Gene

KWMTBOMO04640

Annotation

PREDICTED:_methylated-DNA--protein-cysteine_methyltransferase_isoform_X1_[Bombyx_mori]

Location

Bomo_Chr8(-):11294313-11295360      View in EpiBrowser

Full name

Probable methylated-DNA--protein-cysteine methyltransferase       + More
Methylated-DNA--protein-cysteine methyltransferase      

Alternative Name

6-O-methylguanine-DNA methyltransferase
O-6-methylguanine-DNA-alkyltransferase

Location in the cell

Mitochondrial   Reliability : 2.409

CDS

ATGAAATTACAAGAAATTCCCTTTGGCTCCACTTATACATATAGTGACATTACTAAGGCATTGGGACGACCAACAACCCATGCTAGAGCAGTAGGTTCAGCCTGTGGTGCCAATACACATCTTATAGTTACTCCATGTCATAGATTAATTAGTTTATCTTCTAAAGGTGGATTTAATAGTGGAATCAATCGTAAAGAATGGCTTATTGAACATGAGAAGAAATATGCAAACAATCTATGA

Protein

MKLQEIPFGSTYTYSDITKALGRPTTHARAVGSACGANTHLIVTPCHRLISLSSKGGFNSGINRKEWLIEHEKKYANNL

Description

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Catalytic Activity

a 6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] = a 2'-deoxyguanosine in DNA + S-methyl-L-cysteinyl-[protein]
a 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] = a thymidine in DNA + S-methyl-L-cysteinyl-[protein]

Cofactor

Zn(2+)

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

Similarity

Belongs to the MGMT family.

Keywords

Complete proteome   DNA damage   DNA repair   Methyltransferase   Reference proteome   Transferase  

Feature

chain  Probable methylated-DNA--protein-cysteine methyltransferase

Uniprot

A0A194PQA4   A0A194QY97   A0A3S2NH25   A0A2H1VHG3   S4PBA3   A0A212FQ31   + More
A0A2A4JPN0   A0A1Q3W732   B3ER72   V5L2G8   A0A159ZQS5   A0A0U2K3R1   J2YBN4   A0A140E125   A0A1E1EU91   A0A0G2Y1B6   Q5UNU9   A0A0R2H085   A0A2A3HVM5   A0A3S0ZCS3   A0A2V8TG66   A0A1I0URB1   A0A2A2IHY2   A0A2U3BW07   A0A3N5UXR7   A0A0G2Y3R2   A0A0C2LU67   H8LPL4   A0A2A4FKQ0   A0A2T5RIP0   A0A2P1EL10   G0GYQ3   A0A0F3RCJ1   A0A3B7DB19   A0A2A8VC95   A0A0P4UTG9   A0A2E2TI68   A0A1H1FJM1   A0A0F3QBW9   A0A0F3QIH7   A0A1S5V092   A0A1Q4RVY6   Q1RIP0   A0A1C3EW44   A0A249E1N8   A0A124R6V0   A0A1R1JCR7   A0A0M5JGE0   A0A0B2FAT4   A0A117XBQ5   A0A3G6F101   H2EF51   A0A1Z4QWW2   A0A2P1PA98   A0A167R6J9   A0A127W461   E4Q357   K2BQY8   A0A382HFY1   A0A1R1HZ59   A0A398DLM1   A0A346DEA3   A0A316GLT5   M5QSV9   A0A1G5S5T3   A0A0D0RSG7   A0A0D0G975   A0A1X6MAK1   A0A1W1XNI1   A0A348WH92   A0A257QQL4   A0A2A4HV95   A0A2A3T0Z1   A0A398D5I4   A0A2V8BSB2   A0A1M3H0B0   A0A0J0YUH7   A0A0A8X0G3   A0A348PSG1   A0A259DDP9   A0A2H5AWJ8   A0A2G8REY0   A0A3N5J5V3   Q12TZ2   A0A3Q9B8H5   A0A0C2FQ74   A0A0B1QC13   A0A1G0G7Y3   A0A1B9AHG7   A0A2E2U192   A0A2V8D7D0   A0A3N5JI11   A0A3D8PJW4   A0A3M1YBR3   B9MNR0   A0A268J0X3   A0A1G0IRE6   A0A2G1YJQ1   A0A0K6GKE3   A0A081NXJ7  

EC Number

2.1.1.63

Pubmed

26354079    23622113    22118469    20023027    27330993    26635738    + More
23071316    24886672    21646533    21375749    15486256    26415554    29166641    29899444    29773836    30086797    21914880    26494835    28066355    16703114    27313286    21216991    23019650    23144421    24603481    30148503    29099490    19404327    27774985   

