Gene

KWMTBOMO06318  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA011891

Annotation

PREDICTED:_adenylyltransferase_and_sulfurtransferase_MOCS3_isoform_X1_[Papilio_xuthus]

Location

Bomo_Chr11(+):4697563-4711817      View in EpiBrowser

Full name

Adenylyltransferase and sulfurtransferase MOCS3 homolog       + More
Adenylyltransferase and sulfurtransferase MOCS3      
Adenylyltransferase and sulfurtransferase UBA4      

Alternative Name

UBA4 homolog
Ubiquitin-like protein activator 4 homolog
Molybdenum cofactor synthesis protein 3
Ubiquitin activating enzyme 4
Ubiquitin-like protein activator 4

Location in the cell

Cytoplasmic   Reliability : 1.928 Nuclear   Reliability : 1.424

CDS

ATGGACAGAGTCAGTGTTTTAGAGACGGAAATATCAGAACTTCGCAGAATTTTAAAGGAAAAGGAAAATGAACTTCAGGAATTAAAAAGTTTGATTAGTGATCAACAATCAAGTAAAGAATTAAATAATCACAGTCAGCCGATAAGTGATGCTTCAAAATCATCAGTAGATGCCTGTCTTCCAAAATGGGCGATAGAAAGGTACAGTCGTCAGATCCTTCTCTCGGACATCGGCGTCGAAGGACAGGTTAAGATCTGTTCTGCCAAAGTACTGATTGTGGGAGCCGGCGGCCTGGGATGTCCGGCTGCCATGTACCTTGCCGGGGCGGGCATTGGCGAAATAGGCATCGTGGATTATGACGCGGTGGATCTGACCAACGTGCACAGACAACTGCTGCACCATGAAAGCAACGAGAACACCAGCAAGGCGTTCTCGGCCGCTGAGTCTCTGAGATGTATAAACTCGAAAATTAAAATCACACCATACAACGTTCAGTTGGACTCGAAGAACGCTCAGCAAATCGCTTCAGCTTATGACTTGGTGCTCGACTGCAGCGACAACGTCCCGACCAGGTATCTGTTGAACGACCTGTGCGTTATGCTAAAGATCCCTCTGATATCGGGAAGCGCTTTGAAAATGGAGGGGCAGCTCACGATATACGGGTACAGGAGACAGGACCATCACCAGGAGACGTACATAGGGCCTTGCTACCGCTGCGTGTTCCCCACGCCCCCCCCTCCCGCTGCGGTGGGCAGCTGCTCCGCCCACGGGGTGGCCGGGCCCGTCCCGGGGACCGTGGGAACCCTGCAGGCCCTGGAGGCGATAAAGTACGTGATAGGCAGGACCGAGAGGCACCTGCTGGTCGGCCGCATGCTGCTGTTCGACGGAGAGGACGCGACCTTTAGGGCAGTAAAGTTGCGTCCGAGGAGACCCGCGTGCGTGGCGTGTGGGGACCGCGGAGGGGGCCGCCTGATCGATTATGAGCAGTTCTGCAATGCGCCGGCCAAAGAGAAGGACTTGGATCTGGAGGTGCTGCCGCCGGAAAATCGGGTCAGCGCCAACGAACTGAACGTAATGATGAATAACGAGGAGCGAGGAGGACGTCATCTCGTGGTGGACGTTAGGAACGAGCAGGAGTACGCGATGTGCCACATTCAGGGCTCGGTCAACTACCCCGTGGAGAACTTGCACCGGAACTTGGAGGAGTTGATCGAGAAGACCAAGGAGTTTGATGGAGGTGAGCTTTTTTTTATTGCTTAA

