SGID Silkworm Genome Informatics Database
Gene
KWMTBOMO05196  Validated by peptides from experiments
Pre Gene Modal
BGIBMGA002457
Annotation
PREDICTED:_retinal_dehydrogenase_1-like_[Amyelois_transitella]
Full name
Retinal dehydrogenase 1       + More
Aldehyde dehydrogenase, cytosolic 1      
Alternative Name
Aldehyde dehydrogenase, cytosolic
ALDH class 1
ALDH-E1
ALHDII
Aldehyde dehydrogenase family 1 member A7
Aldehyde dehydrogenase phenobarbital-inducible
Location in the cell
Cytoplasmic   Reliability : 2.667
 

Sequence

CDS
ATGGTCCCGGAAATTAAATACACCAAGTTGTTCATTGACAACGAATGGGTTGATGCGAAAGATGGCGAAACATTTGATACATTCAGTCCACACGACGGATCAGTCATCGCTAAAGTAGCCAAAGGACAAAAGGCTGATATTGACGCCGCAGTGGCAGCGGCGAAGCGTGCGTTCCAACGTAACTCAGAATGGCGGTTGGCGGATGCCTCGCAGCGCGGAAAACTACTTTACAAGTTCGCAGATCTTATTGAACGCGACTTCGAATATCTTGCGAAACTCGAGTCATATGACAATGGCATGGTTTATAAAAATTGCCTTAGAATACTTTCAGGAATTCCTGACAGTGTAAGATACCTAGGCAGTCTGGCGGACAAAATCCAAGGCGACACAATACCAACTGATGGCGATTCATTCAGTTACACATTGAAACAACCCGTTGGAGTGTGTGGTCTCATTTTACCCTGGAACGTTCCCATCCTGATGTTTCTGAACAAGGTCGTAACTGCCTTAGCAGCAGGATGTACAGTGGTAGTGAAGCCGGCAGAACAAACTCCATTAACTGCTTTGGCATTGGCCGCACTGTTGAAAGAAGCTGGCATACCTAAGGGTGTAGTCAACATCGTGCCAGGATTCGGAGAAGATGCAGGAGTGGCGCTCACACATCACCCTGATGTTGCCAAAATTTCCTTTACAGGATCTTTGGAGGTAGGCAAACTGATCCAAAAGGCAGCTGGAACAAACAATTTGAAGCGTGTCACTCTCGAACTCGGAGGCAAGAGTCCACTAATCATAATGAATGACGCCGATTTAAATTTAGCCATCCCTTATGCTGGCAGCGGTGTATTTTTCCACCAAGGACAAATTTGCGTGGCTGCTTCCCGCTTGTACGTCCAGTCGGGTATTTACGACAAATTCGTGAAGGGGGCCGTTGAATATGCAAAAGCTATCCGGGTCGGCAATCCTTTTGACGAATCAACTGTAAACGGACCACAGATTGACGAGTCTATGATGAATAAAGTTTTGGGCTTAATAGAGACCGGAAAAAAAGAAGGAGCGAACCTACTGGCAGGTGGAGAACGTATTGGTACCGCCGGATATTATATCCAGCCTACTGTGTTTGCAGATGTTAATGATGATATGACTATTGCTAAGGAAGAGATTTTCGGTCCAGTCCAGAGCATTCTAAAGTTCGATACGTTGGAAGAAGTGATCGATCGCGCCAACAATACAAATTACGGTCTTGCAGCTGGAATCTTCACTACAAACCTTAACAACGCATTGCAGTTTAGTAAGCATGTCGAGGCCGGAACAGTTTGGGTGAATAACTACCTGACAATGGCACATCAAGCACCTTTCGGAGGATTCAAGGACTCAGGAATTGGCCGTGAAAATGGTATCAAAGCAGTTGAAGCATATTTGGAAGAGAAGACTGTCATCATTTCTCTCCCTAAAAAGCTTTGA
Protein
MVPEIKYTKLFIDNEWVDAKDGETFDTFSPHDGSVIAKVAKGQKADIDAAVAAAKRAFQRNSEWRLADASQRGKLLYKFADLIERDFEYLAKLESYDNGMVYKNCLRILSGIPDSVRYLGSLADKIQGDTIPTDGDSFSYTLKQPVGVCGLILPWNVPILMFLNKVVTALAAGCTVVVKPAEQTPLTALALAALLKEAGIPKGVVNIVPGFGEDAGVALTHHPDVAKISFTGSLEVGKLIQKAAGTNNLKRVTLELGGKSPLIIMNDADLNLAIPYAGSGVFFHQGQICVAASRLYVQSGIYDKFVKGAVEYAKAIRVGNPFDESTVNGPQIDESMMNKVLGLIETGKKEGANLLAGGERIGTAGYYIQPTVFADVNDDMTIAKEEIFGPVQSILKFDTLEEVIDRANNTNYGLAAGIFTTNLNNALQFSKHVEAGTVWVNNYLTMAHQAPFGGFKDSGIGRENGIKAVEAYLEEKTVIISLPKKL

