Gene

KWMTBOMO05987  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA002814

Annotation

dolichyl-diphosphooligosaccharide_protein_glycotransferase_precursor_[Bombyx_mori]

Location

Bomo_Chr10(+):15804728-15809937      View in EpiBrowser

Full name

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit      

Alternative Name

DmOST50
OST5OP

Location in the cell

PlasmaMembrane   Reliability : 1.199

CDS

ATGAAAACCACGATAATTTTAGGGACATTGATCACTTTTTTAACGCTCGTTTGCGCGGATCATGAAACTTTAGTTCTAATAGATAACTTAAATATCAAAGAAACGCATTCACAATTCTTCAAATCATTACAAGAACGCGGCTATGGCTTAACATTCAAACTTGCGGATGATGCAAATCTGGTCCTGTCAAAATACGGAGAATATTTATACAAGAATCTCATAGTATTCGCTCCTTCCGTGCTCGAGTTTGGTGGACAGGTAGACAGTGAGGCCATAACCAAATTCATAGACGATGGCGGAAACCTTCTTATGGCTGGTAACGCCGCGGCCGGAGACGTATATAGAGAAATCGCTTCAGAATGTGGATTTGAGATGGACGAGGAATCTGCAGCAGTCATAGACCACTTCAACTATGATGTGACTGATGAGGGAGACCACACTCGCATTGTAGTATCACCTAAGAACCTAATCAAGGCTCCCACAATTGTAGGAGAACAGAATACACAGCCTCTGCTATTTGAAGGCACTGGACTTATTGTGGACAAGGATAACAGCTTGGTTTTGCCTATTCTATCAGCTGATTCGACTGCCTACAGTTACAACCCTAAGAGTCAGGTGAAAGAGTACCCCCATGCAGTTGGCCGTAAGACAGTACTGATTGCAGCTCTGCAAGCAAGAAACAATGCTCGTATTGTTTTCAGTGGATCGTTATTCTTCTTCTCTGATGAGGCCTTCAATTCTCCTGTTACAAAAGTACATGGTGACAAAACAAAGTCAGATGTATCAGGCAACAAGATTCTCGCGATTCGCCTTACTGAATGGGTGTTTGGTGAGCGTGGGCGTTTACGCGTGCGCAATGTCGACCATCACAGACAAGGAGAGAAGGAACCGTCCAGTGCCTATACCATTACAGACACTGTTGTATACAGAATTGAAATTGAAGAGCTAAAGAATGGCAAATGGCAGCCTTTCGAAGCAAACGATGTGCAGCTAGAGTTTGTGCGAATCGACCCTTTTATACGCACAACTTTACAAAAGAAACCCAATGGCGTCTATGAGGCTGTCTTCAAAGTTCCAGATGTCTGGGGTGTTTATCAGTTCAAAGTGGATTACGACAGGGTCGGGTATACAAGACTTTATCACTCAACACAGGTGTCAGTCCGCCCACTACAACACACACAATATGAGCGCTTCATACCGAGCGCCTACCCATACTACGTTAGTTCGTTCTCAATGATGATCGGAGTGTTCTTATTTTCATTTGTTTTCCTGTATTACAAAGAGGATCAACCTAAAACGAAGAGCGAGTAA

Protein

MKTTIILGTLITFLTLVCADHETLVLIDNLNIKETHSQFFKSLQERGYGLTFKLADDANLVLSKYGEYLYKNLIVFAPSVLEFGGQVDSEAITKFIDDGGNLLMAGNAAAGDVYREIASECGFEMDEESAAVIDHFNYDVTDEGDHTRIVVSPKNLIKAPTIVGEQNTQPLLFEGTGLIVDKDNSLVLPILSADSTAYSYNPKSQVKEYPHAVGRKTVLIAALQARNNARIVFSGSLFFFSDEAFNSPVTKVHGDKTKSDVSGNKILAIRLTEWVFGERGRLRVRNVDHHRQGEKEPSSAYTITDTVVYRIEIEELKNGKWQPFEANDVQLEFVRIDPFIRTTLQKKPNGVYEAVFKVPDVWGVYQFKVDYDRVGYTRLYHSTQVSVRPLQHTQYERFIPSAYPYYVSSFSMMIGVFLFSFVFLYYKEDQPKTKSE

Description

Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER).
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity (By similarity). Required for the assembly of both SST3A- and SS3B-containing OST complexes (By similarity).

