Gene

KWMTBOMO04532  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA005460

Annotation

cell_division_protein_[Bombyx_mori]

Location

Bomo_Chr8(+):9327157-9328350      View in EpiBrowser

Full name

Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase      

Alternative Name

2'-O-ribose RNA methyltransferase TRM7 homolog

Location in the cell

Cytoplasmic   Reliability : 1.28 Extracellular   Reliability : 1.482

CDS

ATGGGCAAGACATCCAAAGATAAGCGAGATATATATTACAGGCTTGCTAAAGAAGAAGGTTGGCGTGCACGTAGCGCATTTAAACTTTTGCAAATTAACGAGGAATACAACATATTTAATGGTGTACTGCGTGCAGTGGATCTTTGCGCAGCACCAGGCAGTTGGAGTCAAGTTTTAACCAAGAATCTTCGACAAAATGCTGTAAATACTGAGGATGTTAAAATAGTTGCTGTTGATCTTCAGGCGATGGCAGCATTACCGGGAGTGAAACAAATTCAAGGGGATATTACCAAACAAGAAACAGCAAATGCTATAATAGAAGAGTTCCAAGGCCTCAAAGCAGATTTGGTTGTATGTGATGGTGCACCAGATGTAACAGGCTTACATGATATTGATGAATATGTCCAATCTCAGTTATTACTCGCTGCATTGAACATAACAACCCATGTTCTTAAGAATGGAGGTACATTTGTGGCCAAAATATTCAGAGGAAAAGATGTTTCCCTGCTGTACTCACAATTAAAACAGTTTTTTAAACTTGTGACAGTATCCAAACCTAGGAGTTCAAGAAATTCGAGCATTGAAGCCTTTGTAATTTGTGAGAAATACAATCCCCCAGAAGATTATGTGCCGAATATGGTCAATCCACTGTTAGATCATAAATACTTTGATTTGGATTCTGATTTTAATAGTTTTACTGGAATAAATAGATTTATAGTGCCATTCAATGCTTGTGGAGATCTTAGTGCCTATGACTCGGATACATCATATTCTCTACTGCTTGAAGGACAAACATCTTATGAGTATAAAAATCCAATACAAAGTCCTATCAATCCGCCTTACAAAGATGTTTTGGAAAAAACAAAAAAAATGAAAATAAATCATGTTCCTAAATAA

Protein

MGKTSKDKRDIYYRLAKEEGWRARSAFKLLQINEEYNIFNGVLRAVDLCAAPGSWSQVLTKNLRQNAVNTEDVKIVAVDLQAMAALPGVKQIQGDITKQETANAIIEEFQGLKADLVVCDGAPDVTGLHDIDEYVQSQLLLAALNITTHVLKNGGTFVAKIFRGKDVSLLYSQLKQFFKLVTVSKPRSSRNSSIEAFVICEKYNPPEDYVPNMVNPLLDHKYFDLDSDFNSFTGINRFIVPFNACGDLSAYDSDTSYSLLLEGQTSYEYKNPIQSPINPPYKDVLEKTKKMKINHVPK

Description

Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs.

Catalytic Activity

cytidine(32)/guanosine(34) in tRNA + 2 S-adenosyl-L-methionine = 2'-O-methylcytidine(32)/2'-O-methylguanosine(34) in tRNA + 2 H(+) + 2 S-adenosyl-L-homocysteine

Similarity

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. TRM7 subfamily.