EMBL

KQ459595    KPI95626.1    KQ461154    KPJ08551.1    RSAL01000041    RVE50878.1    + More
ODYU01002578    SOQ40267.1    GAIX01006012    JAA86548.1    AGBW02000285    OWR55799.1    NWSH01000966    PCG73372.1    MKUM01000080    OJW68423.1    CP001102    ACE05724.1    KF493731    AHA45142.1    KU761889    AMZ03137.1    KT599914    ALR84315.1    AFYC01000005    EJN41102.1    KF959826    AMK62000.1    AP017644    AP017645    BAV61823.1    BAV62809.1    HQ336222    JN036606    KM982401    KM982403    ADO18794.1    AEJ34935.1    AKI79480.1    AKI81371.1    AY653733    JQBM01000002    OBHM01000005    KRN46287.1    SOB43214.1    NSGV01000002    PBC71782.1    RZJR01000001    RUS48981.1    QHXT01000021    PYT10260.1    FOJP01000022    SFA66599.1    NPOA01000001    PAV31611.1    NSKH01000024    PWI40960.1    RPPI01000117    RPJ26849.1    KM982402    AKI80433.1    JXCB01000004    KIJ78381.1    CP003375    AFD19879.1    MTZU01000020    PCE33238.1    QAXS01000018    PTV98217.1    MG807320    AVL94582.1    CP002912    AEK74861.1    LAOQ01000007    KJW04008.1    CP032049    AXU06696.1    NTUP01000013    PFA67722.1    BBWW01000001    GAP94866.1    PAMH01000008    MAZ38797.1    FNKD01000004    SDR01242.1    LAOI01000001    KJV89646.1    LAOJ01000001    KJV91961.1    KY110734    AQN68063.1    MRCD01000017    OKH52954.1    CP000087    ABE04774.1    LYMV01000121    ODA37617.1    CP016305    ASX27435.1    LOXM01000279    KVG53106.1    MTJZ01000012    OMG73157.1    CP012602    ALC92157.1    JUEI01000015    KHL96208.1    LOTN01000055    KUZ84541.1    CP033854    AYZ62163.1    JN885999    AEX63116.1    AP018280    BAZ42410.1    CP027845    AVP88188.1    KU877344    ANB50366.1    CP014616    AMQ08406.1    CP002216    ADQ03893.1    AMFJ01019640    EKD71303.1    UINC01060997    SVB86099.1    MTHC01000027    OMG51752.1    QXIW01000027    RIE13017.1    CP025535    AXM88431.1    QGGW01000003    PWK60879.1    ANMT01000020    JRZG01000007    EMI10325.1    KGP61385.1    FMWL01000022    SCZ81772.1    JXTH01000020    KIQ94597.1    JXTG01000003    KIP21885.1    NDEZ01000043    OSX53398.1    FWXF01000013    SMC25405.1    DMVW01000179    HAR53904.1    NCAP01000104    OYV50086.1    NWVD01000009    PCG07943.1    NVQX01000015    PBO81709.1    QXIT01000093    RIE07698.1    QHWH01000124    PYQ99003.1    MKUL01000037    OJW51112.1    JTDO01000002    KLT73749.1    BASE01000034    GAM13455.1    DMTL01000216    HAQ71722.1    NCHW01000478    OZA12089.1    CP025394    AUG76529.1    AWWI01000066    PIL20144.1    RPQS01000213    RPH92200.1    CP000300    ABE53084.1    CP032760    AZO96742.1    KN749036    KIH50670.1    JRFJ01000001    KHJ56370.1    MGXP01000020    OGT33719.1    MAYU01000023    OCA83288.1    PANA01000005    MAZ45033.1    QHWJ01000179    QHWI01000318    PYR21023.1    PYR46911.1    RPQS01000049    RPH95193.1    PIOD01000025    RDW15519.1    RFHL01000031    RMG75660.1    CP001393    ACM59589.1    NPDE01000068    PAE28035.1    MGYI01000155    OGT64723.1    NVRA01000043    PHQ63752.1    CYGZ01000003    CUA79204.1    JNVM01000024    KEQ23170.1   

Proteomes

UP000053268    UP000053240    UP000283053    UP000007151    UP000218220    UP000001227    + More
UP000240740    UP000241559    UP000241834    UP000007297    UP000240935    UP000240366    UP000241484    UP000201519    UP000240552    UP000241474    UP000274448    UP000001134    UP000051992    UP000218662    UP000267180    UP000182453    UP000218887    UP000245673    UP000280678    UP000240461    UP000031981    UP000007592    UP000217994    UP000244089    UP000289600    UP000000865    UP000033736    UP000258667    UP000220634    UP000052243    UP000229611    UP000199444    UP000033661    UP000033689    UP000240801    UP000185789    UP000001951    UP000094749    UP000215668    UP000064029    UP000187194    UP000060713    UP000031063    UP000065521    UP000280242    UP000290974    UP000241365    UP000071057    UP000006889    UP000187142    UP000256325    UP000245708    UP000011915    UP000030117    UP000199208    UP000032102    UP000032047    UP000194126    UP000192783    UP000216804    UP000218784    UP000247608    UP000036027    UP000031014    UP000264021    UP000239175    UP000231259    UP000276319    UP000001979    UP000268874    UP000030826    UP000092646    UP000226955    UP000248064    UP000248353    UP000256520    UP000007723    UP000215893    UP000182738    UP000028123   

