Gene

KWMTBOMO06010

Annotation

PREDICTED:_alpha-ketoglutarate-dependent_dioxygenase_alkB_homolog_2-like_[Papilio_polytes]

Location

Bomo_Chr10(+):16061302-16063222      View in EpiBrowser

Full name

DNA oxidative demethylase ALKBH2      

Alternative Name

Alkylated DNA repair protein alkB homolog 2
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2
Oxy DC1

Location in the cell

Cytoplasmic   Reliability : 1.068 Mitochondrial   Reliability : 1.593

CDS

ATGGTATACAAATTGGTTTTTGGCAAAACGTATCCTTTGCCCAGACAACAAGTGGCATACGGAGATGATGGCATAACATACACATACTCAGGGGTTACAGTACCTGCCCTACCGTGGCCTGCACCTGTTCTCGCGTTAAGGGATTTTCTTTACGGAATAACGAATATCAGATATGATTTCGTGTTGATAAATAAATACAGAAACGGTATAGATCATATGGGCGAACATAGGGACAATGAACCAGAATTGGACCCAAGCTACCCCATCGCATCTATTTCTTTAGGACAGGAACGACCGTTTGTATTGAAACATAGAGATGCAAGAAAATCTGGAAAAGAGAAAAGGGCAATACCTACAGGTTTGTAG

Protein

MVYKLVFGKTYPLPRQQVAYGDDGITYTYSGVTVPALPWPAPVLALRDFLYGITNIRYDFVLINKYRNGIDHMGEHRDNEPELDPSYPIASISLGQERPFVLKHRDARKSGKEKRAIPTGL

Description

Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron.

Catalytic Activity

2-oxoglutarate + a methylated nucleobase within DNA + O2 = a nucleobase within DNA + CO2 + formaldehyde + succinate

Cofactor

Fe(2+)

Similarity

Belongs to the alkB family.

Keywords

3D-structure   Alternative splicing   Complete proteome   Dioxygenase   DNA damage   DNA repair   Iron   Magnesium   Metal-binding   Nucleus   Oxidoreductase   Polymorphism   Reference proteome  

Feature

chain  DNA oxidative demethylase ALKBH2
splice variant  In isoform 2.
sequence variant  In dbSNP:rs33962311.

Uniprot

A0A2A4JIB4   A0A2H1WQ15   A0A0N1PGK9   A0A194Q3R6   A0A1Y1MM59   A0A0T6B3S1   + More
A0A1B6CYH9   A0A067RC09   D6X1E3   A0A2J7PF86   A0A2J7PF91   A0A0S7MGW3   A0A1A7YV10   A0A2I4BPZ0   M4AUZ1   A0A3P9H4P9   A0A3B5QHA3   A0A3B5Q4E6   A0A1A8NXQ1   H3DEL7   A0A3P9MCG8   A0A1A8PX10   A0A3Q3BCE2   A0A1A8AY50   A0A1A8IXR2   A0A3B3V2J7   A0A1A8G2W4   A0A3P9MCJ2   A0A2D0PP36   A0A1A8UZE3   A0A1A8C4P2   G3P2H6   A0A2D0PRQ7   A0A3B4YX32   A0A2H8TFP4   A0A2H8TL25   A0A3B4UDY2   A0A3B5KLA0   A0A3B3HKF1   A0A3B5K4V9   A0A3Q3MES8   G3P2H0   A0A3Q3JUL3   A0A3Q2PLH1   A0A2S2PBH9   H2LS59   A0A087Y0C3   I3K701   I3K702   A0A3Q1G2I5   A0A147A9H4   A0A2U9BSQ7   A0A3Q2FHA8   A0A3P9CEN5   A0A3Q1HWQ0   A0A3B3B8B5   A0A1B6ISZ2   A0A3P9QGC7   A0A3P8R9T4   A0A3B4GG59   A0A3N0Z0K3   A0A3B3XTA9   A0A3B3XSY1   A0A2U9BS92   A0A3Q0SIH0   C4WWX2   A0A2R5LHV2   A0A3Q3XIP8   A0A3P8U6V0   A0A060WH90   A0A3B1KAJ0   E9QG26   A0A3Q4H1K2   A0A3Q2UZE1   A3QK41   A0A2P2I833   A0A3Q1BN59   A0A3P8Y769   A0A0P4WCI8   G3R2E5   A0A2R9AWD5   H2Q6U0   Q6NS38   G3RZ41   A0A3B4CST3   A0A2G8JT43   A0A2K5HSH0   Q4RSX1   A0A2K6BD08   A0A2K6MNL8   A0A2K6PVN6   A0A2K5KWI2   A0A2K5XJD9   A0A096MYW1   G7PI61   F7DWK4   A0A2K5DI25   A0A2J8XLJ4   A0A3P8VI60   A0A3Q2YVB0  

