Gene

KWMTBOMO06030

Pre Gene Modal

BGIBMGA002868

Annotation

PREDICTED:_D-glucuronyl_C5-epimerase_isoform_X1_[Bombyx_mori]

Location

Bomo_Chr10(-):16783266-16789011      View in EpiBrowser

Full name

D-glucuronyl C5-epimerase       + More
D-glucuronyl C5-epimerase B      

Alternative Name

Heparan sulfate C5-epimerase
Heparin sulfate C5-epimerase
Heparin/heparan sulfate:glucuronic acid C5-epimerase
Heparosan-N-sulfate-glucuronate 5-epimerase

Location in the cell

Extracellular   Reliability : 0.875

CDS

ATGCTGGTGCGTATGAACATGAAGACAGTGCTGGGCGCTCTCTGCGTCGCAGTACTGCTCTTCACTCTATTCTGGGGACACTGTGGTGATTTCTCTAGAAGACCTTTAGGTTCAGTTCTGTCTGAGCATGGTCACGGTGCAGATGACATTGAATGCATAATTAACGGAGAGTATTCAGTGTCATGTCGGCGGGATCGCGAGAGAGACGACGTATATGTGCCTTTCTCACTTGTACATAAGTATTTTGAGATATACGGTAAGATAACTACAACGGACGGCGTTGAAAAATTCGAATGGTCGCATAGTTACGGCAAGATATACCATCCTAAGAAGAAATACGACCCGCGCGGCACATTCACCACGTTTGAGAACTACAACGTTGAAGTACGTGACAGAGTGAAATGCATCAGCGGGATCGAAGGAGTGCCGATCTCTACTCAATGGGACCCCAAGGGTTTCTTTTACCCCACCCAAATAGCCCAGTTCGGTCTGGCCCACTACAGTAAGTTCCTGACCGAAGCCGAACCGAGACGGAGGATCATCGATGATGGCGAGAAACATATAGAGAATTGGATCGTCAGCAAAGATGCCTTTATGGTCAGGGAGTATGACTCAGAATCACAAACAAATATTCTAAGGTTTTCTACGTCAGACCACGTGTCGAGTCAAGTGTGGCTGAAAGTGAACATTAGTCAGGATTTCGTGCTGAGTGCCGATGTGATGATGAAGCCCAACTCCTCGATGACAGTTGTACTGCAGAATAAGGAAAAGAAGGAAACCGTTTATCTCCACTATGTTGCGAGTACACAGCTTATTTATGCTCAAGATGATCACATTTACTACGGCATCGGCGTGGAACAGAAATGGAGGAAGATAACGAGAGATCTCATAATCGACATGCAGAAGGGTTGGATCTTACAGGATAGACCCAAACGGAGGTCTCCTAGGAATAAATTTAAGATATCAAGTGTAATACTGAGCGGCACGGGCGGGCTGGACAACGTGACGGTGTCGTCCAGCGAGCACATGGCGCAGTTCTACGCGGCGGCGAGCTGGCTGGTGCGGGCGCAGCGCGGGCGCGGCGGCTGGCCCGTGCCGGCGCGCCGCCGGGTGGCCGCCGGGGTCGCCGACCTCGCGCCCGGATGGTACTCCGCGATGAGCCAGGGCCACGCCATCTCGGTGCTGGCGCGCGCGTACTCCCGCAGCGGTGACGTCACGTACCTGCAGGCCGCCCGCCGCGCGCTCTACGTGCTGGACGTGCCGAGCCACGCCGGTGGGGTCAAGGCCCTGTGGATGGACAAGTTTGTTTGGTACGAAGAATACCCAACGAAGCCTCCGCTTTTCGTTCTAAACGGCTTCATCTACACGCTCCTCGGTCTCTACGACCTGCACGTCATTGAAGGTGAGAACTCCATGTCTCTAGCCAAGAAGATGTTTGACGACGGAATGACGTCACTCAAAACTCTATTACCACTGTTCGACACCGGCAGCGGAAGCTTCTACGACCTGAGGCATTTCACGTTGGGAGTCAGCCCCAATATAGCCAGGTGGGACTACCACGCCACCCACGTTAACCAGCTTTACCTTTTAGCTGGATTGGACGATGATCCTATACTACTGAACACGGCCAAAAGATGGGAGGGATACATGCAAGGTAAAAGAGCAGCGCATAACTGA

