Gene

KWMTBOMO05860

Pre Gene Modal

BGIBMGA006968

Annotation

kynurenine_3-monooxygenase_[Bombyx_mori]

Location

Bomo_Chr10(+):12645226-12660348      View in EpiBrowser

Full name

Kynurenine 3-monooxygenase      

Alternative Name

Kynurenine 3-hydroxylase

Location in the cell

Mitochondrial   Reliability : 1.161 PlasmaMembrane   Reliability : 1.722

CDS

ATGGCGAATTTAGAGAATGGAACGTTCAAGAAACTAGATGTGGTTGTTGTCGGTGGAGGTTTGGTGGGATCACTCGAAGCATTATTTTTGGCGAAGAGAGGACATCGCGTTCGCCTGTACGAATATCGAGAAGACATAAGAAATACTCCACAGGCCCGAGGTAGATCCATAAACCTGGCTTTGTCTATACGAGGCAGAACGGCGTTGAGAGAAGTCGGTTTAGAAGACCACATGATCAATAATCATGGGATCCCCATGAAAGGACGCAACATCCACCGAATCGATGGTTCCACTTATATTATTCCTTATGATTCTAGAACTAAACAATGTATTTATTCCGTTGGACGAAATTATTTAAATGGACTATTATTACAAGAATCTGAAAAGTATGAAAACGTGGAGAGATTTTTTAATCACAAACTGATAGCATCAAATTTGAGAAAAGGTTTTTTATCTTTTCAGAAGACTGACACCAAAGAAATAGTTGAAGTCAATGCAGACCTAATCATAGGGGCCGACGGTGCATTTTCAGCTGTGCGCAAAGAAATTATGAAACAACCACTTTTTGACTACAATCAGAAATATATTGAACATGGATATTTGGAATTATGCATACCCCCTGATAGCAATGGCGGATTTCAGATGCCACCGAACTATCTGCATATCTGGCCTAGGGGTGAATTTATGATGATCGCTCTTCCAAACCAGGACTGCTCCTGGACTGTGACACTTTTCATGCCATTCACACACTTCAAAAGTTTAGATAACGAAGATAAATTATTGAAGTTTTTCGAAAAATATTTCCCAGATTCAATTCCGCTGATAGGAAAACAAAAGTTAATAGCCGACTATTTTGCCGGATCAGCTTCGCCTTTGATTGCGATTAAGTGTCGACCTTACAATGTAGAAGACAAAGCTCTGATCATTGGTGACGCAGCTCACGCTGTGGTTCCATTTTACGGCCAAGGTATGAACGCTGGCTTCGAAGATTGCACGATCCTCAATCAATTATTCCAGAAGCACCATGATGATCTGGCTAAAATACTGAAAGAATTCTCTGACACCAGATGGGAGGATACTTTTGCAATTAGTGATCTGGCTATGTACAATTATATTGAGATGCGAGATCTTGTAACACGACCATCCTATCTCCTGCGAAAAGCTATTGATGACGTCATTTTTTGGTTGATGCCCAAAGTTTGGATTCCTCTCTACAACTCTGTGACATTCTCCACGATCCCATACACTCAATGTATCAAAAATCGTCAATGGCAGGATAAGGTTTTAAATCGGTCACTTTTGTTCCTTGGTACCGTCACGAGTGCTGTAGGAGCGTTCTACGTTTATAAAAAATTTGTAGGCTTAAGCTAA

Protein

MANLENGTFKKLDVVVVGGGLVGSLEALFLAKRGHRVRLYEYREDIRNTPQARGRSINLALSIRGRTALREVGLEDHMINNHGIPMKGRNIHRIDGSTYIIPYDSRTKQCIYSVGRNYLNGLLLQESEKYENVERFFNHKLIASNLRKGFLSFQKTDTKEIVEVNADLIIGADGAFSAVRKEIMKQPLFDYNQKYIEHGYLELCIPPDSNGGFQMPPNYLHIWPRGEFMMIALPNQDCSWTVTLFMPFTHFKSLDNEDKLLKFFEKYFPDSIPLIGKQKLIADYFAGSASPLIAIKCRPYNVEDKALIIGDAAHAVVPFYGQGMNAGFEDCTILNQLFQKHHDDLAKILKEFSDTRWEDTFAISDLAMYNYIEMRDLVTRPSYLLRKAIDDVIFWLMPKVWIPLYNSVTFSTIPYTQCIKNRQWQDKVLNRSLLFLGTVTSAVGAFYVYKKFVGLS

Description

Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid.

