Gene

KWMTBOMO05993  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA002818

Annotation

PREDICTED:_thioredoxin_reductase_1?_mitochondrial_isoform_X2_[Bombyx_mori]

Location

Bomo_Chr10(+):15898568-15912411      View in EpiBrowser

Full name

Thioredoxin reductase 1, mitochondrial      

Location in the cell

Cytoplasmic   Reliability : 2.58

CDS

ATGGCACCTGTCGCAGGAACATACGACTATGATCTGGCTGTAATCGGAGGTGGCTCCGGTGGACTGGCATGCGCCAAGGAGGCTGTGAACTTGGGTGCTAAAGTGACTGTGTTGGACTACGTCACACCCTCACCGCAAGGCACCAAGTGGGGCCTGGGAGGAACCTGTGTTAATGTAGGATGTATTCCGAAGAAGCTGATGCATCAGGCTGCTTTACTTGGAGAGAGCATACATGAAGCGGTTGCATACGGTTGGGAGGTGCCCTCACTGGACGCGATCAAGATAAACTGGCCAGCGCTAACTGAAGCTGTACAGAATCACATAAAATCTGTGAACTGGGTGACCAGGGTTGACCTGAGAGAGAAGAAAATAGATTACGTTAACGGTCTGGGTGAGTTTAAGGACGCCCACACGCTGATAGCGACCTTGAAGAATGGATCGAAGAAGGAGATCACGGCTAAGAATATTGTGATCGCCGTGGGCGGTAGGCCGCACTACCCGGACATTCCTGGGGCGGTCGAGTACTGCATCAGCAGTGACGATATCTTCAGTTTAGGTCATCCCCCCGGGAAGACCCTGGTCGTTGGAGCTGGATACATCGGCTTAGAATGCGCCGGATTTTTGAACTCTTTGGGTTACCCGGCCACCGTGCTGGTCCGATCGGTGCCACTCCGCGGCTTCGACCAGCAAATGGCCCAGGCGGTAACCAGCGAGATGGAACAGAAGGGAGTCGTATTCCACAACAAGTGCGTACCGCTGTCCGTGGAGAAGCTGGAGACCGGTCAGTTGAAGGCGCGCTGGCAGAACACGGAGACCCAAGAGCGGGGTGAGGATGTGTTCGATACGGTGCTGATGGCGACCGGCCGGTACGCCCTCACCAAAACCCTCAACCTCGAAGCGGCAGGAGTGACGTGTGTATCAAACAGCGGGAAGATCATAGCTGAAACTGAACAGACCAACGTCTCTAACATATACGCGGTGGGCGACGTGTTGGAGGGGAAGCCTGAGCTGACCCCCGTGGCCATCCACGCGGGCCGGCTGCTGGCGCGCCGGATGTTCGCCGGGGCCACGCAGCCCATGGACTACGACAACGTGGCCACCACCGTGTTCACGCCGTTAGAGTACGGCTGCGTCGGGCTAAGCGAGGAGACCGCCCTGGCCAGGCACGGCGCCGACAAGGTGGAGGTGTACCACGCGTTCTACAAACCGACGGAGTTCTTCATACCGCAGAGGAACATCCGCAACTGCTACCTGAAGGCCGTGGCGCTCCGCGAGGCGCCGCAGCGCATCCTCGGGCTCCACTTTGTGGGGCCGGTCGCCGGCGAGGTCATACAGGGCTTCGCAGCTGCCGTCAAATGCGGCATAACTATGGAGCAATTGATGAACACAGTCGGCATTCACCCCACGGTGGCCGAGGAGTTCACCAGACTCAACATCACCAAGCGCTCCGGCAAGGACCCCAACCCTGCGTCGTGCTGCAGCTAG

Protein

MAPVAGTYDYDLAVIGGGSGGLACAKEAVNLGAKVTVLDYVTPSPQGTKWGLGGTCVNVGCIPKKLMHQAALLGESIHEAVAYGWEVPSLDAIKINWPALTEAVQNHIKSVNWVTRVDLREKKIDYVNGLGEFKDAHTLIATLKNGSKKEITAKNIVIAVGGRPHYPDIPGAVEYCISSDDIFSLGHPPGKTLVVGAGYIGLECAGFLNSLGYPATVLVRSVPLRGFDQQMAQAVTSEMEQKGVVFHNKCVPLSVEKLETGQLKARWQNTETQERGEDVFDTVLMATGRYALTKTLNLEAAGVTCVSNSGKIIAETEQTNVSNIYAVGDVLEGKPELTPVAIHAGRLLARRMFAGATQPMDYDNVATTVFTPLEYGCVGLSEETALARHGADKVEVYHAFYKPTEFFIPQRNIRNCYLKAVALREAPQRILGLHFVGPVAGEVIQGFAAAVKCGITMEQLMNTVGIHPTVAEEFTRLNITKRSGKDPNPASCCS

Description

Thioredoxin system is a major player in glutathione metabolism, due to the demonstrated absence of a glutathione reductase. Functionally interacts with the Sod/Cat reactive oxidation species (ROS) defense system and thereby has a role in preadult development and life span. Lack of a glutathione reductase suggests antioxidant defense in Drosophila, and probably in related insects, differs fundamentally from that in other organisms.

