Gene

KWMTBOMO05674  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA006837

Annotation

NADPH_cytochrome_P450_reductase_[Bombyx_mori]

Location

Bomo_Chr10(-):7829094-7858347      View in EpiBrowser

Full name

NADPH--cytochrome P450 reductase      

Location in the cell

Cytoplasmic   Reliability : 1.998 ER   Reliability : 2.192

CDS

ATGTCAGAAAATGCACAAGACTTACTGAAAGATGCAGCTGCCGGTGCCGCCGCTGCAGCCGCTGCTGGTGGATCACTATTCAGCACATTTGACATAATTGTGTTGGCATTGTTGTTGGGAGGTACTATATGGTGGTTTTACAATTCAAAAAAAGAAAGCAAGCGGGACAAAATACTTCTGGAAAAATACTCAATTCAAGCAGCAGGATCTATACAAGTCACAGAAAACTCTTTTATAACAAAATTAAAGACTTCGGGAAGGAGTTTAGTAGTGTTCTATGGATCTCAGACGGGTACTGCGGAAGAATTCGCTGGTCGACTTGCCAAAGAAGGCATGCGCTATAAAATGAAGGGTATGGTGGCTGATCCAGAAGAATGTGATATGGAAGAACTCACAAAGCTACAAGAAATTGAAAACTCCCTGGCTGTTTTCTGTATGGCAACATACGGTGAGGGAGACCCCACAGATAATTCAATGGAATTTTATGAATGGTTAAAAAATCAAGATCCAGATCTGACTGGCCTAAATTACGCAGTATTTGGGCTTGGTAATAAAACATATGAGCATTATAATGCAGTGGCCATTTATTTAGATAAAAGATTAGAAGAACTTGGAGCTACACGAGTATATGAATTGGGAATGGGTGATGATGATGCTAATATTGAAGATGACTTCATTACGTGGAAAGATAAATTTTGGCCAGCGGTATGTGAGAAATACAACATTGAAAGTGCAGGAGAAGAAGAACTCACGAGACAGTTCCGTCACGTGCTCCATCCACCTGATGAAGTTTCTCCTAACAGTGTGTTCACTGGTGAAATTGCTAAGCTACACTCGCTACAAGTTCAAAGACCACCATATGATTCTAAAAATCCATTCTTGGCACAGATTACTGTCAACAGAGAACTACATCGTGGTGGTGACAGATCATGTTTGCATGTAGAATTAGACATATCTGATTCTAAAATGCGATATGAAGCTGGTGATCATGTAGCTGTGTATCCTATCAACGATATGGATTTAGTAGAGCGCTTGGGACAACTGACAAATGCCAATCTCGATGAAATATTTTCGCTTATCAACACTGACCAGGAAAGCAGCAAGAAACATCCATTCCCTTGTCCAACATCTTACAGAACTGCTCTCTCGCATTATGTAGAAATTACAGCATTACCGCGTACCCACATCCTCCGAGAGTTGGTTGAATATTGTTCTAATGAAGAAGATAAGAAGAAGCTGCTTCTGATGGCCACAAACACTCAAGAGGGAAAATCCCTGTATCAGTCGTTTGTAGTTGATGCTTGTAGAAATATTGTGCACATATTGGAAGACCTACCGTCATGTAAACCTCCCTTGGATCATATTTGTGAACTTTTGCCCCGGTTACAACCAAGATACTATTCGATTTCTTCTAGTCCTAAGCTTCATCCAGAGACGGTGCACATTACTGCGGTCGTCGTAAAGTACAAGACTCCGACCGGACGAATCAACAAAGGTGTCGCTACGACGTGGCTTGCTCAACATAAACCCGAGCCGGGGAAACCACTGCCTCGTGTCCCGGTTTATATAAGGAAATCTCAGTTCAGGCTTCCAATGCAGACCCAAACACCAATAATAATGGTGGGACCGGGGACTGGATTGGCGCCCTTCCGAGGTTTTCTTCAAGACCGCGCATTCGTCCGCGCCAGTGGGAAAGAAGTGGGCGAGAACATCCTTTACTTTGGATGCAGACATCGTGATGAGGATTACATCTACCAAGAGGAACTAGAGGAATACGAAAAGAATGGTGACGTAAAACTGAACTTAGCATTTTCACGCGACCAAGCGAACAAAGTTTACGTTACACATTTACTCGAAAATAATATGGACGAACTATGGGATGTTATCGGAAATAGGAATGGAAATTTCTACATTTGCGGTGACGCGAAAAACATGGCTGTGGACGTAAGGAACATTGTGTTGAAAGCTATAAAAGAGAAAGGCGGCCGCACAGAGACGGAGGCCGTGCAGTTCCTCAAGAAGTTAGAATCCGTCAAGAAATATTCTTCGGACGTTTGGAGTTAA

