Gene

KWMTBOMO05972  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA002896

Annotation

PREDICTED:_mitochondrial_ubiquitin_ligase_activator_of_nfkb_1-A_[Papilio_xuthus]

Location

Bomo_Chr10(-):15644699-15653217      View in EpiBrowser

Full name

Mitochondrial E3 ubiquitin protein ligase 1      

Alternative Name

Mitochondrial E3 ubiquitin protein transferase 1

Location in the cell

Nuclear   Reliability : 1.424 PlasmaMembrane   Reliability : 1.538

CDS

ATGGATTTTTTTTCGGAAATTATAGGCGAGACGGTCATTTTAGGGTTAGATTCATTAATATTGGGATTCTGCGTGAAGCAGCTGAGCAAATGTAAACACATCCTCAATGCTTTACAGACAGCACCAGTTCTGGAGATAGACTCATCAATTAACAAAGAATTAAAGAAGTTCCCCAATAGCACAATCCCATATGTCGTGATAAGAGGACTGGTGAAGCCGCTTGGCACCCCTATCACCAGTAATTACAATAACTCCATCACCGGTGTGGTGCAAAGATTAACAATAAAAGAGCATGTAATAGCCAGAACATCGGCAGGCTTCTGGTCAGACCAAACCCGGACCATTCATGAAGTGTGTAATTCGACTCCATTTGTTCTCAACAATGGGAAATACAGCATAGAAGTTGTTGACGCACTAGCTGCTGAATTACTTGATATGGACGTGATTTCGGATAAATTCGAACCGATGTCTCCTGGTGTTATCGATCACGTATGGGGCTTCTTCTCCGGAGTGCGTCAGCGAGGACTCCAGACTATGGAGGAGATGCTTCGAGACGGCTCCTACATTACTGCCATCGGCGAACTGAGCAAGAGCAAAACAGGCGCACTAAAGATCCAGCCTCCCAGAGACGGCCTACCGCTGTACTTGACCACAGCCACGAAATCCAGTTTGTTGAAGAGGTTGGCCAGTTCGAGAGATTTTTTGAGAATCCTGGTGGTAATATTCGGTGCGGTGGCCGTGGCGGCCTCGTGCAGGGTCGCTTACAAGTACGTGAAGCGACGCAAGCGACGCGCGCTCGACGACTCAGTGAAGCAGCGGCTGGCGGCCGGGCGGCGGGAGAGACGAGCCGTGGCCAGAGAGAAGAACCTGAGCGAAGTGCAACTCTGCGTTGTCTGTACAGAGAATCCTAAGGAGATTATACTCTTACCGTGCGGTCACGTGTGTGTGTGCGAGGATTGCTCGGACAACATCACGGACAACTGCCCCATATGCCGTAAAAGAATAGAATCCAGGGCGCCCGCCTTCATAACATAA

Protein

MDFFSEIIGETVILGLDSLILGFCVKQLSKCKHILNALQTAPVLEIDSSINKELKKFPNSTIPYVVIRGLVKPLGTPITSNYNNSITGVVQRLTIKEHVIARTSAGFWSDQTRTIHEVCNSTPFVLNNGKYSIEVVDALAAELLDMDVISDKFEPMSPGVIDHVWGFFSGVRQRGLQTMEEMLRDGSYITAIGELSKSKTGALKIQPPRDGLPLYLTTATKSSLLKRLASSRDFLRILVVIFGAVAVAASCRVAYKYVKRRKRRALDDSVKQRLAAGRRERRAVAREKNLSEVQLCVVCTENPKEIILLPCGHVCVCEDCSDNITDNCPICRKRIESRAPAFIT

Description

Exhibits weak E3 ubiquitin-protein ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. Plays a role in the control of mitochondrial morphology by promoting mitochondrial fission. Negatively regulates the mitochondrial fusion protein marf by promoting its ubiquitination, acting in a pathway that is parallel to the park/pink1 regulatory pathway.

