SGID Silkworm Genome Informatics Database
Gene
KWMTBOMO04752
Pre Gene Modal
BGIBMGA009882
Annotation
PREDICTED:_nitrogen_permease_regulator_2-like_protein_[Papilio_polytes]
Full name
GATOR complex protein NPRL2      
Alternative Name
Nitrogen permease regulator 2-like protein
Location in the cell
Extracellular   Reliability : 1.166 Nuclear   Reliability : 1.819
 

Sequence

CDS
ATGGATGGGAATATGAAATATTACGAAGGATGTGGCCAAGAAGGGCCCATTCGTTGTATATTCCTGTGTGAATTTCACCCCACCGCTGGACCCAAAATAACTTGCCAAGTGCCAGAAAATTACATTTCGAAAGATATTTTCGATACTGTCAGCCACTACATCATACCGAAGGTTCAACTTCAGCGATGCACCCTAACAGTGACCCTTCTCGGTTCGAAGATCCTGGGTTTCCCAGTGAGGATCGACAATAAGAAGTACGCCCGCAACGCGTATTATTTTAATCTTTGCTTCGTGTGCGACGCGTGGGCCAGGACTGTTCACCTTGAGCCCTTAGTCAAAAAACTCACAGAGTATCTACTATCGATGGAGTTGGAGTCAGAGTGGTTGTCGAAGCAGTCGACGTCTGGTGACGCGAAGGACCTCGGCTGTCTGATGCGGCAGGTCATGCAGGACATCAACTGCAGGAGAATGTGCACGCTGACCGTTGGTACGACAACGACCCACCTGACCGTGGTCCGGGTGAACAGCGATCCGGCTCCCGTTAAAGATCACCAGGTCCCGGTCTTCTTGTACAGCAGACGGTCTTTTGTGACGGACCAATGGGACCTCACCACTAATCAGATACTCCCCTACATTGACGGCTTCAATCACGTTTCCAAGATCGCAGCCCTGACGGACGTTGAGATCAGTCTGGTACGAGCCTGCGTCCAGAACCTGGTGTACTACGGCGTGGTGACCCTTGTGCCCATCTTCCAGTACTGCGCGGTCTACAGCGCGACGCCGAAGCTAAGACAGCTGACAAGATGCGCAGGCCTTCAGAGACAGTGCGTCGAATTCTGTGCCAGATCTCCACGGCACTTGCCCAAAGTCAGCGATATATTCCGTATGTACGCCGGAATGTCGTATGGGAGTACCATCAAGGACCTCTGCAGGAGGATGAAGCCACAGGAACTAGCTATAAACGAGAGGAAGTTGGTGCTATTCGGAGTGCTCGAGGGTCTTATACGAAGGGTTTATAAGTATCCAATAACTTTGAACGACGAGACGGCGTCGCTTCGAAGCGACCACAGCCAGTCGGTAGCGAGGACGTACAACGGGCTCGTGTGCCTGGACGAGCTGTGCTGCCAGGCGGGTCTGTCCGCGTCGCAGCTCGACGACCAACTGGAGCGGGACACGAACGTCGCCTTCATCGTCAAGTGA
Protein
MDGNMKYYEGCGQEGPIRCIFLCEFHPTAGPKITCQVPENYISKDIFDTVSHYIIPKVQLQRCTLTVTLLGSKILGFPVRIDNKKYARNAYYFNLCFVCDAWARTVHLEPLVKKLTEYLLSMELESEWLSKQSTSGDAKDLGCLMRQVMQDINCRRMCTLTVGTTTTHLTVVRVNSDPAPVKDHQVPVFLYSRRSFVTDQWDLTTNQILPYIDGFNHVSKIAALTDVEISLVRACVQNLVYYGVVTLVPIFQYCAVYSATPKLRQLTRCAGLQRQCVEFCARSPRHLPKVSDIFRMYAGMSYGSTIKDLCRRMKPQELAINERKLVLFGVLEGLIRRVYKYPITLNDETASLRSDHSQSVARTYNGLVCLDELCCQAGLSASQLDDQLERDTNVAFIVK

