Gene

KWMTBOMO06231  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA001801

Annotation

PREDICTED:_ruvB-like_2_[Papilio_polytes]

Location

Bomo_Chr11(+):3032255-3039289      View in EpiBrowser

Full name

RuvB-like helicase       + More
RuvB-like 2      

Alternative Name

Reptin
p47 protein

Location in the cell

Mitochondrial   Reliability : 2.129

CDS

ATGGCTTCAATAGCAGCGGCGCAAGTACAAGAAGTGCGTTCTATAACGCGCATCGAGAGAATTGGCGCGCATTCCCACATTAGAGGACTGGGCTTAGATGACTCTTTGGAGCCAAGACAAGTATCGCAGGGCATGGTCGGCCAGAAGATGGCAAGGAAGGCCGCCGGAGTCATCTTACAGATGATTCGAGAAGGTAAGATAGCGGGACGAGCAGTGTTATTGGCCGGTCAGCCTGGTACAGGGAAGACTGCCATCGCTATGGGACTAGCACAAGCTCTGGGACCGGACACACCATTTACGAGCATGGCCGGTTCGGAGATATTCTCTTTGGAAATGAGTAAAACTGAGGCTCTAACGCAGGCCATCAGGAAGTCTATAGGTATTAGAATTAAGGAGGAGTCTGAGATAATTGAAGGGGAGGTCGTCGAGGTGGTAGTGGAGCGTGCGGCGGGGGGCGGCGGCGCCAGGGTCGGCCGTCTCACCCTCAAGACCACCGACATGGAAACGAACTACGACATGGGAGCCAAAATGATTGATTCGCTGCTTAAAGAGAAGGTTCAAGCCGGAGATGTGATCACCATAGACAAGGCTACAGGCAAAATAAACAAGCTCGGCAGGAGTTTTGCCAGAGCCAGAGACTATGATGCTACCGGTCAGCAGGCCAGGTTCGTGCAGTGCCCCGAGGGCGAGCTGCAGAAGCGCAAGGAGGTGGTGCACACGGTGACGCTGCACGAGGTGGACGTCATCAACTCCAGGACCCACGGCTTCCTGGCACTGTTCTCCGGCGACACGGGCGAGATTAAATCTGAGATTCGGGAGCAGATTAACAGTAAGGTGGCTGAGTGGAGAGAGGAGGGGAAGGCAGAAATGATTCCGGGGGTGCTATTCATCGATGAGGCTCACATGCTGGACATTGAGTGCTTCTCGTTCCTCAATCGCGCGCTGGAATCGGAAACCGCTCCCATCGTGATCATGGCGACGAATCGAGGGATCACCCGGATACGGGGTACCACTTACCGCAGCCCGCACGGCATACCGCTGGACCTGCTCGACCGGATGATCATCGTGGCCACCTCGCCGTACTCCCAGAGTGAGCTCAAGGACATACTCAATATACGGTGA

Protein

MASIAAAQVQEVRSITRIERIGAHSHIRGLGLDDSLEPRQVSQGMVGQKMARKAAGVILQMIREGKIAGRAVLLAGQPGTGKTAIAMGLAQALGPDTPFTSMAGSEIFSLEMSKTEALTQAIRKSIGIRIKEESEIIEGEVVEVVVERAAGGGGARVGRLTLKTTDMETNYDMGAKMIDSLLKEKVQAGDVITIDKATGKINKLGRSFARARDYDATGQQARFVQCPEGELQKRKEVVHTVTLHEVDVINSRTHGFLALFSGDTGEIKSEIREQINSKVAEWREEGKAEMIPGVLFIDEAHMLDIECFSFLNRALESETAPIVIMATNRGITRIRGTTYRSPHGIPLDLLDRMIIVATSPYSQSELKDILNIR

Description

Proposed core component of the chromatin remodeling Ino80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.
Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.
Has single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity suggesting a role in nuclear processes such as recombination and transcription.
Involved in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway where it negatively regulates expression of ER stress response genes.
Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity.
Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome (By similarity).
Plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2 (By similarity).

