Gene

KWMTBOMO06182  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA001773

Annotation

PREDICTED:_protein_prune_homolog_isoform_X2_[Bombyx_mori]

Location

Bomo_Chr11(+):1971591-1976586      View in EpiBrowser

Full name

Exopolyphosphatase PRUNE1      

Alternative Name

PRUNEM1

Location in the cell

Cytoplasmic   Reliability : 1.684 Nuclear   Reliability : 1.629

CDS

ATGAAAGAATTTTTAAACAACATTGTAAATAAGTTGAAATCCAATGATTATGACAACATTAATATCGTTATTGGCAACGAAAGCTGCGATCTAGATTCAGCGGTTTGCTCCATTGTCTACGCTCTATATTTAAATTGGCAACACAATCAGATAAAATGCAAGGTATGCACGAAAGACAAACGTGGTGCTTCATCCAAAGACGATATATTCATCCCGATATTAAACATGGTCAGACAGGATTTTGCTCTCAAAACAGAGGTCATGTATCTTTTCAATAAACTGGGAATCACAGAAGGTGATTTAATTTTTAGAAACGATTATGATTTGTGCAGTTTGCTACGAGATTCTAAGTGCAACGTGGTTTTGGTAGATCATCATGTACTGGCTGCTAATGACGTTTTTCTGTCAGCCTTTGTCACCGAGATCATTGATCACAGACCACTAGATAAATCAAGGTGGACCTACAAAGGCGATACAAGATTAATCATTGAGATAGTCGGTTCATGCGCTACCCTTGTAACACGTCGAATCAAAGAGATGTGCTTACTATTAGGAAAGGAATTCCAGTTCTTCAAAGACCACATGCCCTGTAGTCAAATGCTGTATTCAACAATCATCCTAGACACGGTGAATTTCTCGAAAGAATTCAATAAGGGAACACCAGAGGACGAGGAAATGATTGACTTGCTGGAGCGAGTTTTGATGATTGAAAATCCCATTGACGAAAGGCAACGGGTATTGGATGAGCTGACAAAGGCCAAAAGTGACGTATCGAAATTAACGGCGTCACAATTGCTCAGGAAAGACGTGAAAATCGTCGAAGACGTATTAATACCCAGCTTCCCGATGTTAGTGGAGGAGTTCCTACGGCTGGATGATGCTGTTGACGCTGTAAAGGAAGTCTTGAGCCAGCGCGAGTGTTTAGTAGCTTTGCTGCTAGGAATGGATCTGACAAGTGGAATGAAGAGAGACATGGCGGTGATGTCCCCGAACAATGAGAATCTGGCCGAAAAGCTCGATTCGTTTCTCGTTGAATGGTCGTCGCCGTCGTTCGAGCTGCGCCCGCGTGACGGTGCGGTCCGTGGACAGCTGCGCTTGTCGGCGACCCGCAAACAGTACGTGCCGGTCGTGCAGGAGTTCTTTCGGTCGCTATAA

Protein

MKEFLNNIVNKLKSNDYDNINIVIGNESCDLDSAVCSIVYALYLNWQHNQIKCKVCTKDKRGASSKDDIFIPILNMVRQDFALKTEVMYLFNKLGITEGDLIFRNDYDLCSLLRDSKCNVVLVDHHVLAANDVFLSAFVTEIIDHRPLDKSRWTYKGDTRLIIEIVGSCATLVTRRIKEMCLLLGKEFQFFKDHMPCSQMLYSTIILDTVNFSKEFNKGTPEDEEMIDLLERVLMIENPIDERQRVLDELTKAKSDVSKLTASQLLRKDVKIVEDVLIPSFPMLVEEFLRLDDAVDAVKEVLSQRECLVALLLGMDLTSGMKRDMAVMSPNNENLAEKLDSFLVEWSSPSFELRPRDGAVRGQLRLSATRKQYVPVVQEFFRSL

Description

Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, migration and differentiation, and acts as a negative regulator of NME1. Plays a role in the regulation of neurogenesis. Involved in the regulation of microtubule polymerization.

