SGID Silkworm Genome Informatics Database
Gene
KWMTBOMO06399  Validated by peptides from experiments
Pre Gene Modal
BGIBMGA011966
Annotation
CDKAL1-like_protein_[Papilio_xuthus]
Full name
Xylose isomerase       + More
Threonylcarbamoyladenosine tRNA methylthiotransferase      
Alternative Name
CDKAL1-like protein
tRNA-t(6)A37 methylthiotransferase
CDK5 regulatory subunit-associated protein 1-like 1
Location in the cell
Cytoplasmic   Reliability : 2.118
 

Sequence

CDS
ATGCCAGGTGTTTGTGATGAAATTATAGACGACATAGAAGATTTGATATCTTCCCAGGATATAACTCCTAAAGAACGTTATGCTAGTAGGAAGAATGTAAGCGTCCGATCTAAAAAGAGGGAGAAAAAAGACCCCGAACAGATTGAAAAGGTTATATTAGAGAGTGTTGTACCAGGCACTCAAACAATATATGTAAAAACATGGGGCTGTGCTCATAACAACTCAGATTCAGAGTACATGGCCGGACTGCTGGCTGCTAATGGCTACAAATTGACAGAAGACAAATGGGATGCGCAACTTTGGTTGTTGAATTCATGTACTGTAAAGAGCCCGGCTGAGGATCATTTCAAGAATGAAATTGAGCTTGGTCAGAGTCGTGGCATACATGTTGTTGTAGCAGGCTGTGTACCGCAGGGCGCACCAAAAAGTGGCTACCTACATGGACTCAGTATAGTTGGTGTACAACAGATTGACAGAATTGTGGAGGTCGTGGAGGAGACTTTGAAAGGTCACACAGTCCGTCTGTTTGGTCAGAGGAAAACAAACGGTCGTAAAGCGGGTGGGGCTTCACTGCTCCTGCCCAAAGTGCGCAAAAATCCTTTAGTCGAGATCATAGCCGTGAACACGGGCTGCCTGAACCAGTGCACATACTGCAAGACCAAGCACGCCAGAGGAGAGCTGGGCAGCTACCCTCCGGAAGAGATCGTGGAGAGAGCACGACAGTCGTTCACCGAGGGAGTTGTGGAGATATGGCTTACAAGCGAGGATACAGGAACCTACGGTCGTGACATAGGAACATCTCTGCCTGCCCTGCTCGATGCGCTAGTATCAGTGATACCGGACGGCACGAGGCTCCGTCTGGGCATGACCAACCCTCCCTACATCCTGGAACACCTGCCCGCTGTCGCGAGGATCATGAGGCACCCCAGGGTCTACAAGTTTCTCCACGTCCCTGTGCAGTCGGCCAGTGACCAGGTACTGTCCGACATGAAGAGGGAATACACGCGAGCCGACTTCGAACACGTGGTCGACTATCTCAGGAAAGAAGTCCCCGGCATCACGATAGCCACAGACATCATCTGCGGCTTCCCCACTGAGACCGAAGCTGACTTCGAGGAGACCATGCAGCTCTGCAGAAAGTACAAGTTCCCTTCGTTGTTCATCAACCAGTTCTTCCCGAGACCCGGGACGCCGGCTGCCAAGATGACCAGGGTACCTGGTCAAGAAGTAAAGAAACGCACAAAATTACTGTCGGACTTCTTCAGGTCGTACGAACCTTACGGTCACAAGGTGGGGGAGACGCAGGAGGTGCTAGTGACCGACGTGTCGCACGACAAGAACTACTTTGTCGGCCACAACGAGTTCTACGAGCAAGTACTGATACCTAAGGAGGAGAAGTACATGGGGAAATTAGTTACAGTAAAGATTACGAGCGCCAGTAAATTCTCTATGATGGGTCAGCCCATAGACAAACCTAAGATGGCGGGCTTGACGGTGCCTTTGAAGAAAGGAGAGCTTTCCGGTGTCTATGGTTTGAAAAGGGAAAATAAAATACCTATCCCTATACTTGTGCTGTTGATGGTTGTCTTAGCTAGATTTATATGGATGTTTTTTATAGTTTAA
Protein
MPGVCDEIIDDIEDLISSQDITPKERYASRKNVSVRSKKREKKDPEQIEKVILESVVPGTQTIYVKTWGCAHNNSDSEYMAGLLAANGYKLTEDKWDAQLWLLNSCTVKSPAEDHFKNEIELGQSRGIHVVVAGCVPQGAPKSGYLHGLSIVGVQQIDRIVEVVEETLKGHTVRLFGQRKTNGRKAGGASLLLPKVRKNPLVEIIAVNTGCLNQCTYCKTKHARGELGSYPPEEIVERARQSFTEGVVEIWLTSEDTGTYGRDIGTSLPALLDALVSVIPDGTRLRLGMTNPPYILEHLPAVARIMRHPRVYKFLHVPVQSASDQVLSDMKREYTRADFEHVVDYLRKEVPGITIATDIICGFPTETEADFEETMQLCRKYKFPSLFINQFFPRPGTPAAKMTRVPGQEVKKRTKLLSDFFRSYEPYGHKVGETQEVLVTDVSHDKNYFVGHNEFYEQVLIPKEEKYMGKLVTVKITSASKFSMMGQPIDKPKMAGLTVPLKKGELSGVYGLKRENKIPIPILVLLMVVLARFIWMFFIV