Catalytic Activity

H(+) + L-kynurenine + NADPH + O2 = 3-hydroxy-L-kynurenine + H2O + NADP(+)

Cofactor

FAD

Biophysicochemical Properties

0.89 mM for L-kynurenine
0.82 mM for NADPH

Similarity

Belongs to the aromatic-ring hydroxylase family. KMO subfamily.

Keywords

Complete proteome   FAD   Flavoprotein   Membrane   Mitochondrion   Monooxygenase   NADP   Oxidoreductase   Pyridine nucleotide biosynthesis   Reference proteome   Transmembrane   Transmembrane helix   Alternative splicing  

Feature

chain  Kynurenine 3-monooxygenase
splice variant  In isoform 2.

Uniprot

Q95NK3   A2ID62   Q1PBJ7   B0FXJ7   A2ID61   A2ID64   + More
A2ID63   A2ID66   Q966T2   Q966T1   A0A3S2LIV6   A0A2H1WDP9   O76150   A0A194QQV5   A0A194Q856   A0A0N0PAS9   A0A182JY04   A0A182PH63   A0A084WAA1   Q7Q6A7   A0A182RZP9   A0A182TR92   A0A182UTE1   A0A182QIT8   A0A182SFD0   Q86PM2   A0A182I1X5   T1DNM2   A0A182NIN3   A0A182GB70   A0A2K7P6B9   A0A182IX05   A0A182YD21   A0A182WKQ4   W5JA14   Q8ISJ5   A0A182FH06   B0XCN2   A0A182MTN1   A0A182TR63   A0A182GP36   A0A182X709   A0A1Q3FQY7   A0A182KPN6   U5EKF9   A0A1J1IT62   A0A1B0DR24   A0A1B6LYM2   A0A212EIH6   A0A1L8DZZ5   A0A0P5K040   A0A0P5ZMC5   A0A190EF52   D6W8X0   W8AKN7   A0A336K959   A0A164XEN7   A0A1B6D6B2   A0A0A1X5Y3   A0A1Z5KUA2   A0A232ES08   A0A0A1XJW2   A0A034W1L3   A0A034W022   A0A2R5L6J7   E9H0N5   Q95NP6   A0A1L8DZW6   A0A0K8VXF8   A0A0K8W6G4   A0A023GK71   B1B6U6   A0A1E1XET2   K7IYV7   P0CU30   A0A0P5ED04   A0A1B6F389   A0A293LEA3   A0A2P2I3X1   A0A1B0EXM3   A0A0P4YVR0   A0A1I8MLQ5   A0A1I8PJE6   A0A0P6H6W1   E0VU04   A0A131YYP6   A0A0L0CJS0   Q86PM2-2   A0A224Z2L3   A0A0L0C2Z9   A0A224YU54   B4J4Y6   A0A3B0JNY3   B5DZB4   A0A1A9WJY5   A0A132AFB7   B4HR71   V5HHY6  

EC Number

1.14.13.9

Pubmed

26354079    24438588    12364791    12974953    17510324    26483478    + More
25244985    20920257    23761445    12230548    20966253    22118469    18362917    19820115    24495485    25830018    28528879    28648823    25348373    21292972    11805058    28503490    20075255    17994087    25315136    20566863    26830274    26108605    28797301    15632085    26555130    25765539   

