Gene

KWMTBOMO04505  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA005449

Annotation

PREDICTED:_DNA_repair_protein_RAD50_[Bombyx_mori]

Location

Bomo_Chr8(+):8286478-8301636      View in EpiBrowser

Full name

DNA repair protein RAD50      

Location in the cell

Nuclear   Reliability : 3.84

CDS

ATGTTTCAGGCAAAGTACTTTAAATCATTACCCGGGGTTTCAAAAGCCATACTAAATTCTGTCGTATTTTGCCACCAAGAAGATTCAAGCTGGCCACTCGATGAAGGAAAAAAGGTTAAGGAAAGATTTGATGAAATTTTTGATGCTGATAAATACAGTGATTGCTTAGATCGTTTAAAAAAAATAAGAAAGGATTATGCACAGAACTTAAAGTTGCTTGAACAAGAAGTGGCTCATTTAACAGAGAAGAAACAAGATTTAGACAAGAAAAAACTTAATGTAGTTAATACAGAGACAAATATCTCCCAAACTGAAATAAAAGTAACAGAACTAACACAAGAATTAAAACCAGTTAATGAAAAATTAAGTGCTATCAAAACACTCGAGAACAACTTACTGAGTTATGAAACCAAACGAGAAAAAATTAAGATGAGACTAGAGCAAGCTCGAATAGAAGAGGAGAAACTAAAAAACAACATCAAGACTTTGTTTGAAGGAGCACTTGATGAATTGGAACAAAATATAAAAAACTACGCAACTACAGTTAAAGCTAAACAAAAAGAGCTTGAAGATTCATACAAAAAGAACACATCATTCAACAAGGAAGAAGAAAAAATTGCCAATGAGAAGTCTTCAAACGAGATAGAGTACAACAAATTTATATTATTGGAAAGTCAAAATCAAGAGAAGATTGATAAGAGAAATGAACAGATTGTCGAAACTGCAAAAATTGCTGAAATTGAAAACATCACTGAAGTTCAAACAAACGAGGAAGCAGAGAATGCCACAAAAACTATTTTGGAAAAAGTAGAAAGTCTAAAAAGTGACCATAAAGTGCAAAAATCACAAGCTGATGAAGAAGAAAAGAAAGCGCAGGGATTCGTTGATGAATGTCGTGTAGCCTTGTCTGGTCATACACAAAAAATCAGTAGCAAAGAAGGAGAGATAAATAAGAAGAAAAAAGAGATTACTAAAATAGAAAAAGATATAACATCAGCAAATCAATCAAAAGATAAACTAGAATTGATTGAAAAGAAGTTGCTTGCAGTTGAAGAAGAACACAAACAAGCTGAAAACGAATTAAACATTGAAGAATGTCAGCAAGAAATCAACGAAGATGAGAAAGCTGTGGAACAATATGAACAAGAAATGGATGAGCTTAGTCAAAAGATAACCAAGCTTCAAAAGCAAACTGCTAAATTGAAAGAAAAGGAAATGATCGAAGAGAATCTCAAAACTAAAGAAAAACAGTTAATTGTTCTTAAAAACAAACACAAGACGGCCCTGATGGAACTGCTTGGTAATGTACCCGAAAAAGACTTTGCTGTGACAATCAACAAATTTGAGTGTGAAGTGCGAAATGAAGTTGAAACACTGAAGAAAAAATTGAAAGAAAAACAGATAGAGATAACGACTTTGGAAGCGGAGCGAAAACATATTCGTGAATCTTTAAACGAACGACGCAGTGAACTGACTAAAGCCGAAGACAAAATATACAAGGCATGCGGAACCCAGCCCTATGATGTTACGTTATCTAAATATACAACCACAGTAGAAAAACTTCAGGATGAACAAAATGTTTTACAATCTTCAATGTTCATCATAAACAAATATAAAGGGCAACTTAAAGATAACAGTTGCTGTCCATTATGCAATCGCGGCTTTGAAAACGAGTCTGAGGTAACCGACTTAGTAACACAACTAACAACTCAAGTTATGAATGTGCCGGGTAAACTTGAAAAAGTTACTGAAGATCTCCAAAGAGCGTCAGCTAAAAAAGATGAAATACTCAGTATGAAGTCTTTAAATGAGAAAATAACCACTCTTAAAGATACGGAAATCCCCCAGCTGGAGAAACGAATCGTTGATGTTGATACTCAAATTAAGACTCTAAATGAAACTGTGGAAGAAATAACCATGTCTCTGATTGAACCAGAGCAAAAGATGACCACTGCTAAGCAAATACACGGGGATATGCCGATGCTTGACCGCTATATACAAGAAATTAAAACGGTCACCAAAAAGTTTGAGGCAGTAAAAGAACAATGTGGCGATGTTGATTCAGAAATGACGTTGGATGTTGCGACTGCCAAGCAAGTGGATTTTAAGCAAAAAATTAGCACCCTAAGGAATCGCGTAAAAACCACACAGAAGAAACTAAATGCACACAACAAGAAACTGCAAACTTTATATGAAAAAAAAACTAAAGCTAAGGAAGAATTGTTAAATACACAGAAAAAGGTTCAAGACATCGTAAATCTCGAGGAATCTAAAAAGCAACTGCAATCAGATTGTGAGAGACTAGAAACCGAACTAAAAGAACTGAAAGAAGCTATAACCCCACTGGAAGTCGCACTTAAAGAAAAAATCGAGGCTAAGACCGAAGTAGTGAGAAGGAACAGAGATTTGATTGAGGCTGGAAGTGCCTATATTGCAAAAGTCGAAGTAGCTTTCAATAAAGTAAAGTCTCTCGATGCTGAGATCCAACAGCATAAAAAAAAGAATGTACCCCGTGAAATGGAGAAGATACAAGAAGCAAACGATAAATTAATGGAAAAACAAAAACAAATCATGACTGACAGACAAGTGCTCACAAAAAAAATTGATACTCTTAAAGATGAAATTGCAAAGCAAGAGGTTTATAAACGTGATCTAGATGACAACTTGTGTTTGCGTAAAGCACAAACGGACATCACAAATTGGCAAAAACAAGATGATGAATTGAATGAAAAATTGAATGGTATTAACAAAGAAGAATTAAATGAAAAAGAATCATTAATCATTAAGCAAACGAAGTTGTTCCAACAGAAGGCAGAGTGCACTGGAGCATTGAGTGAACTTAAGAAAAGTTTAAAAGGCTTCAGGAATGAACTGGAAAAATCTTTATCTAAGGATATAGAAAAAAAATTTAGAGAAAAAATGTATGAACTACATGTAACTAAAGCTATTGACAAAGATATAAGGGAATATGCTGTTGCTTTAGATAAATGTCTTATGGAGTTTCATAGAGAGAAAATGGAAAATATCAACATGATTATCAGGGAACTTTGGCGAAAGATATACAGAGGTAATGATATTGATTACATTGAAATCAAGACTGAAGGCAACTTAACTGTAGAATCAGAACGACGCAAATATGACTACAGAGTAGTACAATCTAAAAACGGTGTGGAAATCGACATGCGTGGAAGATGTAGTGCTGGTCAAAAAGTACTCGCTTGCCTAATAATAAGGCTAGCACTTGCAGAGACATTCAGTTCCAGGTTTGGAATACTTGCTTTGGATGAACCAACAACAAATTTGGATCAAGAAAACATTCACAGTTTGTGTGCAGCCCTTGGTGAAATAGTCCAAGAGCGAATGATGCAAAAAAACTTTATGTTTATTATTATCACCCATGACAAAGAATTTATAGAGTCACTTGGGAACATTGATAAAGTTACACACTATTATGAGGTGTCTCGAAATGACAATGGCAAATCTAGGGTCAAGAGGATTAGATTTATGTAG