Catalytic Activity

[thioredoxin]-dithiol + NADP(+) = [thioredoxin]-disulfide + H(+) + NADPH

Cofactor

FAD

Biophysicochemical Properties

6.5 uM for NADPH (at pH 7.4)
1 uM for NADPH (at pH 7.4, 2 mM EDTA, 100 mM KPO(4))
1 uM for NADPH (isoform B at pH 7.4, 2 mM EDTA, 100 mM KPO(4))
7.0 uM for dhd (at pH 7.4, 200 uM NADPH, 100 mM KPO(4))
141 uM for dhd (at pH 7.0, 0.15 mM NADPH, 1 mM EDTA, 1 mg/ml insulin, 50 mM KPO(4), 25 degrees Celsius)
7 uM for dhd (at pH 7.4, 2 mM EDTA, 100 mM KPO(4))
19 uM for dhd (isoform B at pH 7.4, 2 mM EDTA, 100 mM KPO(4))
5.9 uM for Trx-2 (at pH 7.4, 100 uM NADPH, 2 mM EDTA, 100 mM KPO(4))
310 uM for 5,5'-dithiobis-2-nitrobenzoic acid (DTNB) (at pH 7.4, 100 uM NADPH, 100 mM KPO(4))
0.17 mM for DTNB (at pH 7.0, 0.2 mM NADPH, 10 mM EDTA, 100 mM KPO(4), 25 degrees Celsius)
380 uM for DTNB (at pH 7.4, 2 mM EDTA, 100 mM KPO(4))
410 uM for DTNB (isoform B at pH 7.4, 2 mM EDTA, 100 mM KPO(4))
675 uM for methylseleninate (100 uM NADPH)

Subunit

Homodimer.

Miscellaneous

The active site is a redox-active disulfide bond.

Similarity

Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Keywords

3D-structure   Alternative initiation   Alternative splicing   Complete proteome   Cytoplasm   Disulfide bond   FAD   Flavoprotein   Mitochondrion   NADP   Nucleotide-binding   Oxidoreductase   Redox-active center   Reference proteome   Transit peptide  

Feature

chain  Thioredoxin reductase 1, mitochondrial
splice variant  In isoform A and isoform D.

Uniprot

H9IZY3   S4PGR5   A0A2H1VTD3   A0A0A7RB97   A0A194QSN1   A0A3S2LW17   + More
A0A212EHM0   A0A2A4JMR9   W8BVK8   A0A1L8EFX8   A0A0A1WJ37   A0A034VC07   A0A1L8DZH0   A0A1L8EFM1   A0A1I8P8B7   A0A1I8P8E9   A0A2J7PD15   A0A1I8P889   A0A2J7PD09   A0A1L8E9N4   T1PE19   A0A034V6Y5   P91884   A0A034VAF2   A0A1I8P866   W5JC17   A0A182Y7I3   A0A336MJC3   A0A336L8M5   A0A1L8DZG2   A0A3B0KKV3   A0A2M4CIZ1   A0A1W4XAK5   A0A1W4X9D2   Q29FR6   A0A2M4CHN3   A0A2M4CHN1   A0A2M4CHX9   A0A2M3Z590   W5JM65   A0A182PQ49   A0A182RV39   J3JWU4   A0A084VFN0   Q86GB3   A0A2M4AB12   A0A2M4A9Z4   Q86GB2   A0A0N8P0L5   Q8MZM6   A0A0R3P666   A0A2M4AAC2   A0A2M4BJZ2   A0A182UQM2   A0A067RB75   A0A182IA68   A0A182FJY4   A0A2M4BK59   A0A1W4VX70   A0A2M4ADP1   A0A182XLQ9   A0A182NVI1   A0A182W4R7   U5EQ42   A0A2M4AD74   A0A182F127   A0A182MCK5   A0A182QER2   A0A182U8H8   A0A2M4BL19   B4Q0D5   A0A182KEH4   A0A2M4ABX6   B3NX17   B3MXU2   A0A0L0BM47   A0A0Q9WU01   A0A2M3ZGC9   A0A2M4AIK6   A0A2M3Z2G9   A0A0K8TUD9   A0A1W4VJ61   P91938-2   A0A1W4VWE3   A0A0R1EHY5   A0A0R1EBL0   A0A1J1IYM2   A0A1B6CAT4   A0A0P4VIW8   A0A0Q5T6X3   D6WDX1   B4NDH3   A0A0Q5TAX5   A0A1B6CT38   A0A0V0G4M4   A0A182J4F6   A0A1B6BXA1   A0A069DV52   A0A1A9WL68   Q17GT5  