EMBL

EF210331    AB063487    AB063490    ABM68369.1    BAB62416.1    BAB62419.1    + More
EF210328    ABM68366.1    DQ455073    ABE68382.1    EU360772    ABY73874.1    EF210327    ABM68365.1    EF210330    ABM68368.1    EF210329    ABM68367.1    EF210332    ABM68370.1    AB063488    BAB62417.1    AB063489    BAB62418.1    RSAL01000003    RVE54807.1    ODYU01007906    SOQ51096.1    AB011412    BAA31513.1    KQ461175    KPJ07888.1    KQ459302    KPJ01722.1    KQ458479    KPJ05883.1    ATLV01022056    KE525327    KFB47145.1    AAAB01008960    AXCN02000417    AF325508    AY194224    AY194225    CH477539    APCN01000100    GAMD01002715    JAA98875.1    JXUM01052192    JXUM01052193    JXUM01052194    KQ561724    KXJ77718.1    ADMH02001858    ETN60831.1    AY065662    AAL40890.1    DS232707    EDS44928.1    AXCM01009619    JXUM01014806    JXUM01014807    KQ560413    KXJ82544.1    GFDL01005127    JAV29918.1    GANO01001828    JAB58043.1    CVRI01000059    CRL03399.1    AJVK01019570    GEBQ01011198    JAT28779.1    AGBW02014654    OWR41270.1    GFDF01002107    JAV11977.1    GDIQ01191712    JAK60013.1    GDIP01055168    JAM48547.1    KP881329    ALQ52680.1    KQ971312    EEZ98408.1    GAMC01020158    JAB86397.1    UFQS01000187    UFQT01000187    SSX00957.1    SSX21337.1    LRGB01001005    KZS14147.1    GEDC01016076    JAS21222.1    GBXI01007573    JAD06719.1    GFJQ02008301    JAV98668.1    NNAY01002528    OXU21134.1    GBXI01003499    JAD10793.1    GAKP01010927    JAC48025.1    GAKP01010928    JAC48024.1    GGLE01000954    MBY05080.1    GL732581    EFX74720.1    AY052391    AY052393    AAL15465.1    AAL15467.1    GFDF01002113    JAV11971.1    GDHF01008743    JAI43571.1    GDHF01005839    JAI46475.1    GBBM01001094    JAC34324.1    AB428570    BAG12984.1    GFAC01001414    JAT97774.1    CH963849    GDIP01144844    JAJ78558.1    GECZ01025037    JAS44732.1    GFWV01002332    MAA27062.1    IACF01003100    LAB68729.1    AJWK01034186    GDIP01222942    JAJ00460.1    GDIQ01023367    JAN71370.1    DS235777    EEB16860.1    GEDV01004440    JAP84117.1    JRES01000388    KNC31729.1    GFPF01009308    MAA20454.1    JRES01000975    KNC26617.1    GFPF01009309    MAA20455.1    CH916367    EDW01692.1    OUUW01000001    SPP74351.1    CM000071    EDY68800.2    JXLN01013914    KPM09688.1    CH480816    EDW46814.1    GANP01011370    JAB73098.1   

Proteomes

UP000283053    UP000053240    UP000053268    UP000075881    UP000075885    UP000030765    + More
UP000007062    UP000075900    UP000075902    UP000075903    UP000075886    UP000075901    UP000008820    UP000075840    UP000075884    UP000069940    UP000249989    UP000075880    UP000076408    UP000075920    UP000000673    UP000069272    UP000002320    UP000075883    UP000076407    UP000075882    UP000183832    UP000092462    UP000007151    UP000007266    UP000076858    UP000215335    UP000000305    UP000002358    UP000007798    UP000092461    UP000095301    UP000095300    UP000009046    UP000037069    UP000001070    UP000268350    UP000001819    UP000091820    UP000001292   