Summary

Description
Can convert/oxidize retinaldehyde to retinoic acid. Binds free retinal and cellular retinol-binding protein-bound retinal (By similarity). May have a broader specificity and oxidize other aldehydes in vivo (By similarity).
Can oxidize benzaldehyde, propionaldehyde and acetaldehyde. No detectable activity with retinal.
Catalytic Activity
all-trans-retinal + H2O + NAD(+) = all-trans-retinoate + 2 H(+) + NADH
9-cis-retinal + H2O + NAD(+) = 9-cis-retinoate + 2 H(+) + NADH
11-cis-retinal + H2O + NAD(+) = 11-cis-retinoate + 2 H(+) + NADH
an aldehyde + H2O + NAD(+) = a carboxylate + 2 H(+) + NADH
Biophysicochemical Properties
2.4 mM for acetaldehyde
1.6 mM for propionaldehyde
4.7 uM for benzaldehyde
Subunit
Homotetramer.
Similarity
Belongs to the aldehyde dehydrogenase family.
Keywords
Acetylation   Complete proteome   Cytoplasm   Direct protein sequencing   NAD   Oxidoreductase   Phosphoprotein   Reference proteome  
Feature
chain  Retinal dehydrogenase 1
EC Number
1.2.1.-
1.2.1.36
1.2.1.3
EMBL
BABH01010692    BABH01010693    BABH01010694    BABH01010695    BABH01010696    RSAL01000402    + More
RVE41911.1    KQ459597    KPI94745.1    RSAL01000037    RVE51196.1    KQ459984    KPJ18941.1    ODYU01005422    SOQ46251.1    KQ459606    KPI91415.1    KPJ18940.1    ODYU01006360    SOQ48142.1    KPI94744.1    ODYU01004072    SOQ43566.1    KPI94743.1    AGBW02008996    OWR51929.1    KPJ18956.1    JTDY01003290    KOB69791.1    NWSH01003948    PCG65640.1    PCG65639.1    NWSH01001358    PCG71550.1    DS232392    EDS41034.1    RBVL01000220    RKO10496.1    GFDL01008464    JAV26581.1    AXCN02000654    CH477625    EAT38124.1    JXUM01078869    KQ563093    KXJ74487.1    GAPW01001149    JAC12449.1    AAAB01008980    GAMD01003251    JAA98339.1    GGFK01006020    MBW39341.1    GGFK01006088    MBW39409.1    APCN01001694    GGLE01006443    MBY10569.1    ADMH02000539    ETN66060.1    KK853170    KDR10336.1    ATLV01017583    KE525174    KFB42296.1    NCKU01001310    RWS12467.1    GFAH01000487    JAV47902.1    AXCM01004965    GEIB01000036    JAR87698.1    EAA14068.3    GFWV01019295    MAA44023.1    KQ459603    KPI92727.1    ODYU01002733    SOQ40599.1    NCKU01002086    RWS10468.1    AGBW02013494    OWR43337.1    NNAY01002022    OXU22287.1    NCKU01007299    RWS02740.1    JTDY01001120    KOB74817.1    CVRI01000006    CRK88023.1    GBHO01036212    JAG07392.1    GBRD01015069    JAG50757.1    NEVH01019075    PNF22959.1    ODYU01004073    SOQ43567.1    RAZU01000133    RLQ71138.1    GFJQ02000226    JAW06744.1    AGCU01194510    AGCU01194511    AGCU01194512    AGCU01194513    AGCU01194514    AGCU01194515    AGCU01194516    AGCU01194517    AGCU01194518    MH990439    AYV88986.1    KQ460883    KPJ11229.1    KZ505748    PKU45677.1    GBSH01002235    JAG66791.1    KQ971326    EEZ97369.1    BDGG01000018    GAV08728.1    MH979760    AYV89215.1    PNF22957.1    AAKN02026169    AAKN02026170    AAKN02026171    AAKN02026172    AAKN02026173    AEFK01077107    AEFK01077108    AEFK01077109    AEFK01077110    AEFK01077111    AEFK01077112    AEFK01077113    AEFK01077114    AEFK01077115    AEFK01077116    PNF22956.1    BABH01005570    BABH01005571    BABH01005572    BABH01005573    DQ311283    ABD36228.1    GAAZ01002032    JAA95911.1    GBEX01002791    JAI11769.1    GEZM01076997    JAV63479.1    ODYU01004657    SOQ44774.1    GAPW01001148    JAC12450.1    BC076716    AAH76716.1    AB016718    BAA76412.1    GFDL01009835    JAV25210.1    GBBM01000946    JAC34472.1    BC077256    AAH77256.1    CH478186    EAT33638.1    M23995    AF061833    AAC69552.1   
Pfam
PF00171   Aldedh
Interpro
IPR016161   Ald_DH/histidinol_DH        + More
IPR016163   Ald_DH_C       
IPR016160   Ald_DH_CS_CYS       
IPR029510   Ald_DH_CS_GLU       
IPR016162   Ald_DH_N       
IPR015590   Aldehyde_DH_dom       
IPR011407   10_FTHF_DH       
SUPFAM
SSF53720   SSF53720       
Gene 3D
PDB
5L2O     E-value=7.28088e-148,     Score=1344

