Gene

KWMTBOMO05544

Pre Gene Modal

BGIBMGA006602

Annotation

PREDICTED:_poly(A)-specific_ribonuclease_PARN-like_domain-containing_protein_1_[Papilio_polytes]

Location

Bomo_Chr10(-):4466448-4468287      View in EpiBrowser

Full name

Pre-piRNA 3'-exonuclease trimmer      

Alternative Name

PARN-like domain-containing protein 1 homolog

Location in the cell

Nuclear   Reliability : 2.231

CDS

ATGGATATCACCAAAGAAAACTATTATGAAGAACTTGATAACATAGCAAAAAATATAAAAGACTCGTGTTTTATAAGTTTTGATGCAGAGTTTTCAGCTATACTTACCAAAGAATCGTTCAAATACAGCTTGTTCGACACAAATGAAGAAAGATACAATAAGTACAAAACACAAATAAGCACAATGAAAATGATGCAAGTAGGACTGACTATGTTTCAATATGATAGAGAATTAGATGCATACCTTGCAACTGGTTATACATTTCATTTGTGTCCACAACTAATTGGAACGATAAATCAGTCGCTCATATTTCAAGCCTCAACCCTCAAGTTTTTATGTAAACACAATTTCGATTTCAATAAGTTCATCTATGAAGGCCTTCCATATCTTAGCAAGAGTGATGAGGCATTGTTAAACAGATACAGAATAGAAAACAACCTGTTTGACTATGTCAGTGAGTCTCTTGAATTTGATGAAGAGAAGCAAATCAACCAATGTTCTTCAGAAGTTTCAAAATGGTTGTCATCGAGTATAGAGGACACAATGTACTTAGATATTGACAATGCTATTTGCCGGTACTTGCTGCACTTAGAGCTAAGACAACGTTATCCTGGTATCCTGACTACAGATAGCTTAGGTAATAGCAAGAAGGTAATTTAA

Protein

MDITKENYYEELDNIAKNIKDSCFISFDAEFSAILTKESFKYSLFDTNEERYNKYKTQISTMKMMQVGLTMFQYDRELDAYLATGYTFHLCPQLIGTINQSLIFQASTLKFLCKHNFDFNKFIYEGLPYLSKSDEALLNRYRIENNLFDYVSESLEFDEEKQINQCSSEVSKWLSSSIEDTMYLDIDNAICRYLLHLELRQRYPGILTTDSLGNSKKVI

Description

3'-5' exonuclease that specifically cleaves precursor piRNAs (pre-piRNAs) at their 3' ends (PubMed:26919431). Trims pre-piRNAs to their mature size, a process required for piRNAs maturation and stabilization, and subsequent pre-piRNAs 2'-O-methylation (PubMed:26919431). The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons (PubMed:26919431).

Cofactor

Mg(2+)

Subunit

Interacts with Papi/Tdrkh; interaction takes place on the mitochondrial surface and recruits PNLDC1/trimmer to PIWI-bound pre-piRNAs.

Similarity

Belongs to the CAF1 family.

Keywords

Complete proteome   Exonuclease   Hydrolase   Magnesium   Membrane   Metal-binding   Mitochondrion   Mitochondrion outer membrane   Nuclease   Reference proteome   RNA-binding   RNA-mediated gene silencing   Transmembrane   Transmembrane helix  

Feature

chain  Pre-piRNA 3'-exonuclease trimmer

Uniprot

A0A1U6USN9   H9JAQ7   A0A3S2NZN8   A0A2A4KBR8   A0A194PG21   A0A194QL30   + More
A0A212F817   A0A2H1WWY1   A0A0L7LAH1   A0A2J7R7Q0   A0A067RCE4   A0A2J7R7Q3   A0A2J7R7Q1   A0A2P8XTI4   A0A154PK67   A0A0J7L5J8   A0A3Q0J8T0   E2ANM1   A0A224XIL7   A0A0M9A428   A0A1Z5KY51   A0A2R5LL04   A0A2A3EDY9   A0A1B6F437   E2BNY4   A0A0V0GB39   A0A1B6DB48   A0A1B6CGZ9   A0A0L7R9Y6   A0A1B6HYR9   A0A088ACZ8   A0A023EY28   A0A1S3H980   A0A293LUZ6   A0A1B6LCA4   A0A195CSU0   A0A151IYV7   E0VHV7   A0A151X778   A0A310SUW4   D6WZH5   A0A210QTT4   A0A158NWK4   F4WZ85   V4AS73   A0A195F7S5   A7S4R7   J9JVQ7   A0A177WCI4   F4PBJ7   A0A195AU82   A0A2H8TLU9   A0A3M7T215   A0A224Z6A9   E9I915   A0A1Y1MLM5   A0A026X1W0   A0A3L8DV12   L8HCQ5   A0A3S1A6Y9   A0A131YWV9   A0A1E1XC80   A0A1D2MPM0   A0A1Y1Y108   A0A075AUJ9   N6UPM9   A0A0A9W3L7   T1HJ73   A0A1W4XI64   A0A146LA62   A0A2S2QPV5   B7PS70   A0A3S3SC75   A0A196SFE6   A0A131XSM5   A0A2G8K9D4   A0A2B4RXG8   A0A1E1W4W7   A0A0B6ZFZ7   A0A1B6DFL6   A0A151M2B1   A0A1B6D868   A0A1U7RDZ6   A0A0L0HSE9   A0A1S8W8V7   U4L276   A0A026WYM1   A0A3L8D835   G3MFK5   A0A0Q3USM3   A0A023GNP6   I3M6D0   A0A0P5VX41   A0A3M6TI40   A0A0N7ZN89   A0A2A4JHI6  

