Gene

KWMTBOMO05973  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA002895

Annotation

PREDICTED:_ATP-dependent_RNA_helicase_DHX8_[Papilio_xuthus]

Location

Bomo_Chr10(-):15654142-15665247      View in EpiBrowser

Full name

ATP-dependent RNA helicase DHX8      

Alternative Name

Peanuts

Location in the cell

Nuclear   Reliability : 4.281

CDS

ATGGACGAAGTCACTAAATTAGAGCATCTTTCGCTCGTGTCTAAAATATGCACCGAGCTAGACAACCATTTAGGTTTGAACGACAAGGATTTAGCGGAATTCATCATAGATTTGGCTGATAAGAATCCAACATTTGAAACTTTTAAAGTAGCATTGGTTGAAAATGGGGCTGAATTTTCTGATTCCTTCATGACAAATCTACTCCGTATAATACAGCACATGAAACCTGTGAATACATCAACATTGATTAAAAATATTGCCGAAACTGAAAGTGAAAATCCTTTGGCAAAAAAGTTTCCTGGCTTGGCCATACCAAATGATAAACCTCAATCTTTTTCTGATGAAAGTAGTGATGATGAAAAAGACAAAAAGAGAATAACAGCCAAAGACTTATTTACAGAAGAAATTAAACCAAGTTCTACTGATAATGTTGTTGATTTGGCGATGGCTGAATTGGAAGCTTTAGCACCTTCCAATTCTACAACAAATGAACTTCTTATAGGAAAAGAACCCCTAAAAATTGAGAGTAGTAGAAATAGTGATCACAAAGAAAAGAATAAATCAGAAGAATCTAGAGATAAAGAGAGTAGGAGTCGAAATCCTAAAAGAAGCAGAAGTAGGGAACGTAAAAGAAGCAGAAGTACACAGAACACAGATAAATATAAACTAGAAGAATCTCGAGATAGAAAAAGGCATAGTAGAAGTCGAGATCGTAAAAGAAGTAGGAGCCGAGATAGAAAAAGAAGTAGGAGCCGAGATCGTAGAAGAAGTAGGAGTAGAGATTATAAAAGAAGTAGAAGTAGGGAACGTAAAAGAAGTAGAAGTAAAGGTAAGAGAAGTCGTAGCCGGGATAGGAGAAGCAAAAGTCAAGACAGAGACCGTAGAAGAAGATCACACAGTCGACATCGTCACAGATCCAGGTCTAGGGACAGGCGTAGATCTTACTCGAAAAGTAGAAGATCAGTATCAGGTGGCAGAAGATCTCGTTCAAGAAATAGAGAAGAAAAATTTGATTATGGCAAGAAAAGCAGAAAACGCAGCCCTGAAGTAGAATTGAACGATGATCCTGAACCAGGAAAAATATACAGTGGCCGCGTTGCAAACATTGTACCATTCGGCTGTTTTGTTCAAATTGAAGGAGTCAGAAAAAGGTGGGAAGGATTGGTGCATATCTCACAGCTGCGAGCTGAGGGTCGTGTCACCAATGTATCTGATGTTGTCAATAGAGGTGATAAAGTTAAGGTCAAAGTAATCTCAATAACAGGACAGAAAGTTTCTCTCACAATGAAGGATGTTTGTCAAGAGACCGGGAAGGATCTCAATCCAGCATCCCATGCTCACCTGGAAGCAGAACGTGAAGGCCGAAACCCTGATCGCCCTCCACCAGCAGCAACTGTGTTAGCCGGACTCCAAGGTGGAATAGACCCTGATGAGGATTCTAGTAGGAAGAGGGTGACAAGGATATCCAGTCCTGAGAGATGGGAGATCAAACAGATGATTTCATCAGGCGTAATTGATAAGAGTGAATTACCAGATTTTGATGAGGAGACTGGTTTACTTCCAAAAGATGAAGATGGCGAGGCAGACATTGAAATAGAGCTAGTAGAAGAAGAACCTCCTTTCCTCCAAGGTCATGGCAGAGCCCTTCACGATCTTTCTCCAGTAAGGATAGTTAAGAATCCCGATGGCTCCCTTGCACAAGCAGCCATGATGCAGTCCGCATTGGCCAAAGAGAGGAGAGAGCAGAAGATGATCCAACGGGAGCAGGAAATGGACAGCGTACCTACTGGTCTAAATAAAAATTGGATAGATCCGCTACCTGAGTCCGAGGGTCGAACGTTAGCGGCCAACATGCGAGGCGCGGGCGTGGTCCCTCAAGACTTGCCAGAATGGAAGAAACACGTCATCGGTGGAAAAAAATCCTCATTTGGAAAGAAAACGAATTTATCCCTGCTCGAGCAGAGACAGTCGCTGCCCATATACAAGCTTAGAGATGAACTAACTAAGGCCGTGTCCGACAACCAGATCCTGATCGTGATCGGCGAGACGGGGTCGGGCAAGACGACGCAGATAACGCAGTACGTGTGCGAGTGCGGGCTGGCGGCGCGCGGCAAGGTGGCGTGCACGCAGCCGCGCCGCGTGGCCGCCATGTCCGTCGCCAAGCGCGTTGCCGAGGAGTTCGGCTGCCGGCTGGGCCAGGAGGTGGGCTACACCATACGCTTCGAGGACTGCACCAGCCCGGACACTGTCATCAAATACATGACGGACGGCATGCTGCTCCGCGAGTGCCTCATGGACCTGGACCTGAAGGCGTACTCGGTGATCATGCTGGACGAGGCGCACGAGCGCACCATCCACACCGACGTGCTGTTCGGTCTGCTGAAGCAGGCCGTGCAGAAGCGCCCCGAACTCAAGCTCATCGTCACGTCCGCCACGCTCGACGCCGTCAAGTTCTCGCAGTACTTCTTCGAGGCCCCGATCTTCACCATCCCCGGTCGAACCTTCCCCGTCGAAGTCTTGTACACCAAGGAACCGGAGACGGACTATTTGGACGCCTCACTGATCACGGTCATGCAGATCCACCTGAGGGAGCCCCCGGGGGATATCCTGCTGTTCCTCACTGGCCAAGAAGAGATTGACACGGCGTGTGAGATATTGTACGAGAGAATGAAGTCCTTGGGGCCGGACGTGCCGGAGCTGATAATTCTGCCGGTGTACTCTGCGCTGCCGTCCGAGATGCAGACCCGGATCTTCGAGCCGGCGCCCCCCGGCTCCAGGAAGGTGGTGATCGCCACCAACATCGCGGAGACGTCGCTGACCATCGACGGTATATACTACGTGGTGGACCCTGGCTTCGTTAAACAGAAAGTCTACAACTCGAAGACTGGAATGGATTCGTTGGTTGTAACTCCTATATCGCAGGCGGCGGCGAAGCAGCGCGCCGGGCGGGCGGGCCGCACCGGGCCGGGCAAGTGCTACCGGCTGTACACGGAGCGAGCCTACCGGGACGAGATGCTGCCCACGCCCGTGCCCGAGATACAGCGGACCAACCTCGCCACCACCGTATTACAATTGAAAACAATGGGTATAAACGATTTGCTGCATTTCGACTTCATGGACGCGCCGCCGGTCGAGTCGCTCATCATGGCGCTGGAGCAGCTGCACTCGCTGTCGGCGCTCGACTCTGAGGGACTCCTGACGAGGCTGGGACGCAGAATGGCCGAATTTCCACTAGAGCCAAACCTATCGAAGATCCTAATCATGTCGGTGGCCCTGCAGTGCTCGGATGAAATACTCACAATAGTGTCGATGCTGAGCGTCCAAAACGTTTTCTATCGGCCGAAGGACAAGCAGGCGCTTGCCGACCAGAAGAAGGCCAAGTTCAACCAGCCGGAAGGGGACCACCTGACGCTACTGGCCGTGTACAACAGCTGGAGGAATAACAAGTTCTCGAACGCGTGGTGCTACGAAAACTTCGTGCAGATCAGGACGTTGAAGCGGGCCCAGGACGTGAGGAAGCAGCTCCTCGGGATCATGGACAGACACAAATTAGACGTAGTTTCAGCCGGTAAGAATACAGTTCGCATTCAGAAGACGATATGCTCGGGCTTCTTCAGGAACGCGGCCAAGAAGGATCCCCAGGAAGGATATAGGACGCTCGTGGACAGTCAAGTTGTTTATATACATCCTTCCAGTGCACTGTTCAACAGACAACCGGAATGGGTAATCTACCATGAGCTAGTACAGACTACGAAAGAGTACATGAGGGAAGTCACGACCATAGACCCGAAATGGCTGGTTGAGTTTGCTCCCGCCTTCTTTAAATTCTCGGACCCAACAAAACTCTCTAAATTTAAGAAGAACCAGAGACTGGAACCGTTGTACAATAAATATGAGGAGCCGAACGCTTGGAGGATATCGAGAGTCAGACGCAGAAGAAATTAG

