Gene

KWMTBOMO05324

Pre Gene Modal

BGIBMGA002392

Annotation

PREDICTED:_acid_sphingomyelinase-like_phosphodiesterase_3a_[Papilio_xuthus]

Location

Bomo_Chr9(-):14071687-14083297      View in EpiBrowser

Full name

Acid sphingomyelinase-like phosphodiesterase       + More
Sphingomyelinase phosphodiesterase      
Acid sphingomyelinase-like phosphodiesterase 3a      

Location in the cell

PlasmaMembrane   Reliability : 2.407

CDS

ATGAATTTAAGCAGCTTCGCCCTCTACATCACCAGCCTGTTGTGGCTTAATCTGACAGATGCTAAAATCGGTTACTTTTGGCACATCACAGATCTTCATTACGACCCCTTTCACAACTCCCCGGAGTTTCATAGAGGTTGCAGACACAATAGGGGCAGCAGCGATCACAGCCATCGGAACGGGAGGCATCGTGACCACACCTGCGACAGTTCTTGGCCTTTGATTCAAAGTGCCGCGTCGTTTATGCACGAGCGTCATCCTGAAACCCTCGAATTCGTGTTATGGACTGGGGATATATTATCATCATCCATGGAACACTTGAGTGACGAAATCAAACTGGAAGCCGTCAGAAATGTAACATATGTATTGAGCACAACTTTTAGTAGCCAATTCGTTTTCCCTGCTCTGGGGCACAATGACCCACCGCCCTCGCGCAAACTTGTCGAAATGTGGAGGCAGTGGCTACCCACAGAGGCTTTGCAAACTTTTGAAATCGGAGGATACTACACTATTGAGCAGTCACACAGTAAGCTCAGGATCATAGTTCTGAACAGTGTTCTTTGGGCCGGTGGAGCAGCAAGAACTGACGGACCTAATGTGGGACGAGCTCAGTGGGAGTGGTTAAATCAAGTACTCAGTACAGCTAGAAAGAAAAGCGAAATGGTGTACATAGTTGCCCACGCCGGTCCAGGAGTCGAAGACAGACACAACGCCGGCAGCTCGTCGGCTTCTGGTGGTGGTGAATTGTCTCCGTCTGCTAATGCCAGACTCTTACACATAATTCGATCGTTTAGCGATGTAATCGCTGGACAATTTTACGGGCATCGCCACTCTGATACGTTTAGACTGATTTATAATGAAGGTCATCCGGTTTCATGGGCACTTTTGGCTCCTTCGTTGACTCCGAGAGGTGCGGGCAGCGTTACCAATCCCGGTTTGAGACTTTACAAGTTTGAGATTAACACTGGAAAAGTCTTAGACTACACCCAATATTATTTGGACGTGACCAACCCGAAAGGTGAAATCCATTGGTCTATAGAATACAATCTAACACAATACTACGGGCTGCGAGAGATAAATGCTGCGTCTCTACACGCTTTGGCTGAGAAAATCCGGAGTCATCACGATCGTAGCGTTTTTAATAAGTATTTAACCGCACTACGCGTGCGACATTCGACGGACACGTCTGACTGTGACGCGTCGTGTGCCCACGTTCACTACTGCGCCGTCACGAGGGCCGATTACTCCGAGTTCCGATCGTGTGTCCGGAATCCAGCCTCGGCACTAGCATCACGCGCAGCTCAAAACACAACCGCCATTATTCTGTACGCGATTTTGATTTTTATGTCGTCTTAA

Protein

MNLSSFALYITSLLWLNLTDAKIGYFWHITDLHYDPFHNSPEFHRGCRHNRGSSDHSHRNGRHRDHTCDSSWPLIQSAASFMHERHPETLEFVLWTGDILSSSMEHLSDEIKLEAVRNVTYVLSTTFSSQFVFPALGHNDPPPSRKLVEMWRQWLPTEALQTFEIGGYYTIEQSHSKLRIIVLNSVLWAGGAARTDGPNVGRAQWEWLNQVLSTARKKSEMVYIVAHAGPGVEDRHNAGSSSASGGGELSPSANARLLHIIRSFSDVIAGQFYGHRHSDTFRLIYNEGHPVSWALLAPSLTPRGAGSVTNPGLRLYKFEINTGKVLDYTQYYLDVTNPKGEIHWSIEYNLTQYYGLREINAASLHALAEKIRSHHDRSVFNKYLTALRVRHSTDTSDCDASCAHVHYCAVTRADYSEFRSCVRNPASALASRAAQNTTAIILYAILIFMSS