Summary

Description
An essential component of the GATOR subcomplex GATOR1 which functions as an inhibitor of the amino acid-sensing branch of the TORC1 signaling pathway (PubMed:23723238, PubMed:27166823, PubMed:25512509). The two GATOR subcomplexes, GATOR1 and GATOR2, regulate the TORC1 pathway in order to mediate metabolic homeostasis, female gametogenesis and the response to amino acid limitation and complete starvation (PubMed:23723238, PubMed:27166823, PubMed:25512509). The function of GATOR1 in negatively regulating the TORC1 pathway is essential for maintaining baseline levels of TORC1 activity under nutrient rich conditions, and for promoting survival during amino acid or complete starvation by inhibiting TORC1-dependent cell growth and promoting catabolic metabolism and autophagy (PubMed:23723238, PubMed:27166823). In addition, this inhibition of TORC1 is necessary to maintain female fertility under normal conditions and during periods of nutrient stress (PubMed:24786828, PubMed:27672113, PubMed:25512509). GATOR1 and GATOR2 act at different stages of oogenesis to regulate TORC1 in order to control meiotic entry and promote oocyte growth and development (PubMed:25512509). After exactly four mitotic cyst divisions, the GATOR1 complex members (Iml1, Nprl2 and Nprl3) down-regulate TORC1 to slow cellular metabolism and promote the mitotic/meiotic transition (PubMed:25512509). At later stages of oogenesis, the mio and Nup44A components of the GATOR2 complex inhibit GATOR1 and thus activate TORC1 to promote meiotic progression, and drive oocyte growth and development (PubMed:25512509).
Subunit
Component of the GATOR complex consisting of mio, Nup44A/Seh1, Im11, Nplr3, Nplr2, Wdr24, Wdr59 and Sec13 (PubMed:27166823). Within the GATOR complex, probable component of the GATOR1 subcomplex which is likely composed of Iml1, Nplr2 and Nplr3 (PubMed:27166823). Interacts with Nprl3 (PubMed:24786828).
Similarity
Belongs to the cyclin family.
Belongs to the NPR2 family.
Keywords
Cell cycle   Cell division   Complete proteome   Cytoplasm   Lysosome   Meiosis   Mitosis   Reference proteome  
Feature
chain  GATOR complex protein NPRL2
EMBL
KQ461192    KPJ07023.1    KQ459595    KPI95728.1    KK853297    KDR08783.1    + More
KQ763450    OAD55051.1    KZ288219    PBC32229.1    GL438491    EFN68896.1    GL447585    EFN86340.1    KQ435048    KZC14209.1    GEDC01019358    JAS17940.1    KQ977793    KYM99659.1    NEVH01020852    PNF21147.1    KQ982339    KYQ57322.1    KQ415041    KOC58901.1    KQ976574    KYM80279.1    ADTU01015170    GEBQ01006360    JAT33617.1    GL764074    EFZ18599.1    KQ981625    KYN39081.1    KK107135    EZA58042.1    KQ980107    KYN17649.1    GL888480    EGI60283.1    KQ435833    KOX71614.1    QOIP01000006    RLU21711.1    GECU01012639    JAS95067.1    LJIG01009518    KRT82919.1    GECL01000597    JAP05527.1    GBBI01003762    JAC14950.1    GFTR01005781    JAW10645.1    ACPB03021733    GEZM01013053    JAV92796.1    KQ971358    EFA08261.1    GBGD01001752    JAC87137.1    GBHO01015172    GDHC01006029    JAG28432.1    JAQ12600.1    APGK01056842    BT128328    KB741277    KB632028    AEE63286.1    ENN71159.1    ERL88120.1    KK852819    KDR15634.1    GL732618    EFX70671.1    GDIP01023243    JAM80472.1    GDIQ01208454    GDIQ01208453    JAK43272.1    JXUM01109210    KQ565299    KXJ71113.1    JXUM01024583    KQ560695    KXJ81235.1    CH477284    EAT44769.1    CH479067    EAT32519.1    GAMC01007492    JAB99063.1    JXJN01000553    JXJN01000554    JXJN01000555    DS234994    EEB10060.1    GANO01000142    JAB59729.1    DS234210    EDS33167.1    CM000162    EDX02542.1    CH480832    EDW46200.1    CH940651    EDW65809.1    NNAY01000761    OXU26639.1    CM000366    EDX18151.1    CH954180    EDV46435.1    GDHF01027109    JAI25205.1    AE003503    AY051914    AAK93338.1    GAKP01005748    GAKP01005747    JAC53205.1    OUUW01000003    SPP78124.1    CH379066    EAL31390.1    CH479235    EDW36710.1    GBXI01003415    JAD10877.1    CH933810    EDW06753.1    GECZ01004308    JAS65461.1    CH964239    EDW82469.1    CH902640    EDV38325.1    GFJQ02003814    JAW03156.1    CH916370    EDW00309.1    JH431551    CP012528    ALC48269.1    GDIQ01151969    JAK99756.1    LRGB01000359    KZS19577.1    GDIP01079402    JAM24313.1    AXCN02001237    GDIQ01212706    JAK39019.1    GDAI01002106    JAI15497.1    GFPF01005429    MAA16575.1    GEDV01002589    JAP85968.1    NCKU01005917    NCKU01005916    RWS04038.1    RWS04041.1    AAAB01008846    EAA06517.3    APCN01001313   
Pfam
PF06218   NPR2        + More
PF00134   Cyclin_N
PF07992   Pyr_redox_2
PF00620   RhoGAP
PF00788   RA
Interpro
IPR009348   NPR2        + More
IPR036188   FAD/NAD-bd_sf       
IPR023753   FAD/NAD-binding_dom       
IPR013763   Cyclin-like       
IPR006671   Cyclin_N       
IPR015904   Sulphide_quinone_reductase       
IPR036915   Cyclin-like_sf       
IPR008936   Rho_GTPase_activation_prot       
IPR000198   RhoGAP_dom       
IPR029071   Ubiquitin-like_domsf       
IPR001849   PH_domain       
IPR000159   RA_dom       
SUPFAM
SSF47954   SSF47954        + More
SSF51905   SSF51905       
SSF48350   SSF48350       
SSF54236   SSF54236       
Gene 3D
PDB
6CET     E-value=9.67738e-82,     Score=773

Ontologies

Topology

Subcellular location
Cytoplasm   Localizes primarily to the autolysosomes during amino-acid starvation.   With evidence from 2 publications.
Lysosome   Localizes primarily to the autolysosomes during amino-acid starvation.   With evidence from 2 publications.
Length:
399
Number of predicted TMHs:
0
Exp number of AAs in TMHs:
9.52240999999998
Exp number, first 60 AAs:
0.02274
Total prob of N-in:
0.28878
outside
1  -  399
 
 

Population Genetic Test Statistics

Pi
260.155429
Theta
156.931425
Tajima's D
1.954686
CLR
0.247586
CSRT
0.875506224688766
Interpretation
Uncertain
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