Gene

KWMTBOMO05756

Pre Gene Modal

BGIBMGA006887

Annotation

putative_Methylosome_protein_[Danaus_plexippus]

Location

Bomo_Chr10(+):9427785-9438661      View in EpiBrowser

Full name

Methylosome protein 50      

Alternative Name

WD repeat-containing protein 77

Location in the cell

Cytoplasmic   Reliability : 3.038

CDS

ATGTCAGTAGCAGAGCATGATGACTCTATTATGGCTATAGACTGTTTGGAACCTGGCTTTGAATATGTGACAGTAGGGGCTGATGGGAATGCAAAGATATGGGATGTGAAAGAGACGACTTGTGTTAGAAATTACAAGGCAGCACACAGTAAACAAATATATGGAGTATCAGTTCGGCCAAAATCAACAACAACATTTGCCACAGCATCTATGGATGAATATATGTCTTTGTGGGATGAGAATATTGATAAAGCTGTTTTAGATATAACAAAGAATGACTGCAGTATCCGATGTTTGCAGTGGGTAGATGAAAACAAATTACTTTTTGGAGATGAAGCAGGAATGTTATATTCAGTAGATATTCGATATGTGGACTCCACACTGAAATTATTTGAATTTCCAGCAGCTGTTCACAAAATTTTCCTCAATCCTGAGAATGACAAAGTAGCAGTATGCTGTGATAATACAACATTGACAGTATGCAATATACCAGTGGATGACAAAGCTAAAATAATTTACCGCAACAACTCGCACACTAACTTAGTGAGAGGAGTTGCTTGGGATACAAACGAGAGAAAACTTCATTCAGTCGGATGGGATGGAACTGTTAAATCACACAATCTTCCATCAGATTAG

Protein

MSVAEHDDSIMAIDCLEPGFEYVTVGADGNAKIWDVKETTCVRNYKAAHSKQIYGVSVRPKSTTTFATASMDEYMSLWDENIDKAVLDITKNDCSIRCLQWVDENKLLFGDEAGMLYSVDIRYVDSTLKLFEFPAAVHKIFLNPENDKVAVCCDNTTLTVCNIPVDDKAKIIYRNNSHTNLVRGVAWDTNERKLHSVGWDGTVKSHNLPSD

Description

Non-catalytic component of the methylosome complex, composed of PRMT5, WDR77 and CLNS1A, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The methylosome complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.

Subunit

Component of the methylosome complex composed of PRMT5, WDR77 and CLNS1A (By similarity). Found in a complex composed of PRMT5, WDR77 and RIOK1 (By similarity). RIOK1 and CLNS1A bound directly to PRMT5 at the same binding site, in a mutually exclusive manner, which allows the recruitment of distinct methylation substrates, such as nucleolin/NCL and Sm proteins, respectively (By similarity). Found in a complex wit the component of the methylosome, PRMT5, CLNS1A, WDR77, PRMT1 and ERH. Directly interacts with PRMT5, as well as with several Sm proteins, including SNRPB and SNRPD2 and, more weakly, SNRPD3 and SNRPE. Forms a compact hetero-octamer with PRMT5, decorating the outer surface of a PRMT5 tetramer. Interacts with SUZ12 and histone H2A/HIST2H2AC, but not with histones H2B, H3 nor H4. Interacts with CTDP1 and LSM11. Interacts with APEX1, AR and NKX3-1. Interacts with CHTOP.

Keywords

Complete proteome   Cytoplasm   Direct protein sequencing   Nucleus   Phosphoprotein   Reference proteome   Repeat   WD repeat  

Feature

chain  Methylosome protein 50

Uniprot

A0A212FAL4   S4NTZ2   A0A2H1WH14   A0A2A4JQJ4   A0A2W1C358   A0A194Q9Z9   + More
A0A194RCL6   E0V9W0   A0A0L7R976   F4WV27   A0A154PLA0   A0A151IAU4   A0A151IRK0   A0A195EX87   A0A158NNR6   A0A195BT28   A0A0V0GBV6   A0A151WT03   A0A1S3IQD0   A0A067RJA8   A0A0C9RBX1   A0A2J7RK85   A0A2J7RKB1   A0A232F3S8   A0A0N1ITA2   A0A2A3EKJ7   A0A087ZQE0   A0A1B6KX50   A0A1B6DSS4   E9FSP1   A0A1B6DLS7   A0A146LP39   A0A0P4VN65   T1HNQ7   A0A026W1Z7   A0A0A9Y315   A0A023EX15   A0A161N1L7   A0A1B6JQV3   E2A3F4   A0A1B6GR52   A0A224Z4B7   A0A087UBC7   A0A2U3W0C0   Q5ZLD5   A0A131YQV1   A0A1D5PD41   H0YTA4   A0A2Y9IXF8   A0A091HD56   A0A3Q7P3J3   U3JD17   A0A093PWQ2   W8BHQ6   A0A3Q7R209   A0A091VZC0   F6S293   A0A250YG30   A0A164LVV8   A0A0P6DTK1   U6DWS2   A0A099ZAD5   F7FVZ4   A0A093I3M6   A0A0P5D448   A0A2R7VWZ7   D2HXF1   A0A293L5M9   F7BHM5   A0A218UGF6   A0A3L8S3Z6   A0A3L7IFX0   A0A1V4KXI5   A0A2Y9H285   A0A2D4L114   A0A1E1XAG5   A0A1S4E7W0   A0A2D4FW27   A0A384D5P8   A0A0P4XGY8   A0A0P5FGA6   M3W4G4   A0A2K6SW77   A0A3B1K9B9   G9KXX6   E2B7J7   M3Y067   Q7SZR9   A0A0B7A8P3   A0A3Q7TU09   A0A131Y318   A0A0N8AZR4   B7PD08   Q4QR85   A0A1V9ZJE3   E3TDJ1   E3TE22   G1LFT7  

