Gene

KWMTBOMO06402  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA011965

Annotation

PREDICTED:_ATP-dependent_RNA_helicase_dbp2-like_isoform_X1_[Papilio_polytes]

Location

Bomo_Chr11(-):6463997-6473080      View in EpiBrowser

Full name

Cytoplasmic tRNA 2-thiolation protein 2      

Location in the cell

Cytoplasmic   Reliability : 1.102 Nuclear   Reliability : 1.004

CDS

ATGACGATAGACGTGGTGGCCGTGGTGGAAGAGGTGGGAGTCGCGGAGGGCGAGGATCCAGCCGCGGTGGTCGGGGTAGCGACAGGTCGCAAGAGCGCGGACCTAGTCGATTTAGCGGAGGTCGTGGTCGGGACGGTGGACGCGGCGGTAGGGATGGCGGCCGTGGAGGACGAGACAGCGGCAGGGGTTTTGGGAGAATGGGGAATCGGGATGATCGCTCCAATTTCAAAAATGATCAGCCTGGCGATAACTGTGAAAGGTCGAGACGTGCCTGCCCCTAGCATATTTTTTGAAGAGGGTGGTTTCCCTGACTATGCTATGAAAGAGATTTTAAAACAAGGTTTCCCGAACCCCACTCCTATTCAAGCACAGGGCTGGCCAATAGCTCTCTCTGGCCGTGACATGGTGGGCATAGCCCAAACTGGGTCAGGCAAAACCTTGGCATACATTTTACCAGCCATAGTACACATAATTAATCAACCGCGTCTGCTAAGAGATGATGGTCCAATAGTCCTTGTGTTGGCTCCAACAAGAGAGTTAGCCCAACAAATACAGCAGACCTTGATTGGGATGAAAGCGATCGCCCCTGAGTTGATGGACGATCCAGCCCCCATGGAAATTGTTGCCAATGAATTTGGTCAAAGTATCCACGTGCGGAACACTTGTATCTTTGGTGGTGCTCCAAAGGGACCACAGGGCAGATGTCTTGAGAGGGGAGTGGAAATTGTTATTGCTACTCCTGGTAGACTCATTGATTTTTTGGAGAAGGAGACAACGAACCTGCGCCGATGCACTTATTTGGTGCTGGATGAGGCTGATAGAATGTTGGACATGGGCTTCGAGCCTCAGATCAGGAAGATTATTGAACAGATTAGACCTGATAGACAAGTATTGATGTGGTCAGCTACGTGGCCCCGAGAAGTACAGAATCTAGCTGAAGAGTTCCTGCATGACTACATCCAGATCAACATTGGTTCTCTCGAGCTCTCGGCCAACCACAACATCCTCCAGATTGTTGATGTTTGTGAGGAATGGGAGAAGAATGATAAATTGATTACATTGCTTACTGAGATATCTTCAGAGGAGGAAACCAAAACTATTATATTTGCTGAAACCAAAAGGAGGGTTGACGACATATGTAAGGCGATAAACCGTGCCGGCTGGCGCGCCCTCGCCATCCACGGGGACAAGAACCAGCAGGACCGCGACTACGTGCTGAATCAGTTCCGCCACCACGCCGCCGCCATACTGGTCGCCACCGACGTCGCAGCCAGGGGACTCGATGTGGAAGATGTGAAGTTTGTGATAAACTATGACTATCCAAATAATTCTGAGGATTACGTGCATCGTATCGGCAGGACGGGACGGTCCCAAAACACCGGCACGGCCTACACACTCTTCACTCCTAATAATTCAGCAAAGGCTAGAGACTTGATGTCAGTACTTCAGGAAGCCAATCAAGTTGTGAATCCGAAACTACTAGAGTTGGCGCAATGTGGCATGGGATTCAAAGGAAAATTCGGTCGTTCAAGATACCGAGATGACGGCGGCAGGGGACGCGATGGAGGCTCACGCGGGGGACGCGGGAGTCGGGGTGGGGGCCGTGGTGGCTACGGCGACAGAGGTTCACGCTGGGACAACAGTAATGACAACCAAAACTCCTACAACGACTACAACAGGCAGAGTTTCGGCAATAGAGAGGGCGGATACCGCGGCAGGGGCCGGGGGAGGGGGGGACACGACGCGCCACAGACCTACTACAACATGTACTCACAACCGCCGCCCGCTCACTAG