Description

Has in vitro nucleotide phosphodiesterase activity with nucleoside triphosphates, such as ATP. Has in vitro activity with p-nitrophenyl-TMP. Has lower activity with nucleoside diphosphates, and no activity with nucleoside monophosphates. Has in vitro activity with CDP-choline, giving rise to CMP and phosphocholine. Has in vitro activity with CDP-ethanolamine. Does not have sphingomyelin phosphodiesterase activity.
Has in vitro nucleotide phosphodiesterase activity with nucleoside triphosphates, such as ATP (PubMed:25288789, PubMed:26783088). Has in vitro activity with p-nitrophenyl-TMP (PubMed:25288789). Has lower activity with nucleoside diphosphates, and no activity with nucleoside monophosphates (PubMed:25288789, PubMed:26783088). Has in vitro activity with CDP-choline, giving rise to CMP and phosphocholine. Has in vitro activity with CDP-ethanolamine (PubMed:26783088). Does not have sphingomyelin phosphodiesterase activity (PubMed:25288789, PubMed:26783088).

Cofactor

Zn(2+)

Biophysicochemical Properties

326 uM for ATP
306 uM for ADP
348 uM for ADP-ribose
390 uM for CDP-ethanolamine
262 uM for CDP-choline

Subunit

Monomer.

Similarity

Belongs to the acid sphingomyelinase family.

Keywords

Complete proteome   Disulfide bond   Glycoprotein   Hydrolase   Metal-binding   Reference proteome   Secreted   Signal   Zinc   3D-structure   Alternative splicing   Polymorphism  

Feature

chain  Acid sphingomyelinase-like phosphodiesterase
splice variant  In isoform 2.
sequence variant  In dbSNP:rs12523814.

Uniprot

A0A194QIT8   A0A212ES85   A0A2H1VY23   A0A0N1IQ10   A0A2W1BKX4   A0A2H1W9F8   + More
A0A194QR38   A0A194QCX4   A0A3S2N4T6   A0A139WAW8   A0A212EIY4   H9JEU8   A0A1W4X5I9   A0A067RJ29   A0A2S2QMJ5   A0A088AER8   A0A2H8U085   A0A2A3EAU8   A0A026VYW8   B4N490   B3NGM2   Q8IQD3   Q2LZA0   B3MA63   A0A0M9A977   A0A195F186   A0A0Q9WI47   E0VZL8   A0A151IQE7   A0A1W4U962   Q172E7   A0A151XBZ8   F4X6F8   B4LG96   A0A195B2Q9   B4HAE2   A0A195E6T8   A0A154PQH5   B4J2N4   A0A158NGE5   B4QNP8   E2AMG6   A0A2A4JBL4   A0A0C9QK20   A0A1B6FNW2   A0A2P8XVA8   A0A0P4Y4T5   T1HWJ8   A0A0N8A3F6   A0A0P6DYQ7   A0A0P6HED9   A0A1D2NBQ4   A0A0P5DS43   H0ZNK5   H0XBA3   S7MGZ7   A0A1U7Q910   A0A1S3JCI9   G1PCL0   F8W4E2   G3S0A8   A0A3M0KJ37   G9KQ98   M3YSQ7   A0A2K5E582   A0A2Y9JEP0   U3KA47   G3T666   H2QTP0   I3MMQ9   A0A2B4RQL5   J9NYQ3   Q641Z7   A0A2K5PXL6   G3HKL1   L5JMX2   F7AAZ3   M3WFD8   A0A250Y163   G1SFK1   A0A287D4D5   A0A2R8ZKG5   Q92484   A0A218V436   A0A2U3W670   V9KTZ9   K7GCP6   A0A2K6CAM2   G7P391   G7MQL5   G1RU96   A0A2K5VG13   F6TB74   F1S2T7   L8IWP6   A0A3L7I9Q5  

