Gene

KWMTBOMO06377  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA011922

Annotation

alcohol_dehydrogenase_[Bombyx_mori]

Location

Bomo_Chr11(+):5944611-5946053      View in EpiBrowser

Full name

S-(hydroxymethyl)glutathione dehydrogenase       + More
Alcohol dehydrogenase class-3      

Alternative Name

Alcohol dehydrogenase class-III
Glutathione-dependent formaldehyde dehydrogenase
S-(hydroxymethyl)glutathione dehydrogenase

Location in the cell

Cytoplasmic   Reliability : 4.036

CDS

ATGTCAACAGTCGGTAAAGTGATTAAGTGCTTGGCCGCAGTCGCGTGGGAAGCAGGCAAGCCGCTGTCCATCGAGGAGATTGAAGTGGACCCGCCAAAAGCCGGTGAAGTGCGCGTTAAGATCACGGCCACCGGAGTCTGCCATACTGACGCGTATACACTCTCCGGAAAAGATCCTGAGGGAGTGTTTCCTGTAGTACTGGGACATGAAGGCGGCGGAATCGTGGAGAGTGTCGGTGAGGGAGTCACTTCAGTCAAGCCCGGGGACCACGTAGTACCTCTGTACGTCCCACAGTGCAACACATGTAAATTCTGCTTGAATCCGAAAACTAATTTGTGCCAGAAGGTTCGTTCTACTCAAGGTCAAGGTGTGATGCCAGATGGCACTAGGCGATTCCGCTGTAAAGGACAGGAACTCTACCATTTCATGGGTTGTTCAACATTCAGTCAGTACACAGTTGTTCTAGAAATTTCTCTCTGTAAAGTTGCAGAGGCTGCTCCATTAGATAAAGTTTGTTTGCTGGGATGCGGTGTACCTACAGGTTATGGAGCCGCCTTGAATACTGCCAAAGTTGAACCAGGATCAAATTGCGCTATTTTTGGTCTTGGTGCTGTTGGTTTAGCTGTTGCTCTTGGATGCAAAGCGGCAGGTGCCAATCGCATTATTGGTGTTGACATCAACCCTGACAAGTTTGAGGTAGCTAAGAAATTTGGAGTCAATGAATTTGTCAACCCTAAGGATTATGATAAACCAATTCAACAAGTACTGGTGGATTTGACTGATGGTGGTCTAGAATACACTTTTGAATGTATTGGAAATGTAGGCACCATGAGAGCTGCACTAGAAGCTTGCCATAAGGGATGGGGTGTGTCAGTGATCATTGGTGTAGCTGCTGCTGGAGAAGAGATCAGCACTCGTCCATTCCAACTTGTTACAGGTCGCACCTGGAAGGGAACAGCTTTTGGAGGTTACAAAAGTAGAGAAAGTGTACCAAAGCTTGTAGATGAGTACTTGGAGAAGAAGCTGCCTTTAGACGAATTTGTCACTCACAATGTGCCGCTGAAGGAGATCAATGAGGCATTCCATTTGATGCATGCTGGAAAATCTATCCGTGCAGTAGTTGACATGTAA

Protein

MSTVGKVIKCLAAVAWEAGKPLSIEEIEVDPPKAGEVRVKITATGVCHTDAYTLSGKDPEGVFPVVLGHEGGGIVESVGEGVTSVKPGDHVVPLYVPQCNTCKFCLNPKTNLCQKVRSTQGQGVMPDGTRRFRCKGQELYHFMGCSTFSQYTVVLEISLCKVAEAAPLDKVCLLGCGVPTGYGAALNTAKVEPGSNCAIFGLGAVGLAVALGCKAAGANRIIGVDINPDKFEVAKKFGVNEFVNPKDYDKPIQQVLVDLTDGGLEYTFECIGNVGTMRAALEACHKGWGVSVIIGVAAAGEEISTRPFQLVTGRTWKGTAFGGYKSRESVPKLVDEYLEKKLPLDEFVTHNVPLKEINEAFHLMHAGKSIRAVVDM

Description

Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione.

