Gene

KWMTBOMO06367

Pre Gene Modal

BGIBMGA011979

Annotation

PREDICTED:_serine/threonine-protein_kinase_SMG1-like_[Bombyx_mori]

Location

Bomo_Chr11(-):5801067-5806890      View in EpiBrowser

Full name

Non-specific serine/threonine protein kinase       + More
Serine/threonine-protein kinase SMG1      

Alternative Name

61E3.4
Lambda/iota protein kinase C-interacting protein

Location in the cell

Cytoplasmic   Reliability : 1.697 Golgi   Reliability : 1.631

CDS

ATGGTCCACACTGGCATATTCCGTCTCGCCTGCGAGCACGTGTTGCGCACGATGCGTCGTGGCCGCGAAACCCTGTTGACGTTGTTGGAAGCGTTCGTGTACGATCCGCTGGTCGAATGGGGCGGCAGCAGCGGAGGGAAGCGACGCCGCACGCAGAGAGACGTTAAATCGGCTTTGGACATGATGGCGGTGAGAGCACAAGAATTGCAACATAGTCTGGCCCAAGTCACAGAACAATTTCTGGCCATCTTGCCTGGTATCATTGAAAGCGCTGACAAGTGGCAAAAAGAACATGAAGAACTGGTTGAAGTCGAGGCGAGACTCCAAGATTGCCACCAACAAATGGCACTAATCAAAGAAATAGAAGCATATGGACCTAATCTGAACAACCATCCATTGCACGCAATCTCGCAAAAGTACTCTTCATACAAACAAGCTAAGAATGCTGTTGAAGATTCCAAAAAAGCGTTAGTCAAAATACTGAATGATTTTGACGCACAGATCGAAAGTTTCTCAGCGACTTCTGAACTTCTTAATGGTCCGCAATTGATGGCTTGGGTGCAAGAATTTTCCGCTCCAAACGAAGAAGAAGACACGCCGATCTTTGAACACATAAAAGATTTTCTAACAAATGCAGGTCAAAGCTCCATGATCACTCAATGCGAACAAGCCGAAAAAGAATTTTATCAATCTTTGAAACAGACCCAGTGCATCATTAGGGCTTGTCTGGAACTTTTGTCACAATACGTAGCTGTCTCTCAATATTTCCCACAGAGTCAAACGGAATACCATCGCATAGTTATGTTCCGGAAGTTCCTTGCTGCTGCTCTAGACAGCAAATCGCCCGAGGTTTGCCGAGAGGTTGCCAGTCAAGTCAACGCCATCATCAATGCGGAGAACAACAAAGGCGATCCGCAACAAATTATTGCTTATAATTATCGCTTGGAAACCATCAGTGCTAAAGCGAACGCAAACTTGGCTAAGTGTGTTGAGAAGTTGCAACTAGAAGGTGGACCAGAAGCCATGGCTGTTGCTCAAGAAGCTTACAGAGAGGCAAAGGCGAGCATCGGCAACTGGGTCAGGTCTGAAGAAGGAGCCGCAACCGCTTTAGAATGTGCTGTTATCAGCATGCTGTGTCATTTAAATCGAAGATATCTGATGCTTGAGAGCGGTGCCCAAAGTGCTGGTGATTGTCTCGTGGACTTAACGTCGAGAGAAGGAGAGTGGTTCCTAGATGATATGAGCGCTTTGTCGACCCAAGCTGTGGAGCTTTTATCGCTGCTGCCGTTGCAGTCTGCTTCAGTAGAAGACGCTGCTTTACCAGTAGCAGTGGAATGTGTTCGGAATGTGAATTACCTGCTAGCTGATTTGGTCCAACTAAATTACAATTTCAGCACGATCATACTTCCTGAAGCGTTAAAGAAGATACATTCTGAAGAACCGTCTGTCCTACTAATGATAAATGAACTGAATGTGGTCATAATGAATTCTACTGTGCCTTTGAACGAGTTATTGGCCCAGCTTGAAGTGCATCTTAGATACTTGGTCATGGATATGGAGTCTCCAGCAAGCAGCGCTCAAGTTGTAGCGGCAGAACTGCGTGCTCGTTATGAAGCACTGCTTTCTGCCCCGACTTCTGATGTGGAGGGACAGTCTTCAGGACGAATGCTGCTTATGGGTTTCAACGGTCTATTCGCTGCTGTAGAATTGAGAGGAAGGGAACTGGCCGACCACTTGGCAATACCTATACCTCCAGCTTGGAGGAAGATTGATCATATCAGCGAATCTATGCACATGTCTGTAAGTGTAGTTTGTTTGTTTGTTTATTTATATTTCTTGGCCATCAATGTTAAATAA