EC Number

3.1.4.-

Pubmed

26354079    22118469    28756777    18362917    19820115    19121390    + More
24845553    24508170    30249741    17994087    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    26109357    26109356    15632085    23185243    18057021    20566863    17510324    21719571    21347285    20798317    29403074    27289101    20360741    21993624    23594743    22398555    23236062    16136131    16341006    15489334    21804562    23258410    25243066    17975172    28087693    22722832    14702039    14574404    19159218    25288789    26783088    24402279    17381049    22002653    17431167    30723633    22751099    29704459   

EMBL

KQ458714    KPJ05473.1    AGBW02012853    OWR44329.1    ODYU01005151    SOQ45727.1    + More
KQ459930    KPJ19294.1    KZ150019    PZC74931.1    ODYU01007162    SOQ49700.1    KQ461198    KPJ05996.1    KQ459185    KPJ03393.1    RSAL01000531    RVE41444.1    KQ971380    KYB25011.1    AGBW02014553    OWR41446.1    BABH01023327    KK852603    KDR20460.1    GGMS01009708    MBY78911.1    GFXV01008180    MBW19985.1    KZ288301    PBC28838.1    KK107681    QOIP01000006    EZA48054.1    RLU22212.1    CH964095    EDW78964.2    CH954178    EDV51258.2    AE014296    AAN11931.4    ALI30470.1    ALI30471.1    CH379069    EAL29608.3    KRT08182.1    CH902618    EDV39077.2    KPU77861.1    KQ435710    KOX79790.1    KQ981864    KYN34228.1    CH940647    KRF84365.1    KRF84366.1    KRF84367.1    DS235853    EEB18824.1    KQ976780    KYN08356.1    CH477439    EAT40899.1    KQ982314    KYQ57884.1    GL888818    EGI57918.1    EDW69404.2    KQ976662    KYM78577.1    CH479240    EDW37540.1    KQ979568    KYN20910.1    KQ435040    KZC14166.1    CH916366    EDV96025.1    ADTU01014853    ADTU01014854    ADTU01014855    CM000363    EDX09915.1    GL440813    EFN65390.1    NWSH01002108    PCG69156.1    GBYB01000952    JAG70719.1    GECZ01023204    GECZ01017909    JAS46565.1    JAS51860.1    PYGN01001292    PSN35949.1    GDIP01233722    JAI89679.1    ACPB03013302    ACPB03013303    GDIP01186289    LRGB01000966    JAJ37113.1    KZS14351.1    GDIQ01072105    JAN22632.1    GDIQ01021143    JAN73594.1    LJIJ01000099    ODN02678.1    GDIP01158300    JAJ65102.1    ABQF01000245    AAQR03004370    KE161334    EPQ03196.1    AAPE02018787    CU929220    CABD030047645    QRBI01000106    RMC13045.1    JP018479    AES07077.1    AEYP01006614    AGTO01010134    AACZ04054384    NBAG03000210    PNI87412.1    AGTP01108682    AGTP01108683    LSMT01000400    PFX18637.1    AAEX03000411    BC082029    JH000466    EGW03258.1    KB031156    ELK00695.1    GAMT01007850    GAMS01011247    GAMR01010674    GAMQ01006619    GAMP01010565    JAB04011.1    JAB11889.1    JAB23258.1    JAB35232.1    JAB42190.1    AANG04000169    GFFV01002612    JAV37333.1    AAGW02029086    AJFE02097215    AK301365    AL732431    CH471051    BC018999    Y08136    MUZQ01000053    OWK60754.1    JW869473    AFP01991.1    AGCU01165217    AGCU01165218    CM001279    EHH53418.1    CM001256    EHH18726.1    ADFV01165351    AQIA01049663    JSUE03032307    JSUE03032308    AEMK02000001    DQIR01191835    HDB47312.1    JH880591    ELR60418.1    RAZU01000079    RLQ74918.1   