Catalytic Activity

NAD(+) + S-(hydroxymethyl)glutathione = H(+) + NADH + S-formylglutathione
a primary alcohol + NAD(+) = an aldehyde + H(+) + NADH
a secondary alcohol + NAD(+) = a ketone + H(+) + NADH
NADP(+) + S-(hydroxymethyl)glutathione = H(+) + NADPH + S-formylglutathione

Cofactor

Zn(2+)

Subunit

Homodimer.

Similarity

Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.

Keywords

Acetylation   Cytoplasm   Direct protein sequencing   Metal-binding   NAD   Oxidoreductase   Zinc  

Feature

chain  Alcohol dehydrogenase class-3

Uniprot

Q1HQD0   G0T416   G0T422   G0T406   H9JQW6   G0T414   + More
G0T409   G0T410   G0T407   G0T415   A0A076FRN7   A0A0F6Q4I6   A0A0F6Q4J2   A0A0N1IQ91   A0A2H1WL75   I4DQ77   A0A0L7L6G7   A0A3S2NH17   A0A212EJ02   A0A0H4TH30   A0A0F7QEC5   A0A1L8D6N0   S4PWU5   A0A2H4V6Y4   Q6NXA6   Q90XD4   A0A2J7RIC3   A0A067QVS5   A0A3B4E885   W5L8R5   A0A3B3YF14   A0A3B3DT76   W5N326   A0A3B3VPD2   M3ZJE0   A0A3P9P0T2   A0A087YMH6   A0A3Q2T0S8   A0A0S7HHV8   A0A1C9TA53   A0A0A9Z321   A0A3B5M8F4   A0A3Q2DLU3   A0A3P8Z109   A0A1A8MGI4   Q6R5I9   A0A1A8PST8   A0A1A8JYA5   A0A1A8DLD2   A0A1A8UAE9   A0A3B3I8V0   K1QKF8   A0A3B4H2F5   A0A3B3R2H8   A0A1A7YAV9   A0A2I4APN8   A0A3Q4MTV0   A0A1A8H6F7   I3IXM8   A0A3P9ITG3   A0A3P8PCH6   A0A0P4WSQ3   A0A3Q3W9B2   A0A3Q1BR70   W5UFF6   A0A3Q1FHK9   A0A3B4ZFY7   A0A0F8C7C9   A0A146NLA1   A0A146VHK9   C1BKF9   A0A3Q3FMP1   A0A3P9P0U6   A0A2U9C806   A0A2D0RJM7   A0A1W5AN90   A0A3P9KC24   P80467   A0A3B4YNT3   A0A1A8F7C6   A0A3P9AV78   A0A3Q3W5V7   A0A315V5L8   H2LKI1   A0A3P8TGE2   A0A087T8A5   A0A3Q2Z6H5   A0A1S3JMK7   P79896   H3C205   A0A3B5AE30   A0A1L8EDP6   J3JW17   P86884   B5XA77   Q9NJC3   A0A3Q1FC45   W0UFJ5   Q9NJD0   A0A182GHU2  

EC Number

1.1.1.284
1.1.1.1

Pubmed

22239062    19121390    24817326    28756777    26354079    22651552    + More
26227816    22118469    25803580    23622113    23594743    11527411    24845553    25329095    29451363    23542700    18574105    25401762    26823975    25069045    15763521    17554307    22992520    29240929    25186727    23127152    25835551    7589468    29703783    8863743    15496914    22516182    23537049    20433749    11054102    12087171    26483478   

