Gene

KWMTBOMO05214

Pre Gene Modal

BGIBMGA002431

Annotation

PREDICTED:_thiamin_pyrophosphokinase_1_[Bombyx_mori]

Location

Bomo_Chr9(-):11255321-11259293      View in EpiBrowser

Full name

Thiamine pyrophosphokinase       + More
Thiamin pyrophosphokinase 1      

Alternative Name

Thiamine pyrophosphokinase 1

Location in the cell

Cytoplasmic   Reliability : 2.706

CDS

ATGCACAATAAACCTAGTTTAAGATATGCAGTTCTCATTTTAAATAGACCAATATCACAAGAAGAAGCATTTATGAAAAACTTTTGGGAAAAAGCCACATTAAGAATAACAGTAGATGGTGGAGCATTAAGATGGGACCAGTTTCTCACCAGACTACCTGAGGAAATTAGTAATAAAATGAAAATACCAGATTTGATTACTGGTGATTTTGATTCAATAACAGAAGAAACTCTCCAAAAATATAAAAAGAAAGGTTGTAAGGTAGTGCACACCCCAGATCAAGATTATACAGATTTTACAAAAGCACTGAAAGAGTTGAATAATCACTGTCAAGAACATACAATTGAGGTGGACAATGTAATAGCAATAGGACAGGCTTCAGGCAGACTAGACCAAATCTTAGGAAATATACAAACTTTATATCTAGCTGAAAAACAACATTTACTTCATCCTGAAACTAAACTCTACCTAATGTCCGGTGATTCATTATCTTGGCTATTGGAACCTGGCAATCATATTATAAGCATTCCCGAGGAAACAAGGAAGCATAACAGAGCTTGGTGTTCATTAGTGCCTATTGGAGAAACTTGCCACAGTGTCACAACAAGTGGATTGATGTGGAATTTAGACAATCAAAGCTTGAAATTTGGAGAACTAGTCAGCACGTCGAATGCATTCGATGGTTCAGACACAGTCAAGATCAAATGTAGCCACACAATTCTTTGGTCTATGAAGGTGCCCAGTTTGACAGGAGAATAA

Protein

MHNKPSLRYAVLILNRPISQEEAFMKNFWEKATLRITVDGGALRWDQFLTRLPEEISNKMKIPDLITGDFDSITEETLQKYKKKGCKVVHTPDQDYTDFTKALKELNNHCQEHTIEVDNVIAIGQASGRLDQILGNIQTLYLAEKQHLLHPETKLYLMSGDSLSWLLEPGNHIISIPEETRKHNRAWCSLVPIGETCHSVTTSGLMWNLDNQSLKFGELVSTSNAFDGSDTVKIKCSHTILWSMKVPSLTGE

Description

Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. Can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate.

Catalytic Activity

ATP + thiamine = AMP + H(+) + thiamine diphosphate

Subunit

Homodimer.

Similarity

Belongs to the thiamine pyrophosphokinase family.
Belongs to the enoyl-CoA hydratase/isomerase family.

Keywords

3D-structure   Alternative splicing   ATP-binding   Complete proteome   Kinase   Nucleotide-binding   Reference proteome   Transferase  

Feature

chain  Thiamin pyrophosphokinase 1
splice variant  In isoform 3.

Uniprot

A0A194QHE2   A0A2H1VMH9   A0A212ESN4   A0A3S2LCP0   A0A2A4J547   A0A194RNJ0   + More
V5I9I0   A0A1B0CAN8   B0X7F9   A0A1Q3FJT3   A0A1Y1JTR1   A0A182FNN9   A0A2M4AHT2   W5JJV2   A0A2M4AH18   A0A2M4AGZ1   A0A2M4AHY5   A0A2M4BWQ1   T1E9W7   A0A067RJS6   A0A2M4BVU1   A0A2M4BWX1   T1GTI5   A0A2M4BXC7   J3JUK3   A0A131XFB5   A0A1Y9HFB6   A0A2M3ZC20   C1BVE9   D3PJQ4   A0A023FP32   A0A182JUP7   U5EJ18   Q175Q4   A0A0K8TMN4   A0A182MFE1   A0A224Z5K2   L7LZG3   A0A023GBB3   A0A131Z148   A0A023ELM5   G3MR72   A0A2R5L890   A0A2K8JX53   C1BTH3   A0A182UUV8   A0A0K8R8Y1   A0A1Y9IVZ1   A0A182WRQ4   A0A182U6J6   A0A1A9WJK7   A0A182HU24   A0A182LG03   D6W952   A0A0L0CS03   Q7QD56   A0A139WMT9   A0A182P6N6   K7J2E2   A0A2P8XMV1   A0A131XYR3   A0A310SEX6   A0A154PNU9   A0A1B0AAB9   A0A1A9UN97   A0A182XWD1   A0A1I8Q7U0   Q28HS1   A0A1B0G6V7   F6TP13   A0A084VZB1   A0A232ENS3   A0A293MYH5   A0A240PNY2   A0A182SMJ9   A0A151WZI5   F4W4E8   A0A1B6DCN3   G3HW81   A0A1W4X2H6   A0A0L7QWN9   A0A182QE81   A0A1L8FWN4   A0A0T6BC94   G3QVB4   W5QAX1   F7EK85   Q9R0M5   A0A1Z5L5K6   A0A0C9RUM1   E2BB74   A0A2U3WL60   A0A2K5VA75   E9JC14   A0A2I2ZCZ0   A0A286X7B8   I3N387   G1P1W6  