Uniprot

H9J7G8   Q1HQ89   A0A2H1VBJ4   S4P3M9   A0A212EJG3   A0A2W1BXK8   + More
A0A194QTY9   A0A194PN33   C9E269   A0A0L7LUQ1   D8VJ53   D8VJ30   D8VJ50   D8VJ10   D8VJ17   D8VJ21   D8VJ13   D8VJ23   D0VK13   D0VK11   D0VK20   D0VK33   D0VK27   D0VK21   D0VK25   D0VK24   A0A2P6KG06   T1J1K8   A0A0L8IES3   G3K9J1   G3K9M2   G3K9I3   G3K9L6   G3K9J7   G3K9K1   G3K9J2   A0A2L2YHR1   A0A2L2YFG8   G3K9K4   G3K9J4   G3K9L9   G3K9K7   G3K9J6   G3K9J9   G3K9I4   G3K9M0   G3K9L1   A0A1S3IAF9   A0A1S3H3X9   A0A087UZY3   A0A210R5C0   G3K9J3   K1QIU2   W5NFA4   G3K9L3   A0A3Q2FQ89   A0A3B4A3E6   V8P6T7   A0A2D4GYY0   A0A2D4HES9   A0A0K8RW81   A0A0F7Z2L4   A0A0B8RP91   A0A3B3CIC0   R7VKI1   G3K9J8   A0A1B6KG62   G3K9I8   G3K9L7   G3K9I9   A0A3B4A3W5   A0A3S1BMG4   A0A1B6CK10   A0A0P5KL34   A0A1B6F5K3   A0A0N8EGB1   C3ZIK7   A0A0P6G9D1   A0A0P5NZ93   A0A3B1JHN3   F6TQG1   A0A1Y1W4X9   A0A0N8CCT7   A0A0P6EZ21   A0A0B7AHG6   A0A3B4CFG4   A0A0B7AH47   E9G3S7   A0A3Q1GLJ2   Q4SE69   H3CYH4   V3Z4Z2   A0A2R7X0A0   W5UAG5   A0A2G5BBF3   A0A2G8JB06   A0A3B3H689   A0A1A8QZR8   A0A1A8L6L4   A0A1A8E5F1  

EC Number

2.1.1.205

Pubmed

19121390    23622113    22118469    28756777    26354079    26227816    + More
20442862    21841803    26561354    28812685    22992520    25463417    24297900    25727380    25476704    29451363    23254933    18563158    25329095    12481130    15114417    21292972    15496914    23127152    25977457    29023486    17554307   

