SGID Silkworm Genome Informatics Database
Gene
KWMTBOMO05270  Validated by peptides from experiments
Pre Gene Modal
BGIBMGA002413
Annotation
Contactin_[Operophtera_brumata]
Full name
Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial       + More
Coronin      
Contactin      
Alternative Name
GTP-specific succinyl-CoA synthetase subunit beta
Succinyl-CoA synthetase beta-G chain
Location in the cell
Extracellular   Reliability : 1.119
 

Sequence

CDS
ATGAAGTGTACGTTCCTTGTACTCGCAGTTGCGAGTTTGGTGAGGACCCAAGCACCTGAAAATGGTATCTATAATCAGAACTATCCGGAAGGAAACCAGAATAATACACAATATCGTCCCGGGCCCCAAATCCCTTACGGCAGTTTTCCCGTAAATAATGAATATCAACAGCCACCTCAAAGCTCTTTCTACCAGGACAGCCTCTACAACAGTGACAGCTATGCTGAAAATGAAAATGATGTCGAAAATACTGCGAGTGTATATGCTGGCTTAGATTACGAAGAGAAGTACCGATGTCCACCCAACTGGCTCCGCTACAAAGAGTCATGTTATCGGTTTACGAAGTCTCCTGATCGTCCTAGAAATGAAGCAAGAAAAATATGCCAAGCCTATGAAGCTGATTTAGTAGCCGTCAATAGCCCTGAAGAACATGGTTTTATAATAAACAATTTAATGAGGCTCGACCCTCAGAAGGGTTCATGGTATATAGGAGCTCACCAACAGTCTCCAGGTTATTGGTCCAATGATTATGATGGTTCACAGTTGACAGGTCTTGAGAATGCTTTCTTCGACGATCGACAATTTTCATTAAATAGTTATAATATATTTCCTAAAGATTTTTTGGTATACAAATTTTCACATCAAGATGGACGCTGGGGCTTAGCTGCTGTAGAAGGTACACAATATTATCACTTTATATGTGAGGGGCAAATGCAGCGTTTACATTATTTAATAGAAGAAGAAAGATCATTCACTTATGGCTTAGATACTTATGATCCAGAAAGGATTCCGAGAGGTCCATATTTTGTAAAACAGCCGGAAGATACTGTTTATGATCCTTTAAGAAATCATTATGTCCAACTTACTTGTATAGCAGGAGGATTTCCAGCTCCAACTTACAAATGGTTCGGAGAAGATTATCAAGTTGACAGTCCAGTTTACACAGAAATAGATCCTTTAGCTGACAGTAGGATTATATTAAGTGGAGGAACTTTAATGATTTATAACCCTGACAGTGAAAAGGATAATACAAAATATCACTGTACAGCTACAAACAAGTTTGGTACGATTAGATCAGAATCTGTCCGGTTGTCATTTGGATTCATAAGAGATTTCAATCAAAAGAGGCCAGTAGAAAGTGGAAATCAATACTGGGGTAAAGTAATGTATTGTGATCCTCCTTCGTACTATCCAGCTGTAAATATTCTGTGGGCTCGGAATTATTTCCCTAACCTTGTGGAAGAAGATAAACGTGTCTTTGTGTCATATGACGGTGGCCTCTATTTCTCAGCTTTAGAAACAATAGACCGAGGCAACTATAGTTGTAATGTAATGAGTAAAGTGTCTGATGAAGGCAGAAATGGTCCATTCTTTCCCTTACAGGTTTATCCGCATTCAGATTATCAGCAATTGAAATTCCCAAATAGCTTTCCTAAAGTATTTCCTGAAGCTCCAGTGGTTGGGCAAGAAGTCCGCCTGGAATGTGTCACATTTGGCTACCCAGTACCTTCTTATAATTGGACCAGACAGAATGGCCTTATACCTAAAAAAGCTCAATTGAGTAATTTTGACCGGGTTTTAACAATACCCAATGTAGGTGTAGAGGATGAAGGCGAATATATTTGTGATGTTCACAATGACAGAGCGAGGATATCAAATAGTGTGTATTTAAAAGTACAAGCTGAACCAAATTTTACAATTCCTCTTGGCGATAAACATATGGACAACAAAGGAGAATTACATTGGAACTGTGAGGCATTTGGGATACCAGATGTTAATTACACATGGTGGAAAAATGGTAAAAAACTAAAAAAAGATAATCTAGAACCACAGGACCGTGATAGGTACATTATTCAAGACAATGTTTTGATTATAAAATATTTAGACCCAACAAGAGATCCTGGAATGTACCAGTGTGAAGCAAAGAATCAATTGAAAGCTAGTTACTCGTCAGCGCAATTACGAGTGCTCTCTTTGAAACCATCATTTAAAAAACATCCTTTAGAATTGGAGACATACGGTTCTGAAGGTGGAAATGTGACATTAAAATGCAAACCAGAAGCTGCCCCAAAGCCAACATTCACTTGGAAAAAAGACAACATAGTTATTGGGGCAGGAGGAAAAAGATTCATTACCGAAAATGGGAATTTAATTATTAGACAACTTTCCAGAGATGATGAGGGCGTGTACACATGCGTCGCAAAGAATCAATACGGCACTGATGAGAGTAGAGGTCGTCTAATTGTCTTACGTGCACCACGTTTCCTTGAAAAACTCCCCCCTCGGATCACTGCACAAGTGAATTCAGTAGTTTTCTTGCACTGTAATGCCGATGTAGATCCTATTTTAGATACAGCGTATCTATGGAACCATAATGGCTTGCGGATTAAAGACTCATCAGATCTGTATGTCAACAAAAGAATAAAAATTGACGGTGGTGAGCTAACTATTTACAATGTAAGTCTTTCGGATGTGGGAGATTACGAATGTGTCGTTAAGTCCGCTATCGGAAGAATCTCTTCTCATGTGCAGCTGCGTATAGAGGGCCCGCCAGGTCCGCCGGGAGGCGTACAAATACTTGGCATACAGAGGTCTTCGGTGACCCTAGAGTGGACCGACAGTAACTCAAACGGTCGCCCTATAACTGGATACATGCTGACCGGACGCACCCACTGGAACTCAACTTGGTACGTGATCAGCGAGAATATACCCAACGTAATGGAAGTCGATAGATACAATGGGCGTAAACGAGCCACTGTTTCCACGACGCTTCTACCGTGGGCCATATATGAGTTTAGAATTCAAGCTATTAATATACTGGGTCCGGGGCAACCGTCCGCGCCGAGTCCGCAGTTCTCAACGTCGGGAGACAAGCCCTATGAGGCGCCCGCGAACGTTAGCGGCGGCGGCGGGAAGACCGGTGACCTCACAATCACGTGGACGCCCTTACCGACCTCCCTCCAAAACGGACCCGGCATACATTACAAAATATTCTGGCGCCGGAACGGCTCCGAAGTCGAATTTCAGTCCTTGCTTCTTAAGAAGTACGGAAACATCGGGACATACGTCGTGCACATATCTTCCACGTATTTCTACACGCCGTACGATGTCAAAGTACAAGCCTTCAACGATATAGGTCCCGGGCCAGAGAGCGAAGTAGTAACCATTTATTCGGCGGAGGACATGCCGCAGGTCGCGCCGCAGCTGGTCTCCGCGCGGTCCTTTAACTCCACAGCCCTGAACGTGACCTGGAACCCGATCGATCAGTCGCGCGAGAGGCTGCGCGGCAAGCTGATAGGCCACCGTCTCAAGTACTGGAAACAGGAGAACAAAGAAGAGGAGTGCATCTACTATTTGTCTCGAACGACTCGAAATTGGGCTCTTATAGTTGGTCTCCAGCCAGATACATATTATTATGTGAAGGTATAA
Protein
MKCTFLVLAVASLVRTQAPENGIYNQNYPEGNQNNTQYRPGPQIPYGSFPVNNEYQQPPQSSFYQDSLYNSDSYAENENDVENTASVYAGLDYEEKYRCPPNWLRYKESCYRFTKSPDRPRNEARKICQAYEADLVAVNSPEEHGFIINNLMRLDPQKGSWYIGAHQQSPGYWSNDYDGSQLTGLENAFFDDRQFSLNSYNIFPKDFLVYKFSHQDGRWGLAAVEGTQYYHFICEGQMQRLHYLIEEERSFTYGLDTYDPERIPRGPYFVKQPEDTVYDPLRNHYVQLTCIAGGFPAPTYKWFGEDYQVDSPVYTEIDPLADSRIILSGGTLMIYNPDSEKDNTKYHCTATNKFGTIRSESVRLSFGFIRDFNQKRPVESGNQYWGKVMYCDPPSYYPAVNILWARNYFPNLVEEDKRVFVSYDGGLYFSALETIDRGNYSCNVMSKVSDEGRNGPFFPLQVYPHSDYQQLKFPNSFPKVFPEAPVVGQEVRLECVTFGYPVPSYNWTRQNGLIPKKAQLSNFDRVLTIPNVGVEDEGEYICDVHNDRARISNSVYLKVQAEPNFTIPLGDKHMDNKGELHWNCEAFGIPDVNYTWWKNGKKLKKDNLEPQDRDRYIIQDNVLIIKYLDPTRDPGMYQCEAKNQLKASYSSAQLRVLSLKPSFKKHPLELETYGSEGGNVTLKCKPEAAPKPTFTWKKDNIVIGAGGKRFITENGNLIIRQLSRDDEGVYTCVAKNQYGTDESRGRLIVLRAPRFLEKLPPRITAQVNSVVFLHCNADVDPILDTAYLWNHNGLRIKDSSDLYVNKRIKIDGGELTIYNVSLSDVGDYECVVKSAIGRISSHVQLRIEGPPGPPGGVQILGIQRSSVTLEWTDSNSNGRPITGYMLTGRTHWNSTWYVISENIPNVMEVDRYNGRKRATVSTTLLPWAIYEFRIQAINILGPGQPSAPSPQFSTSGDKPYEAPANVSGGGGKTGDLTITWTPLPTSLQNGPGIHYKIFWRRNGSEVEFQSLLLKKYGNIGTYVVHISSTYFYTPYDVKVQAFNDIGPGPESEVVTIYSAEDMPQVAPQLVSARSFNSTALNVTWNPIDQSRERLRGKLIGHRLKYWKQENKEEECIYYLSRTTRNWALIVGLQPDTYYYVKV