Catalytic Activity

diphosphate + H2O = H(+) + 2 phosphate

Cofactor

Mn(2+)

Subunit

Homooligomer. Able to homodimerize via its C-terminal domain. Interacts with NME1. Interacts with GSK3; at focal adhesion complexes where paxillin and vinculin are colocalized. Interacts with alpha and beta tubulin.

Similarity

Belongs to the PPase class C family. Prune subfamily.

Keywords

Acetylation   Cell junction   Complete proteome   Cytoplasm   Hydrolase   Manganese   Metal-binding   Nucleus   Phosphoprotein   Reference proteome  

Feature

chain  Exopolyphosphatase PRUNE1

Uniprot

H9IWZ0   A0A2W1B4Z9   A0A2W1BD41   A0A2A4JMY7   I4DNQ3   A0A0N1IN50   + More
A0A2H1WLK3   A0A1E1W890   A0A232F225   A0A067RFC9   A0A034VN65   A0A1Y9HAL3   W8BQY8   A0A1I8NM85   A0A0K8U8M8   A0A0N7ZC27   Q16PL5   A0A1S4FTQ3   B0WDJ6   Q7QAC2   A0A182V4V2   A0A182WQN4   A0A182IIP9   A0A2J7QVK9   A0A1B6I6P8   B4Q197   A0A1B0FCV4   W5JHB6   B3P8X7   A0A182NZ79   A0A182LRY8   A0A1B0ASV6   A0A182XUF5   A0A1A9XYF3   A0A2M4BQX7   O18399   A0A1W4VRY7   A0A182XWS2   B3MYR8   B4I9R1   Q629J6   U5EQB2   A0A182HCD3   A0A182S4W7   D6WGZ0   A0A0L0C1X1   A0A1A9UUW3   A0A1A9ZLR7   A0A182JNP7   A0A0M5J321   A0A1I8M963   A0A088A3H6   A0A2A3E551   V9I9N6   A0A158P1G0   A0A182PYX4   A0A1V4KMK5   A0A026X2L9   A0A1B6GWT5   A0A1D1UYU9   B4JWS0   A0A0M9A9A8   B4NQ14   A0A154NYN6   A0A1A9X047   A0A3B0K5H3   E9IJM2   Q29JM0   A0A151M5B9   A0A151WZQ6   A0A2D4FRL1   A0A151M5C3   A0A1W4X374   A0A1W4X4E6   A0A1Y1KW22   E2C9R2   B4L7V1   T1JHM1   A0A084W795   A0A0F7ZBN7   H9GAR5   Q8BIW1   A0A1S3WM72   A0A195D2L6   A0A1B6KJ61   A0A182SGT7   A0A2Y9L7C0   E2AQE7   F4WYJ6   H0Z2X1   A0A226MKK7   A0A2M4AYE5   A0A182G070   U6CUD4   A0A2M4AYF0   A0A2P4SE01   A0A1B6E685   E0VEZ7  

EC Number

3.6.1.1

Pubmed

19121390    28756777    22651552    26354079    28648823    24845553    + More
25348373    24495485    17510324    12364791    14747013    17210077    17994087    17550304    20920257    23761445    8978047    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    26109357    26109356    25244985    1654526    26483478    18362917    19820115    26108605    25315136    21347285    24508170    30249741    27649274    21282665    15632085    22293439    28004739    20798317    24438588    21881562    10602478    16141072    15489334    21183079    21719571    20360741    20566863   