Catalytic Activity

ATP + H2O = ADP + H(+) + phosphate

Subunit

Forms homohexameric rings (Probable). Can form a dodecamer with ruvbl2 made of two stacked hexameric rings. Is a component of the RNA polymerase II holoenzyme complex. Component of the chromatin-remodeling Ino80 complex. Component of some MLL1/MLL complex (By similarity).
Forms homohexameric rings (Probable). Can form a dodecamer with RUVBL1 made of two stacked hexameric rings; however, even though RUVBL1 and RUVBL2 are present in equimolar ratio, the oligomeric status of each hexamer is not known. Oligomerization may regulate binding to nucleic acids and conversely, binding to nucleic acids may affect the dodecameric assembly. Interacts with the transcriptional activation domain of MYC. Interacts With ATF2. Component of the RNA polymerase II holoenzyme complex. May also act to bridge the LEF1/TCF1-CTNNB1 complex and TBP. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. The NuA4 complex interacts with MYC and the adenovirus E1A protein. RUVBL2 interacts with EP400. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41. Interacts with NPAT. Component of the chromatin-remodeling INO80 complex; specifically part of a complex module associated with the helicase ATP-binding and the helicase C-terminal domain of INO80. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with IGHMBP2. Interacts with TELO2. Interacts with HINT1. Component of a SWR1-like complex. Component of the R2TP complex composed at least of PIHD1, RUVBL1, RUVBL2 and RPAP3. Interacts with ITFG1. Interacts with ZMYND10. Interacts with WAC; WAC positively regulates MTOR activity by promoting the assembly of the TTT complex composed of TELO2, TTI1 and TTI2 and the RUVBL complex composed of RUVBL1 and RUVBL2 into the TTT-RUVBL complex which leads to the dimerization of the mTORC1 complex and its subsequent activation. Forms a complex with APPL1 and APPL2 (By similarity).

Similarity

Belongs to the RuvB family.

Keywords

ATP-binding   DNA damage   DNA recombination   DNA repair   Helicase   Hydrolase   Nucleotide-binding   Nucleus   Transcription   Transcription regulation   Acetylation   Activator   Chromatin regulator   Complete proteome   Isopeptide bond   Phosphoprotein   Reference proteome   Ubl conjugation  

Feature

chain  RuvB-like helicase

Uniprot

A0A2A4K490   A0A0N0PC54   A0A2W1BK50   A0A1B6GNH1   A0A1B6IPC2   A0A2A3ELN2   + More
A0A088ARN2   A0A0L7RDH9   A0A0M8ZWR1   A0A1B6D3M9   A0A154PA15   A0A0C9QK39   A0A310SDU0   E2B6H7   A0A2J7RI53   A0A151IGB9   E9IXY8   A0A067QZM3   A0A195DCE3   A0A0J7K820   E0VPV9   A0A158NEA8   A0A2J7RI55   A0A151WFD9   A0A0J7KGE9   F4WM57   A0A232FD79   K7IZH3   A0A026WXH9   E9GRY1   A0A195FUP0   A0A2R5LM78   G1KMP5   Q0IH85   A0A0P5UL26   A0A0B8RWC7   G3MNC5   A0A0P5DJ95   A0A0P5UNC6   A0A0P5NVU9   A0A0P5ULG6   Q801R1   A0A1W7RDN6   T1DL73   A0A0F7Z5R1   A0A0P5AGE5   Q9DE27   A0A0P6H0Y4   A0A0P5YYQ6   A0A0N8DWN2   Q28GB7   A0A224YYM6   A0A131YT57   L7M846   A0A0P5HQ08   A0A0F7YZJ1   A0A2H6NAJ8   A0A1S3JEQ9   A0A131XH09   A0A0P6F024   A0A0P4ZBA1   C3Y6A4   A0A2K5QG80   A0A2D4NPV6   A0A0P5JVV9   A0A0P5BH73   A0A2K5EYP0   A0A0P5QCE6   A0A2K5EYU9   F6UB78   A0A2K6U4D8   F7GQG2   A0A151NKW1   A0A286XN46   A0A061I456   A0A287D5H8   A0A1U7RDA2   G3V8T5   Q3TXT7   F6X277   A0A2Y9F1Z3   A0A1S3JER7   Q9WTM5   V9KTC5   A0A1S3G4W2   Q4QQS4   A0A2Y9F0F3   A0A1A7YLD1   I3MM17   L5LT14   A0A3Q2D7M0   A0A384BKV9   A0A3Q0DWP0   A0A3Q7TNI3   A0A0P5S644   A0A146WVK1   A0A3Q2U7I7   G1QY99   A0A146WSZ4   H0W8I2  

EC Number

3.6.4.12

Pubmed

26354079    28756777    20798317    21282665    24845553    20566863    + More
21347285    21719571    28648823    20075255    24508170    30249741    21292972    27762356    25476704    22216098    26358130    23758969    10842076    20431018    28797301    26830274    25576852    28049606    18563158    17495919    25243066    22293439    21993624    23929341    15057822    15632090    10349636    11042159    11076861    11217851    12466851    12040188    15489334    16141073    21183079    19892987    10220587    24402279    24813606    15592404   