Protein

MDRVSVLETEISELRRILKEKENELQELKSLISDQQSSKELNNHSQPISDASKSSVDACLPKWAIERYSRQILLSDIGVEGQVKICSAKVLIVGAGGLGCPAAMYLAGAGIGEIGIVDYDAVDLTNVHRQLLHHESNENTSKAFSAAESLRCINSKIKITPYNVQLDSKNAQQIASAYDLVLDCSDNVPTRYLLNDLCVMLKIPLISGSALKMEGQLTIYGYRRQDHHQETYIGPCYRCVFPTPPPPAAVGSCSAHGVAGPVPGTVGTLQALEAIKYVIGRTERHLLVGRMLLFDGEDATFRAVKLRPRRPACVACGDRGGGRLIDYEQFCNAPAKEKDLDLEVLPPENRVSANELNVMMNNEERGGRHLVVDVRNEQEYAMCHIQGSVNYPVENLHRNLEELIEKTKEFDGGELFFIA

Description

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Acts by mediating the C-terminal thiocarboxylation of the sulfur carrier URM1. Its N-terminus first activates URM1 as acyl-adenylate (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 to form thiocarboxylation (-COSH) of its C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Acts by mediating the C-terminal thiocarboxylation of sulfur carrier URM1. Its N-terminus first activates URM1 as acyl-adenylate (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 to form thiocarboxylation (-COSH) of its C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.

Catalytic Activity

[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + ATP + H(+) = [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + diphosphate
[molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly-AMP + AH2 + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] = [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H(+) + L-cysteinyl-[cysteine desulfurase]

Cofactor

Zn(2+)

Similarity

In the N-terminal section; belongs to the HesA/MoeB/ThiF family. UBA4 subfamily.

Keywords

ATP-binding   Complete proteome   Cytoplasm   Metal-binding   Molybdenum cofactor biosynthesis   Multifunctional enzyme   Nucleotide-binding   Reference proteome   Transferase   tRNA processing   Zinc  

Feature

chain  Adenylyltransferase and sulfurtransferase MOCS3

Uniprot

H9JQT5   A0A3S2NXN3   A0A2A4K967   A0A2H1W8W7   A0A182FPX2   U5EW75   + More
B0W377   A0A2W1BD56   A0A182Y3U8   A0A182RSX2   A0A182M1E2   A0A182GLG4   A0A182H1U8   A0A1Y9IVZ6   A0A023EUI2   A0A1I8JTE7   Q7PY41   A0A182NSV6   A0A182PSH0   A0A0L7RC21   A0A182INL1   A0A1I8JVV7   A0A182JW94   A0A182U995   A0A1Q3F9S4   Q17CA7   A0A2A3E7A6   A0A154PI34   A0A2K7P699   A0A182V2V2   A0A084VZE7   A0A087ZMT7   K7J5G0   A0A158NJ76   A0A195BKU7   A0A232FKV3   A0A2M4AHX4   A0A210PXI6   A0A336M9W7   A0A2M4BQS2   A0A1B6LZH7   A0A2M4BQH9   A0A0N8A194   A0A0P5BSM7   A0A0N8BGR9   W5JI26   A0A0P5XJ57   E2AJ64   A0A0N8B6W2   A0A0P5NXT0   A0A0P6FD10   A0A1B6GAM7   A0A0P5SGE4   A0A0P4WQY5   A0A0N7ZTT7   A0A3B5BGN4   A0A0P5QWH9   A0A026WWM2   A0A164L0V4   A0A0P6ACZ8   A0A0P5I8L0   A0A0P5IUT9   A0A0P6IFC5   A0A1S3J2P2   A0A0P5W462   A0A182QNA0   A0A3B3C7A8   F4WKP0   A0A151JV91   A0A151WE67   A0A0B6ZFS4   A0A1B6IGC9   A0A151J922   A0A0K8S6F3   A0A0P4Z5M7   A0A2C9LM44   A0A151I7G9   V3ZM40   B4LRB9   A0A0A9YR34   A0A0P4VQ85   T1HJ70   A0A146L9V2   A0A1B0AKS4   A0A3Q1EXR4   A0A3B0JXX0   G3NV70   A0A1A9XUH2   E5SIM0   A0A310SGL4   A0A0V0VQR2   A0A0V1PBK0   A0A3P8UHD4   A0A0V0TYT8   A0A1Y3E887   G3NV64   A0A3P8UIC5   A0A177WB71   F4NU54  