Catalytic Activity

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.

Subunit

Interacts with Marf.

Keywords

Complete proteome   Isopeptide bond   Membrane   Metal-binding   Mitochondrion   Mitochondrion outer membrane   Reference proteome   Transferase   Transmembrane   Transmembrane helix   Ubl conjugation   Ubl conjugation pathway   Zinc   Zinc-finger  

Feature

chain  Mitochondrial E3 ubiquitin protein ligase 1

Uniprot

H9J061   A0A194Q3M7   A0A3S2P1W0   A0A194QRL0   A0A212EIU6   A0A2H1VKX4   + More
A0A0L7K5C2   A0A154PJB2   A0A2A3EKE4   A0A088APU7   A0A0M8ZSF5   A0A232EN46   K7JC86   A0A1W4X9W2   A0A0L7QM62   A0A195B6R3   A0A158NGV7   A0A0T6B6Y5   A0A0J7L5H1   D6X2I9   A0A195DXY7   A0A151IIC8   A0A195FFN0   A0A1Y1K2J0   F4WZS8   E2AM11   A0A2M3ZGX1   A0A151XD20   A0A2M4BU25   Q0IF35   A0A182FH01   A0A2M3ZGP2   A0A182X716   A0A182V0G1   A0A182UCQ1   A0A182KPL9   Q7Q6A1   A0A182I1W4   W5JP86   A0A2M3Z3W6   A0A182MC16   A0A2M4ACU0   A0A026X286   E2BD82   A0A182J2Y4   Q16RU2   A0A1I8MEK4   A0A182Q790   A0A1A9YEK7   A0A1B0B7T3   A0A1I8PW47   A0A182NIM5   A0A182WKP8   A0A0M4EMW8   E9J897   A0A182JUD0   A0A0L0CKX3   A0A182YD15   A0A1W4VSC9   A0A034VK99   B3M8E2   B0WPA5   D3TMJ3   A0A1A9VN06   A0A067QY73   A0A182PV31   A0A1Q3FAG0   A0A0K8V853   A0A1B0A2J7   W8BEV7   A0A1A9X0D7   A0A084WAA8   B4N4X3   B4KV23   A0A182RZP2   Q9VZJ9   B4HTX6   J3JV07   B4LBG1   B4J0G9   B3NG13   B4PH94   B4QQ20   A0A3B0JXS0   A0A182SL57   N6U3Y9   R4G8R1   A0A0K8TS00   A0A0Q9X6D5   A0A069DSX6   A0A0P4VUK6   E0W0K4   B4H5P4   Q29EY5   A0A0A9W311   A0A2P8YKA0   B4GQ16   B5DIZ4   A0A1S4EB29  

EC Number

2.3.2.27

Pubmed

19121390    26354079    22118469    26227816    28648823    20075255    + More
21347285    18362917    19820115    28004739    21719571    20798317    17510324    20966253    12364791    14747013    17210077    20920257    23761445    24508170    30249741    25315136    21282665    26108605    25244985    25348373    17994087    20353571    24845553    24495485    24438588    10731132    12537572    12537569    24898855    22516182    17550304    22936249    23537049    26369729    26334808    27129103    20566863    15632085    25401762    26823975    29403074   

