Gene

KWMTBOMO06056  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA002846

Annotation

PREDICTED:_ester_hydrolase_C11orf54_homolog_isoform_X1_[Bombyx_mori]

Location

Bomo_Chr10(+):17131646-17139273      View in EpiBrowser

Full name

Cytoplasmic tRNA 2-thiolation protein 1      

Alternative Name

Cytoplasmic tRNA adenylyltransferase 1

Location in the cell

Cytoplasmic   Reliability : 2.036

CDS

ATGACGTCAATAGATTACACTAAAGTAACAATCAAGGAGAAGGAACTATATACTCCTCCGTTGGACGAAGTAGCTTGTGTATTGTCGAATGGTTTAACAACGAACTTCAAGTTTGTGGAAGTGTCAGTGGCCGATAGTCCAGACCTCACTGAACCTCCTTACTATCTCAAATCACCAGGATTGACTGGCGACGCCAAACTGGTCGAGATCGGGGGCCCGCCGTACTTAGTGCCGCAGGTCAAACGCGACAAGATATACGACCTGGCCAAGCTGCTGGAACACTTGAACCGCGACCCCGCCTTCCTCGCCGGCGCTGGAGCCGGACCGTGGCCGTACCTGGGCGTGAACTGCGAGGGTATAGTAAACCTTAGTGTCCGCAACGGTACTGTTGACCAAGGTACCAGAATAGTGTCGGTGCACCCAGTAGGAGCGCCCAAGGGCAGCAGCGGCTACCTCCAGCAACAGCTGCCCAACGACGAGACCAGGACTGCGTTGCTCGGAAACTACCTGCTGACGGAAGGGAAACCCGGAAAGGTAATTAAAGTGGTCGCGAAGAACCGCACGGGCAAGTCCAACTTTATCACGTCCATCCGAGAGACATTGAAGACTCACTACGGAGACAAAGTCGTGGGTCTGGGCGGCGCGTTCGTGCTGCGCGCGGGCCGCGGCTACTTCCACGTGATGCCGGACTTCTCCCGCGCGCCGCTGTGCTCCGACGCCGCCGTGGACTCCTGGCTGCACTACTTCGAGCTGGACGCGCCGCTCACTGTCGTCGGCACGCTCGTCACGGGAGACCTCGGACTGGACCTCCGCGTGCAGCACTTCCACGGGTTCAGCGGCGGCGGCGGGGGCGCGGGGGCGGGCGGCCACTACCACTACGACACCACACCGGAGGACGTGCACTATGAGGGCTACTTCACCGTCGCCTCCTCCCTCATAAGAGTCGACCCTCCCGTCGAGACCCACACCTTCGGACGGGACTAG

Protein

MTSIDYTKVTIKEKELYTPPLDEVACVLSNGLTTNFKFVEVSVADSPDLTEPPYYLKSPGLTGDAKLVEIGGPPYLVPQVKRDKIYDLAKLLEHLNRDPAFLAGAGAGPWPYLGVNCEGIVNLSVRNGTVDQGTRIVSVHPVGAPKGSSGYLQQQLPNDETRTALLGNYLLTEGKPGKVIKVVAKNRTGKSNFITSIRETLKTHYGDKVVGLGGAFVLRAGRGYFHVMPDFSRAPLCSDAAVDSWLHYFELDAPLTVVGTLVTGDLGLDLRVQHFHGFSGGGGGAGAGGHYHYDTTPEDVHYEGYFTVASSLIRVDPPVETHTFGRD

Description

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.

Similarity

Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.

Uniprot

H9J011   A0A2W1BLV6   A0A2H1V853   A0A2A4J2B8   A0A2A4J2U6   A0A212FEE3   + More
A0A0N1I8F1   A0A194Q3V1   D6X2L2   A0A2P8Y732   A0A0L7R706   A0A067R896   Q16EA5   A0A088A055   Q17B27   A0A1S4G452   A0A2J7RE41   A0A182GD19   A0A1Y1K938   A0A2A3E5R7   J3JZE1   A0A1W4XLR3   N6UBV7   U4UGN1   A0A154P5G1   A0A1Q3FQ17   B0WLB0   K7ITL9   V5GX61   A0A084WLJ3   V4AUJ8   U5EUR4   A0A182N7N2   A0A195BZX3   A0A182IKE7   E0VTT7   A0A151XBQ3   A0A023EMS0   A0A0C9Q6Z5   A0A182QH76   A0A0A9XZV0   E2A097   A0A0K8TRB6   B4PCR7   A0A164WN88   A0A182PHV0   A0A131XXW9   A0A336LW91   A0A0J9RLX0   B3NJF5   A0A0P8Y9F4   Q9W0J9   A0A182TAJ0   A0A0P5T6V4   E9FYW1   A0A1W4W6F7   A0A182WIA3   A0A232ENF9   B4HVN8   A0A3R7NU13   A0A0A9XUG4   B4QLX7   A0A182R2I3   A0A195EGD2   A0A182YNL0   A0A1B0ESX2   F4WG09   A0A182TFX9   A0A182X1A4   T1DFP0   Q7QI91   A0A0P6CPY2   R4WD98   B4MXC9   A0A182KLG2   A0A182I4V0   A0A151JWK2   A0A0P5DI34   A0A131YK09   A0A3B0JWG8   A0A0P6I9W1   A0A0L0CES6   L7M6I0   A0A1E1XRP5   A0A224YDN9   E2A096   A0A1E1X8H5   A0A2M4AQE5   W5JIY8   A0A158NTF7   A0A1I8P2H9   A0A1L8DD28   E9IBH5   A0A0J7KT14   A0A131XHN0   A0A2M4BTS8   A0A1Y1KGT9   A0A3L8D7T7   A0A1L8DD08   A0A2M4AQD5  