Subunit

Component of the oligosaccharyltransferase (OST) complex.

Similarity

Belongs to the DDOST 48 kDa subunit family.

Keywords

Complete proteome   Endoplasmic reticulum   Membrane   Reference proteome   Signal   Transmembrane   Transmembrane helix  

Feature

chain  Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit

Uniprot

A0FDQ5   H9IZX9   A0A2A4JLB9   A0A2H1WX14   I4DK30   A0A194Q3P6   + More
I4DRF8   A0A194QRM6   A0A212FCC4   A0A1E1W904   S4PAG1   A0A3S2NMT3   A0A0L7LEF1   A0A2J7PJI1   V5I952   A0A1S4F0X0   A0A067RD92   Q1HQY4   B0WE14   Q17J35   A0A023ESF1   A0A023ETI7   A0A182GZ84   A0A023ESB2   A0A023EUC7   A0A023ESE0   A0A232EHP0   A0A2M4BML0   A0A2M3Z0U0   A0A182FEF1   T1DQ01   A0A2M3Z1B1   A0A2M4BMD0   A0A0K8UV07   A0A034WTU4   A0A0C9QGL1   A0A0K8VDP9   A0A0L0C2J1   A0A0A1X2T1   W5JDA9   A0A0T6B727   A0A2M4A310   A0A2M4A357   A0A2M4A2Z9   R4FP71   A0A2M4A360   A0A023FXA2   A0A182QHG3   A0A1E1XUM9   A0A131YPE1   U5ETM0   W8C5U0   A0A023FND2   L7M579   A0A1Y1M4V4   A0A1B0GK31   A0A224YLK5   A0A3L8DAL9   A0A1L8E214   A0A026X209   A0A023GPG5   K7ITE8   A0A3B0KPJ1   B4MG86   Q29GX7   B4H2F2   B3MZQ1   A0A1Q3FF06   A0A0M4EVG4   T1PDN4   A0A1I8MCC7   A0A1W4UDK9   A0A1L8EDS7   A0A1E1X841   B4R6U5   A0A1L8EDF5   B4IJR8   B4L5P4   A0A087ZSQ6   A0A1I8PTK8   A0A1A9W938   A0A0A9ZDN9   B4PZW9   Q24319   F4X2E1   B3NUG9   A0A154PD93   A0A1B6EYK8   B4JLA5   E2AC67   A0A195CS14   A0A1B6D8E2   A0A2P8XQA1   A0A1B6J273   A0A023ERT7   E9IX39   A0A0L7QSL5   A0A151WJZ2   A0A224XCL9   A0A182MVG7  

Pubmed

19121390    22651552    26354079    22118469    23622113    26227816    + More
24845553    17204158    17510324    24945155    26483478    28648823    25348373    26108605    25830018    20920257    23761445    29209593    26830274    24495485    25576852    28004739    28797301    30249741    24508170    20075255    17994087    15632085    25315136    28503490    25401762    26823975    17550304    7607543    10731132    12537572    7557364    21719571    20798317    29403074    21282665   