Pubmed

19121390    28756777    25244985    26483478    24945155    12364791    + More
17510324    24438588    20075255    21347285    28648823    28812685    20920257    23761445    20798317    24508170    30249741    29451363    21719571    15562597    23254933    17994087    25401762    27129103    26823975    24487278   

EMBL

BABH01028732    BABH01028733    BABH01028734    RSAL01000109    RVE47171.1    NWSH01000042    + More
PCG80333.1    ODYU01007055    SOQ49487.1    GANO01001549    JAB58322.1    DS231831    KZ150447    PZC70840.1    AXCM01000028    JXUM01071933    KQ562693    KXJ75321.1    JXUM01023254    JXUM01023255    KQ560656    KXJ81407.1    GAPW01001479    JAC12119.1    APCN01000910    AAAB01008987    KQ414617    KOC68384.1    GFDL01010749    JAV24296.1    CH477310    KZ288363    PBC26921.1    KQ434899    KZC10860.1    ATLV01018748    KE525248    KFB43341.1    ADTU01017554    KQ976453    KYM85394.1    NNAY01000065    OXU31364.1    GGFK01006887    MBW40208.1    NEDP02005416    OWF41195.1    UFQT01000609    SSX25689.1    GGFJ01006130    MBW55271.1    GEBQ01010932    JAT29045.1    GGFJ01006131    MBW55272.1    GDIP01192385    JAJ31017.1    GDIP01195434    JAJ27968.1    GDIQ01179307    GDIQ01177866    JAK72418.1    ADMH02001345    ETN62938.1    GDIP01083602    JAM20113.1    GL439967    EFN66504.1    GDIQ01206963    JAK44762.1    GDIQ01143271    JAL08455.1    GDIQ01065566    JAN29171.1    GECZ01010271    JAS59498.1    GDIP01140046    JAL63668.1    GDIP01254272    JAI69129.1    GDIP01213241    JAJ10161.1    GDIQ01115388    JAL36338.1    KK107078    QOIP01000011    EZA60457.1    RLU16629.1    LRGB01003216    KZS03699.1    GDIP01031005    JAM72710.1    GDIQ01217302    JAK34423.1    GDIQ01216308    JAK35417.1    GDIQ01006889    JAN87848.1    GDIP01091299    JAM12416.1    AXCN02001149    GL888206    EGI65132.1    KQ981703    KYN37443.1    KQ983247    KYQ46164.1    HACG01019831    CEK66696.1    GECU01021730    JAS85976.1    KQ979480    KYN21255.1    GBRD01017083    JAG48744.1    GDIP01221947    JAJ01455.1    KQ978411    KYM94096.1    KB203660    ESO83505.1    CH940649    GBHO01008965    JAG34639.1    GDKW01002604    JAI53991.1    ACPB03021235    GDHC01015219    JAQ03410.1    JXJN01027914    OUUW01000010    SPP85913.1    JYDH01000114    KRY31641.1    KQ764145    OAD54590.1    JYDN01000016    KRX65501.1    JYDM01000024    KRZ93612.1    JYDJ01000102    KRX44186.1    LVZM01021474    OUC41342.1    DS022300    OAJ37357.1    GL882879    EGF84382.1   

Proteomes

UP000005204    UP000283053    UP000218220    UP000069272    UP000002320    UP000076408    + More
UP000075900    UP000075883    UP000069940    UP000249989    UP000075920    UP000075840    UP000007062    UP000075884    UP000075885    UP000053825    UP000075880    UP000076407    UP000075881    UP000075902    UP000008820    UP000242457    UP000076502    UP000075903    UP000030765    UP000005203    UP000002358    UP000005205    UP000078540    UP000215335    UP000242188    UP000000673    UP000000311    UP000261400    UP000053097    UP000279307    UP000076858    UP000085678    UP000075886    UP000261560    UP000007755    UP000078541    UP000075809    UP000078492    UP000076420    UP000078542    UP000030746    UP000008792    UP000015103    UP000092460    UP000257200    UP000268350    UP000007635    UP000092443    UP000054776    UP000054681    UP000054924    UP000265120    UP000055048    UP000243006    UP000077115    UP000007241   