EC Number

2.7.6.2

Pubmed

26354079    22118469    28004739    20920257    23761445    24845553    + More
22516182    23537049    28049606    17510324    26369729    28797301    25576852    26830274    24945155    22216098    20966253    18362917    19820115    26108605    12364791    14747013    17210077    20075255    29403074    25244985    20431018    24438588    28648823    21719571    21804562    27762356    22398555    20809919    18464734    10567383    16141072    15489334    21183079    11419946    16365036    28528879    20798317    21282665    21993624   

EMBL

KQ459220    KPJ02861.1    ODYU01003368    SOQ42039.1    AGBW02012778    OWR44451.1    + More
RSAL01000231    RVE43845.1    NWSH01002966    PCG67205.1    KQ459984    KPJ18979.1    GALX01002900    JAB65566.1    AJWK01004132    DS232451    EDS41909.1    GFDL01007208    JAV27837.1    GEZM01101277    JAV52704.1    GGFK01006986    MBW40307.1    ADMH02001019    ETN64391.1    GGFK01006759    MBW40080.1    GGFK01006723    MBW40044.1    GGFK01007069    MBW40390.1    GGFJ01008220    MBW57361.1    GAMD01000937    JAB00654.1    KK852470    KDR23258.1    GGFJ01008002    MBW57143.1    GGFJ01008392    MBW57533.1    CAQQ02110234    CAQQ02110235    GGFJ01008237    MBW57378.1    APGK01017098    BT126918    KB739995    KB630277    KB632201    AEE61880.1    ENN82044.1    ERL83504.1    ERL90053.1    GEFH01002387    JAP66194.1    GGFM01005356    MBW26107.1    BT078578    ACO13002.1    BT121860    ADD38790.1    GBBK01001453    JAC23029.1    GANO01002406    JAB57465.1    CH477396    EAT41838.1    GDAI01002182    JAI15421.1    AXCM01004068    GFPF01012313    MAA23459.1    GACK01008795    JAA56239.1    GBBM01004216    JAC31202.1    GEDV01004077    JAP84480.1    GAPW01003440    JAC10158.1    JO844373    AEO35990.1    GGLE01001587    MBY05713.1    KY031246    ATU82997.1    BT077902    ACO12326.1    GADI01006874    JAA66934.1    APCN01000602    KQ971312    EEZ98443.2    JRES01000091    KNC34204.1    AAAB01008859    EAA07620.4    KYB29167.1    AAZX01007551    PYGN01001697    PSN33329.1    GEFM01003423    JAP72373.1    KQ780345    OAD51950.1    KQ434984    KZC13134.1    CR760766    CAJ83166.1    CCAG010013874    AAMC01097512    AAMC01097513    AAMC01097514    AAMC01097515    AAMC01097516    AAMC01097517    AAMC01097518    AAMC01097519    AAMC01097520    AAMC01097521    ATLV01018673    KE525245    KFB43305.1    NNAY01003086    OXU20005.1    GFWV01020818    MAA45546.1    KQ982649    KYQ53111.1    GL887532    EGI70843.1    GEDC01013845    JAS23453.1    JH000818    EGW12555.1    KQ414710    KOC62986.1    AXCN02001586    CM004476    OCT75986.1    LJIG01001956    KRT84984.1    CABD030055672    CABD030055673    CABD030055674    CABD030055675    CABD030055676    CABD030055677    CABD030055678    CABD030055679    CABD030055680    CABD030055681    AMGL01089547    AMGL01089548    AMGL01089549    AMGL01089550    AMGL01089551    AMGL01089552    AMGL01089553    AMGL01089554    AMGL01089555    AB027568    AK028431    AK035044    AK039131    AK136486    BC015246    BC023354    GFJQ02004274    JAW02696.1    GBYB01012555    GBYB01012558    JAG82322.1    JAG82325.1    GL446946    EFN87047.1    AQIA01048568    AQIA01048569    AQIA01048570    AQIA01048571    AQIA01048572    AQIA01048573    AQIA01048574    GL771642    EFZ09677.1    AAKN02015382    AAKN02015383    AAKN02015384    AAKN02015385    AAKN02015386    AAKN02015387    AGTP01044987    AGTP01044988    AGTP01044989    AGTP01044990    AGTP01044991    AGTP01044992    AGTP01044993    AGTP01044994    AGTP01044995    AGTP01044996    AAPE02029221    AAPE02029222    AAPE02029223    AAPE02029224    AAPE02029225    AAPE02029226   