Summary

Description
GTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.
Required for organization of septate junctions and paracellular barrier functions. Septate junctions, which are the equivalent of vertebrates tight junctions, are characterized by regular arrays of transverse structures that span the intermembrane space and form a physical barrier to diffusion.
Catalytic Activity
CoA + GTP + succinate = GDP + phosphate + succinyl-CoA
Cofactor
Mg(2+)
Subunit
Heterodimer of an alpha and a beta subunit. The beta subunit determines specificity for GTP.
Forms a complex with Nrg and Nrx.
Similarity
Belongs to the succinate/malate CoA ligase beta subunit family. GTP-specific subunit beta subfamily.
Belongs to the WD repeat coronin family.
Belongs to the immunoglobulin superfamily. Contactin family.
Keywords
Cell adhesion   Cell junction   Cell membrane   Complete proteome   Disulfide bond   Glycoprotein   GPI-anchor   Immunoglobulin domain   Lipoprotein   Membrane   Reference proteome   Repeat   Signal  
Feature
chain  Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial
propeptide  Removed in mature form
EC Number
6.2.1.4
EMBL
ODYU01004126    SOQ43672.1    JTDY01000914    KOB75456.1    NWSH01005469    PCG64178.1    + More
AB264690    BAG30765.1    KQ459220    KPJ02908.1    RSAL01000222    RVE44025.1    KU840799    AOF39986.1    GECZ01030373    JAS39396.1    NEVH01013208    PNF29807.1    GEDC01022085    JAS15213.1    KK852812    KDR15864.1    GBHO01043325    GBHO01043324    GBHO01043323    GBHO01043322    GBHO01043321    GBHO01043320    GBHO01043319    GBHO01043318    GBHO01043317    GBHO01026697    GBHO01026696    GDHC01011104    GDHC01009280    JAG00279.1    JAG00280.1    JAG00281.1    JAG00282.1    JAG00283.1    JAG00284.1    JAG00285.1    JAG00286.1    JAG00287.1    JAG16907.1    JAG16908.1    JAQ07525.1    JAQ09349.1    GBRD01007631    JAG58190.1    GEZM01037123    JAV82156.1    GEZM01037124    JAV82155.1    KQ981965    KYN31788.1    KK107182    QOIP01000003    EZA55992.1    RLU25075.1    KQ976691    KYM77981.1    KQ435031    KZC14024.1    ADTU01023676    KQ977574    KYN01826.1    KQ979608    KYN20411.1    GL888243    EGI64003.1    KZ288262    PBC30356.1    NNAY01001615    OXU23418.1    KQ982944    KYQ48953.1    DS235169    EEB12798.1    GFTR01008680    JAW07746.1    APGK01047078    KB741077    KB632384    ENN74113.1    ERL94243.1    GL447043    EFN86967.1    GBYB01000424    GBYB01002675    JAG70191.1    JAG72442.1    GGMS01003433    MBY72636.1    GFXV01005738    MBW17543.1    GGMR01009836    MBY22455.1    ABLF02031408    KQ971307    KYB29620.1    KQ435711    KOX79460.1    KYB29619.1    KQ767242    OAD53473.1    GANO01001293    JAB58578.1    UFQS01000048    UFQT01000048    SSW98581.1    SSX18967.1    GAKP01011867    GAKP01011863    JAC47085.1    JRES01001482    KNC22536.1    LJIJ01000398    ODM97899.1    GAMC01011748    JAB94807.1    BT083421    ACQ91625.1    CH480821    EDW54823.1    GBXI01010562    JAD03730.1    AY229991    AE014297    BT021333    AY095040    CH964272    EDW83720.1    KRF99863.1    CVRI01000004    CRK87512.1    GDIQ01211082    GDIQ01169650    GDIQ01151413    GDIQ01120620    GDIQ01064939    GDIQ01064938    GDIQ01061732    GDIQ01030315    JAL31106.1    GDIQ01064940    GDIQ01047604    JAN29797.1    GDIQ01157452    JAK94273.1    CH933806    EDW16586.1    GDIQ01157451    JAK94274.1    GDIQ01038407    