Gene

KWMTBOMO04592

Pre Gene Modal

BGIBMGA005487

Annotation

PREDICTED:_methyltransferase-like_protein_16_[Papilio_xuthus]

Location

Bomo_Chr8(+):10460260-10464886      View in EpiBrowser

Full name

U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase       + More
RNA N6-adenosine-methyltransferase mettl16      

Alternative Name

Methyltransferase 10 domain-containing protein
Methyltransferase-like protein 16
N6-adenosine-methyltransferase METTL16
U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase

Location in the cell

Nuclear   Reliability : 2.964

CDS

ATGGCAATGAATAGATTTATGCACCCTCGAAATATATATAAAACTCCACCGGACTTCATTCAATTGGCCAAAGATTTCTCAGAATTTTCAAAGATTACAAAAACTGATGTTACGGGGAAGGTTACTATTGACTTCAAAGACCCTCAATCACTGCGCGTATTAACTAAATGTTTGTTGAAAAAAGATTTTAACCTAGATGTGGAAATCCCATCAGATAGACTTGTACCCACACTTCCTTTAAGACTTAATTATATTTTGTGGATAGAAGATATATTGGCTGCTGTTGAAATATTCACAGATGTAAAGGGTTTAGATATAGGTACAGGAGCTTGTGCCATTTATCCACTGCTGGCAGCTGTAAAAAACCAATGGCTTATGTATGGTACAGAAACTGATGCAAACAGTTTGAAAGTAGCACAAGAAAATATACAAAAAAATAATCTCCAGGACTTAATAAAATTAAAAATAAACACCACAAGTTCTATAATTGATTATTTATTCACTGAAAATGAAACTGTATACTATGATTTTTGTATGTGCAACCCACCATTTTATAGTACAATGATGGAATTATGGGAGTCTAGGAGCCCAGCAAGACCTCCTCCTAAAAATGGTTTCACTGGATCACCTCAAGAATTAATAACTGAAGGTGGTGAATTAGAATTTTGTAGGAAACTTATTGCTGAGAGCAAAAAATATTGCTATAACATTTTAATCTTCACATCAATGATTGGTCACAAGTTTAATTTGGCTCAACTTATCGATGATTTGAAGTCAGATAATATTAATTATACACATACGGAGTTTTGTCAAGGACGAGTAACCAGATGGGGATTAGCTTGGACATTTCACGATTATGATCTACTGCAACTACTTCCCCCAAGAGATAAACCAAGAAAAAAGATTCAACCACTAGTTTTTCTTTTACCGGAACTTCCGAATTGTACATATGACTTAGCATCCGCTTCAAACAAAGTCAAAACCATTTTAGATAGATTAGGAATCGCTTATGAAGTTGTTGAAAAAAGAGGGAACACTATAAAAATAGATATGGTAGCAAAAGTAAATACGTGGTCTAACCAACGAAGAAAAAGACGTCAAGAAAAAAGGATGCAAGAAGAAACGGACGTAAAGAAGATGAAAAGTAATAATTTAGAATTAAATGATATGAAAAATGGTAGTTTTGAGATAACTGAAAATACTAGTTCACATGTACAAAGTAATGCAGAATTAGTTGTAAATAAGGTAATTGAAAAAAACAATGAAGACTCTAATGCAGTTAGAAGCACAGAATCAGAGCAAGAAGGATTATTAGGAATACAAGTACGTGCAGTTTTAAAAATATTCAAAAAAGATAATGAAATTCTGCTTGAAACAGAGTTCATCAATGGTACTTTAGGTAAAGAAGGATTGCATCAGATTGTACAGTTTATTAAAAATAATTGGAAATAA

Protein

MAMNRFMHPRNIYKTPPDFIQLAKDFSEFSKITKTDVTGKVTIDFKDPQSLRVLTKCLLKKDFNLDVEIPSDRLVPTLPLRLNYILWIEDILAAVEIFTDVKGLDIGTGACAIYPLLAAVKNQWLMYGTETDANSLKVAQENIQKNNLQDLIKLKINTTSSIIDYLFTENETVYYDFCMCNPPFYSTMMELWESRSPARPPPKNGFTGSPQELITEGGELEFCRKLIAESKKYCYNILIFTSMIGHKFNLAQLIDDLKSDNINYTHTEFCQGRVTRWGLAWTFHDYDLLQLLPPRDKPRKKIQPLVFLLPELPNCTYDLASASNKVKTILDRLGIAYEVVEKRGNTIKIDMVAKVNTWSNQRRKRRQEKRMQEETDVKKMKSNNLELNDMKNGSFEITENTSSHVQSNAELVVNKVIEKNNEDSNAVRSTESEQEGLLGIQVRAVLKIFKKDNEILLETEFINGTLGKEGLHQIVQFIKNNWK