Protein

MVHTGIFRLACEHVLRTMRRGRETLLTLLEAFVYDPLVEWGGSSGGKRRRTQRDVKSALDMMAVRAQELQHSLAQVTEQFLAILPGIIESADKWQKEHEELVEVEARLQDCHQQMALIKEIEAYGPNLNNHPLHAISQKYSSYKQAKNAVEDSKKALVKILNDFDAQIESFSATSELLNGPQLMAWVQEFSAPNEEEDTPIFEHIKDFLTNAGQSSMITQCEQAEKEFYQSLKQTQCIIRACLELLSQYVAVSQYFPQSQTEYHRIVMFRKFLAAALDSKSPEVCREVASQVNAIINAENNKGDPQQIIAYNYRLETISAKANANLAKCVEKLQLEGGPEAMAVAQEAYREAKASIGNWVRSEEGAATALECAVISMLCHLNRRYLMLESGAQSAGDCLVDLTSREGEWFLDDMSALSTQAVELLSLLPLQSASVEDAALPVAVECVRNVNYLLADLVQLNYNFSTIILPEALKKIHSEEPSVLLMINELNVVIMNSTVPLNELLAQLEVHLRYLVMDMESPASSAQVVAAELRARYEALLSAPTSDVEGQSSGRMLLMGFNGLFAAVELRGRELADHLAIPIPPAWRKIDHISESMHMSVSVVCLFVYLYFLAINVK

Description

Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate p53/TP53 and is required for optimal p53/TP53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR). May activate PRKCI but not PRKCZ (By similarity).
Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate p53/TP53 and is required for optimal p53/TP53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR). May activate PRKCI but not PRKCZ.

Catalytic Activity

ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]
ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]

Cofactor

Mn(2+)

Subunit

Component of the SMG1C complex composed of SMG1, SMG8 and SMG9; the recruitment of SMG8 to SMG1 N-terminus induces a large conformational change in the SMG1 C-terminal head domain containing the catalytic domain. Component of the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. Interacts with PRKCI. Interacts with TELO2 and TTI1. Interacts with RUVBL1 and RUVBL2.
Component of the SMG1C complex composed of SMG1, SMG8 and SMG9; the recruitment of SMG8 to SMG1 N-terminus induces a large conformational change in the SMG1 C-terminal head domain containing the catalytic domain. Component of the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. Interacts with PRKCI. Interacts with TELO2 and TTI1. Interacts with RUVBL1 and RUVBL2. Interacts with UPF2.

Miscellaneous

This gene is located in a region of chromosome 16 that contains 2 segmental duplications. Other genes that are highly related to this exist, but they probably represent pseudogenes.

Similarity

Belongs to the PI3/PI4-kinase family.

Keywords

Acetylation   Alternative splicing   ATP-binding   Complete proteome   Cytoplasm   Direct protein sequencing   DNA damage   DNA repair   Kinase   Manganese   Metal-binding   Nonsense-mediated mRNA decay   Nucleotide-binding   Nucleus   Phosphoprotein   Reference proteome   Transferase   Polymorphism  

Feature

chain  Serine/threonine-protein kinase SMG1
splice variant  In isoform 2.
sequence variant  In dbSNP:rs12051350.