Protein

MDEVTKLEHLSLVSKICTELDNHLGLNDKDLAEFIIDLADKNPTFETFKVALVENGAEFSDSFMTNLLRIIQHMKPVNTSTLIKNIAETESENPLAKKFPGLAIPNDKPQSFSDESSDDEKDKKRITAKDLFTEEIKPSSTDNVVDLAMAELEALAPSNSTTNELLIGKEPLKIESSRNSDHKEKNKSEESRDKESRSRNPKRSRSRERKRSRSTQNTDKYKLEESRDRKRHSRSRDRKRSRSRDRKRSRSRDRRRSRSRDYKRSRSRERKRSRSKGKRSRSRDRRSKSQDRDRRRRSHSRHRHRSRSRDRRRSYSKSRRSVSGGRRSRSRNREEKFDYGKKSRKRSPEVELNDDPEPGKIYSGRVANIVPFGCFVQIEGVRKRWEGLVHISQLRAEGRVTNVSDVVNRGDKVKVKVISITGQKVSLTMKDVCQETGKDLNPASHAHLEAEREGRNPDRPPPAATVLAGLQGGIDPDEDSSRKRVTRISSPERWEIKQMISSGVIDKSELPDFDEETGLLPKDEDGEADIEIELVEEEPPFLQGHGRALHDLSPVRIVKNPDGSLAQAAMMQSALAKERREQKMIQREQEMDSVPTGLNKNWIDPLPESEGRTLAANMRGAGVVPQDLPEWKKHVIGGKKSSFGKKTNLSLLEQRQSLPIYKLRDELTKAVSDNQILIVIGETGSGKTTQITQYVCECGLAARGKVACTQPRRVAAMSVAKRVAEEFGCRLGQEVGYTIRFEDCTSPDTVIKYMTDGMLLRECLMDLDLKAYSVIMLDEAHERTIHTDVLFGLLKQAVQKRPELKLIVTSATLDAVKFSQYFFEAPIFTIPGRTFPVEVLYTKEPETDYLDASLITVMQIHLREPPGDILLFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFEPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGMDSLVVTPISQAAAKQRAGRAGRTGPGKCYRLYTERAYRDEMLPTPVPEIQRTNLATTVLQLKTMGINDLLHFDFMDAPPVESLIMALEQLHSLSALDSEGLLTRLGRRMAEFPLEPNLSKILIMSVALQCSDEILTIVSMLSVQNVFYRPKDKQALADQKKAKFNQPEGDHLTLLAVYNSWRNNKFSNAWCYENFVQIRTLKRAQDVRKQLLGIMDRHKLDVVSAGKNTVRIQKTICSGFFRNAAKKDPQEGYRTLVDSQVVYIHPSSALFNRQPEWVIYHELVQTTKEYMREVTTIDPKWLVEFAPAFFKFSDPTKLSKFKKNQRLEPLYNKYEEPNAWRISRVRRRRN

Description

Involved in pre-mRNA splicing as component of the spliceosome (PubMed:18981222, PubMed:24244416). Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome (By similarity). Before and after egg-chamber formation, required for nurse-cell chromatin dispersal (NCCD) probably by playing a role in spliceosome localization to chromatin/interchromatin spaces (PubMed:24244416).