EMBL

BABH01013833    DQ443163    ABF51252.1    ODYU01001661    SOQ38195.1    GAIX01006374    + More
JAA86186.1    AGBW02014484    OWR41598.1    KZ149904    PZC78385.1    KQ461154    KPJ08460.1    KQ459599    KPI94403.1    GQ452006    ACV83779.1    JTDY01000051    KOB79160.1    GQ987110    GQ987111    GQ987113    GQ987117    GQ987118    ADI81797.1    GQ987087    GQ987088    GQ987089    GQ987090    GQ987091    GQ987092    GQ987093    GQ987094    GQ987095    GQ987096    GQ987097    GQ987098    GQ987099    GQ987100    GQ987101    GQ987102    GQ987103    GQ987104    GQ987105    GQ987106    ADI81774.1    GQ987107    GQ987108    GQ987109    GQ987112    GQ987114    GQ987115    GQ987116    GQ987119    GQ987120    GQ987121    GQ987122    GQ987123    GQ987124    GQ987125    GQ987126    ADI81794.1    GQ987067    GQ987068    GQ987069    GQ987071    GQ987072    GQ987073    GQ987075    GQ987076    GQ987077    GQ987079    GQ987081    GQ987082    GQ987083    GQ987084    GQ987085    GQ987086    ADI81754.1    GQ987074    ADI81761.1    GQ987078    ADI81765.1    GQ987070    ADI81757.1    GQ987080    ADI81767.1    GU078084    GU078109    ACY09322.1    ACY09347.1    GU078082    GU078083    GU078085    GU078086    GU078087    GU078088    GU078089    GU078090    GU078105    GU078106    GU078107    GU078108    ACY09320.1    ACY09321.1    ACY09323.1    ACY09324.1    ACY09325.1    ACY09326.1    ACY09327.1    ACY09328.1    ACY09343.1    ACY09344.1    ACY09345.1    ACY09346.1    GU078091    GU078093    GU078094    GU078097    GU078099    GU078100    GU078101    GU078102    GU078103    ACY09329.1    ACY09331.1    ACY09332.1    ACY09335.1    ACY09337.1    ACY09338.1    ACY09339.1    ACY09340.1    ACY09341.1    GU078104    ACY09342.1    GU078098    ACY09336.1    GU078092    ACY09330.1    GU078096    ACY09334.1    GU078095    ACY09333.1    MWRG01012959    PRD25258.1    JH431789    KQ415942    KOF99535.1    JN174663    AEN68006.1    JN174694    AEN68037.1    JN174655    AEN67998.1    JN174677    JN174678    JN174680    JN174681    JN174682    JN174684    JN174687    JN174688    JN174693    AEN68020.1    AEN68021.1    AEN68023.1    AEN68024.1    AEN68025.1    AEN68027.1    AEN68030.1    AEN68031.1    AEN68036.1    JN174657    JN174658    JN174659    JN174662    JN174669    JN174672    JN174674    JN174675    AEN68000.1    AEN68001.1    AEN68002.1    AEN68005.1    AEN68012.1    AEN68015.1    AEN68017.1    AEN68018.1    JN174673    AEN68016.1    JN174664    AEN68007.1    IAAA01022333    LAA06765.1    IAAA01022334    LAA06767.1    JN174676    JN174686    AEN68019.1    AEN68029.1    JN174666    JN174667    AEN68009.1    AEN68010.1    JN174691    AEN68034.1    JN174679    AEN68022.1    JN174668    AEN68011.1    JN174671    AEN68014.1    JN174656    AEN67999.1    JN174690    JN174692    AEN68033.1    AEN68035.1    JN174683    AEN68026.1    KK122529    KFM82922.1    NEDP02000262    OWF56175.1    JN174665    AEN68008.1    JH823221    EKC36697.1    AHAT01031519    JN174685    AEN68028.1    AZIM01000635    ETE70035.1    IACJ01150332    LAA64924.1    IACK01015183    IACK01015185    LAA70463.1    GBKC01001375    JAG44695.1    GBEX01003709    JAI10851.1    GBSH01002397    JAG66629.1    AMQN01004037    KB292507    ELU17391.1    JN174670    AEN68013.1    GEBQ01029529    JAT10448.1    JN174660    AEN68003.1    JN174689    AEN68032.1    JN174661    AEN68004.1    RQTK01000007    RUS91682.1    GEDC01023464    JAS13834.1    GDIQ01208173    GDIQ01208172    GDIQ01168832    LRGB01001463    JAK43553.1    KZS11920.1    GECZ01024289    JAS45480.1    GDIQ01241558    GDIQ01240015    GDIQ01230263    GDIQ01209474    GDIQ01209473    GDIQ01161489    GDIQ01126149    GDIQ01101712    GDIQ01067823    GDIQ01029580    JAN65157.1    GG666627    EEN47732.1    GDIQ01039438    JAN55299.1    GDIQ01135800    JAL15926.1    EAAA01001403    MCFD01000009    ORX68581.1    GDIQ01092363    JAL59363.1    GDIQ01057275    JAN37462.1    HACG01032500    CEK79365.1    HACG01032501    CEK79366.1    GL732531    EFX85822.1    CAAE01014625    CAG01063.1    KB203251    ESO85773.1    KK856221    PTY25227.1    JT410164    AHH38953.1    KZ303500    PIA16322.1    MRZV01002950    PIK32928.1    HAEH01000349    HAEI01006702    SBR98708.1    HAEF01003073    HAEG01000064    SBR40455.1    HADZ01006064    HAEA01012718    SBQ41198.1   

