194PKY8   A0A212EI55   A0A2H1VYD5   S4PTC5   + More
A0A0L7KPJ2   A0A3S2TN45   A0A1E1XDP6   A0A023GD06   Q8N2A8   A0A2K5CG16   F6TQM8   A0A3B4YFF5   A0A3B4VN71   G3RV35   A0A2U9BQ98   A0A2R9AEN9   H2QCD0   A0A2R9AEB2   G2HE65   A0A383ZGQ1   A0A0S2I4X0   A0A091DAY7   I3KNT9   G5C332   A0A3Q7YAP4   A0A3L8Q451   G1RSR5   A0A3B5ARB5  

PDB

4GGK     E-value=6.94311e-08,     Score=131

KEGG

101741648    K16862 mitochondrial cardiolipin hydrolase [EC:3.1.4.-] | (RefSeq) mitochondrial cardiolipin hydrola

GO

GO:0016787   GO:0016021   GO:0003824   GO:0010636   GO:0042803   GO:0016042   GO:0043046   GO:0030719   GO:0006654   GO:0007286   GO:0035755   GO:0005741   GO:0034587   GO:0051321   GO:0004519   GO:0046872   GO:0008053   GO:0070290   GO:0004630  

Subcellular location

Mitochondrion outer membrane  

Length:

163

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0

Exp number, first 60 AAs:

0

Total prob of N-in:

0.09364

outside

1  -  163

 

 

Pi

12.397678

Theta

15.929056

Tajima's D

-0.997964

CLR

7.904372

CSRT

0.137093145342733

Interpretation

Uncertain