EMBL

NWSH01000147    PCG79067.1    KQ460772    KPJ12452.1    KZ149995    PZC75452.1    + More
GECZ01005804    JAS63965.1    GECU01018924    JAS88782.1    KZ288222    PBC32106.1    KQ414614    KOC68864.1    KQ435814    KOX72680.1    GEDC01017004    GEDC01007112    JAS20294.1    JAS30186.1    KQ434856    KZC08735.1    GBYB01000972    JAG70739.1    KQ760869    OAD58769.1    GL445973    EFN88721.1    NEVH01003505    PNF40512.1    KQ977726    KYN00309.1    GL766836    EFZ14571.1    KK852809    KDR16004.1    KQ980989    KYN10580.1    LBMM01012069    KMQ86429.1    DS235379    EEB15415.1    ADTU01001328    PNF40514.1    KQ983219    KYQ46550.1    LBMM01007939    KMQ89281.1    GL888217    EGI64701.1    NNAY01000430    OXU28453.1    KK107069    QOIP01000002    EZA60732.1    RLU25975.1    GL732561    EFX77610.1    KQ981264    KYN44007.1    GGLE01006496    MBY10622.1    BC123265    CM004478    AAI23266.1    OCT73146.1    GDIP01111401    JAL92313.1    GBSH01002404    JAG66622.1    JO843376    AEO34993.1    GDIP01161390    JAJ62012.1    GDIP01112601    JAL91113.1    GDIQ01144085    JAL07641.1    GDIP01111400    JAL92314.1    BC047966    AAH47966.1    GDAY02002171    JAV49261.1    GAAZ01002212    JAA95731.1    GBEX01003007    JAI11553.1    GDIP01202286    JAJ21116.1    AF218071    GDIQ01026112    JAN68625.1    GDIP01051412    JAM52303.1    GDIP01073002    GDIQ01084612    JAM30713.1    JAN10125.1    AAMC01090096    CR761457    CAJ83917.1    GFPF01011560    MAA22706.1    GEDV01006887    JAP81670.1    GACK01004834    JAA60200.1    GDIQ01224698    JAK27027.1    GBEW01000909    JAI09456.1    IACI01072922    LAA28282.1    GEFH01003580    JAP65001.1    GDIQ01063304    JAN31433.1    GDIP01216533    JAJ06869.1    GG666487    EEN64501.1    IACN01014288    LAB47740.1    GDIQ01194452    JAK57273.1    GDIP01189983    JAJ33419.1    GDIQ01116298    JAL35428.1    GAMT01008890    GAMS01009298    GAMR01004745    GAMQ01005695    GAMP01009703    JAB02971.1    JAB13838.1    JAB29187.1    JAB36156.1    JAB43052.1    AKHW03002822    KYO37125.1    AAKN02046418    AAKN02046419    KE680915    ERE71143.1    AGTP01089690    AC128792    CH473979    EDM07354.1    BC115809    BC116693    AK144156    AK159112    CH466603    AAI15810.1    BAE34828.1    EDL22854.1    AB013912    JW868924    AFP01442.1    BC098042    AAH98042.1    HADW01019644    HADX01009200    SBP31432.1    KB109009    ELK28598.1    GDIQ01108990    JAL42736.1    GCES01053286    JAR33037.1    AADN05000747    ADFV01051887    ADFV01051888    ADFV01051889    ADFV01051890    ADFV01051891    GCES01053283    JAR33040.1   

Proteomes

UP000218220    UP000053240    UP000242457    UP000005203    UP000053825    UP000053105    + More
UP000076502    UP000008237    UP000235965    UP000078542    UP000027135    UP000078492    UP000036403    UP000009046    UP000005205    UP000075809    UP000007755    UP000215335    UP000002358    UP000053097    UP000279307    UP000000305    UP000078541    UP000001646    UP000186698    UP000008143    UP000085678    UP000001554    UP000233040    UP000233020    UP000002280    UP000233220    UP000050525    UP000005447    UP000030759    UP000005215    UP000189706    UP000002494    UP000002281    UP000248484    UP000000589    UP000081671    UP000265020    UP000261680    UP000291021    UP000189704    UP000286642    UP000000539    UP000001073   