Protein

MTIDVVAVVEEVGVAEGEDPAAVVGVATGRKSADLVDLAEVVVGTVDAAVGMAAVEDETAAGVLGEWGIGMIAPISKMISLAITVKGRDVPAPSIFFEEGGFPDYAMKEILKQGFPNPTPIQAQGWPIALSGRDMVGIAQTGSGKTLAYILPAIVHIINQPRLLRDDGPIVLVLAPTRELAQQIQQTLIGMKAIAPELMDDPAPMEIVANEFGQSIHVRNTCIFGGAPKGPQGRCLERGVEIVIATPGRLIDFLEKETTNLRRCTYLVLDEADRMLDMGFEPQIRKIIEQIRPDRQVLMWSATWPREVQNLAEEFLHDYIQINIGSLELSANHNILQIVDVCEEWEKNDKLITLLTEISSEEETKTIIFAETKRRVDDICKAINRAGWRALAIHGDKNQQDRDYVLNQFRHHAAAILVATDVAARGLDVEDVKFVINYDYPNNSEDYVHRIGRTGRSQNTGTAYTLFTPNNSAKARDLMSVLQEANQVVNPKLLELAQCGMGFKGKFGRSRYRDDGGRGRDGGSRGGRGSRGGGRGGYGDRGSRWDNSNDNQNSYNDYNRQSFGNREGGYRGRGRGRGGHDAPQTYYNMYSQPPPAH

Description

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with NCS6/CTU1 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.

Similarity

Belongs to the DEAD box helicase family.
Belongs to the CTU2/NCS2 family.

Uniprot

H9JR08   A0A2W1BWF1   A0A2H1W3Z1   A0A2A4J0D7   A0A2A4J0P9   A0A0N1IIL7   + More
K7J871   A0A1B0GM31   A0A2J7QBB9   Q17JB5   A0A1S4F0V4   B4N2Y4   A0A0R1DWB6   B4PJT1   B3NFL0   A0A0Q5UHM0   A0A1W4V8W9   A0A1L8DGB8   A0A1W4UWK9   A0A0J9RQ14   Q8MZI3   B4QJU9   A0A182H3B4   B3M9G0   B4LF26   A0A0V0G4H3   A0A0N8P154   B4K198   A0A182RFA1   T1D3U5   A0A182WIH7   E2C9Q7   B4KZU0   A0A182PHN0   A0A1L8EIE1   A0A1L8EI67   A0A084W474   A0A2J7QB62   A0A0M4EGX6   A0A2J7QB95   A0A182M4L2   A0A182J5D3   A0A182Y1A8   A0A182XMW2   A0A182UHX6   A0A182NGX0   A0A182UYD6   A0A1I8PT50   A0A182LJ37   A0A1L8EI11   B0X5U8   A0A2M4BF46   Q7PNS1   T1PCV1   A0A1I8MNM3   A0A067R366   A0A2M3YYG1   A0A182FHY4   E0VYW5   A0A2M3YYC0   A0A2M4A1K6   A0A2M4A1U9   A0A2M4CPI9   A0A182QFP4   W8B059   A0A0A1WXE6   A0A034VBD0   A0A2M3YYK3   A0A2J7QB73   A0A232EJE7   A0A154P740   K7J4U6  

Pubmed

19121390    28756777    26354079    20075255    17510324    17994087    + More
17550304    22936249    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    26109357    26109356    26483478    24330624    20798317    24438588    25244985    20966253    12364791    14747013    17210077    25315136    24845553    20566863    24495485    25830018    25348373    28648823   

EMBL

BABH01028569    BABH01028570    KZ149897    PZC78681.1    ODYU01006180    SOQ47810.1    + More
NWSH01004140    PCG65451.1    PCG65449.1    KQ460210    KPJ16586.1    AJVK01023899    AJVK01023900    AJVK01023901    NEVH01016301    PNF25859.1    CH477233    EAT46789.1    CH964062    EDW78723.2    CM000159    KRK01301.1    EDW93680.1    CH954178    EDV50552.1    KQS43344.1    GFDF01008684    JAV05400.1    CM002912    KMY97969.1    KMY97970.1    KMY97971.1    AE014296    AY102660    AAF50635.2    AAM27489.1    AGB94186.1    CM000363    EDX09490.1    JXUM01024463    JXUM01024464    JXUM01024465    JXUM01024466    JXUM01024467    KQ560691    KXJ81257.1    CH902618    EDV41173.1    CH940647    EDW69195.1    GECL01003162    JAP02962.1    KPU78972.1    CH916887    EDV90436.1    GALA01001238    JAA93614.1    GL453897    EFN75297.1    CH933809    EDW17952.1    GFDG01000420    JAV18379.1    GFDG01000428    JAV18371.1    ATLV01020284    KE525297    KFB45018.1    PNF25838.1    CP012525    ALC43752.1    PNF25839.1    AXCM01002623    GFDG01000419    JAV18380.1    DS232396    EDS41078.1    GGFJ01002528    MBW51669.1    AAAB01008960    EAA11703.5    KA646612    AFP61241.1    KK852729    KDR17555.1    GGFM01000536    MBW21287.1    DS235848    EEB18571.1    GGFM01000477    MBW21228.1    GGFK01001352    MBW34673.1    GGFK01001400    MBW34721.1    GGFL01003078    MBW67256.1    AXCN02000361    GAMC01012115    JAB94440.1    GBXI01010755    JAD03537.1    GAKP01019541    GAKP01019540    JAC39412.1    GGFM01000586    MBW21337.1    PNF25833.1    NNAY01004047    OXU18442.1    KQ434829    KZC07755.1    AAZX01007814   