Catalytic Activity

ATP + H2O = ADP + H(+) + phosphate

Subunit

Identified in the spliceosome C complex.

Similarity

Belongs to the DEAD box helicase family. DEAH subfamily. DDX8/PRP22 sub-subfamily.
Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.

Keywords

ATP-binding   Complete proteome   DNA condensation   Helicase   Hydrolase   mRNA processing   mRNA splicing   Nucleotide-binding   Nucleus   Reference proteome   Spliceosome  

Feature

chain  ATP-dependent RNA helicase DHX8

Uniprot

A0A2H1VKX4   A0A2A4J898   A0A194Q471   A0A1W4XVV8   A0A1Y1JYU8   D6WZK3   + More
A0A1B6EKN4   B3MD00   A0A2J7QXS3   A0A067QSK0   B4P6M1   B3NRD1   B4KT85   A0A0L0BUI9   A1Z9L3   A0A1W4VCF7   B4LNR3   B4HR09   A0A1I8PWI5   B4MY71   A0A0M9A3B0   A0A1B0FP40   Q292F4   B4JVH2   A0A2A3E6G1   A0A1J1J6C9   A0A1I8MJW9   A0A1B0BUZ3   A0A0M5IY18   A0A182GUX7   A0A088ALV6   B4GDM0   A0A1A9WBZ7   A0A1A9Y2J5   A0A0L7QSX8   A0A154PBE0   A0A1A9VJ30   Q16PT8   A0A182M4X4   A0A0J7NWU6   A0A195C6Y5   A0A182R3J2   A0A3B0J4F9   A0A182YML3   E2AVZ5   A0A182WJR8   A0A151X4R7   A0A026X145   E9IGS5   A0A1Q3F680   E0VM14   A0A158NDX0   A0A084WER8   F4W7U5   A0A195BPP4   A0A182JYP7   A0A182N920   W8C3S0   A0A182PP37   A0A182XLJ4   A0A182V082   E2BY63   A0A182KQN5   A0A182JEE3   A0A182UC60   A0A195D7W1   A0A182I2Q9   A0A182QLE3   A0A195ERA4   Q7PT33   W5J8A4   A0A224XI80   A0A023F5M0   B0WVX3   A0A0K8WDI6   A0A034VE32   A0A2M4A9C4   A0A2M4B9Q7   A0A182FGC7   A0A0A9YMY4   A0A336M1Z5   A0A1B0CYN0   A0A0J9RCJ8   A0A1A9Z7A1   K7J1G4   A0A310SB34   A0A2S2R601   J9K579   T1IZH9   A0A232FI38   T1HWH8   A0A131YM49   A0A224YNR5   A0A131Y0N9   A0A2R5LI29   A0A131XCM6   A0A0P5ZPF8   A0A0P5VN56  

EC Number

3.6.4.13

Pubmed

26354079    28004739    18362917    19820115    17994087    24845553    + More
17550304    26108605    10731132    12537572    12537569    18981222    24244416    15632085    25315136    26483478    17510324    25244985    20798317    24508170    30249741    21282665    20566863    21347285    24438588    21719571    24495485    20966253    12364791    14747013    17210077    20920257    23761445    25474469    25348373    25401762    26823975    22936249    20075255    28648823    26830274    28797301    29652888    28049606   