Proteomes

UP000005204    UP000007151    UP000053240    UP000053268    UP000037510    UP000053454    + More
UP000085678    UP000054359    UP000242188    UP000005408    UP000018468    UP000265020    UP000261520    UP000261560    UP000014760    UP000271974    UP000076858    UP000001554    UP000018467    UP000008144    UP000193922    UP000261440    UP000000305    UP000257200    UP000007303    UP000030746    UP000221080    UP000242474    UP000230750    UP000001038   

PRIDE

H9J7G8    Q1HQ89   

Pfam

PF01728   FtsJ

Interpro

IPR015507   rRNA-MeTfrase_E        + More
IPR002877   rRNA_MeTrfase_FtsJ_dom       
IPR028590   RNA_methyltr_E_Trm7       
IPR029063   SAM-dependent_MTases       

SUPFAM

SSF53335   SSF53335       

ProteinModelPortal

H9J7G8   Q1HQ89   A0A2H1VBJ4   S4P3M9   A0A212EJG3   A0A2W1BXK8   + More
A0A194QTY9   A0A194PN33   C9E269   A0A0L7LUQ1   D8VJ53   D8VJ30   D8VJ50   D8VJ10   D8VJ17   D8VJ21   D8VJ13   D8VJ23   D0VK13   D0VK11   D0VK20   D0VK33   D0VK27   D0VK21   D0VK25   D0VK24   A0A2P6KG06   T1J1K8   A0A0L8IES3   G3K9J1   G3K9M2   G3K9I3   G3K9L6   G3K9J7   G3K9K1   G3K9J2   A0A2L2YHR1   A0A2L2YFG8   G3K9K4   G3K9J4   G3K9L9   G3K9K7   G3K9J6   G3K9J9   G3K9I4   G3K9M0   G3K9L1   A0A1S3IAF9   A0A1S3H3X9   A0A087UZY3   A0A210R5C0   G3K9J3   K1QIU2   W5NFA4   G3K9L3   A0A3Q2FQ89   A0A3B4A3E6   V8P6T7   A0A2D4GYY0   A0A2D4HES9   A0A0K8RW81   A0A0F7Z2L4   A0A0B8RP91   A0A3B3CIC0   R7VKI1   G3K9J8   A0A1B6KG62   G3K9I8   G3K9L7   G3K9I9   A0A3B4A3W5   A0A3S1BMG4   A0A1B6CK10   A0A0P5KL34   A0A1B6F5K3   A0A0N8EGB1   C3ZIK7   A0A0P6G9D1   A0A0P5NZ93   A0A3B1JHN3   F6TQG1   A0A1Y1W4X9   A0A0N8CCT7   A0A0P6EZ21   A0A0B7AHG6   A0A3B4CFG4   A0A0B7AH47   E9G3S7   A0A3Q1GLJ2   Q4SE69   H3CYH4   V3Z4Z2   A0A2R7X0A0   W5UAG5   A0A2G5BBF3   A0A2G8JB06   A0A3B3H689   A0A1A8QZR8   A0A1A8L6L4   A0A1A8E5F1  

PDB

6EM5     E-value=6.21612e-38,     Score=394

KEGG

732894    K14864 tRNA (cytidine32/guanosine34-2'-O)-methyltransferase [EC:2.1.1.205] | (RefSeq) cell division

GO

GO:0002181   GO:0008175   GO:0002128   GO:0005737   GO:0051301   GO:0030488   GO:0001510   GO:0008168   GO:0008033   GO:0032259   GO:0006310   GO:0006418   GO:0009058   GO:0016742   GO:0003824  

PANTHER

PTHR10920:SF12  

Subcellular location

Cytoplasm  

Length:

298

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.06431

Exp number, first 60 AAs:

0.02444

Total prob of N-in:

0.22862

outside

1  -  298

 

 

Pi

280.541301

Theta

196.995234

Tajima's D

1.240209

CLR

0.722697

CSRT

0.72306384680766

Interpretation

Uncertain