Pubmed

22118469    23622113    28756777    26354079    20566863    21719571    + More
21347285    24845553    28648823    21292972    26823975    27129103    24508170    30249741    25401762    25474469    20798317    28797301    15642098    26830274    15592404    20360741    24495485    18464734    28087693    17495919    20010809    30282656    29704459    28503490    24813606    17975172    25329095    23236062    15489334    25527045    20634964   

EMBL

AGBW02009454    OWR50768.1    GAIX01012026    JAA80534.1    ODYU01008299    SOQ51764.1    + More
NWSH01000887    PCG73680.1    KZ149900    PZC78593.1    KQ459302    KPJ01815.1    KQ460398    KPJ15020.1    DS234996    EEB10166.1    KQ414627    KOC67425.1    GL888384    EGI61915.1    KQ434938    KZC12058.1    KQ978169    KYM96599.1    KQ981124    KYN09326.1    KQ981953    KYN32494.1    ADTU01021736    KQ976417    KYM89828.1    GECL01001282    JAP04842.1    KQ982766    KYQ50976.1    KK852598    KDR20539.1    GBYB01014078    GBYB01014079    GBYB01014080    GBYB01014081    JAG83845.1    JAG83846.1    JAG83847.1    JAG83848.1    NEVH01002981    PNF41240.1    PNF41239.1    NNAY01001115    OXU25093.1    KQ435867    KOX70252.1    KZ288223    PBC32014.1    GEBQ01023954    JAT16023.1    GEDC01008578    JAS28720.1    GL732524    EFX89791.1    GEDC01010681    JAS26617.1    GDHC01010113    JAQ08516.1    GDKW01002671    JAI53924.1    ACPB03010543    KK107485    QOIP01000006    EZA50092.1    RLU21937.1    GBHO01019674    GBRD01004684    GDHC01002867    JAG23930.1    JAG61137.1    JAQ15762.1    GBBI01004969    JAC13743.1    GEMB01002138    JAS01042.1    GECU01006116    JAT01591.1    GL436428    EFN72018.1    GECZ01004899    JAS64870.1    GFPF01009714    MAA20860.1    KK119091    KFM74666.1    AJ719799    CAG31458.1    GEDV01006923    JAP81634.1    AADN05000168    ABQF01054233    KL532828    KFO93384.1    AGTO01000407    KL671007    KFW80871.1    GAMC01013774    GAMC01013772    JAB92781.1    KL410242    KFQ94923.1    GFFW01002230    JAV42558.1    LRGB01003123    KZS04482.1    GDIQ01087510    JAN07227.1    HAAF01014685    CCP86507.1    KL891431    KGL78741.1    KL214740    KFV61341.1    GDIP01161242    JAJ62160.1    KK854142    PTY12027.1    GL193614    EFB15196.1    GFWV01002187    MAA26917.1    MUZQ01000325    OWK52736.1    QUSF01000065    RLV96922.1    RAZU01000058    RLQ77043.1    LSYS01001493    OPJ89110.1    IACL01108490    LAB14645.1    GFAC01002925    JAT96263.1    IACJ01098533    IACJ01098534    LAA51658.1    GDIP01243258    JAI80143.1    GDIQ01255519    JAJ96205.1    AANG04003279    JP021157    AES09755.1    GL446181    EFN88372.1    AEYP01073830    BC056278    BC066433    AAH56278.1    AAH66433.1    HACG01030283    CEK77148.1    GEFM01002539    JAP73257.1    GDIQ01226948    GDIQ01226947    JAK24778.1    ABJB010420628    ABJB010798632    DS687722    EEC04480.1    BC097367    JNBR01000090    OQR98105.1    GU588421    ADO28377.1    GU588602    ADO28558.1    ACTA01130915   