Proteomes

UP000053268    UP000007151    UP000053240    UP000283053    UP000007266    UP000005204    + More
UP000192223    UP000027135    UP000005203    UP000242457    UP000053097    UP000279307    UP000007798    UP000008711    UP000000803    UP000001819    UP000007801    UP000053105    UP000078541    UP000008792    UP000009046    UP000078542    UP000192221    UP000008820    UP000075809    UP000007755    UP000078540    UP000008744    UP000078492    UP000076502    UP000001070    UP000005205    UP000000304    UP000000311    UP000218220    UP000245037    UP000015103    UP000076858    UP000094527    UP000007754    UP000005225    UP000189706    UP000085678    UP000001074    UP000000437    UP000001519    UP000269221    UP000000715    UP000233020    UP000248482    UP000016665    UP000007646    UP000002277    UP000005215    UP000225706    UP000002254    UP000002494    UP000233040    UP000001075    UP000010552    UP000008225    UP000011712    UP000001811    UP000240080    UP000005640    UP000197619    UP000245340    UP000007267    UP000233120    UP000009130    UP000001073    UP000233100    UP000006718    UP000008227    UP000273346   

PRIDE

H9JEU8    Q8IQD3    J9NYQ3    Q641Z7    G3HKL1    Q92484    + More
F1S2T7   

Pfam

PF00149   Metallophos

Interpro

IPR004843   Calcineurin-like_PHP_ApaH        + More
IPR029052   Metallo-depent_PP-like       
IPR042283   GpdQ_catalytic       
IPR042281   GpdQ_beta-strand       
IPR041805   ASMase/PPN1_MPP       
IPR017064   ASM-like_Pdiesterase_prd       

Gene 3D

3.60.21.10     3.30.750.180     3.60.21.40    

CDD

cd00842  MPP_ASMase

ProteinModelPortal

A0A194QIT8   A0A212ES85   A0A2H1VY23   A0A0N1IQ10   A0A2W1BKX4   A0A2H1W9F8   + More
A0A194QR38   A0A194QCX4   A0A3S2N4T6   A0A139WAW8   A0A212EIY4   H9JEU8   A0A1W4X5I9   A0A067RJ29   A0A2S2QMJ5   A0A088AER8   A0A2H8U085   A0A2A3EAU8   A0A026VYW8   B4N490   B3NGM2   Q8IQD3   Q2LZA0   B3MA63   A0A0M9A977   A0A195F186   A0A0Q9WI47   E0VZL8   A0A151IQE7   A0A1W4U962   Q172E7   A0A151XBZ8   F4X6F8   B4LG96   A0A195B2Q9   B4HAE2   A0A195E6T8   A0A154PQH5   B4J2N4   A0A158NGE5   B4QNP8   E2AMG6   A0A2A4JBL4   A0A0C9QK20   A0A1B6FNW2   A0A2P8XVA8   A0A0P4Y4T5   T1HWJ8   A0A0N8A3F6   A0A0P6DYQ7   A0A0P6HED9   A0A1D2NBQ4   A0A0P5DS43   H0ZNK5   H0XBA3   S7MGZ7   A0A1U7Q910   A0A1S3JCI9   G1PCL0   F8W4E2   G3S0A8   A0A3M0KJ37   G9KQ98   M3YSQ7   A0A2K5E582   A0A2Y9JEP0   U3KA47   G3T666   H2QTP0   I3MMQ9   A0A2B4RQL5   J9NYQ3   Q641Z7   A0A2K5PXL6   G3HKL1   L5JMX2   F7AAZ3   M3WFD8   A0A250Y163   G1SFK1   A0A287D4D5   A0A2R8ZKG5   Q92484   A0A218V436   A0A2U3W670   V9KTZ9   K7GCP6   A0A2K6CAM2   G7P391   G7MQL5   G1RU96   A0A2K5VG13   F6TB74   F1S2T7   L8IWP6   A0A3L7I9Q5  

PDB

5EBE     E-value=1.95568e-55,     Score=547

KEGG

101737031    K01128 sphingomyelin phosphodiesterase acid-like 3 [EC:3.1.4.-] | (RefSeq) acid sphingomyelinase-lik

GO

GO:0016787   GO:0016021   GO:0005615   GO:0008081   GO:0009143   GO:0004767   GO:0006685   GO:0008270   GO:0046872   GO:0005576   GO:0070062   GO:0005886   GO:0034122   GO:0050728  

Subcellular location

Secreted  

SignalP

Position:   1 - 21,         Likelihood:  0.971084

 

 

Length:

451

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

3.49276

Exp number, first 60 AAs:

1.65183

Total prob of N-in:

0.07582

outside

1  -  451

 

 

Pi

253.99837

Theta

177.433323

Tajima's D

1.424469

CLR

0.109819

CSRT

0.770011499425029

Interpretation

Uncertain