EMBL

DQ985595    ABJ97185.1    BABH01018964    BABH01018965    BABH01018966    NWSH01001058    + More
PCG72875.1    ODYU01011649    SOQ57532.1    AK401648    BAM18270.1    KQ459472    KPJ00157.1    AK405099    BAM20498.1    KQ461175    KPJ07984.1    AGBW02009199    OWR51396.1    GDQN01007619    JAT83435.1    GAIX01003279    JAA89281.1    RSAL01000002    RVE54965.1    JTDY01001444    KOB73847.1    NEVH01024947    PNF16497.1    GALX01003540    JAB64926.1    KK852575    KDR21004.1    DQ440310    ABF18343.1    DS231903    EDS45144.1    CH477235    EAT46676.1    GAPW01001421    JAC12177.1    GAPW01001422    JAC12176.1    JXUM01099006    KQ564462    KXJ72237.1    GAPW01001461    JAC12137.1    GAPW01001559    JAC12039.1    GAPW01001423    JAC12175.1    NNAY01004460    OXU17865.1    GGFJ01005121    MBW54262.1    GGFM01001307    MBW22058.1    GAMD01001515    JAB00076.1    GGFM01001544    MBW22295.1    GGFJ01005064    MBW54205.1    GDHF01021807    JAI30507.1    GAKP01001215    JAC57737.1    GBYB01002614    JAG72381.1    GDHF01015250    JAI37064.1    JRES01000984    KNC26471.1    GBXI01008890    JAD05402.1    ADMH02001563    ETN62061.1    LJIG01009468    KRT83019.1    GGFK01001872    MBW35193.1    GGFK01001878    MBW35199.1    GGFK01001862    MBW35183.1    ACPB03019847    GAHY01001029    JAA76481.1    GGFK01001864    MBW35185.1    GBBL01001163    JAC26157.1    AXCN02000888    GFAA01000783    JAU02652.1    GEDV01008786    JAP79771.1    GANO01002718    JAB57153.1    GAMC01004674    JAC01882.1    GBBK01001713    JAC22769.1    GACK01006731    JAA58303.1    GEZM01041249    GEZM01041248    JAV80541.1    AJWK01022522    GFPF01007371    MAA18517.1    QOIP01000011    RLU16968.1    GFDF01001323    JAV12761.1    KK107021    EZA62350.1    GBBM01000093    JAC35325.1    AAZX01006673    OUUW01000015    SPP88539.1    CH940672    EDW57409.1    CH379064    EAL31982.1    CH479204    EDW30519.1    CH902635    EDV33852.1    GFDL01008920    JAV26125.1    CP012528    ALC49260.1    KA646804    AFP61433.1    GFDG01002040    JAV16759.1    GFAC01003972    JAT95216.1    CM000366    EDX17475.1    GFDG01002042    JAV16757.1    CH480849    EDW51276.1    CH933811    EDW06503.1    GBHO01000217    GDHC01002492    JAG43387.1    JAQ16137.1    CM000162    EDX02175.1    X81999    AE014298    BT010050    X81207    GL888578    EGI59366.1    CH954180    EDV46292.1    KQ434868    KZC09180.1    GECZ01026707    JAS43062.1    CH916370    EDW00358.1    GL438428    EFN68961.1    KQ977329    KYN03528.1    GEDC01015453    JAS21845.1    PYGN01001545    PSN34155.1    GECU01014438    JAS93268.1    GAPW01001668    JAC11930.1    GL766616    EFZ14880.1    KQ414756    KOC61643.1    KQ983028    KYQ48154.1    GFTR01006250    JAW10176.1    AXCM01000418   

Proteomes

UP000005204    UP000218220    UP000053268    UP000053240    UP000007151    UP000283053    + More
UP000037510    UP000235965    UP000027135    UP000002320    UP000008820    UP000069940    UP000249989    UP000215335    UP000069272    UP000037069    UP000000673    UP000015103    UP000075886    UP000092461    UP000279307    UP000053097    UP000002358    UP000268350    UP000008792    UP000001819    UP000008744    UP000007801    UP000092553    UP000095301    UP000192221    UP000000304    UP000001292    UP000009192    UP000005203    UP000095300    UP000091820    UP000002282    UP000000803    UP000007755    UP000008711    UP000076502    UP000001070    UP000000311    UP000078542    UP000245037    UP000053825    UP000075809    UP000075883   