EMBL

DQ443122    GQ420793    GQ420795    GQ420796    GQ420797    GQ420807    + More
GQ420808    GQ420812    GQ420814    GQ420815    GQ420816    GQ420820    ABF51211.1    ADM32136.1    ADM32138.1    ADM32139.1    ADM32140.1    ADM32150.1    ADM32151.1    ADM32155.1    ADM32157.1    ADM32158.1    ADM32159.1    ADM32163.1    GQ420794    GQ420798    GQ420799    GQ420800    GQ420803    GQ420806    GQ420811    GQ420813    GQ420818    GQ420819    ADM32137.1    ADM32141.1    ADM32142.1    ADM32143.1    ADM32146.1    ADM32149.1    ADM32154.1    ADM32156.1    ADM32161.1    ADM32162.1    GQ420817    ADM32160.1    GQ420801    ADM32144.1    BABH01028609    BABH01028610    GQ420809    ADM32152.1    GQ420804    ADM32147.1    GQ420805    ADM32148.1    GQ420802    ADM32145.1    GQ420810    ADM32153.1    KF960798    AII22000.1    KP237885    KZ149897    AKD01738.1    PZC78697.1    KP237890    AKD01743.1    KQ459692    KPJ20427.1    ODYU01009419    SOQ53831.1    AK403971    BAM20067.1    JTDY01002659    KOB70995.1    RSAL01000104    RVE47362.1    AGBW02014545    OWR41467.1    KP899555    AKQ06154.1    LC017751    BAR64765.1    GEYN01000010    JAV02119.1    GAIX01006178    JAA86382.1    KY130514    AUB45375.1    CU407330    CU467639    BC067170    AAH67170.1    AF399909    AAL26325.1    NEVH01003502    PNF40582.1    KK852898    KDR14152.1    AHAT01017724    AYCK01004224    GBYX01440886    JAO40485.1    KU553321    AOR07019.1    GBHO01003982    GBHO01003980    GBHO01003979    GBHO01003977    GBRD01013281    GDHC01017979    GDHC01001441    JAG39622.1    JAG39624.1    JAG39625.1    JAG39627.1    JAG52545.1    JAQ00650.1    JAQ17188.1    HAEF01014779    HAEG01003256    SBR55938.1    AY512892    AAS15570.1    HAEH01008388    HAEI01009543    SBR84336.1    HAED01014738    HAEE01005105    SBR25125.1    HADZ01012531    HAEA01005858    SBQ34338.1    HADY01009351    HAEJ01004802    SBS45259.1    JH817156    EKC37227.1    HADW01016942    HADX01005144    SBP27376.1    HAEC01010817    SBQ79033.1    AERX01009881    GDRN01029963    JAI67580.1    JT414681    AHH40718.1    KQ041511    KKF26047.1    GCES01153824    JAQ32498.1    GCES01069409    JAR16914.1    BT075088    ACO09512.1    CP026255    AWP11749.1    HAEB01007487    SBQ54014.1    NHOQ01002284    PWA18600.1    KK113910    KFM61344.1    U84791    GFDG01001961    JAV16838.1    APGK01038541    APGK01038542    BT127435    KB740960    KB631679    AEE62397.1    ENN77017.1    ERL85342.1    BT047946    ACI67747.1    AH009402    AF156698    AAF73255.1    HF571259    CCQ25769.1    AF154331    AAF73254.1    JXUM01064752    JXUM01064753    KQ562313    KXJ76169.1   

Proteomes

UP000005204    UP000053240    UP000037510    UP000283053    UP000007151    UP000000437    + More
UP000235965    UP000027135    UP000261440    UP000018467    UP000261480    UP000261560    UP000018468    UP000261500    UP000002852    UP000242638    UP000028760    UP000265000    UP000261380    UP000265020    UP000265140    UP000001038    UP000005408    UP000261460    UP000261540    UP000192220    UP000261580    UP000005207    UP000265200    UP000265100    UP000261620    UP000257160    UP000257200    UP000261400    UP000264800    UP000246464    UP000221080    UP000192224    UP000265180    UP000261360    UP000265160    UP000265080    UP000054359    UP000264820    UP000085678    UP000007303    UP000019118    UP000030742    UP000087266    UP000069940    UP000249989   

PRIDE

G0T416    G0T422    G0T406    H9JQW6    G0T414    G0T409    + More
G0T410    G0T407    G0T415    P80467    P86884   

Pfam

PF00107   ADH_zinc_N        + More
PF08240   ADH_N

Interpro

IPR014183   ADH_3        + More
IPR036291   NAD(P)-bd_dom_sf       
IPR011032   GroES-like_sf       
IPR013149   ADH_C       
IPR002328   ADH_Zn_CS       
IPR013154   ADH_N       
IPR020843   PKS_ER       