EC Number

1.14.11.33

Pubmed

26354079    28004739    24845553    18362917    19820115    23542700    + More
17554307    15496914    21551351    25186727    29451363    24755649    25329095    23594743    25069045    22398555    22722832    16136131    17979886    16541075    15489334    12486230    12594517    16174769    18519673    21269460    18432238    20223766    29023486    25362486    22002653    17431167    25319552    24487278   

EMBL

NWSH01001468    PCG71152.1    ODYU01010151    SOQ55066.1    KQ461186    KPJ07389.1    + More
KQ459472    KPJ00177.1    GEZM01029000    JAV86168.1    LJIG01015997    KRT81924.1    GEDC01018769    GEDC01009461    JAS18529.1    JAS27837.1    KK852556    KDR21426.1    KQ971372    EFA10619.1    NEVH01026085    PNF14988.1    PNF14987.1    GBYX01028228    JAP00977.1    HADX01011548    SBP33780.1    HAEG01005309    SBR73806.1    HAEH01009002    SBR86010.1    HADY01020926    SBP59411.1    HAED01015682    SBR02127.1    HAEB01019030    SBQ65557.1    HAEJ01012509    SBS52966.1    HADZ01010931    SBP74872.1    GFXV01001076    MBW12881.1    GFXV01002944    MBW14749.1    GGMR01013657    MBY26276.1    AYCK01007732    AERX01026848    GCES01011162    JAR75161.1    CP026251    AWP07081.1    GECU01017716    JAS89990.1    RJVU01018862    ROL51428.1    AWP07078.1    AWP07079.1    AWP07080.1    ABLF02039828    AK342327    BAH72392.1    GGLE01004984    MBY09110.1    FR904540    CDQ66366.1    CR848794    CR931763    IACF01004580    LAB70169.1    GDRN01063675    GDRN01063673    JAI64942.1    CABD030086578    CABD030086579    AJFE02116885    AC193909    GABC01002660    GABF01008002    NBAG03000240    JAA08678.1    JAA14143.1    PNI64854.1    PNI64857.1    AY754389    AB277859    AC011596    BC070489    MRZV01001305    PIK38879.1    CAAE01014999    CAG08511.1    AHZZ02002947    AQIA01012618    CM001286    EHH66688.1    JSUE03007717    JU322687    JV044372    CM001263    AFE66443.1    AFI34443.1    EHH21155.1    NDHI03003364    PNJ82904.1    PNJ82907.1   

Proteomes

UP000218220    UP000053240    UP000053268    UP000027135    UP000007266    UP000235965    + More
UP000192220    UP000002852    UP000265200    UP000007303    UP000265180    UP000264800    UP000261500    UP000221080    UP000007635    UP000261360    UP000261420    UP000005226    UP000001038    UP000261640    UP000261600    UP000265000    UP000028760    UP000005207    UP000257200    UP000246464    UP000265020    UP000265160    UP000265040    UP000261560    UP000242638    UP000265100    UP000261460    UP000261480    UP000261340    UP000007819    UP000261620    UP000265080    UP000193380    UP000018467    UP000000437    UP000261580    UP000264840    UP000257160    UP000265140    UP000001519    UP000240080    UP000002277    UP000005640    UP000261440    UP000230750    UP000233080    UP000233120    UP000233180    UP000233200    UP000233060    UP000233140    UP000028761    UP000009130    UP000233100    UP000006718    UP000233020    UP000265120    UP000264820   