EC Number

3.1.13.-

Pubmed

19121390    26919431    26354079    22118469    26227816    24845553    + More
29403074    20798317    28528879    25474469    20566863    18362917    19820115    28812685    21347285    21719571    23254933    17615350    30375419    28797301    21282665    28004739    24508170    30249741    23375108    26830274    28503490    27289101    23932404    23537049    25401762    26823975    29023486    22293439    24068976    22216098    30382153   

EMBL

BABH01008568    BABH01008569    RSAL01000081    RVE48606.1    NWSH01000001    PCG81122.1    + More
KQ459606    KPI91649.1    KQ461198    KPJ06242.1    AGBW02009807    OWR49885.1    ODYU01011676    SOQ57573.1    JTDY01002063    KOB72201.1    NEVH01006727    PNF36862.1    KK852556    KDR21432.1    PNF36863.1    PNF36864.1    PYGN01001374    PSN35310.1    KQ434943    KZC12235.1    LBMM01000487    KMQ98242.1    GL441256    EFN64967.1    GFTR01004090    JAW12336.1    KQ435755    KOX76046.1    GFJQ02006924    JAW00046.1    GGLE01006067    MBY10193.1    KZ288285    PBC29502.1    GECZ01024832    JAS44937.1    GL449511    EFN82576.1    GECL01000966    JAP05158.1    GEDC01014385    JAS22913.1    GEDC01024522    JAS12776.1    KQ414620    KOC67680.1    GECU01027880    JAS79826.1    GBBI01004750    JAC13962.1    GFWV01007803    MAA32533.1    GEBQ01018658    JAT21319.1    KQ977305    KYN03705.1    KQ980735    KYN13751.1    DS235172    EEB12963.1    KQ982450    KYQ56227.1    KQ759903    OAD62005.1    KQ971372    EFA09703.1    NEDP02001925    OWF52139.1    ADTU01028122    GL888470    EGI60474.1    KB201304    ESO97720.1    KQ981756    KYN36114.1    DS469579    EDO41332.1    ABLF02022439    DS022301    OAJ37789.1    GL882892    EGF77459.1    KQ976741    KYM75585.1    GFXV01003332    MBW15137.1    REGN01000429    RNA41987.1    GFPF01010674    MAA21820.1    GL761667    EFZ22924.1    GEZM01027912    JAV86582.1    KK107024    EZA62295.1    QOIP01000004    RLU24257.1    KB007890    ELR22146.1    RQTK01000212    RUS84255.1    GEDV01005110    JAP83447.1    GFAC01002331    JAT96857.1    LJIJ01000726    ODM94983.1    MCFE01000310    ORX91701.1    KE561170    EPZ32187.1    APGK01022442    APGK01022443    APGK01022444    APGK01022445    KB740424    ENN80687.1    GBHO01044144    JAF99459.1    ACPB03017011    GDHC01013405    JAQ05224.1    GGMS01010357    MBY79560.1    ABJB010371410    DS777384    EEC09442.1    NCKU01001410    NCKU01001402    NCKU01001390    RWS12161.1    RWS12176.1    RWS12222.1    LXWW01000183    OAO15046.1    GEFM01006139    JAP69657.1    MRZV01000766    PIK44614.1    LSMT01000243    PFX22311.1    GDQN01009050    JAT82004.1    HACG01019916    CEK66781.1    GEDC01012886    JAS24412.1    AKHW03006780    KYO18664.1    GEDC01015405    JAS21893.1    KQ257451    KND03779.1    LYON01000053    OON10513.1    HF935286    CCX06373.1    KK107078    EZA60239.1    QOIP01000011    RLU16444.1    JO840656    AEO32273.1    LMAW01002292    KQK81594.1    GBBM01000843    JAC34575.1    AGTP01022054    GDIP01094940    JAM08775.1    RCHS01003537    RMX40991.1    GDIP01228825    JAI94576.1    NWSH01001365    PCG71535.1   