SUPFAM

SSF50129   SSF50129        + More
SSF51735   SSF51735       

CDD

cd08300  alcohol_DH_class_III

ProteinModelPortal

Q1HQD0   G0T416   G0T422   G0T406   H9JQW6   G0T414   + More
G0T409   G0T410   G0T407   G0T415   A0A076FRN7   A0A0F6Q4I6   A0A0F6Q4J2   A0A0N1IQ91   A0A2H1WL75   I4DQ77   A0A0L7L6G7   A0A3S2NH17   A0A212EJ02   A0A0H4TH30   A0A0F7QEC5   A0A1L8D6N0   S4PWU5   A0A2H4V6Y4   Q6NXA6   Q90XD4   A0A2J7RIC3   A0A067QVS5   A0A3B4E885   W5L8R5   A0A3B3YF14   A0A3B3DT76   W5N326   A0A3B3VPD2   M3ZJE0   A0A3P9P0T2   A0A087YMH6   A0A3Q2T0S8   A0A0S7HHV8   A0A1C9TA53   A0A0A9Z321   A0A3B5M8F4   A0A3Q2DLU3   A0A3P8Z109   A0A1A8MGI4   Q6R5I9   A0A1A8PST8   A0A1A8JYA5   A0A1A8DLD2   A0A1A8UAE9   A0A3B3I8V0   K1QKF8   A0A3B4H2F5   A0A3B3R2H8   A0A1A7YAV9   A0A2I4APN8   A0A3Q4MTV0   A0A1A8H6F7   I3IXM8   A0A3P9ITG3   A0A3P8PCH6   A0A0P4WSQ3   A0A3Q3W9B2   A0A3Q1BR70   W5UFF6   A0A3Q1FHK9   A0A3B4ZFY7   A0A0F8C7C9   A0A146NLA1   A0A146VHK9   C1BKF9   A0A3Q3FMP1   A0A3P9P0U6   A0A2U9C806   A0A2D0RJM7   A0A1W5AN90   A0A3P9KC24   P80467   A0A3B4YNT3   A0A1A8F7C6   A0A3P9AV78   A0A3Q3W5V7   A0A315V5L8   H2LKI1   A0A3P8TGE2   A0A087T8A5   A0A3Q2Z6H5   A0A1S3JMK7   P79896   H3C205   A0A3B5AE30   A0A1L8EDP6   J3JW17   P86884   B5XA77   Q9NJC3   A0A3Q1FC45   W0UFJ5   Q9NJD0   A0A182GHU2  

PDB

3QJ5     E-value=1.66421e-141,     Score=1288

KEGG

733095    K00121 S-(hydroxymethyl)glutathione dehydrogenase / alcohol dehydrogenase [EC:1.1.1.284 1.1.1.1] | (

PATHWAY

00010    Glycolysis / Gluconeogenesis - Bombyx mori (domestic silkworm)
00071    Fatty acid degradation - Bombyx mori (domestic silkworm)
00350    Tyrosine metabolism - Bombyx mori (domestic silkworm)
00830    Retinol metabolism - Bombyx mori (domestic silkworm)
00980    Metabolism of xenobiotics by cytochrome P450 - Bombyx mori (domestic silkworm)
00982    Drug metabolism - cytochrome P450 - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)
01200    Carbon metabolism - Bombyx mori (domestic silkworm)

GO

GO:0008270   GO:0051903   GO:0006069   GO:0046872   GO:0046294   GO:0005829   GO:0004024   GO:0005737   GO:0004022   GO:0016491   GO:0016310   GO:0016627   GO:0030145   GO:0015923   GO:0005634   GO:0006260   GO:0055114  

Subcellular location

Cytoplasm  

Length:

376

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

1.23043

Exp number, first 60 AAs:

0.00037

Total prob of N-in:

0.06873

outside

1  -  376

 

 

Pi

37.406121

Theta

64.174653

Tajima's D

-1.33374

CLR

232.939621

CSRT

0.0817459127043648

Interpretation

Uncertain