PRIDE

Q6NS38   

Pfam

PF13532   2OG-FeII_Oxy_2

Interpro

IPR027450   AlkB-like        + More
IPR005123   Oxoglu/Fe-dep_dioxygenase       
IPR037151   AlkB-like_sf       

Gene 3D

2.60.120.590    

ProteinModelPortal

A0A2A4JIB4   A0A2H1WQ15   A0A0N1PGK9   A0A194Q3R6   A0A1Y1MM59   A0A0T6B3S1   + More
A0A1B6CYH9   A0A067RC09   D6X1E3   A0A2J7PF86   A0A2J7PF91   A0A0S7MGW3   A0A1A7YV10   A0A2I4BPZ0   M4AUZ1   A0A3P9H4P9   A0A3B5QHA3   A0A3B5Q4E6   A0A1A8NXQ1   H3DEL7   A0A3P9MCG8   A0A1A8PX10   A0A3Q3BCE2   A0A1A8AY50   A0A1A8IXR2   A0A3B3V2J7   A0A1A8G2W4   A0A3P9MCJ2   A0A2D0PP36   A0A1A8UZE3   A0A1A8C4P2   G3P2H6   A0A2D0PRQ7   A0A3B4YX32   A0A2H8TFP4   A0A2H8TL25   A0A3B4UDY2   A0A3B5KLA0   A0A3B3HKF1   A0A3B5K4V9   A0A3Q3MES8   G3P2H0   A0A3Q3JUL3   A0A3Q2PLH1   A0A2S2PBH9   H2LS59   A0A087Y0C3   I3K701   I3K702   A0A3Q1G2I5   A0A147A9H4   A0A2U9BSQ7   A0A3Q2FHA8   A0A3P9CEN5   A0A3Q1HWQ0   A0A3B3B8B5   A0A1B6ISZ2   A0A3P9QGC7   A0A3P8R9T4   A0A3B4GG59   A0A3N0Z0K3   A0A3B3XTA9   A0A3B3XSY1   A0A2U9BS92   A0A3Q0SIH0   C4WWX2   A0A2R5LHV2   A0A3Q3XIP8   A0A3P8U6V0   A0A060WH90   A0A3B1KAJ0   E9QG26   A0A3Q4H1K2   A0A3Q2UZE1   A3QK41   A0A2P2I833   A0A3Q1BN59   A0A3P8Y769   A0A0P4WCI8   G3R2E5   A0A2R9AWD5   H2Q6U0   Q6NS38   G3RZ41   A0A3B4CST3   A0A2G8JT43   A0A2K5HSH0   Q4RSX1   A0A2K6BD08   A0A2K6MNL8   A0A2K6PVN6   A0A2K5KWI2   A0A2K5XJD9   A0A096MYW1   G7PI61   F7DWK4   A0A2K5DI25   A0A2J8XLJ4   A0A3P8VI60   A0A3Q2YVB0  

PDB

3RZM     E-value=3.99349e-24,     Score=269

KEGG

101737561    K10859 DNA oxidative demethylase [EC:1.14.11.33] | (RefSeq) alpha-ketoglutarate-dependent dioxygenas

GO

GO:0016491   GO:0051213   GO:0051747   GO:0006307   GO:0005634   GO:0008198   GO:0035511   GO:0043734   GO:0008168   GO:0005654   GO:0015630   GO:0080111   GO:0070989   GO:0103053  

Subcellular location

Nucleus  

Length:

121

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

1.37783

Exp number, first 60 AAs:

1.37464

Total prob of N-in:

0.19904

outside

1  -  121

 

 

Pi

18.04247

Theta

17.639114

Tajima's D

-0.197785

CLR

0.003427

CSRT

0.322033898305085

Interpretation

Uncertain