Proteomes

UP000005204    UP000283053    UP000218220    UP000053268    UP000053240    UP000007151    + More
UP000037510    UP000235965    UP000027135    UP000245037    UP000076502    UP000036403    UP000079169    UP000000311    UP000053105    UP000242457    UP000008237    UP000053825    UP000005203    UP000085678    UP000078542    UP000078492    UP000009046    UP000075809    UP000007266    UP000242188    UP000005205    UP000007755    UP000030746    UP000078541    UP000001593    UP000007819    UP000077115    UP000007241    UP000078540    UP000276133    UP000053097    UP000279307    UP000011083    UP000271974    UP000094527    UP000193498    UP000030755    UP000019118    UP000015103    UP000192223    UP000001555    UP000285301    UP000078348    UP000230750    UP000225706    UP000050525    UP000189705    UP000053201    UP000189906    UP000018144    UP000051836    UP000005215    UP000275408   

PRIDE

H9JAQ7   

Pfam

PF04857   CAF1        + More
PF08675   RNA_bind
PF00096   zf-C2H2

Interpro

IPR006941   RNase_CAF1        + More
IPR012337   RNaseH-like_sf       
IPR036397   RNaseH_sf       
IPR036867   R3H_dom_sf       
IPR012677   Nucleotide-bd_a/b_plait_sf       
IPR014789   PolyA-riboNase_RNA_binding       
IPR013087   Znf_C2H2_type       
IPR036236   Znf_C2H2_sf       
IPR034042   PARN_R3H       
IPR035979   RBD_domain_sf       
IPR001374   R3H_dom       

SUPFAM

SSF53098   SSF53098        + More
SSF82708   SSF82708       
SSF57667   SSF57667       
SSF54928   SSF54928       

Gene 3D

3.30.1370.50     3.30.420.10     3.30.70.330    

CDD

cd02637  R3H_PARN

ProteinModelPortal

A0A1U6USN9   H9JAQ7   A0A3S2NZN8   A0A2A4KBR8   A0A194PG21   A0A194QL30   + More
A0A212F817   A0A2H1WWY1   A0A0L7LAH1   A0A2J7R7Q0   A0A067RCE4   A0A2J7R7Q3   A0A2J7R7Q1   A0A2P8XTI4   A0A154PK67   A0A0J7L5J8   A0A3Q0J8T0   E2ANM1   A0A224XIL7   A0A0M9A428   A0A1Z5KY51   A0A2R5LL04   A0A2A3EDY9   A0A1B6F437   E2BNY4   A0A0V0GB39   A0A1B6DB48   A0A1B6CGZ9   A0A0L7R9Y6   A0A1B6HYR9   A0A088ACZ8   A0A023EY28   A0A1S3H980   A0A293LUZ6   A0A1B6LCA4   A0A195CSU0   A0A151IYV7   E0VHV7   A0A151X778   A0A310SUW4   D6WZH5   A0A210QTT4   A0A158NWK4   F4WZ85   V4AS73   A0A195F7S5   A7S4R7   J9JVQ7   A0A177WCI4   F4PBJ7   A0A195AU82   A0A2H8TLU9   A0A3M7T215   A0A224Z6A9   E9I915   A0A1Y1MLM5   A0A026X1W0   A0A3L8DV12   L8HCQ5   A0A3S1A6Y9   A0A131YWV9   A0A1E1XC80   A0A1D2MPM0   A0A1Y1Y108   A0A075AUJ9   N6UPM9   A0A0A9W3L7   T1HJ73   A0A1W4XI64   A0A146LA62   A0A2S2QPV5   B7PS70   A0A3S3SC75   A0A196SFE6   A0A131XSM5   A0A2G8K9D4   A0A2B4RXG8   A0A1E1W4W7   A0A0B6ZFZ7   A0A1B6DFL6   A0A151M2B1   A0A1B6D868   A0A1U7RDZ6   A0A0L0HSE9   A0A1S8W8V7   U4L276   A0A026WYM1   A0A3L8D835   G3MFK5   A0A0Q3USM3   A0A023GNP6   I3M6D0   A0A0P5VX41   A0A3M6TI40   A0A0N7ZN89   A0A2A4JHI6  

PDB

3D45     E-value=6.41748e-18,     Score=219

KEGG

101738496    K01148 poly(A)-specific ribonuclease [EC:3.1.13.4] | (RefSeq) poly(A)-specific ribonuclease PARN-lik

PATHWAY

03018    RNA degradation - Bombyx mori (domestic silkworm)

GO

GO:0003676   GO:0046872   GO:1990511   GO:0005739   GO:0005741   GO:0016021   GO:0000175   GO:0031047   GO:0003723   GO:0004527   GO:0004535   GO:0005737   GO:0005783   GO:0000289   GO:0005634   GO:0006402   GO:0001825   GO:0006974   GO:0001664   GO:0005525   GO:0007264   GO:0003824  

Subcellular location

Mitochondrion outer membrane  

Length:

219

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.60494

Exp number, first 60 AAs:

0.00082

Total prob of N-in:

0.24901

outside

1  -  219

 

 

Pi

270.47266

Theta

203.634444

Tajima's D

1.350013

CLR

0

CSRT

0.747612619369032

Interpretation

Uncertain