PRIDE

G3MNC5    Q9DE27    L7M846    F6X277    Q9WTM5    Q4QQS4    + More

Pfam

PF06068   TIP49        + More
PF17856   TIP49_C

Interpro

IPR041048   RuvB-like_C        + More
IPR003593   AAA+_ATPase       
IPR027238   RuvB-like       
IPR010339   TIP49_P-loop       
IPR037942   RUVBL2       
IPR027417   P-loop_NTPase       

SUPFAM

SSF52540   SSF52540       

ProteinModelPortal

A0A2A4K490   A0A0N0PC54   A0A2W1BK50   A0A1B6GNH1   A0A1B6IPC2   A0A2A3ELN2   + More
A0A088ARN2   A0A0L7RDH9   A0A0M8ZWR1   A0A1B6D3M9   A0A154PA15   A0A0C9QK39   A0A310SDU0   E2B6H7   A0A2J7RI53   A0A151IGB9   E9IXY8   A0A067QZM3   A0A195DCE3   A0A0J7K820   E0VPV9   A0A158NEA8   A0A2J7RI55   A0A151WFD9   A0A0J7KGE9   F4WM57   A0A232FD79   K7IZH3   A0A026WXH9   E9GRY1   A0A195FUP0   A0A2R5LM78   G1KMP5   Q0IH85   A0A0P5UL26   A0A0B8RWC7   G3MNC5   A0A0P5DJ95   A0A0P5UNC6   A0A0P5NVU9   A0A0P5ULG6   Q801R1   A0A1W7RDN6   T1DL73   A0A0F7Z5R1   A0A0P5AGE5   Q9DE27   A0A0P6H0Y4   A0A0P5YYQ6   A0A0N8DWN2   Q28GB7   A0A224YYM6   A0A131YT57   L7M846   A0A0P5HQ08   A0A0F7YZJ1   A0A2H6NAJ8   A0A1S3JEQ9   A0A131XH09   A0A0P6F024   A0A0P4ZBA1   C3Y6A4   A0A2K5QG80   A0A2D4NPV6   A0A0P5JVV9   A0A0P5BH73   A0A2K5EYP0   A0A0P5QCE6   A0A2K5EYU9   F6UB78   A0A2K6U4D8   F7GQG2   A0A151NKW1   A0A286XN46   A0A061I456   A0A287D5H8   A0A1U7RDA2   G3V8T5   Q3TXT7   F6X277   A0A2Y9F1Z3   A0A1S3JER7   Q9WTM5   V9KTC5   A0A1S3G4W2   Q4QQS4   A0A2Y9F0F3   A0A1A7YLD1   I3MM17   L5LT14   A0A3Q2D7M0   A0A384BKV9   A0A3Q0DWP0   A0A3Q7TNI3   A0A0P5S644   A0A146WVK1   A0A3Q2U7I7   G1QY99   A0A146WSZ4   H0W8I2  

PDB

6QI9     E-value=1.29105e-143,     Score=1306

KEGG

101741791    K11338 RuvB-like protein 2 [EC:3.6.4.12] | (RefSeq) ruvB-like 2

GO

GO:0043141   GO:0097255   GO:0035267   GO:0006281   GO:0005524   GO:0006325   GO:0031011   GO:0000812   GO:0006357   GO:0006338   GO:0004003   GO:0016573   GO:0000492   GO:0005829   GO:0008013   GO:0071733   GO:0000980   GO:0043967   GO:0071169   GO:0071339   GO:0090090   GO:0005719   GO:0071899   GO:0000979   GO:0031490   GO:0017025   GO:0042802   GO:0035066   GO:0034644   GO:0003714   GO:0005813   GO:0006310   GO:0071392   GO:0043968   GO:0044458   GO:0003351   GO:0048565   GO:0045892   GO:0007507   GO:0060420   GO:0070286   GO:0051117   GO:0043531   GO:0001094   GO:0042803   GO:1990904   GO:0045944   GO:0005737   GO:0005654   GO:0005634   GO:0003678   GO:0004386   GO:0006412   GO:0016020   GO:0022857   GO:0004559   GO:0006013   GO:0015923   GO:0006260  

PANTHER

PTHR11093:SF2   PTHR11093  

Subcellular location

Nucleus  

Length:

373

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.25202

Exp number, first 60 AAs:

0.00048

Total prob of N-in:

0.03175

outside

1  -  373

 

 

Pi

274.824049

Theta

187.444091

Tajima's D

1.570267

CLR

0.236031

CSRT

0.808559572021399

Interpretation

Uncertain