PRIDE

A0FDQ5    H9IZX9    L7M579    Q24319   

Pfam

PF03345   DDOST_48kD

Interpro

IPR005013   DDOST_48_kDa_subunit       

ProteinModelPortal

A0FDQ5   H9IZX9   A0A2A4JLB9   A0A2H1WX14   I4DK30   A0A194Q3P6   + More
I4DRF8   A0A194QRM6   A0A212FCC4   A0A1E1W904   S4PAG1   A0A3S2NMT3   A0A0L7LEF1   A0A2J7PJI1   V5I952   A0A1S4F0X0   A0A067RD92   Q1HQY4   B0WE14   Q17J35   A0A023ESF1   A0A023ETI7   A0A182GZ84   A0A023ESB2   A0A023EUC7   A0A023ESE0   A0A232EHP0   A0A2M4BML0   A0A2M3Z0U0   A0A182FEF1   T1DQ01   A0A2M3Z1B1   A0A2M4BMD0   A0A0K8UV07   A0A034WTU4   A0A0C9QGL1   A0A0K8VDP9   A0A0L0C2J1   A0A0A1X2T1   W5JDA9   A0A0T6B727   A0A2M4A310   A0A2M4A357   A0A2M4A2Z9   R4FP71   A0A2M4A360   A0A023FXA2   A0A182QHG3   A0A1E1XUM9   A0A131YPE1   U5ETM0   W8C5U0   A0A023FND2   L7M579   A0A1Y1M4V4   A0A1B0GK31   A0A224YLK5   A0A3L8DAL9   A0A1L8E214   A0A026X209   A0A023GPG5   K7ITE8   A0A3B0KPJ1   B4MG86   Q29GX7   B4H2F2   B3MZQ1   A0A1Q3FF06   A0A0M4EVG4   T1PDN4   A0A1I8MCC7   A0A1W4UDK9   A0A1L8EDS7   A0A1E1X841   B4R6U5   A0A1L8EDF5   B4IJR8   B4L5P4   A0A087ZSQ6   A0A1I8PTK8   A0A1A9W938   A0A0A9ZDN9   B4PZW9   Q24319   F4X2E1   B3NUG9   A0A154PD93   A0A1B6EYK8   B4JLA5   E2AC67   A0A195CS14   A0A1B6D8E2   A0A2P8XQA1   A0A1B6J273   A0A023ERT7   E9IX39   A0A0L7QSL5   A0A151WJZ2   A0A224XCL9   A0A182MVG7  

PDB

6EZN     E-value=2.94478e-19,     Score=234

KEGG

778504    K12670 oligosaccharyltransferase complex subunit beta | (RefSeq) dolichyl-diphosphooligosaccharide p

PATHWAY

00510    N-Glycan biosynthesis - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)
04141    Protein processing in endoplasmic reticulum - Bombyx mori (domestic silkworm)

GO

GO:0005789   GO:0016021   GO:0018279   GO:0016740   GO:0016757   GO:0034389   GO:0007030   GO:0012505   GO:0006412   GO:0005622   GO:0008270   GO:0006508   GO:0007264   GO:0008026   GO:0016597  

PANTHER

PTHR10830  

Subcellular location

Endoplasmic reticulum membrane  

SignalP

Position:   1 - 19,         Likelihood:  0.977329

 

 

Length:

436

Number of predicted TMHs:

1

Exp number of AAs in TMHs:

25.7222

Exp number, first 60 AAs:

3.18176

Total prob of N-in:

0.14045

outside

1  -  401

TMhelix

402  -  424

inside

425  -  436

 

 

Pi

23.007447

Theta

24.109944

Tajima's D

-0.487886

CLR

1.057635

CSRT

0.252287385630718

Interpretation

Uncertain