Proteomes

UP000053268    UP000007151    UP000283053    UP000218220    UP000053240    UP000092461    + More
UP000002320    UP000069272    UP000000673    UP000027135    UP000015102    UP000019118    UP000030742    UP000075900    UP000075881    UP000008820    UP000075883    UP000075903    UP000075920    UP000076407    UP000075902    UP000091820    UP000075840    UP000075882    UP000007266    UP000037069    UP000007062    UP000075885    UP000002358    UP000245037    UP000076502    UP000092445    UP000078200    UP000076408    UP000095300    UP000092444    UP000008143    UP000030765    UP000215335    UP000075880    UP000075901    UP000075809    UP000007755    UP000001075    UP000192223    UP000053825    UP000075886    UP000186698    UP000001519    UP000002356    UP000002279    UP000000589    UP000008237    UP000245340    UP000233100    UP000005447    UP000005215    UP000001074   

PRIDE

Q9R0M5   

Pfam

PF04265   TPK_B1_binding        + More
PF04263   TPK_catalytic
PF00378   ECH_1
PF01336   tRNA_anti-codon
PF00152   tRNA-synt_2

Interpro

IPR006282   Thi_PPkinase        + More
IPR036759   TPK_catalytic_sf       
IPR007373   Thiamin_PyroPKinase_B1-bd       
IPR036371   TPK_B1-bd_sf       
IPR007371   TPK_catalytic       
IPR016966   Thiamin_pyrophosphokinase_euk       
IPR018376   Enoyl-CoA_hyd/isom_CS       
IPR014748   Enoyl-CoA_hydra_C       
IPR001753   Enoyl-CoA_hydra/iso       
IPR029045   ClpP/crotonase-like_dom_sf       
IPR004364   aa-tRNA-synt_II       
IPR004365   NA-bd_OB_tRNA       
IPR012340   NA-bd_OB-fold       
IPR004522   Asn-tRNA-ligase       
IPR002312   Asp/Asn-tRNA-synth_IIb       
IPR006195   aa-tRNA-synth_II       

SUPFAM

SSF63999   SSF63999        + More
SSF63862   SSF63862       
SSF52096   SSF52096       
SSF50249   SSF50249       

Gene 3D

3.40.50.10240     1.10.12.10    

CDD

cd07995  TPK

ProteinModelPortal

A0A194QHE2   A0A2H1VMH9   A0A212ESN4   A0A3S2LCP0   A0A2A4J547   A0A194RNJ0   + More
V5I9I0   A0A1B0CAN8   B0X7F9   A0A1Q3FJT3   A0A1Y1JTR1   A0A182FNN9   A0A2M4AHT2   W5JJV2   A0A2M4AH18   A0A2M4AGZ1   A0A2M4AHY5   A0A2M4BWQ1   T1E9W7   A0A067RJS6   A0A2M4BVU1   A0A2M4BWX1   T1GTI5   A0A2M4BXC7   J3JUK3   A0A131XFB5   A0A1Y9HFB6   A0A2M3ZC20   C1BVE9   D3PJQ4   A0A023FP32   A0A182JUP7   U5EJ18   Q175Q4   A0A0K8TMN4   A0A182MFE1   A0A224Z5K2   L7LZG3   A0A023GBB3   A0A131Z148   A0A023ELM5   G3MR72   A0A2R5L890   A0A2K8JX53   C1BTH3   A0A182UUV8   A0A0K8R8Y1   A0A1Y9IVZ1   A0A182WRQ4   A0A182U6J6   A0A1A9WJK7   A0A182HU24   A0A182LG03   D6W952   A0A0L0CS03   Q7QD56   A0A139WMT9   A0A182P6N6   K7J2E2   A0A2P8XMV1   A0A131XYR3   A0A310SEX6   A0A154PNU9   A0A1B0AAB9   A0A1A9UN97   A0A182XWD1   A0A1I8Q7U0   Q28HS1   A0A1B0G6V7   F6TP13   A0A084VZB1   A0A232ENS3   A0A293MYH5   A0A240PNY2   A0A182SMJ9   A0A151WZI5   F4W4E8   A0A1B6DCN3   G3HW81   A0A1W4X2H6   A0A0L7QWN9   A0A182QE81   A0A1L8FWN4   A0A0T6BC94   G3QVB4   W5QAX1   F7EK85   Q9R0M5   A0A1Z5L5K6   A0A0C9RUM1   E2BB74   A0A2U3WL60   A0A2K5VA75   E9JC14   A0A2I2ZCZ0   A0A286X7B8   I3N387   G1P1W6  

PDB

2F17     E-value=6.63104e-48,     Score=479

KEGG

101745539    K00949 thiamine pyrophosphokinase [EC:2.7.6.2] | (RefSeq) thiamin pyrophosphokinase 1

PATHWAY

00730    Thiamine metabolism - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0006772   GO:0004788   GO:0009229   GO:0016301   GO:0005524   GO:0030975   GO:0004816   GO:0006421   GO:0003676   GO:0003677   GO:0001664   GO:0005525   GO:0016491   GO:0003824  

Length:

252

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.01733

Exp number, first 60 AAs:

0.00033

Total prob of N-in:

0.07807

outside

1  -  252

 

 

Pi

356.496623

Theta

191.806993

Tajima's D

2.082312

CLR

0.129674

CSRT

0.896205189740513

Interpretation

Uncertain