EMBL

BABH01021143    KZ150416    PZC70958.1    KZ150196    PZC72321.1    NWSH01000996    + More
PCG73179.1    AK403077    BAM19543.1    KQ458725    KPJ05346.1    ODYU01009456    SOQ53908.1    GDQN01007889    JAT83165.1    NNAY01001195    OXU24785.1    KK852669    KDR18853.1    GAKP01015028    JAC43924.1    AXCN02001165    GAMC01005108    JAC01448.1    GDHF01029376    GDHF01002833    JAI22938.1    JAI49481.1    GDRN01074168    JAI63280.1    CH477778    EAT36305.1    DS231898    EDS44605.1    AAAB01008898    EAA09205.5    APCN01004968    NEVH01009907    PNF32606.1    GECU01025105    JAS82601.1    CM000162    EDX01404.2    CCAG010007386    ADMH02001254    ETN63451.1    CH954183    EDV45582.1    AXCM01000922    JXJN01003017    GGFJ01006037    MBW55178.1    AE014298    AY051502    AJ001693    AL009194    AAF45743.1    AAK92926.1    CAA04931.1    CAA15699.1    CH902632    EDV32762.1    CH480825    EDW43942.1    S55520    AAA04887.1    GANO01003346    JAB56525.1    JXUM01127208    KQ567160    KXJ69594.1    KQ971321    EFA00136.2    JRES01000994    KNC26353.1    CP012528    ALC48998.1    KZ288403    PBC26201.1    JR036735    AEY57357.1    ADTU01006530    LSYS01002801    OPJ85696.1    KK107024    QOIP01000004    EZA62256.1    RLU23435.1    GECZ01002890    JAS66879.1    BDGG01000002    GAU94621.1    CH916376    EDV95196.1    KQ435724    KOX78499.1    CH964291    EDW86239.1    KQ434783    KZC04737.1    OUUW01000003    SPP78708.1    GL763868    EFZ19233.1    CH379063    EAL32281.2    AKHW03006582    KYO19713.1    KQ982642    KYQ53247.1    IACJ01079775    LAA50092.1    KYO19714.1    GEZM01074968    JAV64370.1    GL453897    EFN75302.1    CH933814    EDW05526.1    JH431734    ATLV01021177    ATLV01021178    KE525314    KFB46089.1    GBEX01002671    JAI11889.1    AAWZ02034734    AF051908    AK081637    AK142160    BC057546    BC058635    KQ976986    KYN06614.1    GEBQ01028498    JAT11479.1    GL441723    EFN64361.1    GL888450    EGI60728.1    ABQF01039181    MCFN01000693    OXB55833.1    GGFK01012492    MBW45813.1    HAAF01002504    CCP74330.1    GGFK01012493    MBW45814.1    PPHD01059114    POI22337.1    GEDC01003873    JAS33425.1    DS235100    EEB11971.1   

Proteomes

UP000005204    UP000218220    UP000053268    UP000215335    UP000027135    UP000075886    + More
UP000095300    UP000008820    UP000002320    UP000007062    UP000075903    UP000075920    UP000075840    UP000235965    UP000002282    UP000092444    UP000000673    UP000008711    UP000075884    UP000075883    UP000092460    UP000076407    UP000092443    UP000000803    UP000192221    UP000076408    UP000007801    UP000001292    UP000069940    UP000249989    UP000075900    UP000007266    UP000037069    UP000078200    UP000092445    UP000075881    UP000092553    UP000095301    UP000005203    UP000242457    UP000005205    UP000075885    UP000190648    UP000053097    UP000279307    UP000186922    UP000001070    UP000053105    UP000007798    UP000076502    UP000091820    UP000268350    UP000001819    UP000050525    UP000075809    UP000192223    UP000008237    UP000009192    UP000030765    UP000001646    UP000000589    UP000079721    UP000078542    UP000075901    UP000248482    UP000000311    UP000007755    UP000007754    UP000198323    UP000069272    UP000009046   

PRIDE

H9IWZ0    O18399    Q629J6    Q8BIW1   

Pfam

PF01368   DHH        + More
PF02833   DHHA2
PF07714   Pkinase_Tyr
PF00754   F5_F8_type_C