Uniprot

H9JR19   A0A2A4JCX2   A0A2A4JC06   A0A2W1C160   A0A0N1PK13   A0A0L7LLT8   + More
A0A194Q159   A0A2H1VWC4   A0A3S2NCA3   A0A212EJ17   A0A310SKL6   A0A0M9A9D8   A0A067R3Y6   A0A154P395   A0A0L7RD16   A0A087ZS52   A0A2A3EQ41   A0A0J7L5B8   A0A026X4W6   E2C545   A0A2P8YQE7   A0A0C9RCL1   E1ZVC1   A0A3L8DTG4   A0A232F5L9   K7J769   E9IH54   A0A195C7G7   A0A151JAE5   A0A151WSJ2   A0A195FK18   A0A151I5P1   A0A158NTX1   F4WU96   E0VAP7   A0A139WLQ5   A0A0T6B2K1   A0A1B6CNV0   A0A1B6CAC4   A0A1Y1N066   N6UHU4   U4U3T0   T1J1T4   T1I2T7   A0A224XCD0   J9KB53   A0A2H8TIB2   A0A2S2NXY1   A0A1L8EXP0   A0A1L8EQ71   F1PBU5   A0A093I1J3   G3TL75   A0A3Q7Y545   A0A3Q7XMV0   G1TC15   I3MMW0   A0A287CYX9   G1R0C7   A0A384CK10   A0A3Q7XEQ5   M3YPN5   W5PMR8   Q8BKX6   A0A2Y9KTD8   A0A2Y9KFV9   A0A3Q7TUJ0   A0A3Q7SEF6   J9NTC0   A0A2Y9KF75   A0A3Q7ST86   A0A2K5Q9B5   A0A2Y9HQ56   R0JWW4   F6S837   A0A3Q7MW16   A0A0B8RZ42   A0A287BD11   A0A2U3ZQP5   F1MBL6   A0A2K5EVM8   G7NPR4   A0A1U7T5V5   A0A2K5EVM1   A0A2K5EVL0   Q96Q15-2   A0A2U3WMW2   U3IWI0   H0VLT8   A0A2K5Q9U0   K9J0V7   F7F0R6   W5PMR0   A0A287B556   A0A096NRG0   A0A2J8Q5H4   A0A2K5Q9U5   A0A2R9BHL9   Q96Q15-4   G3R3L1  

EC Number

2.7.11.1

Pubmed

19121390    28756777    26354079    26227816    22118469    24845553    + More
24508170    20798317    29403074    30249741    28648823    20075255    21282665    21347285    21719571    20566863    18362917    19820115    28004739    23537049    27762356    16341006    21993624    24813606    20809919    19468303    14621295    16141072    17242355    21183079    23806337    25243066    30723633    19393038    22002653    9566925    11544179    11331269    15175154    15616553    9455477    12168954    8524286    11948212    14636577    12554878    16452507    16964243    17525332    18669648    19413330    19417104    19690332    19608861    20427287    20810650    20371770    20068231    21269460    21245168    21406692    23186163    24275569    17344846    23749191    17495919    22722832   