Protein

MLVRMNMKTVLGALCVAVLLFTLFWGHCGDFSRRPLGSVLSEHGHGADDIECIINGEYSVSCRRDRERDDVYVPFSLVHKYFEIYGKITTTDGVEKFEWSHSYGKIYHPKKKYDPRGTFTTFENYNVEVRDRVKCISGIEGVPISTQWDPKGFFYPTQIAQFGLAHYSKFLTEAEPRRRIIDDGEKHIENWIVSKDAFMVREYDSESQTNILRFSTSDHVSSQVWLKVNISQDFVLSADVMMKPNSSMTVVLQNKEKKETVYLHYVASTQLIYAQDDHIYYGIGVEQKWRKITRDLIIDMQKGWILQDRPKRRSPRNKFKISSVILSGTGGLDNVTVSSSEHMAQFYAAASWLVRAQRGRGGWPVPARRRVAAGVADLAPGWYSAMSQGHAISVLARAYSRSGDVTYLQAARRALYVLDVPSHAGGVKALWMDKFVWYEEYPTKPPLFVLNGFIYTLLGLYDLHVIEGENSMSLAKKMFDDGMTSLKTLLPLFDTGSGSFYDLRHFTLGVSPNIARWDYHATHVNQLYLLAGLDDDPILLNTAKRWEGYMQGKRAAHN

Description

Converts D-glucuronic acid residues adjacent to N-sulfate sugar residues to L-iduronic acid residues, both in maturing heparan sulfate (HS) and heparin chains. This is important for further modifications that determine the specificity of interactions between these glycosaminoglycans and proteins.
Converts D-glucuronic acid residues adjacent to N-sulfate sugar residues to L-iduronic acid residues, both in maturing heparan sulfate (HS) and heparin chains (PubMed:16156897, PubMed:25568314). Plays a role in dorso-ventral axis specification during early development, together with glcea, where it may potentiate signaling via the BMP pathway (PubMed:16156897).

Catalytic Activity

[heparosan-N-sulfate](n) = [heparan-N-sulfate](n)

Subunit

Homodimer (By similarity). Interacts with HS2ST1 (PubMed:11687650).
Homodimer.

Similarity

Belongs to the D-glucuronyl C5-epimerase family.

Keywords

Complete proteome   Golgi apparatus   Isomerase   Membrane   Reference proteome   Signal-anchor   Transmembrane   Transmembrane helix   3D-structure  

Feature

chain  D-glucuronyl C5-epimerase

Uniprot

H9J033   A0A2H1VPK7   A0A3S2NMA6   A0A194Q5B3   A0A212FNT6   A0A1Y1K8J4   + More
A0A026WTR0   E9IBW9   E2AIU8   A0A151X8Y2   A0A195FCD7   F4X3F8   A0A195CJ32   A0A195DG75   E2BA51   A0A195BNB8   A0A158NKQ0   A0A0M8ZSC3   D6WZ61   A0A2J7R763   A0A2A3E6U4   A0A1B6CJP8   A0A0T6BCN4   A0A067RHE1   A0A154PPK9   A0A2J7R769   A0A1B6JB23   A0A088AEE1   A0A1B6LT54   A0A1W4WIR8   A0A1S4F904   A0A182H981   A0A182FE78   K7IRZ7   W5J9K2   Q17BP7   A0A182HWN9   A0A182VF35   A0A182XMT2   A0A2M4AL95   A0A310S5B4   B0X473   A0A182U054   A0A182WIE5   A0A182Q6E6   Q7PPB9   A0A182ISF5   A0A182R2E3   A0A182Y1D7   A0A182M3A3   A0A182K097   A0A182NH00   A0A182PHR4   A0A1L8E207   A0A1J1IU63   A0A232F4W1   A0A1Q3F7Y3   A0A069DVG4   N6T6D4   U4UG77   A0A023F1C8   T1HL57   A0A0K8TMG3   A0A2K8JMB6   A0A2P8Y291   A0A224XM29   Q293A0   B4GC68   A0A0A9ZDX8   A0A087UKA2   A0A0K8SUY8   E9H5V8   A0A182LIY5   A0A0P6E1Q6   A0A1W4V1I1   S4R9B5   T1JNE4   U3IEB6   A0A3Q0SKB5   A0A3B5KTY5   A0A3Q1AIE2   Q9EPS3   B4KNJ2   G1M6G4   A0A146NUP7   I3K685   B4LK01   A0A2R8RZG5   F1QR43   A0A3P8RQ55   A0A3B4FZ27   A0A3Q2VN81   A0A3P8PXZ2   A0A3Q1GST1   A0A1S3EUJ9   H3AZA6   B3NB42   A0A1U7QBR3   B4J6I7  