EMBL

BABH01018980    KQ459472    KPJ00143.1    RSAL01000002    RVE54980.1    KQ461175    + More
KPJ07969.1    AGBW02014561    OWR41416.1    ODYU01003117    SOQ41467.1    JTDY01010735    KOB58058.1    KQ434936    KZC11935.1    KZ288232    PBC31501.1    KQ435922    KOX68673.1    NNAY01003224    OXU19770.1    KQ414905    KOC59611.1    KQ976574    KYM80186.1    ADTU01015279    ADTU01015280    LJIG01009414    KRT83128.1    LBMM01000653    KMQ97871.1    KQ971372    EFA09449.1    KQ980107    KYN17738.1    KQ977492    KYN02310.1    KQ981625    KYN39161.1    GEZM01098608    JAV53835.1    GL888480    EGI60353.1    GL440703    EFN65531.1    GGFM01007008    MBW27759.1    KQ982294    KYQ58257.1    GGFJ01007117    MBW56258.1    CH477420    EAT41238.1    GGFM01006986    MBW27737.1    AAAB01008960    EAA11719.1    APCN01000099    ADMH02000762    ETN65133.1    GGFM01002461    MBW23212.1    AXCM01000852    GGFK01005283    MBW38604.1    KK107020    QOIP01000004    EZA62430.1    RLU23551.1    GL447585    EFN86310.1    CH477696    EAT37142.1    AXCN02000417    JXJN01009712    CP012525    ALC43167.1    GL768914    EFZ10953.1    JRES01000254    KNC32925.1    GAKP01016742    JAC42210.1    CH902618    EDV38877.1    DS232021    EDS32155.1    CCAG010017532    EZ422645    ADD18921.1    KK852824    KDR15431.1    GFDL01010536    JAV24509.1    GDHF01017190    JAI35124.1    GAMC01011092    JAB95463.1    ATLV01022061    KE525327    KFB47152.1    CH964101    EDW79412.1    CH933809    EDW18334.1    AE014296    AY113263    AAM29268.1    CH480817    EDW50397.1    BT127072    AEE62034.1    CH940647    EDW69749.1    CH916366    EDV96805.1    CH954178    EDV50842.1    CM000159    EDW93331.1    CM000363    CM002912    EDX09140.1    KMY97464.1    OUUW01000009    SPP85222.1    APGK01050248    KB741169    KB632294    ENN73297.1    ERL91656.1    ACPB03002190    GAHY01000532    JAA76978.1    GDAI01000476    JAI17127.1    CH964251    KRF99751.1    GBGD01002068    JAC86821.1    GDKW01000885    JAI55710.1    DS235860    EEB19160.1    CH479211    EDW33096.1    CH379070    EAL29924.1    GBHO01041420    GBRD01014595    GDHC01010702    JAG02184.1    JAG51231.1    JAQ07927.1    PYGN01000534    PSN44666.1    CH479187    EDW39688.1    CH379060    EDY70288.1   

Proteomes

UP000005204    UP000053268    UP000283053    UP000053240    UP000007151    UP000037510    + More
UP000076502    UP000242457    UP000005203    UP000053105    UP000215335    UP000002358    UP000192223    UP000053825    UP000078540    UP000005205    UP000036403    UP000007266    UP000078492    UP000078542    UP000078541    UP000007755    UP000000311    UP000075809    UP000008820    UP000069272    UP000076407    UP000075903    UP000075902    UP000075882    UP000007062    UP000075840    UP000000673    UP000075883    UP000053097    UP000279307    UP000008237    UP000075880    UP000095301    UP000075886    UP000092443    UP000092460    UP000095300    UP000075884    UP000075920    UP000092553    UP000075881    UP000037069    UP000076408    UP000192221    UP000007801    UP000002320    UP000092444    UP000078200    UP000027135    UP000075885    UP000092445    UP000091820    UP000030765    UP000007798    UP000009192    UP000075900    UP000000803    UP000001292    UP000008792    UP000001070    UP000008711    UP000002282    UP000000304    UP000268350    UP000075901    UP000019118    UP000030742    UP000015103    UP000009046    UP000008744    UP000001819    UP000245037    UP000079169   

PRIDE

H9J061    Q9VZJ9   

Pfam

PF12483   GIDE        + More
PF00270   DEAD
PF07717   OB_NTP_bind
PF00271   Helicase_C
PF04408   HA2
PF00575   S1