EC Number

1.8.1.9

Pubmed

19121390    23622113    26502992    26354079    22118469    24495485    + More
25830018    25348373    25315136    20920257    23761445    25244985    15632085    17994087    22516182    23537049    24438588    12364791    14747013    17210077    24845553    17550304    26108605    26369729    9056265    11158675    10731132    12537572    12537569    11525742    11782468    11796729    11877442    18211101    18991392    17385893    27129103    18362917    19820115    26334808    17510324   

EMBL

BABH01018954    GAIX01003537    JAA89023.1    ODYU01004342    SOQ44111.1    KM658552    + More
AJA32127.1    KQ461175    KPJ07990.1    RSAL01000002    RVE54960.1    AGBW02014864    OWR40977.1    NWSH01001058    PCG72884.1    GAMC01005604    GAMC01005602    JAC00954.1    GFDG01001316    JAV17483.1    GBXI01015894    JAC98397.1    GAKP01019864    GAKP01019863    JAC39088.1    GFDF01002258    JAV11826.1    GFDG01001321    JAV17478.1    NEVH01026394    PNF14225.1    PNF14223.1    GFDG01003395    JAV15404.1    KA646966    AFP61595.1    GAKP01019866    JAC39086.1    U88187    AAC69637.1    GAKP01019865    JAC39087.1    ADMH02001645    ETN61621.1    UFQT01001105    SSX28933.1    UFQS01002506    UFQT01002506    SSX14186.1    SSX33602.1    GFDF01002260    JAV11824.1    OUUW01000018    SPP89210.1    GGFL01000670    MBW64848.1    CH379065    EAL31513.2    GGFL01000672    MBW64850.1    GGFL01000669    MBW64847.1    GGFL01000671    MBW64849.1    GGFM01002929    MBW23680.1    ADMH02000725    ETN65241.1    APGK01058933    BT127712    KB741292    AEE62674.1    ENN70347.1    ATLV01012450    KE524793    KFB36774.1    AJ549084    AAAB01008846    CAD70158.1    EAA06298.3    GGFK01004655    MBW37976.1    GGFK01004286    MBW37607.1    AJ549085    CAD70159.1    EAU77244.1    CH902630    KPU77460.1    AJ459821    CAD30858.1    EGK96416.1    KRT07247.1    GGFK01004433    MBW37754.1    GGFJ01004239    MBW53380.1    KK852620    KDR20063.1    APCN01001127    GGFJ01004271    MBW53412.1    GGFK01005585    MBW38906.1    GANO01003434    JAB56437.1    GGFK01005410    MBW38731.1    AXCM01000581    AXCN02002153    GGFJ01004626    MBW53767.1    CM000162    EDX02272.1    GGFK01004939    MBW38260.1    CH954180    EDV46846.1    EDV38557.2    JRES01001679    KNC20998.1    CH964239    KRF99678.1    GGFM01006876    MBW27627.1    GGFK01007292    MBW40613.1    GGFM01001968    MBW22719.1    GDAI01000048    JAI17555.1    U81995    AF301145    AF301144    AE014298    BT003266    BT025070    AY051643    KRK06682.1    KRK06681.1    CVRI01000063    CRL04794.1    GEDC01026784    JAS10514.1    GDKW01002590    JAI54005.1    KQS30144.1    KQ971323    EEZ99907.2    EDW82879.1    KQS30145.1    GEDC01020741    GEDC01000988    JAS16557.1    JAS36310.1    GECL01003116    JAP03008.1    GEDC01031386    JAS05912.1    GBGD01001193    JAC87696.1    CH477256    EAT45853.1   