Protein

MSENAQDLLKDAAAGAAAAAAAGGSLFSTFDIIVLALLLGGTIWWFYNSKKESKRDKILLEKYSIQAAGSIQVTENSFITKLKTSGRSLVVFYGSQTGTAEEFAGRLAKEGMRYKMKGMVADPEECDMEELTKLQEIENSLAVFCMATYGEGDPTDNSMEFYEWLKNQDPDLTGLNYAVFGLGNKTYEHYNAVAIYLDKRLEELGATRVYELGMGDDDANIEDDFITWKDKFWPAVCEKYNIESAGEEELTRQFRHVLHPPDEVSPNSVFTGEIAKLHSLQVQRPPYDSKNPFLAQITVNRELHRGGDRSCLHVELDISDSKMRYEAGDHVAVYPINDMDLVERLGQLTNANLDEIFSLINTDQESSKKHPFPCPTSYRTALSHYVEITALPRTHILRELVEYCSNEEDKKKLLLMATNTQEGKSLYQSFVVDACRNIVHILEDLPSCKPPLDHICELLPRLQPRYYSISSSPKLHPETVHITAVVVKYKTPTGRINKGVATTWLAQHKPEPGKPLPRVPVYIRKSQFRLPMQTQTPIIMVGPGTGLAPFRGFLQDRAFVRASGKEVGENILYFGCRHRDEDYIYQEELEEYEKNGDVKLNLAFSRDQANKVYVTHLLENNMDELWDVIGNRNGNFYICGDAKNMAVDVRNIVLKAIKEKGGRTETEAVQFLKKLESVKKYSSDVWS

Description

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
This enzyme is required for electron transfer from NADP to cytochrome p450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome b5 (By similarity). May function to clear the olfactory organ (antennae) from accumulating chemicals.
This enzyme is required for electron transfer from NADP to cytochrome P450.

Catalytic Activity

NADPH + 2 oxidized [cytochrome P450] = H(+) + NADP(+) + 2 reduced [cytochrome P450]

Cofactor

FAD
FMN

Similarity

Belongs to the NADPH--cytochrome P450 reductase family.
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
In the N-terminal section; belongs to the flavodoxin family.

Keywords

Complete proteome   Endoplasmic reticulum   FAD   Flavoprotein   FMN   Membrane   NADP   Oxidoreductase   Transmembrane   Transmembrane helix   Reference proteome  

Feature

chain  NADPH--cytochrome P450 reductase

Uniprot

Q9NKV3   A0FGR6   H9JBE2   E0A3A7   Q5YJK3   F1DI27   + More
J7FJF0   A0A1S5ZY34   R4UB73   A0A212EIJ2   A0A2S0RQR2   A0A2H1WF35   A0A346M705   A0A194PER0   A0A194QL54   A0A0B4N5E5   S4PB72   A0A023EXK0   Q17FM7   B8Y644   A0A023EUS9   A0A336MHB7   A0A1L8E0M8   A0A1Q3FJ57   A0A0L0C865   B4LQV6   A0A068FC77   A0A1B0DP66   A0A0M5IYG0   A0A034WP73   A0A0A1XSJ7   A9PL55   A0A182RH76   A0A2A4IYH3   A0A385L4D0   A0A182TPA1   B0X829   A0A182XDZ7   D3TNM8   A0A182V5Z6   A0A182HGM6   B3MKG0   A0A1B0GK97   A0A0K8WAU2   A0A0K8TSW9   B4MUS1   Q86QH5   A0A182PFV5   B4JBQ6   A0A0K1YWC3   A0A182JWU0   A0A1J1IFV3   D6X2B1   T1P8B4   W8BJA3   Q07994   B4KFY5   U5EZ84   A0A182W4I2   A0A1A9WZ84   A0A182MTU0   A0A182MZJ4   A0A0I9QPP6   A0A1A9UHQ5   A0A2M4BG65   A0A0J9QWX4   B4I1U1   A0A1B0ABZ7   A0A0J9TGX6   B3N5I0   M9PCQ1   Q27597   A0A182Q802   A4ZIT2   A0A084VVL2   B4P0F6   A0A2M3YYK4   A0A2M3ZFH9   A0A1A9Y1E3   A0A1B0ARY9   A0A182G004   A0A182IXF4   A0A1W4VA10   I1VJ18   W5J6Q0   B4Q4L3   N6T5K3   A0A2M4A4Y4   Q29JN6   B4GJE2   A0A1Y1LBM5   A0A3B0JVM8   A0A0M3R7Y6   A0A3S2M7C9   A0A2H4BK51   A0A1L2EC44   A0A2M4A547   X2F552   A0A068EUU1   X2F6A7  