Proteomes

UP000007151    UP000218220    UP000053268    UP000053240    UP000009046    UP000053825    + More
UP000007755    UP000076502    UP000078542    UP000078492    UP000078541    UP000005205    UP000078540    UP000075809    UP000085678    UP000027135    UP000235965    UP000215335    UP000053105    UP000242457    UP000005203    UP000000305    UP000015103    UP000053097    UP000279307    UP000000311    UP000054359    UP000245340    UP000000539    UP000007754    UP000248482    UP000286641    UP000016665    UP000053258    UP000286640    UP000053283    UP000002279    UP000076858    UP000053641    UP000002280    UP000053875    UP000197619    UP000276834    UP000273346    UP000190648    UP000248481    UP000079169    UP000261680    UP000291021    UP000011712    UP000233220    UP000018467    UP000008237    UP000000715    UP000286642    UP000001555    UP000002494    UP000243579    UP000221080    UP000008912   

PRIDE

Q7SZR9    Q4QR85   

Pfam

PF00400   WD40

Interpro

IPR036322   WD40_repeat_dom_sf        + More
IPR017986   WD40_repeat_dom       
IPR001680   WD40_repeat       
IPR015943   WD40/YVTN_repeat-like_dom_sf       
IPR019775   WD40_repeat_CS       
IPR020472   G-protein_beta_WD-40_rep       

SUPFAM

SSF50978   SSF50978       

Gene 3D

2.130.10.10    

ProteinModelPortal

A0A212FAL4   S4NTZ2   A0A2H1WH14   A0A2A4JQJ4   A0A2W1C358   A0A194Q9Z9   + More
A0A194RCL6   E0V9W0   A0A0L7R976   F4WV27   A0A154PLA0   A0A151IAU4   A0A151IRK0   A0A195EX87   A0A158NNR6   A0A195BT28   A0A0V0GBV6   A0A151WT03   A0A1S3IQD0   A0A067RJA8   A0A0C9RBX1   A0A2J7RK85   A0A2J7RKB1   A0A232F3S8   A0A0N1ITA2   A0A2A3EKJ7   A0A087ZQE0   A0A1B6KX50   A0A1B6DSS4   E9FSP1   A0A1B6DLS7   A0A146LP39   A0A0P4VN65   T1HNQ7   A0A026W1Z7   A0A0A9Y315   A0A023EX15   A0A161N1L7   A0A1B6JQV3   E2A3F4   A0A1B6GR52   A0A224Z4B7   A0A087UBC7   A0A2U3W0C0   Q5ZLD5   A0A131YQV1   A0A1D5PD41   H0YTA4   A0A2Y9IXF8   A0A091HD56   A0A3Q7P3J3   U3JD17   A0A093PWQ2   W8BHQ6   A0A3Q7R209   A0A091VZC0   F6S293   A0A250YG30   A0A164LVV8   A0A0P6DTK1   U6DWS2   A0A099ZAD5   F7FVZ4   A0A093I3M6   A0A0P5D448   A0A2R7VWZ7   D2HXF1   A0A293L5M9   F7BHM5   A0A218UGF6   A0A3L8S3Z6   A0A3L7IFX0   A0A1V4KXI5   A0A2Y9H285   A0A2D4L114   A0A1E1XAG5   A0A1S4E7W0   A0A2D4FW27   A0A384D5P8   A0A0P4XGY8   A0A0P5FGA6   M3W4G4   A0A2K6SW77   A0A3B1K9B9   G9KXX6   E2B7J7   M3Y067   Q7SZR9   A0A0B7A8P3   A0A3Q7TU09   A0A131Y318   A0A0N8AZR4   B7PD08   Q4QR85   A0A1V9ZJE3   E3TDJ1   E3TE22   G1LFT7  

PDB

4G56     E-value=2.72855e-14,     Score=188

KEGG

101745075    K13221 methylosome protein 50 | (RefSeq) methylosome protein 50-like

GO

GO:0034709   GO:0006357   GO:0045943   GO:0060528   GO:0008327   GO:0008284   GO:0005654   GO:0006364   GO:0005829   GO:0060770   GO:0005794   GO:0005730   GO:0030374   GO:0004402   GO:0005737   GO:0005634   GO:0005515   GO:0016226   GO:0006508   GO:0004672   GO:0005524   GO:0004568   GO:0006032   GO:0006541   GO:0016812   GO:0016597  

Subcellular location

Nucleus  
Cytoplasm  

Length:

211

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00276

Exp number, first 60 AAs:

0.00248

Total prob of N-in:

0.06622

outside

1  -  211

 

 

Pi

260.908459

Theta

214.288492

Tajima's D

0.897499

CLR

0.047585

CSRT

0.625168741562922

Interpretation

Uncertain