Proteomes

UP000005204    UP000053240    UP000283053    UP000007151    UP000218220    UP000095300    + More
UP000235965    UP000095301    UP000000673    UP000076408    UP000268350    UP000192223    UP000001819    UP000075885    UP000075900    UP000019118    UP000030765    UP000007062    UP000007801    UP000075903    UP000027135    UP000075840    UP000069272    UP000192221    UP000076407    UP000075884    UP000075920    UP000075883    UP000075886    UP000075902    UP000002282    UP000075881    UP000008711    UP000037069    UP000007798    UP000000803    UP000183832    UP000007266    UP000075880    UP000091820    UP000008820   

PRIDE

H9IZY3    P91938   

Pfam

PF02852   Pyr_redox_dim        + More
PF07992   Pyr_redox_2

Interpro

IPR023753   FAD/NAD-binding_dom        + More
IPR036188   FAD/NAD-bd_sf       
IPR004099   Pyr_nucl-diS_OxRdtase_dimer       
IPR001100   Pyr_nuc-diS_OxRdtase       
IPR006338   Thioredoxin/glutathione_Rdtase       
IPR016156   FAD/NAD-linked_Rdtase_dimer_sf       
IPR012999   Pyr_OxRdtase_I_AS       

SUPFAM

SSF55424   SSF55424        + More
SSF51905   SSF51905       

Gene 3D

3.50.50.60     3.30.390.30    

ProteinModelPortal

H9IZY3   S4PGR5   A0A2H1VTD3   A0A0A7RB97   A0A194QSN1   A0A3S2LW17   + More
A0A212EHM0   A0A2A4JMR9   W8BVK8   A0A1L8EFX8   A0A0A1WJ37   A0A034VC07   A0A1L8DZH0   A0A1L8EFM1   A0A1I8P8B7   A0A1I8P8E9   A0A2J7PD15   A0A1I8P889   A0A2J7PD09   A0A1L8E9N4   T1PE19   A0A034V6Y5   P91884   A0A034VAF2   A0A1I8P866   W5JC17   A0A182Y7I3   A0A336MJC3   A0A336L8M5   A0A1L8DZG2   A0A3B0KKV3   A0A2M4CIZ1   A0A1W4XAK5   A0A1W4X9D2   Q29FR6   A0A2M4CHN3   A0A2M4CHN1   A0A2M4CHX9   A0A2M3Z590   W5JM65   A0A182PQ49   A0A182RV39   J3JWU4   A0A084VFN0   Q86GB3   A0A2M4AB12   A0A2M4A9Z4   Q86GB2   A0A0N8P0L5   Q8MZM6   A0A0R3P666   A0A2M4AAC2   A0A2M4BJZ2   A0A182UQM2   A0A067RB75   A0A182IA68   A0A182FJY4   A0A2M4BK59   A0A1W4VX70   A0A2M4ADP1   A0A182XLQ9   A0A182NVI1   A0A182W4R7   U5EQ42   A0A2M4AD74   A0A182F127   A0A182MCK5   A0A182QER2   A0A182U8H8   A0A2M4BL19   B4Q0D5   A0A182KEH4   A0A2M4ABX6   B3NX17   B3MXU2   A0A0L0BM47   A0A0Q9WU01   A0A2M3ZGC9   A0A2M4AIK6   A0A2M3Z2G9   A0A0K8TUD9   A0A1W4VJ61   P91938-2   A0A1W4VWE3   A0A0R1EHY5   A0A0R1EBL0   A0A1J1IYM2   A0A1B6CAT4   A0A0P4VIW8   A0A0Q5T6X3   D6WDX1   B4NDH3   A0A0Q5TAX5   A0A1B6CT38   A0A0V0G4M4   A0A182J4F6   A0A1B6BXA1   A0A069DV52   A0A1A9WL68   Q17GT5  

PDB

2NVK     E-value=0,     Score=1770

KEGG

101738027    K22182 thioredoxin reductase (NADPH) [EC:1.8.1.9] | (RefSeq) thioredoxin reductase 1, mitochondrial

PATHWAY

00450    Selenocompound metabolism - Bombyx mori (domestic silkworm)

GO

GO:0005623   GO:0050660   GO:0004791   GO:0045454   GO:0009055   GO:0016491   GO:0005737   GO:0005739   GO:0001666   GO:0004362   GO:0042803   GO:0008340   GO:0005829   GO:0016209   GO:0016668   GO:0006508   GO:0005622   GO:0007264   GO:0008026   GO:0016597   GO:0055114  

Subcellular location

Mitochondrion  
Cytoplasm  

Length:

494

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.32592

Exp number, first 60 AAs:

0.02269

Total prob of N-in:

0.00714

outside

1  -  494

 

 

Pi

16.29712

Theta

18.769955

Tajima's D

-0.637385

CLR

2.37118

CSRT

0.210589470526474

Interpretation

Uncertain