Summary

Description
Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.
Catalytic Activity
D-xylose = D-xylulose
[sulfur carrier]-SH + AH2 + N(6)-L-threonylcarbamoyladenosine(37) in tRNA + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H(+) + L-methionine + S-adenosyl-L-homocysteine
Cofactor
[4Fe-4S] cluster
Similarity
Belongs to the xylose isomerase family.
Belongs to the methylthiotransferase family. CDKAL1 subfamily.
Belongs to the E2F/DP family.
Keywords
4Fe-4S   Complete proteome   Iron   Iron-sulfur   Membrane   Metal-binding   Reference proteome   S-adenosyl-L-methionine   Transferase   Transmembrane   Transmembrane helix   tRNA processing   Endoplasmic reticulum  
Feature
chain  Threonylcarbamoyladenosine tRNA methylthiotransferase
EC Number
5.3.1.5
2.8.4.5
EMBL
BABH01028575    KQ459590    KPI97155.1    NWSH01001334    PCG71649.1    ODYU01003295    + More
SOQ41864.1    GAIX01003695    JAA88865.1    HQ199330    ADX30521.1    KZ149897    PZC78684.1    AGBW02008998    OWR51900.1    KQ971319    EFA11366.1    KQ980658    KYN14779.1    KK107148    QOIP01000009    EZA57454.1    RLU18877.1    GL762326    EFZ21687.1    KQ981382    KYN42150.1    NEVH01024946    PNF16584.1    KQ977279    KYN04254.1    GL434696    EFN74642.1    KQ435802    KOX73195.1    KZ288186    PBC34863.1    LBMM01000671    KMQ97823.1    KQ434822    KZC07211.1    GL888218    EGI64557.1    GEZM01042817    JAV79573.1    KQ414805    KOC60637.1    KQ976464    KYM84417.1    ADTU01022953    KB631869    ERL86836.1    GL732523    EFX90271.1    CH964291    EDW86282.1    GDIQ01148434    JAL03292.1    OUUW01000001    SPP74689.1    GDIP01101779    JAM01936.1    GDIQ01028109    JAN66628.1    GDIP01191974    GDIP01049187    LRGB01000248    JAJ31428.1    KZS20098.1    SPP74688.1    GDIQ01053862    JAN40875.1    GDIP01231227    JAI92174.1    CH902619    EDV36760.1    GDIP01101780    JAM01935.1    GDIP01118449    JAL85265.1    GEDC01020923    JAS16375.1    AJWK01035490    CH940648    EDW61309.1    CH916367    EDW01367.1    AJVK01035195    CH954179    EDV55626.1    GDRN01060569    JAI65341.1    AE013599    AY051634    GEBQ01029378    JAT10599.1    NNAY01004341    OXU18031.1    CM000362    CM002911    EDX07481.1    KMY94531.1    CH480816    EDW48279.1    AJVK01058655    GDIQ01132638    JAL19088.1    CH477765    EAT36482.1    CP012524    ALC41087.1    DS469592    EDO40392.1    GDAI01002176    JAI15427.1    GL448301    EFN84849.1    GFDL01013051    JAV21994.1    GECZ01031130    JAS38639.1    GECZ01012989    JAS56780.1    NEDP02005572    OWF38160.1    DS231817    EDS39644.1    GAMC01006390    JAC00166.1    CCAG010010868    GBBI01002678    JAC16034.1    JW866821    AFO99338.1    GBGD01001101    JAC87788.1    CH933808    EDW10355.1    JXJN01024553    CH479181    EDW32008.1    CM000071    CM004477    OCT74519.1    GFTR01006784    JAW09642.1    GBYX01462234    JAO19351.1    BC070521    ACPB03004219    KK735815    KFR15987.1    AGTO01010359    GBXI01017426    JAC96865.1    LSYS01007707    OPJ71323.1    KB742735    EOB04920.1    HADY01006470    HAEJ01003746    SBS44203.1    MRZV01001275    PIK39117.1   
Pfam
PF00919   UPF0004        + More
PF04055   Radical_SAM
PF01938   TRAM
PF16421   E2F_CC-MB
PF02319   E2F_TDP
Interpro
IPR013848   Methylthiotransferase_N        + More
IPR002792   TRAM_dom       
IPR020612   Methylthiotransferase_CS       
IPR038135   Methylthiotransferase_N_sf       
IPR005839   Methylthiotransferase       
IPR023404   rSAM_horseshoe       
IPR007197   rSAM       
IPR006466   MiaB-like_B       
IPR006638   Elp3/MiaB/NifB       
IPR036237   Xyl_isomerase-like_sf       
IPR001998   Xylose_isomerase       
IPR032198   E2F_CC-MB       
IPR037241   E2F-DP_heterodim       
IPR036390   WH_DNA-bd_sf       
IPR036388   WH-like_DNA-bd_sf       
IPR003316   E2F_WHTH_DNA-bd_dom       
SUPFAM
SSF51658   SSF51658        + More
SSF144074   SSF144074       
SSF46785   SSF46785       
Gene 3D
PDB
4JC0     E-value=1.35291e-25,     Score=290

Ontologies

Topology

Subcellular location
Membrane  
Endoplasmic reticulum membrane  
Nucleus  
Length:
540
Number of predicted TMHs:
1
Exp number of AAs in TMHs:
20.0444
Exp number, first 60 AAs:
0
Total prob of N-in:
0.01860
outside
1  -  519
TMhelix
520  -  539
inside
540  -  540
 
 

Population Genetic Test Statistics

Pi
179.104753
Theta
165.620972
Tajima's D
0.596461
CLR
0.274937
CSRT
0.544222788860557
Interpretation
Uncertain
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