PRIDE

H9JQT5    B0W377    Q7PY41    Q17CA7    B4LRB9   

Pfam

PF00581   Rhodanese        + More
PF00899   ThiF

Interpro

IPR036873   Rhodanese-like_dom_sf        + More
IPR035985   Ubiquitin-activating_enz       
IPR000594   ThiF_NAD_FAD-bd       
IPR028885   MOCS3/Uba4       
IPR001763   Rhodanese-like_dom       

SUPFAM

SSF69572   SSF69572        + More
SSF52821   SSF52821       

Gene 3D

3.40.250.10    

ProteinModelPortal

H9JQT5   A0A3S2NXN3   A0A2A4K967   A0A2H1W8W7   A0A182FPX2   U5EW75   + More
B0W377   A0A2W1BD56   A0A182Y3U8   A0A182RSX2   A0A182M1E2   A0A182GLG4   A0A182H1U8   A0A1Y9IVZ6   A0A023EUI2   A0A1I8JTE7   Q7PY41   A0A182NSV6   A0A182PSH0   A0A0L7RC21   A0A182INL1   A0A1I8JVV7   A0A182JW94   A0A182U995   A0A1Q3F9S4   Q17CA7   A0A2A3E7A6   A0A154PI34   A0A2K7P699   A0A182V2V2   A0A084VZE7   A0A087ZMT7   K7J5G0   A0A158NJ76   A0A195BKU7   A0A232FKV3   A0A2M4AHX4   A0A210PXI6   A0A336M9W7   A0A2M4BQS2   A0A1B6LZH7   A0A2M4BQH9   A0A0N8A194   A0A0P5BSM7   A0A0N8BGR9   W5JI26   A0A0P5XJ57   E2AJ64   A0A0N8B6W2   A0A0P5NXT0   A0A0P6FD10   A0A1B6GAM7   A0A0P5SGE4   A0A0P4WQY5   A0A0N7ZTT7   A0A3B5BGN4   A0A0P5QWH9   A0A026WWM2   A0A164L0V4   A0A0P6ACZ8   A0A0P5I8L0   A0A0P5IUT9   A0A0P6IFC5   A0A1S3J2P2   A0A0P5W462   A0A182QNA0   A0A3B3C7A8   F4WKP0   A0A151JV91   A0A151WE67   A0A0B6ZFS4   A0A1B6IGC9   A0A151J922   A0A0K8S6F3   A0A0P4Z5M7   A0A2C9LM44   A0A151I7G9   V3ZM40   B4LRB9   A0A0A9YR34   A0A0P4VQ85   T1HJ70   A0A146L9V2   A0A1B0AKS4   A0A3Q1EXR4   A0A3B0JXX0   G3NV70   A0A1A9XUH2   E5SIM0   A0A310SGL4   A0A0V0VQR2   A0A0V1PBK0   A0A3P8UHD4   A0A0V0TYT8   A0A1Y3E887   G3NV64   A0A3P8UIC5   A0A177WB71   F4NU54  

PDB

1JWB     E-value=4.57211e-43,     Score=440

KEGG

101737191    K11996 adenylyltransferase and sulfurtransferase [EC:2.7.7.80 2.8.1.11] | (RefSeq) adenylyltransfera

PATHWAY

04122    Sulfur relay system - Bombyx mori (domestic silkworm)

GO

GO:0005524   GO:0006777   GO:0018192   GO:0008641   GO:0046872   GO:0005829   GO:0002143   GO:0004792   GO:0016779   GO:0034227   GO:0016783   GO:0002098   GO:0005737   GO:0016740   GO:0061604   GO:0061605   GO:0042292   GO:0032447   GO:0016021   GO:0032324   GO:0019008   GO:0051015   GO:0045296   GO:0016020   GO:0016627   GO:0030145   GO:0015923   GO:0005634   GO:0006260  

Subcellular location

Cytoplasm  

Length:

419

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

7.13122

Exp number, first 60 AAs:

0

Total prob of N-in:

0.32372

outside

1  -  419

 

 

Pi

251.413426

Theta

173.40865

Tajima's D

1.188086

CLR

0.112817

CSRT

0.708964551772411

Interpretation

Uncertain