EMBL

BABH01028615    BABH01028616    BABH01028617    NWSH01001945    PCG69606.1    PCG69605.1    + More
KZ149897    PZC78706.1    KQ459692    KPJ20436.1    JTDY01000627    KOB76417.1    KQ459590    KPI97135.1    ODYU01004851    SOQ45151.1    RSAL01000104    RVE47354.1    AGBW02014545    OWR41458.1    KQ760382    OAD60741.1    KQ435714    KOX79166.1    KK852999    KDR12657.1    KQ434796    KZC05708.1    KQ414615    KOC68709.1    KZ288204    PBC33161.1    LBMM01000708    KMQ97679.1    KK107020    EZA62474.1    GL452737    EFN76953.1    PYGN01000433    PSN46478.1    GBYB01010851    GBYB01010852    GBYB01010853    GBYB01010854    GBYB01010856    JAG80618.1    JAG80619.1    JAG80620.1    JAG80621.1    JAG80623.1    GL434491    EFN74897.1    QOIP01000004    RLU23717.1    NNAY01000979    OXU25637.1    GL763174    EFZ20104.1    KQ978143    KYM96819.1    KQ979275    KYN22035.1    KQ982775    KYQ50767.1    KQ981522    KYN40743.1    KQ976445    KYM86128.1    ADTU01026180    ADTU01026181    GL888355    EGI62204.1    DS235012    EEB10453.1    KQ971319    KYB28836.1    LJIG01016207    KRT81293.1    GEDC01022287    JAS15011.1    GEDC01026897    JAS10401.1    GEZM01019431    JAV89636.1    APGK01019726    KB740120    ENN81315.1    KB631607    ERL84635.1    JH431789    ACPB03006723    ACPB03006724    ACPB03006725    GFTR01008998    JAW07428.1    ABLF02032469    ABLF02032472    GFXV01002049    MBW13854.1    GGMR01009303    MBY21922.1    CM004482    OCT64106.1    CM004483    OCT61503.1    AAEX03004486    AAEX03004487    KL206788    KFV85695.1    AAGW02057093    AGTP01030858    AGTP01030859    AGTP01030860    ADFV01031577    ADFV01031578    ADFV01031579    AEYP01027288    AEYP01027289    AEYP01027290    AEYP01027291    AEYP01027292    AEYP01027293    AMGL01068643    AMGL01068644    AC131788    AC132432    AK129136    AK220328    AK041264    AK047829    AK049391    AK052331    KB743005    EOB02051.1    GAMR01003557    GAMR01003556    JAB30375.1    GBZA01000616    JAG69152.1    AEMK02000017    DQIR01031496    DQIR01166796    DQIR01199951    HCZ86971.1    CM001272    EHH31463.1    AF186377    AB061371    AY014957    AF395444    AC092287    AB007881    U32581    ADON01065093    AAKN02007594    AAKN02007595    GABZ01003339    JAA50186.1    AHZZ02018445    AHZZ02018446    NBAG03000076    PNI91496.1    CABD030099509    CABD030099510    CABD030099511    CABD030099512    CABD030099513    CABD030099514    CABD030099515    CABD030099516    CABD030099517    CABD030099518   

Proteomes

UP000005204    UP000218220    UP000053240    UP000037510    UP000053268    UP000283053    + More
UP000007151    UP000053105    UP000027135    UP000076502    UP000053825    UP000005203    UP000242457    UP000036403    UP000053097    UP000008237    UP000245037    UP000000311    UP000279307    UP000215335    UP000002358    UP000078542    UP000078492    UP000075809    UP000078541    UP000078540    UP000005205    UP000007755    UP000009046    UP000007266    UP000019118    UP000030742    UP000015103    UP000007819    UP000186698    UP000002254    UP000053584    UP000007646    UP000286642    UP000001811    UP000005215    UP000001073    UP000261680    UP000291021    UP000000715    UP000002356    UP000000589    UP000248482    UP000286640    UP000233040    UP000248481    UP000008225    UP000286641    UP000008227    UP000245340    UP000009136    UP000233020    UP000189704    UP000005640    UP000016666    UP000005447    UP000002280    UP000028761    UP000240080    UP000001519   

PRIDE

H9JR19    F1PBU5    Q8BKX6    Q96Q15   

Pfam

PF02260   FATC        + More
PF15785   SMG1
PF00454   PI3_PI4_kinase
PF17229   SMG1_N

Interpro

IPR011009   Kinase-like_dom_sf        + More
IPR003152   FATC_dom       
IPR016024   ARM-type_fold       
IPR036940   PI3/4_kinase_cat_sf       
IPR031559   SMG1       
IPR000403   PI3/4_kinase_cat_dom       
IPR018936   PI3/4_kinase_CS       
IPR039414   SMG1_PIKKc       
IPR014009   PIK_FAT       
IPR035175   SMG1_N       