EC Number

2.7.7.-

Pubmed

19121390    28756777    22118469    26354079    18362917    19820115    + More
29403074    24845553    17510324    26483478    28004739    22516182    23537049    20075255    24438588    23254933    20566863    24945155    25401762    20798317    26369729    17994087    17550304    29652888    22936249    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    26109357    26109356    21292972    28648823    26823975    25244985    21719571    12364791    14747013    17210077    23691247    20966253    26830274    26108605    25576852    29209593    28797301    28503490    20920257    23761445    21347285    21282665    28049606    30249741   

EMBL

BABH01018836    BABH01018837    KZ150039    PZC74594.1    ODYU01001174    SOQ37028.1    + More
NWSH01003638    PCG66009.1    PCG66008.1    AGBW02008947    OWR52104.1    KQ460784    KPJ12215.1    KQ459472    KPJ00218.1    KQ971372    EFA09460.2    PYGN01000858    PSN39974.1    KQ414643    KOC66667.1    KK852817    KDR15774.1    CH478915    EAT32561.1    CH477327    EAT43443.1    NEVH01005278    PNF39103.1    JXUM01009508    JXUM01009509    JXUM01009510    JXUM01009511    KQ560285    KXJ83280.1    GEZM01088781    GEZM01088780    GEZM01088779    GEZM01088776    GEZM01088775    GEZM01088774    GEZM01088773    GEZM01088769    GEZM01088764    JAV57943.1    KZ288379    PBC26596.1    BT128622    AEE63579.1    APGK01029461    KB740735    ENN79140.1    KB632136    ERL89050.1    KQ434809    KZC06360.1    GFDL01005388    JAV29657.1    DS231983    EDS30394.1    GALX01003568    JAB64898.1    ATLV01024247    KE525350    KFB51087.1    KB201305    ESO97446.1    GANO01001352    JAB58519.1    KQ978501    KYM93458.1    DS235773    EEB16793.1    KQ982320    KYQ57784.1    GAPW01002946    JAC10652.1    GBYB01009898    JAG79665.1    AXCN02000581    GBHO01019221    JAG24383.1    GL435556    EFN73157.1    GDAI01000930    JAI16673.1    CM000159    EDW92790.1    LRGB01001151    KZS13421.1    GEFM01005365    GEGO01001406    JAP70431.1    JAR93998.1    UFQS01000244    UFQT01000244    SSX01945.1    SSX22322.1    CM002912    KMY96762.1    CH954178    EDV50117.1    CH902618    KPU78080.1    AE014296    AY058311    AAF47447.1    AAL13540.1    GDIP01134562    JAL69152.1    GL732527    EFX87705.1    NNAY01003177    OXU19862.1    CH480817    EDW50003.1    QCYY01002901    ROT66710.1    GBHO01019222    GBRD01003635    GBRD01003634    GDHC01016910    JAG24382.1    JAG62186.1    JAQ01719.1    CM000363    EDX08763.1    KQ978957    KYN26962.1    AJWK01005667    GL888128    EGI66776.1    GAMD01003086    JAA98504.1    AAAB01008807    EAA04694.3    GDIQ01088545    JAN06192.1    AK417349    BAN20564.1    CH963876    EDW76962.1    APCN01002356    KQ981639    KYN38685.1    GDIP01157154    JAJ66248.1    GEDV01009672    JAP78885.1    OUUW01000009    SPP84782.1    GDIQ01007541    JAN87196.1    JRES01000487    KNC30888.1    GACK01005562    JAA59472.1    GFAA01001444    JAU01991.1    GFPF01004672    MAA15818.1    EFN73156.1    GFAC01003623    JAT95565.1    GGFK01009686    MBW43007.1    ADMH02001359    ETN62850.1    ADTU01025886    GFDF01009732    JAV04352.1    GL762111    EFZ22163.1    LBMM01003570    KMQ93374.1    GEFH01001958    JAP66623.1    GGFJ01007334    MBW56475.1    GEZM01088783    GEZM01088770    GEZM01088767    GEZM01088765    GEZM01088762    JAV57957.1    QOIP01000012    RLU16321.1    GFDF01009733    JAV04351.1    GGFK01009685    MBW43006.1   