Description

RNA N6-methyltransferase that methylates adenosine residues of a subset of RNAs and plays a key role in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts. Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs). In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs: requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure. In presence of S-adenosyl-L-methionine, binds the 3'-UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, impairing MAT2A expression. In S-adenosyl-L-methionine-limiting conditions, binds the 3'-UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, preventing N6-methylation and promoting expression of MAT2A. In addition to mRNAs, also able to mediate N6-methylation of U6 small nuclear RNA (U6 snRNA): specifically N6-methylates adenine in position 43 of U6 snRNAs.
RNA N6-methyltransferase that methylates adenosine residues at the N(6) position of a subset of RNAs and is involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts. Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs). In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs: requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure. Plays a key role in S-adenosyl-L-methionine homeostasis by mediating N6-methylation of MAT2A mRNAs, altering splicing and/or stability of MAT2A transcripts: in presence of S-adenosyl-L-methionine, binds the 3'-UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, impairing MAT2A expression. In S-adenosyl-L-methionine-limiting conditions, binds the 3'-UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, preventing N6-methylation and promoting expression of MAT2A. In addition to mRNAs, also able to mediate N6-methylation of U6 small nuclear RNA (U6 snRNA): specifically N6-methylates adenine in position 43 of U6 snRNAs.

Catalytic Activity

adenosine in U6 snRNA + S-adenosyl-L-methionine = H(+) + N(6)-methyladenosine in U6 snRNA + S-adenosyl-L-homocysteine
adenosine in mRNA + S-adenosyl-L-methionine = H(+) + N(6)-methyladenosine in mRNA + S-adenosyl-L-homocysteine

Similarity

Belongs to the methyltransferase superfamily. METTL16/RlmF family.

Keywords

Complete proteome   Methyltransferase   Nucleus   Reference proteome   RNA-binding   S-adenosyl-L-methionine   Transferase  

Feature

chain  RNA N6-adenosine-methyltransferase mettl16

Uniprot

A0A2H1X3C0   A0A3S2LCS8   A0A194PQJ7   A0A2A4IWU6   A0A194QSQ5   A0A212FPR2   + More
A0A0L7LNZ3   D7ELH7   A0A1Y1MP99   A0A1B6KEI6   N6U7V2   J3JY65   A0A1B6K228   A0A1B6G9E9   K1PM64   U4UGZ2   A0A336LZE0   A0A336N0C0   Q4RJN4   A0A1S4FM63   H3DKT1   A0A2P8Z9Y2   A0A067QRE0   A0A1S3HCC7   B0X8H0   B8A4Y6   V9KKH7   Q6DC64   A0A182MDG5   A0A1Q3F2P4   A0A3Q2CYS1   H2UTY0   G1KT98   A0A182HHI5   A0A182JRB3   A0A182J5W2   A0A182L518   A0A2D0STF2   A0A3P8WQJ9   A0A3Q2WVS5   A0A3P9CDR4   W5UIX1   A0A3P8QLT3   A0A3P8QN40   I3JN00   A0A1B0CWC5   B0X0J2   A0A3Q3Q6E9   A0A1W7RFV6   Q16VX3   A0A3B3R019   A0A3B3QZC6   A0A0P6CRA9   A0A0K8RZQ3   A0A146M8K2   A0A162T185   A0A0K8RUD2   H3BFI6   A0A1A8IFU9   E9GHX3   A0A3Q2YJ74   A0A3Q3IX70   A0A3B5ALG7   A0A1A8RGV2   H2LUX4   A0A151MD54   A0A182UM29   A0A2U9B549   A0A1U7S7B8   A0A1A7W9G8   A0A1A8DFR3   A0A1A8CRZ2   A0A1A7ZB62   A0A3P9MPF5  

EC Number

2.1.1.-

Pubmed

26354079    22118469    26227816    18362917    19820115    28004739    + More
23537049    22516182    22992520    15496914    29403074    24845553    23594743    24402279    21551351    20966253    24487278    25186727    23127152    26358130    17510324    29240929    25727380    26823975    9215903    21292972    17554307    22293439   

EMBL

ODYU01013104    SOQ59789.1    RSAL01000041    RVE50846.1    KQ459595    KPI95582.1    + More
NWSH01005989    PCG63764.1    KQ461154    KPJ08512.1    AGBW02000920    OWR55736.1    JTDY01000457    KOB77084.1    DS497866    EFA12179.1    GEZM01025343    JAV87504.1    GEBQ01030383    JAT09594.1    APGK01045628    KB741037    ENN74657.1    BT128189    AEE63150.1    GECU01002187    JAT05520.1    GECZ01010714    JAS59055.1    JH818292    EKC19969.1    KB632319    ERL92297.1    UFQS01000339    UFQT01000339    SSX02995.1    SSX23362.1    UFQT01002815    SSX34097.1    CAAE01015036    CAG11398.1    PYGN01000132    PSN53309.1    KK853109    KDR11241.1    DS232489    EDS42506.1    BX324162    JW865943    AFO98460.1    BC078220    AXCM01021994    GFDL01013222    JAV21823.1    APCN01002416    JT416108    AHH41931.1    AERX01052037    AJWK01032233    DS232239    EDS38153.1    GDAY02001400    JAV50027.1    CH477579    EAT38740.1    GDIP01010221    JAM93494.1    GBKD01001249    JAG46369.1    GDHC01003533    JAQ15096.1    LRGB01000024    KZS21810.1    GBKC01002080    JAG43990.1    AFYH01008542    AFYH01008543    AFYH01008544    HAED01009731    HAEE01006528    SBQ95943.1    GL732545    EFX80911.1    HAEH01003546    HAEI01008240    SBS04494.1    AKHW03006231    KYO22452.1    CP026245    AWO99059.1    HADW01001232    HADX01005149    SBP02632.1    HAEA01004432    SBQ32912.1    HADZ01017609    SBP81550.1    HADY01001243    HAEJ01008161    SBP39728.1   