Proteomes

UP000005204    UP000218220    UP000053240    UP000002358    UP000092462    UP000235965    + More
UP000008820    UP000007798    UP000002282    UP000008711    UP000192221    UP000000803    UP000000304    UP000069940    UP000249989    UP000007801    UP000008792    UP000001070    UP000075900    UP000075920    UP000008237    UP000009192    UP000075885    UP000030765    UP000092553    UP000075883    UP000075880    UP000076408    UP000076407    UP000075902    UP000075884    UP000075903    UP000095300    UP000075882    UP000002320    UP000007062    UP000095301    UP000027135    UP000069272    UP000009046    UP000075886    UP000215335    UP000076502   

PRIDE

H9JR08    Q8MZI3   

Pfam

PF00270   DEAD        + More
PF00271   Helicase_C
PF10288   CTU2

Interpro

IPR027417   P-loop_NTPase        + More
IPR001650   Helicase_C       
IPR014001   Helicase_ATP-bd       
IPR011545   DEAD/DEAH_box_helicase_dom       
IPR014014   RNA_helicase_DEAD_Q_motif       
IPR000629   RNA-helicase_DEAD-box_CS       
IPR019407   CTU2       
IPR014729   Rossmann-like_a/b/a_fold       

SUPFAM

SSF52540   SSF52540       

Gene 3D

3.40.50.620    

CDD

cd00079  HELICc

ProteinModelPortal

H9JR08   A0A2W1BWF1   A0A2H1W3Z1   A0A2A4J0D7   A0A2A4J0P9   A0A0N1IIL7   + More
K7J871   A0A1B0GM31   A0A2J7QBB9   Q17JB5   A0A1S4F0V4   B4N2Y4   A0A0R1DWB6   B4PJT1   B3NFL0   A0A0Q5UHM0   A0A1W4V8W9   A0A1L8DGB8   A0A1W4UWK9   A0A0J9RQ14   Q8MZI3   B4QJU9   A0A182H3B4   B3M9G0   B4LF26   A0A0V0G4H3   A0A0N8P154   B4K198   A0A182RFA1   T1D3U5   A0A182WIH7   E2C9Q7   B4KZU0   A0A182PHN0   A0A1L8EIE1   A0A1L8EI67   A0A084W474   A0A2J7QB62   A0A0M4EGX6   A0A2J7QB95   A0A182M4L2   A0A182J5D3   A0A182Y1A8   A0A182XMW2   A0A182UHX6   A0A182NGX0   A0A182UYD6   A0A1I8PT50   A0A182LJ37   A0A1L8EI11   B0X5U8   A0A2M4BF46   Q7PNS1   T1PCV1   A0A1I8MNM3   A0A067R366   A0A2M3YYG1   A0A182FHY4   E0VYW5   A0A2M3YYC0   A0A2M4A1K6   A0A2M4A1U9   A0A2M4CPI9   A0A182QFP4   W8B059   A0A0A1WXE6   A0A034VBD0   A0A2M3YYK3   A0A2J7QB73   A0A232EJE7   A0A154P740   K7J4U6  

PDB

3FE2     E-value=4.59879e-90,     Score=846

KEGG

101743582    K12823 ATP-dependent RNA helicase DDX5/DBP2 [EC:3.6.4.13] | (RefSeq) ATP-dependent RNA helicase dbp2

PATHWAY

03040    Spliceosome - Bombyx mori (domestic silkworm)

GO

GO:0003676   GO:0004386   GO:0005524   GO:0003724   GO:0016779   GO:0000049   GO:0034227   GO:0005737   GO:0002098   GO:0032447   GO:0015986   GO:0051536   GO:0016740   GO:0043412   GO:0015074   GO:0015923   GO:0005634   GO:0006260  

Subcellular location

Cytoplasm  

Length:

597

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.16501

Exp number, first 60 AAs:

0.00759

Total prob of N-in:

0.00746

outside

1  -  597

 

 

Pi

207.983735

Theta

170.990135

Tajima's D

0.016967

CLR

0.978276

CSRT

0.376831158442078

Interpretation

Uncertain