Interpro

IPR038763   DHH_sf        + More
IPR001667   DDH_dom       
IPR004097   DHHA2       
IPR038222   DHHA2_dom_sf       
IPR011009   Kinase-like_dom_sf       
IPR001245   Ser-Thr/Tyr_kinase_cat_dom       
IPR000421   FA58C       
IPR008979   Galactose-bd-like_sf       
IPR000719   Prot_kinase_dom       
IPR008266   Tyr_kinase_AS       

SUPFAM

SSF64182   SSF64182        + More
SSF49785   SSF49785       
SSF56112   SSF56112       

Gene 3D

3.10.310.20     2.60.120.260    

CDD

cd00057  FA58C

ProteinModelPortal

H9IWZ0   A0A2W1B4Z9   A0A2W1BD41   A0A2A4JMY7   I4DNQ3   A0A0N1IN50   + More
A0A2H1WLK3   A0A1E1W890   A0A232F225   A0A067RFC9   A0A034VN65   A0A1Y9HAL3   W8BQY8   A0A1I8NM85   A0A0K8U8M8   A0A0N7ZC27   Q16PL5   A0A1S4FTQ3   B0WDJ6   Q7QAC2   A0A182V4V2   A0A182WQN4   A0A182IIP9   A0A2J7QVK9   A0A1B6I6P8   B4Q197   A0A1B0FCV4   W5JHB6   B3P8X7   A0A182NZ79   A0A182LRY8   A0A1B0ASV6   A0A182XUF5   A0A1A9XYF3   A0A2M4BQX7   O18399   A0A1W4VRY7   A0A182XWS2   B3MYR8   B4I9R1   Q629J6   U5EQB2   A0A182HCD3   A0A182S4W7   D6WGZ0   A0A0L0C1X1   A0A1A9UUW3   A0A1A9ZLR7   A0A182JNP7   A0A0M5J321   A0A1I8M963   A0A088A3H6   A0A2A3E551   V9I9N6   A0A158P1G0   A0A182PYX4   A0A1V4KMK5   A0A026X2L9   A0A1B6GWT5   A0A1D1UYU9   B4JWS0   A0A0M9A9A8   B4NQ14   A0A154NYN6   A0A1A9X047   A0A3B0K5H3   E9IJM2   Q29JM0   A0A151M5B9   A0A151WZQ6   A0A2D4FRL1   A0A151M5C3   A0A1W4X374   A0A1W4X4E6   A0A1Y1KW22   E2C9R2   B4L7V1   T1JHM1   A0A084W795   A0A0F7ZBN7   H9GAR5   Q8BIW1   A0A1S3WM72   A0A195D2L6   A0A1B6KJ61   A0A182SGT7   A0A2Y9L7C0   E2AQE7   F4WYJ6   H0Z2X1   A0A226MKK7   A0A2M4AYE5   A0A182G070   U6CUD4   A0A2M4AYF0   A0A2P4SE01   A0A1B6E685   E0VEZ7  

PDB

2QB8     E-value=2.22795e-14,     Score=192

KEGG

101736746    K01514 exopolyphosphatase [EC:3.6.1.11] | (RefSeq) protein prune homolog

PATHWAY

00230    Purine metabolism - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0005737   GO:0016462   GO:0004309   GO:0006798   GO:0005759   GO:0043951   GO:0019889   GO:0001745   GO:0090297   GO:0048072   GO:0004112   GO:0016021   GO:0004672   GO:0005524   GO:0050767   GO:0031113   GO:0016791   GO:0015631   GO:0005829   GO:0004427   GO:0046872   GO:0005634   GO:0005925   GO:0006355   GO:0003700   GO:0043565   GO:0004559   GO:0006013   GO:0015923   GO:0006260  

Subcellular location

Cytoplasm  
Nucleus  
Cell junction  
Focal adhesion  

Length:

384

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.0415800000000001

Exp number, first 60 AAs:

0.03042

Total prob of N-in:

0.01126

outside

1  -  384

 

 

Pi

274.56163

Theta

202.230249

Tajima's D

1.478369

CLR

0.323607

CSRT

0.785960701964902

Interpretation

Uncertain