EC Number

5.1.3.17

Pubmed

19121390    26354079    22118469    28004739    24508170    30249741    + More
21282665    20798317    21719571    21347285    18362917    19820115    24845553    26483478    20075255    20920257    23761445    17510324    12364791    25244985    28648823    26334808    23537049    25474469    26369729    29403074    15632085    17994087    23185243    25401762    26823975    21292972    20966253    23749191    21551351    11274177    11279150    15489334    11687650    12788935    16532012    20208005    20010809    25186727    23594743    16156897    17195182    25568314    9215903   

EMBL

BABH01018869    BABH01018870    ODYU01003427    SOQ42174.1    RSAL01000262    RVE43320.1    + More
KQ459472    KPJ00195.1    AGBW02004392    OWR55408.1    GEZM01089087    JAV57723.1    KK107105    QOIP01000011    EZA59450.1    RLU16852.1    GL762137    EFZ21994.1    GL439905    EFN66627.1    KQ982409    KYQ56822.1    KQ981693    KYN37867.1    GL888613    EGI58986.1    KQ977649    KYN00735.1    KQ980886    KYN11842.1    GL446664    EFN87460.1    KQ976439    KYM86713.1    ADTU01018958    KQ435908    KOX68966.1    KQ971372    EFA10381.1    NEVH01006738    PNF36685.1    KZ288355    PBC27224.1    GEDC01023650    JAS13648.1    LJIG01001852    KRT85088.1    KK852470    KDR23187.1    KQ435012    KZC13826.1    PNF36683.1    GECU01011361    JAS96345.1    GEBQ01013111    JAT26866.1    JXUM01120197    KQ566369    KXJ70179.1    ADMH02001764    ETN61147.1    CH477319    EAT43666.1    APCN01001514    GGFK01008239    MBW41560.1    KQ770367    OAD52654.1    DS232330    EDS40150.1    AXCN02000347    AAAB01008960    EAA10732.5    AXCM01004288    GFDF01001337    JAV12747.1    CVRI01000059    CRL03803.1    NNAY01001009    OXU25503.1    GFDL01011447    JAV23598.1    GBGD01001024    JAC87865.1    APGK01042240    KB741002    ENN75779.1    KB632309    ERL92037.1    GBBI01003432    JAC15280.1    ACPB03016147    GDAI01002255    JAI15348.1    KY031063    ATU82814.1    PYGN01001024    PSN38350.1    GFTR01006774    JAW09652.1    CM000071    EAL24711.1    KRT01489.1    CH479181    EDW31386.1    GBHO01000117    GDHC01007473    JAG43487.1    JAQ11156.1    KK120217    KFM77791.1    GBRD01009116    JAG56705.1    GL732595    EFX72799.1    GDIP01142598    GDIQ01069063    LRGB01002894    JAL61116.1    JAN25674.1    KZS05585.1    JH432008    ADON01093822    AF330049    AF325532    CH466522    BC094587    AAG42004.1    CH933808    EDW08951.1    ACTA01123872    GCES01150849    JAQ35473.1    AERX01042747    CH940648    EDW61655.2    BX914196    BC163091    BC163093    AY388517    AAI63091.1    AAQ90467.1    AFYH01080147    AFYH01080148    AFYH01080149    AFYH01080150    AFYH01080151    AFYH01080152    AFYH01080153    CH954177    EDV59807.1    CH916367    EDW01988.1   

Proteomes

UP000005204    UP000283053    UP000053268    UP000007151    UP000053097    UP000279307    + More
UP000000311    UP000075809    UP000078541    UP000007755    UP000078542    UP000078492    UP000008237    UP000078540    UP000005205    UP000053105    UP000007266    UP000235965    UP000242457    UP000027135    UP000076502    UP000005203    UP000192223    UP000069940    UP000249989    UP000069272    UP000002358    UP000000673    UP000008820    UP000075840    UP000075903    UP000076407    UP000002320    UP000075902    UP000075920    UP000075886    UP000007062    UP000075880    UP000075900    UP000076408    UP000075883    UP000075881    UP000075884    UP000075885    UP000183832    UP000215335    UP000019118    UP000030742    UP000015103    UP000245037    UP000001819    UP000008744    UP000054359    UP000000305    UP000075882    UP000076858    UP000192221    UP000245300    UP000016666    UP000261340    UP000005226    UP000257160    UP000000589    UP000009192    UP000008912    UP000005207    UP000008792    UP000000437    UP000265080    UP000261460    UP000264840    UP000265100    UP000257200    UP000081671    UP000008672    UP000008711    UP000189706    UP000001070   