EMBL

ODYU01003117    SOQ41467.1    NWSH01002488    PCG68191.1    KQ459472    KPJ00144.1    + More
GEZM01103018    GEZM01103017    GEZM01103016    GEZM01103015    JAV51617.1    KQ971372    EFA09691.1    GECZ01031275    JAS38494.1    CH902619    EDV36315.1    NEVH01009372    PNF33382.1    KK853074    KDR11769.1    CM000158    EDW90973.1    CH954179    EDV56083.1    CH933808    EDW10597.2    JRES01001317    KNC23658.1    AE013599    AY118656    BT044222    AAM50025.1    ACH92287.1    CH940648    EDW60133.1    CH480816    EDW47806.1    CH963894    EDW77060.2    KQ435760    KOX75586.1    CCAG010023115    CM000071    EAL24908.1    CH916375    EDV98440.1    KZ288353    PBC27300.1    CVRI01000069    CRL07030.1    JXJN01020988    CP012524    ALC42456.1    JXUM01018693    JXUM01018694    KQ560519    KXJ82015.1    CH479181    EDW31677.1    KQ414756    KOC61659.1    KQ434868    KZC09161.1    CH477773    EAT36372.1    AXCM01002191    LBMM01001117    KMQ96895.1    KQ978251    KYM95936.1    OUUW01000001    SPP74383.1    GL443213    EFN62417.1    KQ982548    KYQ55280.1    KK107046    QOIP01000004    EZA61721.1    RLU23672.1    GL763054    EFZ20200.1    GFDL01011974    JAV23071.1    DS235286    EEB14420.1    ADTU01013059    ATLV01023246    KE525341    KFB48712.1    GL887888    EGI69615.1    KQ976424    KYM88480.1    GAMC01002647    JAC03909.1    GL451420    EFN79342.1    KQ981153    KYN08975.1    APCN01000178    AXCN02000540    KQ981993    KYN30790.1    AAAB01008807    EAA04624.5    ADMH02002070    ETN59623.1    GFTR01008583    JAW07843.1    GBBI01002253    JAC16459.1    DS232134    EDS35805.1    GDHF01003404    JAI48910.1    GAKP01019149    JAC39803.1    GGFK01004066    MBW37387.1    GGFJ01000601    MBW49742.1    GBHO01010066    GBHO01010065    GBRD01005381    GDHC01020627    GDHC01004880    JAG33538.1    JAG33539.1    JAG60440.1    JAP98001.1    JAQ13749.1    UFQT01000289    SSX22853.1    AJVK01009248    CM002911    KMY93686.1    KQ773953    OAD52333.1    GGMS01016150    MBY85353.1    ABLF02023620    AFFK01020457    NNAY01000193    OXU30118.1    ACPB03016233    GEDV01008935    JAP79622.1    GFPF01006243    MAA17389.1    GEFM01003761    GEGO01006586    JAP72035.1    JAR88818.1    GGLE01005030    MBY09156.1    GEFH01004731    JAP63850.1    GDIP01044533    JAM59182.1    GDIP01097776    JAM05939.1   

Proteomes

UP000218220    UP000053268    UP000192223    UP000007266    UP000007801    UP000235965    + More
UP000027135    UP000002282    UP000008711    UP000009192    UP000037069    UP000000803    UP000192221    UP000008792    UP000001292    UP000095300    UP000007798    UP000053105    UP000092444    UP000001819    UP000001070    UP000242457    UP000183832    UP000095301    UP000092460    UP000092553    UP000069940    UP000249989    UP000005203    UP000008744    UP000091820    UP000092443    UP000053825    UP000076502    UP000078200    UP000008820    UP000075883    UP000036403    UP000078542    UP000075900    UP000268350    UP000076408    UP000000311    UP000075920    UP000075809    UP000053097    UP000279307    UP000009046    UP000005205    UP000030765    UP000007755    UP000078540    UP000075881    UP000075884    UP000075885    UP000076407    UP000075903    UP000008237    UP000075882    UP000075880    UP000075902    UP000078492    UP000075840    UP000075886    UP000078541    UP000007062    UP000000673    UP000002320    UP000069272    UP000092462    UP000092445    UP000002358    UP000007819    UP000215335    UP000015103   

PRIDE

A1Z9L3   

Pfam

PF00270   DEAD        + More
PF12483   GIDE
PF07717   OB_NTP_bind
PF00271   Helicase_C
PF04408   HA2
PF00575   S1
PF03097   BRO1
PF13949   ALIX_LYPXL_bnd
PF00398   RrnaAD