Proteomes

UP000283053    UP000053268    UP000218220    UP000053240    UP000007151    UP000037510    + More
UP000007266    UP000019118    UP000005408    UP000030742    UP000007303    UP000245037    UP000027135    UP000085678    UP000002320    UP000000437    UP000075883    UP000265020    UP000005226    UP000001646    UP000075840    UP000075881    UP000075880    UP000075882    UP000221080    UP000265120    UP000264840    UP000265160    UP000265100    UP000005207    UP000092461    UP000261600    UP000008820    UP000261540    UP000076858    UP000008672    UP000000305    UP000264820    UP000261400    UP000001038    UP000050525    UP000075903    UP000246464    UP000189705    UP000265180   

PRIDE

Q6DC64   

Pfam

PF05971   Methyltransf_10        + More
PF00505   HMG_box
PF15247   SLBP_RNA_bind
PF00439   Bromodomain

Interpro

IPR017182   METTL16/PsiM        + More
IPR010286   METTL16/RlmF       
IPR029063   SAM-dependent_MTases       
IPR036910   HMG_box_dom_sf       
IPR009071   HMG_box_dom       
IPR038294   SLBP_RNA_bind_sf       
IPR029344   SLBP_RNA_bind       
IPR002052   DNA_methylase_N6_adenine_CS       
IPR036427   Bromodomain-like_sf       
IPR001487   Bromodomain       

SUPFAM

SSF53335   SSF53335        + More
SSF47095   SSF47095       
SSF47370   SSF47370       

Gene 3D

1.10.30.10     1.10.8.1120     1.20.920.10    

ProteinModelPortal

A0A2H1X3C0   A0A3S2LCS8   A0A194PQJ7   A0A2A4IWU6   A0A194QSQ5   A0A212FPR2   + More
A0A0L7LNZ3   D7ELH7   A0A1Y1MP99   A0A1B6KEI6   N6U7V2   J3JY65   A0A1B6K228   A0A1B6G9E9   K1PM64   U4UGZ2   A0A336LZE0   A0A336N0C0   Q4RJN4   A0A1S4FM63   H3DKT1   A0A2P8Z9Y2   A0A067QRE0   A0A1S3HCC7   B0X8H0   B8A4Y6   V9KKH7   Q6DC64   A0A182MDG5   A0A1Q3F2P4   A0A3Q2CYS1   H2UTY0   G1KT98   A0A182HHI5   A0A182JRB3   A0A182J5W2   A0A182L518   A0A2D0STF2   A0A3P8WQJ9   A0A3Q2WVS5   A0A3P9CDR4   W5UIX1   A0A3P8QLT3   A0A3P8QN40   I3JN00   A0A1B0CWC5   B0X0J2   A0A3Q3Q6E9   A0A1W7RFV6   Q16VX3   A0A3B3R019   A0A3B3QZC6   A0A0P6CRA9   A0A0K8RZQ3   A0A146M8K2   A0A162T185   A0A0K8RUD2   H3BFI6   A0A1A8IFU9   E9GHX3   A0A3Q2YJ74   A0A3Q3IX70   A0A3B5ALG7   A0A1A8RGV2   H2LUX4   A0A151MD54   A0A182UM29   A0A2U9B549   A0A1U7S7B8   A0A1A7W9G8   A0A1A8DFR3   A0A1A8CRZ2   A0A1A7ZB62   A0A3P9MPF5  

PDB

6DU5     E-value=3.42772e-71,     Score=683

KEGG

101744195    K11393 U6 snRNA m6A methyltransferase [EC:2.1.1.346] | (RefSeq) methyltransferase-like protein 16 ho

GO

GO:0008168   GO:0005634   GO:0003677   GO:0003723   GO:0070475   GO:0052907   GO:0003676   GO:0120048   GO:0010608   GO:0006402   GO:0061157   GO:0080009   GO:0006556   GO:0048024   GO:0030629   GO:0120049   GO:0001734   GO:0035613   GO:0016021   GO:0006886   GO:0008536   GO:0020037   GO:0016491   GO:0007165   GO:0015031   GO:0016020   GO:0016742   GO:0003824  

PANTHER

PTHR13393  

Subcellular location

Nucleus  

Length:

483

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.0890799999999999

Exp number, first 60 AAs:

0

Total prob of N-in:

0.01017

outside

1  -  483

 

 

Pi

174.063015

Theta

14.26429

Tajima's D

1.000093

CLR

0

CSRT

0.668016599170042

Interpretation

Uncertain