Ontologies

Topology

Subcellular location
  
Cytoplasm  
Length:
486
Number of predicted TMHs:
0
Exp number of AAs in TMHs:
0.26137
Exp number, first 60 AAs:
0
Total prob of N-in:
0.01364
outside
1  -  486
 
 

Population Genetic Test Statistics

Pi
171.746592
Theta
143.64057
Tajima's D
0.586666
CLR
0
CSRT
0.538823058847058
Interpretation
Uncertain

Multiple alignment of Orthologues

 
 

Gene Tree

 
 
Peptides ×
Source Sequence Identity Evalue
28556443 GVVNIVPGFGEDAGVALTHHPDVAK 100.00 5e-13
28556443 FDTLEEVIDR 100.00 9e-11
28556443 FDTLEEVIDR 100.00 9e-11
26822097 YIGSIADK 96.00 2e-10
28556443 ADIDAAVAAAK 100.00 7e-10
26822097 DFEYIAK 100.00 4e-09
28556443 PAEQTPLTALALAALLK 100.00 4e-09
28556443 PAEQTPLTALALAALLK 100.00 4e-09
28556443 FADLIER 100.00 7e-07
26822097 GVVNIVPGFGEDAGVAITHHPDVAK 100.00 2e-06
28556443 DFEYLAK 100.00 2e-06
28556443 ILSGIPDSVR 100.00 2e-06
28556443 ILSGIPDSVR 100.00 4e-06
28556443 HVEAGTVWVNNYLTMAHQAPFGGFK 100.00 4e-06
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