Interpro

IPR013083   Znf_RING/FYVE/PHD        + More
IPR011545   DEAD/DEAH_box_helicase_dom       
IPR012340   NA-bd_OB-fold       
IPR011709   DUF1605       
IPR001650   Helicase_C       
IPR014001   Helicase_ATP-bd       
IPR007502   Helicase-assoc_dom       
IPR001841   Znf_RING       
IPR003029   S1_domain       
IPR027417   P-loop_NTPase       
IPR002464   DNA/RNA_helicase_DEAH_CS       
IPR022967   S1_dom       
IPR022170   GIDE       
IPR042035   DEAH_win-hel_dom       
IPR004328   BRO1_dom       
IPR038499   BRO1_sf       
IPR025304   ALIX_V_dom       
IPR001737   KsgA/Erm       
IPR029063   SAM-dependent_MTases       

SUPFAM

SSF52540   SSF52540        + More
SSF50249   SSF50249       
SSF53335   SSF53335       

Gene 3D

3.30.40.10     1.10.10.2130     1.25.40.280    

CDD

cd00079  HELICc

ProteinModelPortal

A0A2H1VKX4   A0A2A4J898   A0A194Q471   A0A1W4XVV8   A0A1Y1JYU8   D6WZK3   + More
A0A1B6EKN4   B3MD00   A0A2J7QXS3   A0A067QSK0   B4P6M1   B3NRD1   B4KT85   A0A0L0BUI9   A1Z9L3   A0A1W4VCF7   B4LNR3   B4HR09   A0A1I8PWI5   B4MY71   A0A0M9A3B0   A0A1B0FP40   Q292F4   B4JVH2   A0A2A3E6G1   A0A1J1J6C9   A0A1I8MJW9   A0A1B0BUZ3   A0A0M5IY18   A0A182GUX7   A0A088ALV6   B4GDM0   A0A1A9WBZ7   A0A1A9Y2J5   A0A0L7QSX8   A0A154PBE0   A0A1A9VJ30   Q16PT8   A0A182M4X4   A0A0J7NWU6   A0A195C6Y5   A0A182R3J2   A0A3B0J4F9   A0A182YML3   E2AVZ5   A0A182WJR8   A0A151X4R7   A0A026X145   E9IGS5   A0A1Q3F680   E0VM14   A0A158NDX0   A0A084WER8   F4W7U5   A0A195BPP4   A0A182JYP7   A0A182N920   W8C3S0   A0A182PP37   A0A182XLJ4   A0A182V082   E2BY63   A0A182KQN5   A0A182JEE3   A0A182UC60   A0A195D7W1   A0A182I2Q9   A0A182QLE3   A0A195ERA4   Q7PT33   W5J8A4   A0A224XI80   A0A023F5M0   B0WVX3   A0A0K8WDI6   A0A034VE32   A0A2M4A9C4   A0A2M4B9Q7   A0A182FGC7   A0A0A9YMY4   A0A336M1Z5   A0A1B0CYN0   A0A0J9RCJ8   A0A1A9Z7A1   K7J1G4   A0A310SB34   A0A2S2R601   J9K579   T1IZH9   A0A232FI38   T1HWH8   A0A131YM49   A0A224YNR5   A0A131Y0N9   A0A2R5LI29   A0A131XCM6   A0A0P5ZPF8   A0A0P5VN56  

PDB

6QDV     E-value=0,     Score=3769

KEGG

101739903    K12818 ATP-dependent RNA helicase DHX8/PRP22 [EC:3.6.4.13] | (RefSeq) ATP-dependent RNA helicase DHX

PATHWAY

03040    Spliceosome - Bombyx mori (domestic silkworm)

GO

GO:0005524   GO:0006996   GO:0004386   GO:0003676   GO:0004842   GO:0016021   GO:0003723   GO:0071013   GO:0000390   GO:0034459   GO:0006325   GO:0016887   GO:0048477   GO:0005681   GO:0005634   GO:0000398   GO:0071011   GO:0000381   GO:0030261   GO:0007419   GO:0004004   GO:0000179   GO:0008026   GO:0008270   GO:0006508   GO:0005622   GO:0007264   GO:0016597  

Subcellular location

Nucleus  

Length:

1314

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.10795

Exp number, first 60 AAs:

0

Total prob of N-in:

0.00001

outside

1  -  1314

 

 

Pi

15.898324

Theta

15.58283

Tajima's D

-1.891226

CLR

331.544244

CSRT

0.0196990150492475

Interpretation

Possibly Positive selection