Proteomes

UP000005204    UP000218220    UP000007151    UP000053240    UP000053268    UP000007266    + More
UP000245037    UP000053825    UP000027135    UP000008820    UP000005203    UP000235965    UP000069940    UP000249989    UP000242457    UP000192223    UP000019118    UP000030742    UP000076502    UP000002320    UP000002358    UP000030765    UP000030746    UP000075884    UP000078542    UP000075880    UP000009046    UP000075809    UP000075886    UP000000311    UP000002282    UP000076858    UP000075885    UP000008711    UP000007801    UP000000803    UP000075901    UP000000305    UP000192221    UP000075920    UP000215335    UP000001292    UP000283509    UP000000304    UP000075900    UP000078492    UP000076408    UP000092461    UP000007755    UP000075902    UP000076407    UP000007062    UP000007798    UP000075882    UP000075840    UP000078541    UP000268350    UP000037069    UP000000673    UP000005205    UP000095300    UP000036403    UP000279307   

PRIDE

H9J011    A0A088A055    Q9W0J9   

Pfam

PF08925   DUF1907        + More
PF16503   zn-ribbon_14
PF01171   ATP_bind_3

Interpro

IPR015021   DUF1907        + More
IPR014729   Rossmann-like_a/b/a_fold       
IPR032442   CTU1_C       
IPR000541   Ncs6/Tuc1/Ctu1       
IPR011063   TilS/TtcA_N       
IPR020554   UPF0021_CS       

Gene 3D

3.40.50.620    

ProteinModelPortal

H9J011   A0A2W1BLV6   A0A2H1V853   A0A2A4J2B8   A0A2A4J2U6   A0A212FEE3   + More
A0A0N1I8F1   A0A194Q3V1   D6X2L2   A0A2P8Y732   A0A0L7R706   A0A067R896   Q16EA5   A0A088A055   Q17B27   A0A1S4G452   A0A2J7RE41   A0A182GD19   A0A1Y1K938   A0A2A3E5R7   J3JZE1   A0A1W4XLR3   N6UBV7   U4UGN1   A0A154P5G1   A0A1Q3FQ17   B0WLB0   K7ITL9   V5GX61   A0A084WLJ3   V4AUJ8   U5EUR4   A0A182N7N2   A0A195BZX3   A0A182IKE7   E0VTT7   A0A151XBQ3   A0A023EMS0   A0A0C9Q6Z5   A0A182QH76   A0A0A9XZV0   E2A097   A0A0K8TRB6   B4PCR7   A0A164WN88   A0A182PHV0   A0A131XXW9   A0A336LW91   A0A0J9RLX0   B3NJF5   A0A0P8Y9F4   Q9W0J9   A0A182TAJ0   A0A0P5T6V4   E9FYW1   A0A1W4W6F7   A0A182WIA3   A0A232ENF9   B4HVN8   A0A3R7NU13   A0A0A9XUG4   B4QLX7   A0A182R2I3   A0A195EGD2   A0A182YNL0   A0A1B0ESX2   F4WG09   A0A182TFX9   A0A182X1A4   T1DFP0   Q7QI91   A0A0P6CPY2   R4WD98   B4MXC9   A0A182KLG2   A0A182I4V0   A0A151JWK2   A0A0P5DI34   A0A131YK09   A0A3B0JWG8   A0A0P6I9W1   A0A0L0CES6   L7M6I0   A0A1E1XRP5   A0A224YDN9   E2A096   A0A1E1X8H5   A0A2M4AQE5   W5JIY8   A0A158NTF7   A0A1I8P2H9   A0A1L8DD28   E9IBH5   A0A0J7KT14   A0A131XHN0   A0A2M4BTS8   A0A1Y1KGT9   A0A3L8D7T7   A0A1L8DD08   A0A2M4AQD5  

PDB

1XCR     E-value=6.59249e-55,     Score=540

KEGG

101745847    no KO assigned | (RefSeq) ester hydrolase C11orf54 homolog

GO

GO:0005634   GO:0016787   GO:0016021   GO:0008270   GO:0016788   GO:0034227   GO:0000049   GO:0032447   GO:0002098   GO:0016779   GO:0005737   GO:0019901   GO:0008234   GO:0008026   GO:0016818   GO:0004003   GO:0003677   GO:0000723   GO:0006260  

PANTHER

PTHR13204   PTHR11807:SF12  

Subcellular location

Cytoplasm  

Length:

327

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.06345

Exp number, first 60 AAs:

0.00042

Total prob of N-in:

0.08351

outside

1  -  327

 

 

Pi

3.710837

Theta

6.85506

Tajima's D

0.187749

CLR

0.6661

CSRT

0.44282785860707

Interpretation

Uncertain