PRIDE

Q95NK3    A2ID62    Q1PBJ7    B0FXJ7    A2ID61    A2ID64    + More
A2ID63    A2ID66    Q966T2    Q966T1    O76150   

Pfam

PF01494   FAD_binding_3        + More
PF00085   Thioredoxin

Interpro

IPR002938   FAD-bd        + More
IPR036188   FAD/NAD-bd_sf       
IPR027545   Kynurenine_monooxygenase       
IPR036249   Thioredoxin-like_sf       
IPR013766   Thioredoxin_domain       

SUPFAM

SSF51905   SSF51905        + More
SSF52833   SSF52833       

Gene 3D

3.50.50.60    

ProteinModelPortal

Q95NK3   A2ID62   Q1PBJ7   B0FXJ7   A2ID61   A2ID64   + More
A2ID63   A2ID66   Q966T2   Q966T1   A0A3S2LIV6   A0A2H1WDP9   O76150   A0A194QQV5   A0A194Q856   A0A0N0PAS9   A0A182JY04   A0A182PH63   A0A084WAA1   Q7Q6A7   A0A182RZP9   A0A182TR92   A0A182UTE1   A0A182QIT8   A0A182SFD0   Q86PM2   A0A182I1X5   T1DNM2   A0A182NIN3   A0A182GB70   A0A2K7P6B9   A0A182IX05   A0A182YD21   A0A182WKQ4   W5JA14   Q8ISJ5   A0A182FH06   B0XCN2   A0A182MTN1   A0A182TR63   A0A182GP36   A0A182X709   A0A1Q3FQY7   A0A182KPN6   U5EKF9   A0A1J1IT62   A0A1B0DR24   A0A1B6LYM2   A0A212EIH6   A0A1L8DZZ5   A0A0P5K040   A0A0P5ZMC5   A0A190EF52   D6W8X0   W8AKN7   A0A336K959   A0A164XEN7   A0A1B6D6B2   A0A0A1X5Y3   A0A1Z5KUA2   A0A232ES08   A0A0A1XJW2   A0A034W1L3   A0A034W022   A0A2R5L6J7   E9H0N5   Q95NP6   A0A1L8DZW6   A0A0K8VXF8   A0A0K8W6G4   A0A023GK71   B1B6U6   A0A1E1XET2   K7IYV7   P0CU30   A0A0P5ED04   A0A1B6F389   A0A293LEA3   A0A2P2I3X1   A0A1B0EXM3   A0A0P4YVR0   A0A1I8MLQ5   A0A1I8PJE6   A0A0P6H6W1   E0VU04   A0A131YYP6   A0A0L0CJS0   Q86PM2-2   A0A224Z2L3   A0A0L0C2Z9   A0A224YU54   B4J4Y6   A0A3B0JNY3   B5DZB4   A0A1A9WJY5   A0A132AFB7   B4HR71   V5HHY6  

PDB

5X68     E-value=6.28666e-107,     Score=991

KEGG

692509    K00486 kynurenine 3-monooxygenase [EC:1.14.13.9] | (RefSeq) Kmo; kynurenine 3-monooxygenase

PATHWAY

00380    Tryptophan metabolism - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0016021   GO:0004502   GO:0019805   GO:0031966   GO:0006569   GO:0043420   GO:0034354   GO:0071949   GO:0004497   GO:0050660   GO:0019674   GO:0070189   GO:0016174   GO:0005741   GO:0005739   GO:0045454   GO:0016491   GO:0008270   GO:0005216   GO:0006811   GO:0006541   GO:0016812   GO:0016597  

Subcellular location

Mitochondrion  
Membrane  

Length:

456

Number of predicted TMHs:

2

Exp number of AAs in TMHs:

44.82176

Exp number, first 60 AAs:

7.52353999999999

Total prob of N-in:

0.32158

outside

1  -  392

TMhelix

393  -  415

inside

416  -  427

TMhelix

428  -  449

outside

450  -  456

 

 

Pi

192.74721

Theta

17.733667

Tajima's D

0.527355

CLR

0.183679

CSRT

0.531923403829809

Interpretation

Uncertain