PRIDE

H9J033    Q9EPS3   

Pfam

PF06662   C5-epim_C

Interpro

IPR039721   C5-epimerase        + More
IPR010598   C5-epim_C       
IPR008928   6-hairpin_glycosidase_sf       

SUPFAM

SSF48208   SSF48208       

ProteinModelPortal

H9J033   A0A2H1VPK7   A0A3S2NMA6   A0A194Q5B3   A0A212FNT6   A0A1Y1K8J4   + More
A0A026WTR0   E9IBW9   E2AIU8   A0A151X8Y2   A0A195FCD7   F4X3F8   A0A195CJ32   A0A195DG75   E2BA51   A0A195BNB8   A0A158NKQ0   A0A0M8ZSC3   D6WZ61   A0A2J7R763   A0A2A3E6U4   A0A1B6CJP8   A0A0T6BCN4   A0A067RHE1   A0A154PPK9   A0A2J7R769   A0A1B6JB23   A0A088AEE1   A0A1B6LT54   A0A1W4WIR8   A0A1S4F904   A0A182H981   A0A182FE78   K7IRZ7   W5J9K2   Q17BP7   A0A182HWN9   A0A182VF35   A0A182XMT2   A0A2M4AL95   A0A310S5B4   B0X473   A0A182U054   A0A182WIE5   A0A182Q6E6   Q7PPB9   A0A182ISF5   A0A182R2E3   A0A182Y1D7   A0A182M3A3   A0A182K097   A0A182NH00   A0A182PHR4   A0A1L8E207   A0A1J1IU63   A0A232F4W1   A0A1Q3F7Y3   A0A069DVG4   N6T6D4   U4UG77   A0A023F1C8   T1HL57   A0A0K8TMG3   A0A2K8JMB6   A0A2P8Y291   A0A224XM29   Q293A0   B4GC68   A0A0A9ZDX8   A0A087UKA2   A0A0K8SUY8   E9H5V8   A0A182LIY5   A0A0P6E1Q6   A0A1W4V1I1   S4R9B5   T1JNE4   U3IEB6   A0A3Q0SKB5   A0A3B5KTY5   A0A3Q1AIE2   Q9EPS3   B4KNJ2   G1M6G4   A0A146NUP7   I3K685   B4LK01   A0A2R8RZG5   F1QR43   A0A3P8RQ55   A0A3B4FZ27   A0A3Q2VN81   A0A3P8PXZ2   A0A3Q1GST1   A0A1S3EUJ9   H3AZA6   B3NB42   A0A1U7QBR3   B4J6I7  

PDB

4PXQ     E-value=4.64269e-145,     Score=1320

KEGG

101736603    K01793 heparosan-N-sulfate-glucuronate 5-epimerase [EC:5.1.3.17] | (RefSeq) D-glucuronyl C5-epimeras

PATHWAY

00534    Glycosaminoglycan biosynthesis - heparan sulfate / heparin - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0047464   GO:0015012   GO:0016021   GO:0005794   GO:0030210   GO:0042803   GO:0050379   GO:0000139   GO:0016857   GO:0006024   GO:0008026   GO:0016818   GO:0004003   GO:0003677   GO:0000723   GO:0005634   GO:0006260  

PANTHER

PTHR13174  

Subcellular location

Golgi apparatus membrane  

Length:

558

Number of predicted TMHs:

1

Exp number of AAs in TMHs:

19.9349899999999

Exp number, first 60 AAs:

19.62965

Total prob of N-in:

0.98370

POSSIBLE N-term signal

sequence

inside

1  -  8

TMhelix

9  -  26

outside

27  -  558

 

 

Pi

13.348091

Theta

17.202053

Tajima's D

-0.519158

CLR

1.005094

CSRT

0.238838058097095

Interpretation

Uncertain