Pfam

PF02870   Methyltransf_1N        + More
PF01035   DNA_binding_1
PF00107   ADH_zinc_N
PF16884   ADH_N_2
PF12833   HTH_18
PF02805   Ada_Zn_binding
PF00730   HhH-GPD

Interpro

IPR008332   MethylG_MeTrfase_N        + More
IPR036217   MethylDNA_cys_MeTrfase_DNAb       
IPR036631   MGMT_N_sf       
IPR014048   MethylDNA_cys_MeTrfase_DNA-bd       
IPR036388   WH-like_DNA-bd_sf       
IPR001497   MethylDNA_cys_MeTrfase_AS       
IPR041694   ADH_N_2       
IPR036291   NAD(P)-bd_dom_sf       
IPR013149   ADH_C       
IPR011032   GroES-like_sf       
IPR014190   PTGR1       
IPR020843   PKS_ER       
IPR016221   Bifunct_regulatory_prot_Ada       
IPR009057   Homeobox-like_sf       
IPR004026   Ada_DNA_repair_Zn-bd       
IPR035451   Ada-like_dom_sf       
IPR018060   HTH_AraC       
IPR023546   MGMT       
IPR011257   DNA_glycosylase       
IPR003265   HhH-GPD_domain       

SUPFAM

SSF53155   SSF53155        + More
SSF46767   SSF46767       
SSF50129   SSF50129       
SSF51735   SSF51735       
SSF46689   SSF46689       
SSF57884   SSF57884       
SSF48150   SSF48150       

Gene 3D

1.10.10.10     3.40.10.10    

CDD

cd06445  ATase
cd08294  leukotriene_B4_DH_like
cd00056  ENDO3c

ProteinModelPortal

A0A194PQA4   A0A194QY97   A0A3S2NH25   A0A2H1VHG3   S4PBA3   A0A212FQ31   + More
A0A2A4JPN0   A0A1Q3W732   B3ER72   V5L2G8   A0A159ZQS5   A0A0U2K3R1   J2YBN4   A0A140E125   A0A1E1EU91   A0A0G2Y1B6   Q5UNU9   A0A0R2H085   A0A2A3HVM5   A0A3S0ZCS3   A0A2V8TG66   A0A1I0URB1   A0A2A2IHY2   A0A2U3BW07   A0A3N5UXR7   A0A0G2Y3R2   A0A0C2LU67   H8LPL4   A0A2A4FKQ0   A0A2T5RIP0   A0A2P1EL10   G0GYQ3   A0A0F3RCJ1   A0A3B7DB19   A0A2A8VC95   A0A0P4UTG9   A0A2E2TI68   A0A1H1FJM1   A0A0F3QBW9   A0A0F3QIH7   A0A1S5V092   A0A1Q4RVY6   Q1RIP0   A0A1C3EW44   A0A249E1N8   A0A124R6V0   A0A1R1JCR7   A0A0M5JGE0   A0A0B2FAT4   A0A117XBQ5   A0A3G6F101   H2EF51   A0A1Z4QWW2   A0A2P1PA98   A0A167R6J9   A0A127W461   E4Q357   K2BQY8   A0A382HFY1   A0A1R1HZ59   A0A398DLM1   A0A346DEA3   A0A316GLT5   M5QSV9   A0A1G5S5T3   A0A0D0RSG7   A0A0D0G975   A0A1X6MAK1   A0A1W1XNI1   A0A348WH92   A0A257QQL4   A0A2A4HV95   A0A2A3T0Z1   A0A398D5I4   A0A2V8BSB2   A0A1M3H0B0   A0A0J0YUH7   A0A0A8X0G3   A0A348PSG1   A0A259DDP9   A0A2H5AWJ8   A0A2G8REY0   A0A3N5J5V3   Q12TZ2   A0A3Q9B8H5   A0A0C2FQ74   A0A0B1QC13   A0A1G0G7Y3   A0A1B9AHG7   A0A2E2U192   A0A2V8D7D0   A0A3N5JI11   A0A3D8PJW4   A0A3M1YBR3   B9MNR0   A0A268J0X3   A0A1G0IRE6   A0A2G1YJQ1   A0A0K6GKE3   A0A081NXJ7  

PDB

4WXC     E-value=4.47398e-11,     Score=156

KEGG

101737268    no KO assigned | (RefSeq) methylated-DNA--protein-cysteine methyltransferase

GO

GO:0003908   GO:0006281   GO:0032440   GO:0005737   GO:0047522   GO:0008270   GO:0003700   GO:0043565   GO:0006307   GO:0006284   GO:0003677   GO:0006355   GO:0003824   GO:0006508   GO:0031012   GO:0005089   GO:0035023   GO:0005515   GO:0016742  

Subcellular location

Cytoplasm  

Length:

79

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.0163

Exp number, first 60 AAs:

0.01628

Total prob of N-in:

0.10176

outside

1  -  79

 

 

Pi

76.548663

Theta

83.606199

Tajima's D

-0.264263

CLR

270.01167

CSRT

0.294335283235838

Interpretation

Uncertain