JAN56330.1    GDIQ01038408    JAN56329.1    GDIQ01211084    GDIQ01151362    GDIQ01049110    JAN45627.1    GDIQ01092632    JAL59094.1    GDIQ01138361    JAL13365.1    GDIQ01107968    JAL43758.1    GDIQ01154253    JAK97472.1    GDIQ01192945    JAK58780.1    GDIQ01105629    JAL46097.1    GDIQ01090178    JAN04559.1    GDIP01213910    JAJ09492.1    OUUW01000007    SPP83581.1    GDIQ01154251    JAK97474.1    CH477414    EAT41415.1    CM000160    EDW95649.1    SPP83583.1    GDIP01225061    JAI98340.1    CH902617    EDV42866.1    KPU79964.1    GDIP01097684    JAM06031.1    JXJN01007739    GFDL01002192    JAV32853.1    GFDL01002190    JAV32855.1    CH940650    EDW67645.2    CP012526    ALC46897.1    KRF83380.1    GDIQ01053596    JAN41141.1    JXUM01025782    KQ560733    KXJ81095.1    GDIQ01015848    JAN78889.1    CM000070    EAL28758.1    CH479182    EDW34649.1    GDIP01015021    JAM88694.1    CH916377    EDV90626.1    JXUM01028746    JXUM01028747    KQ560831    KXJ80691.1   
Pfam
PF12796   Ank_2        + More
PF00041   fn3
PF07679   I-set
PF05473   UL45
PF00171   Aldedh
PF00549   Ligase_CoA
PF08442   ATP-grasp_2
PF12894   ANAPC4_WD40
PF08953   DUF1899
PF00400   WD40
PF00059   Lectin_C
PF13895   Ig_2
PF00047   ig
Interpro
IPR036770   Ankyrin_rpt-contain_sf        + More
IPR013098   Ig_I-set       
IPR013783   Ig-like_fold       
IPR003599   Ig_sub       
IPR020683   Ankyrin_rpt-contain_dom       
IPR016187   CTDL_fold       
IPR036179   Ig-like_dom_sf       
IPR003598   Ig_sub2       
IPR036116   FN3_sf       
IPR001304   C-type_lectin-like       
IPR002110   Ankyrin_rpt       
IPR003961   FN3_dom       
IPR016186   C-type_lectin-like/link_sf       
IPR007110   Ig-like_dom       
IPR008646   Herpes_UL45-like       
IPR016162   Ald_DH_N       
IPR010061   MeMal-semiAld_DH       
IPR015590   Aldehyde_DH_dom       
IPR016163   Ald_DH_C       
IPR016160   Ald_DH_CS_CYS       
IPR016161   Ald_DH/histidinol_DH       
IPR034722   Succ_CoA_betaG_euk       
IPR013815   ATP_grasp_subdomain_1       
IPR017866   Succ-CoA_synthase_bsu_CS       
IPR005809   Succ_CoA_synthase_bsu       
IPR013650   ATP-grasp_succ-CoA_synth-type       
IPR005811   CoA_ligase       
IPR016102   Succinyl-CoA_synth-like       
IPR003529   Hematopoietin_rcpt_Gp130_CS       
IPR017986   WD40_repeat_dom       
IPR024977   Apc4_WD40_dom       
IPR001680   WD40_repeat       
IPR015943   WD40/YVTN_repeat-like_dom_sf       
IPR015048   DUF1899       
IPR019775   WD40_repeat_CS       
IPR036322   WD40_repeat_dom_sf       
IPR027331   CORO7       
IPR015505   Coronin       
IPR013151   Immunoglobulin       
SUPFAM
SSF48403   SSF48403        + More
SSF48726   SSF48726       
SSF56436   SSF56436       
SSF49265   SSF49265       
SSF53720   SSF53720       
SSF52210   SSF52210       
SSF50978   SSF50978       
PDB
5I99     E-value=3.47949e-59,     Score=583

Ontologies

Topology

Subcellular location
Mitochondrion  
Cell membrane  
Cell junction  
Septate junction  
SignalP
Position:   1 - 16,         Likelihood:  0.994938
 
 
Length:
1142
Number of predicted TMHs:
0
Exp number of AAs in TMHs:
0.01393
Exp number, first 60 AAs:
0.00134
Total prob of N-in:
0.00008
outside
1  -  1142
 
 

Population Genetic Test Statistics

Pi
202.330612
Theta
183.265772
Tajima's D
0.270979
CLR
0.087149
CSRT
0.451127443627819
Interpretation
Uncertain
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