SUPFAM

SSF56112   SSF56112        + More
SSF48371   SSF48371       

Gene 3D

1.10.1070.11    

CDD

cd05170  PIKKc_SMG1

ProteinModelPortal

H9JR19   A0A2A4JCX2   A0A2A4JC06   A0A2W1C160   A0A0N1PK13   A0A0L7LLT8   + More
A0A194Q159   A0A2H1VWC4   A0A3S2NCA3   A0A212EJ17   A0A310SKL6   A0A0M9A9D8   A0A067R3Y6   A0A154P395   A0A0L7RD16   A0A087ZS52   A0A2A3EQ41   A0A0J7L5B8   A0A026X4W6   E2C545   A0A2P8YQE7   A0A0C9RCL1   E1ZVC1   A0A3L8DTG4   A0A232F5L9   K7J769   E9IH54   A0A195C7G7   A0A151JAE5   A0A151WSJ2   A0A195FK18   A0A151I5P1   A0A158NTX1   F4WU96   E0VAP7   A0A139WLQ5   A0A0T6B2K1   A0A1B6CNV0   A0A1B6CAC4   A0A1Y1N066   N6UHU4   U4U3T0   T1J1T4   T1I2T7   A0A224XCD0   J9KB53   A0A2H8TIB2   A0A2S2NXY1   A0A1L8EXP0   A0A1L8EQ71   F1PBU5   A0A093I1J3   G3TL75   A0A3Q7Y545   A0A3Q7XMV0   G1TC15   I3MMW0   A0A287CYX9   G1R0C7   A0A384CK10   A0A3Q7XEQ5   M3YPN5   W5PMR8   Q8BKX6   A0A2Y9KTD8   A0A2Y9KFV9   A0A3Q7TUJ0   A0A3Q7SEF6   J9NTC0   A0A2Y9KF75   A0A3Q7ST86   A0A2K5Q9B5   A0A2Y9HQ56   R0JWW4   F6S837   A0A3Q7MW16   A0A0B8RZ42   A0A287BD11   A0A2U3ZQP5   F1MBL6   A0A2K5EVM8   G7NPR4   A0A1U7T5V5   A0A2K5EVM1   A0A2K5EVL0   Q96Q15-2   A0A2U3WMW2   U3IWI0   H0VLT8   A0A2K5Q9U0   K9J0V7   F7F0R6   W5PMR0   A0A287B556   A0A096NRG0   A0A2J8Q5H4   A0A2K5Q9U5   A0A2R9BHL9   Q96Q15-4   G3R3L1  

KEGG

101739213    K08873 serine/threonine-protein kinase SMG1 [EC:2.7.11.1] | (RefSeq) serine/threonine-protein kinase

PATHWAY

03015    mRNA surveillance pathway - Bombyx mori (domestic silkworm)

GO

GO:0016301   GO:0005524   GO:0000184   GO:0004674   GO:0005634   GO:0046854   GO:0005737   GO:0042162   GO:0018105   GO:2001020   GO:0046777   GO:0005654   GO:0032204   GO:0016021   GO:0006281   GO:0046872   GO:0005829   GO:0004672   GO:0003723   GO:0006406   GO:0008270   GO:0003824   GO:0009982   GO:0006744   GO:0016627   GO:0030145   GO:0015923   GO:0006260  

Subcellular location

Nucleus  
Cytoplasm  

Length:

618

Number of predicted TMHs:

1

Exp number of AAs in TMHs:

19.01563

Exp number, first 60 AAs:

0.03413

Total prob of N-in:

0.00315

outside

1  -  597

TMhelix

598  -  617

inside

618  -  618

 

 

Pi

244.206059

Theta

175.126662

Tajima's D

1.044368

CLR

0.083448

CSRT

0.67591620418979

Interpretation

Uncertain