EC Number

1.6.2.4

Pubmed

11082204    19121390    22689565    24768738    23512641    15716002    + More
22984814    24036507    22118469    26354079    25550052    23622113    24945155    17510324    26108605    17994087    25880816    25348373    25830018    20353571    26369729    14585502    18362917    19820115    24495485    8508186    25244985    25368081    22936249    18057021    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    26109357    26109356    9168130    12537569    17598560    24438588    17550304    22516182    23537049    24139909    20920257    23761445    15632085    28004739    26319543   

EMBL

AB042615    BAA95684.1    DQ993199    ABJ97709.1    BABH01030817    BABH01030818    + More
HM347785    HQ116527    KF555286    KF555287    KF419215    KF419216    ADK25060.1    ADM45306.1    AGZ82642.1    AGZ82643.1    AY390257    AAR26515.1    HQ852049    ADX95746.1    JX310073    AFP20584.1    KY609859    AQR00227.1    KC689343    AGM20565.1    AGBW02014631    OWR41297.1    MF991880    AWA45974.1    ODYU01007901    SOQ51084.1    MG018925    AXQ06577.1    KQ459606    KPI91508.1    KQ461198    KPJ06109.1    KJ767015    AIJ00849.1    GAIX01004731    JAA87829.1    GAPW01000409    JAC13189.1    CH477269    EAT45397.1    FJ487605    ACL01092.1    GAPW01000408    JAC13190.1    UFQT01001084    SSX28811.1    GFDF01001837    JAV12247.1    GFDL01007491    JAV27554.1    JRES01000772    KNC28440.1    CH940649    EDW63490.1    KJ702307    AID61461.1    AJVK01007926    CP012523    ALC38386.1    GU325631    GAKP01002962    GAKP01002959    ADO24532.2    JAC55990.1    GBXI01016016    GBXI01000330    JAC98275.1    JAD13962.1    EF152578    ABO77954.1    NWSH01005003    PCG64506.1    MH512105    AYA22617.1    DS232472    EDS42298.1    EZ423030    ADD19306.1    APCN01005677    CH902620    EDV31513.1    AJWK01028576    AJWK01028577    GDHF01004140    JAI48174.1    GDAI01000146    JAI17457.1    CH963857    EDW76266.1    AY183375    AAO24765.1    CH916368    EDW02991.1    KP859405    AKZ17715.1    CVRI01000047    CRK97329.1    KQ971371    EFA10239.1    KA644852    AFP59481.1    GAMC01005186    GAMC01005185    JAC01371.1    L19897    CH933807    EDW12111.1    GANO01001253    JAB58618.1    AXCM01002344    BK008720    DAA64796.1    GGFJ01002876    MBW52017.1    CM002910    KMY88498.1    CH480820    EDW54498.1    KMY88495.1    CH954177    EDV59059.1    AE014134    AGB92665.1    X93090    AY052000    AXCN02001282    EF057735    ABL75156.1    ATLV01017197    KE525157    KFB42006.1    CM000157    EDW87914.1    GGFM01000585    MBW21336.1    GGFM01006508    MBW27259.1    JXJN01002620    AXCP01007993    AXCP01007994    JQ855639    KB632293    AFI45002.1    ERL91636.1    ADMH02001968    ETN60137.1    CM000361    EDX03940.1    APGK01043474    APGK01043475    KB741014    ENN75479.1    GGFK01002461    MBW35782.1    CH379062    EAL32925.1    CH479184    EDW37456.1    GEZM01060382    JAV71024.1    OUUW01000010    SPP86155.1    KT004539    ALC78506.1    RSAL01000019    RVE52696.1    KF984040    AHJ59373.1    KT033858    ANJ65959.1    GGFK01002613    MBW35934.1    KJ017970    AHM93009.1    KJ668698    AID55422.1    KJ017971    AHM93010.1   

Proteomes

UP000005204    UP000007151    UP000053268    UP000053240    UP000008820    UP000037069    + More
UP000008792    UP000092462    UP000092553    UP000075900    UP000218220    UP000075902    UP000002320    UP000076407    UP000075903    UP000075840    UP000007801    UP000092461    UP000007798    UP000075885    UP000001070    UP000075881    UP000183832    UP000007266    UP000095301    UP000009192    UP000075920    UP000091820    UP000075883    UP000075884    UP000076408    UP000078200    UP000001292    UP000092445    UP000008711    UP000000803    UP000075886    UP000030765    UP000002282    UP000092443    UP000092460    UP000069272    UP000075880    UP000192221    UP000030742    UP000000673    UP000000304    UP000019118    UP000001819    UP000008744    UP000268350    UP000283053   