Interpro

IPR001841   Znf_RING        + More
IPR022170   GIDE       
IPR013083   Znf_RING/FYVE/PHD       
IPR011545   DEAD/DEAH_box_helicase_dom       
IPR012340   NA-bd_OB-fold       
IPR011709   DUF1605       
IPR001650   Helicase_C       
IPR014001   Helicase_ATP-bd       
IPR007502   Helicase-assoc_dom       
IPR003029   S1_domain       
IPR027417   P-loop_NTPase       
IPR002464   DNA/RNA_helicase_DEAH_CS       
IPR022967   S1_dom       

SUPFAM

SSF52540   SSF52540        + More
SSF50249   SSF50249       

Gene 3D

3.30.40.10    

CDD

cd00079  HELICc

ProteinModelPortal

H9J061   A0A194Q3M7   A0A3S2P1W0   A0A194QRL0   A0A212EIU6   A0A2H1VKX4   + More
A0A0L7K5C2   A0A154PJB2   A0A2A3EKE4   A0A088APU7   A0A0M8ZSF5   A0A232EN46   K7JC86   A0A1W4X9W2   A0A0L7QM62   A0A195B6R3   A0A158NGV7   A0A0T6B6Y5   A0A0J7L5H1   D6X2I9   A0A195DXY7   A0A151IIC8   A0A195FFN0   A0A1Y1K2J0   F4WZS8   E2AM11   A0A2M3ZGX1   A0A151XD20   A0A2M4BU25   Q0IF35   A0A182FH01   A0A2M3ZGP2   A0A182X716   A0A182V0G1   A0A182UCQ1   A0A182KPL9   Q7Q6A1   A0A182I1W4   W5JP86   A0A2M3Z3W6   A0A182MC16   A0A2M4ACU0   A0A026X286   E2BD82   A0A182J2Y4   Q16RU2   A0A1I8MEK4   A0A182Q790   A0A1A9YEK7   A0A1B0B7T3   A0A1I8PW47   A0A182NIM5   A0A182WKP8   A0A0M4EMW8   E9J897   A0A182JUD0   A0A0L0CKX3   A0A182YD15   A0A1W4VSC9   A0A034VK99   B3M8E2   B0WPA5   D3TMJ3   A0A1A9VN06   A0A067QY73   A0A182PV31   A0A1Q3FAG0   A0A0K8V853   A0A1B0A2J7   W8BEV7   A0A1A9X0D7   A0A084WAA8   B4N4X3   B4KV23   A0A182RZP2   Q9VZJ9   B4HTX6   J3JV07   B4LBG1   B4J0G9   B3NG13   B4PH94   B4QQ20   A0A3B0JXS0   A0A182SL57   N6U3Y9   R4G8R1   A0A0K8TS00   A0A0Q9X6D5   A0A069DSX6   A0A0P4VUK6   E0W0K4   B4H5P4   Q29EY5   A0A0A9W311   A0A2P8YKA0   B4GQ16   B5DIZ4   A0A1S4EB29  

PDB

3T6P     E-value=4.05321e-06,     Score=120

KEGG

101739767    K15688 E3 ubiquitin-protein ligase MUL1 [EC:2.3.2.27] | (RefSeq) mitochondrial ubiquitin ligase acti

GO

GO:0006996   GO:0016021   GO:0004842   GO:0016874   GO:0005524   GO:0004386   GO:0003676   GO:0016567   GO:0090141   GO:0008270   GO:0005777   GO:0031307   GO:0005515   GO:0006508   GO:0005622   GO:0007264   GO:0008026   GO:0016597  

Subcellular location

Mitochondrion outer membrane  

Length:

344

Number of predicted TMHs:

1

Exp number of AAs in TMHs:

21.5304

Exp number, first 60 AAs:

0.23841

Total prob of N-in:

0.01084

outside

1  -  232

TMhelix

233  -  255

inside

256  -  344

 

 

Pi

24.029319

Theta

2.978718

Tajima's D

-1.572641

CLR

20.125378

CSRT

0.0414979251037448

Interpretation

Possibly Positive selection