PRIDE

Q9NKV3    H9JBE2    M9PCQ1    Q27597   

Pfam

PF00258   Flavodoxin_1        + More
PF00667   FAD_binding_1
PF00175   NAD_binding_1

Interpro

IPR017938   Riboflavin_synthase-like_b-brl        + More
IPR008254   Flavodoxin/NO_synth       
IPR001433   OxRdtase_FAD/NAD-bd       
IPR023173   NADPH_Cyt_P450_Rdtase_alpha       
IPR017927   FAD-bd_FR_type       
IPR029039   Flavoprotein-like_sf       
IPR001094   Flavdoxin-like       
IPR023208   P450R       
IPR039261   FNR_nucleotide-bd       
IPR001709   Flavoprot_Pyr_Nucl_cyt_Rdtase       
IPR003097   CysJ-like_FAD-binding       

SUPFAM

SSF52218   SSF52218        + More
SSF63380   SSF63380       
SSF52343   SSF52343       

Gene 3D

1.20.990.10     3.40.50.360     3.40.50.80    

ProteinModelPortal

Q9NKV3   A0FGR6   H9JBE2   E0A3A7   Q5YJK3   F1DI27   + More
J7FJF0   A0A1S5ZY34   R4UB73   A0A212EIJ2   A0A2S0RQR2   A0A2H1WF35   A0A346M705   A0A194PER0   A0A194QL54   A0A0B4N5E5   S4PB72   A0A023EXK0   Q17FM7   B8Y644   A0A023EUS9   A0A336MHB7   A0A1L8E0M8   A0A1Q3FJ57   A0A0L0C865   B4LQV6   A0A068FC77   A0A1B0DP66   A0A0M5IYG0   A0A034WP73   A0A0A1XSJ7   A9PL55   A0A182RH76   A0A2A4IYH3   A0A385L4D0   A0A182TPA1   B0X829   A0A182XDZ7   D3TNM8   A0A182V5Z6   A0A182HGM6   B3MKG0   A0A1B0GK97   A0A0K8WAU2   A0A0K8TSW9   B4MUS1   Q86QH5   A0A182PFV5   B4JBQ6   A0A0K1YWC3   A0A182JWU0   A0A1J1IFV3   D6X2B1   T1P8B4   W8BJA3   Q07994   B4KFY5   U5EZ84   A0A182W4I2   A0A1A9WZ84   A0A182MTU0   A0A182MZJ4   A0A0I9QPP6   A0A1A9UHQ5   A0A2M4BG65   A0A0J9QWX4   B4I1U1   A0A1B0ABZ7   A0A0J9TGX6   B3N5I0   M9PCQ1   Q27597   A0A182Q802   A4ZIT2   A0A084VVL2   B4P0F6   A0A2M3YYK4   A0A2M3ZFH9   A0A1A9Y1E3   A0A1B0ARY9   A0A182G004   A0A182IXF4   A0A1W4VA10   I1VJ18   W5J6Q0   B4Q4L3   N6T5K3   A0A2M4A4Y4   Q29JN6   B4GJE2   A0A1Y1LBM5   A0A3B0JVM8   A0A0M3R7Y6   A0A3S2M7C9   A0A2H4BK51   A0A1L2EC44   A0A2M4A547   X2F552   A0A068EUU1   X2F6A7  

PDB

5FA6     E-value=0,     Score=1999

KEGG

100127056    K00327 NADPH-ferrihemoprotein reductase [EC:1.6.2.4] | (RefSeq) Cpr, bCPR; NADPH cytochrome P450 red

GO

GO:0016021   GO:0005789   GO:0010181   GO:0003958   GO:0050660   GO:0050661   GO:0016491   GO:0006723   GO:0009725   GO:0005829   GO:0012505   GO:0008270   GO:0043565   GO:0003677   GO:0004887   GO:0043401   GO:0003707   GO:0055114  

Subcellular location

Endoplasmic reticulum membrane  

Length:

687

Number of predicted TMHs:

1

Exp number of AAs in TMHs:

26.54232

Exp number, first 60 AAs:

25.44252

Total prob of N-in:

0.89604

POSSIBLE N-term signal

sequence

inside

1  -  24

TMhelix

25  -  47

outside

48  -  687

 

 

Pi

197.824459

Theta

180.188055

Tajima's D

0.426919

CLR

0.780714

CSRT

0.489875506224689

Interpretation

Uncertain