Protein

MFQAKYFKSLPGVSKAILNSVVFCHQEDSSWPLDEGKKVKERFDEIFDADKYSDCLDRLKKIRKDYAQNLKLLEQEVAHLTEKKQDLDKKKLNVVNTETNISQTEIKVTELTQELKPVNEKLSAIKTLENNLLSYETKREKIKMRLEQARIEEEKLKNNIKTLFEGALDELEQNIKNYATTVKAKQKELEDSYKKNTSFNKEEEKIANEKSSNEIEYNKFILLESQNQEKIDKRNEQIVETAKIAEIENITEVQTNEEAENATKTILEKVESLKSDHKVQKSQADEEEKKAQGFVDECRVALSGHTQKISSKEGEINKKKKEITKIEKDITSANQSKDKLELIEKKLLAVEEEHKQAENELNIEECQQEINEDEKAVEQYEQEMDELSQKITKLQKQTAKLKEKEMIEENLKTKEKQLIVLKNKHKTALMELLGNVPEKDFAVTINKFECEVRNEVETLKKKLKEKQIEITTLEAERKHIRESLNERRSELTKAEDKIYKACGTQPYDVTLSKYTTTVEKLQDEQNVLQSSMFIINKYKGQLKDNSCCPLCNRGFENESEVTDLVTQLTTQVMNVPGKLEKVTEDLQRASAKKDEILSMKSLNEKITTLKDTEIPQLEKRIVDVDTQIKTLNETVEEITMSLIEPEQKMTTAKQIHGDMPMLDRYIQEIKTVTKKFEAVKEQCGDVDSEMTLDVATAKQVDFKQKISTLRNRVKTTQKKLNAHNKKLQTLYEKKTKAKEELLNTQKKVQDIVNLEESKKQLQSDCERLETELKELKEAITPLEVALKEKIEAKTEVVRRNRDLIEAGSAYIAKVEVAFNKVKSLDAEIQQHKKKNVPREMEKIQEANDKLMEKQKQIMTDRQVLTKKIDTLKDEIAKQEVYKRDLDDNLCLRKAQTDITNWQKQDDELNEKLNGINKEELNEKESLIIKQTKLFQQKAECTGALSELKKSLKGFRNELEKSLSKDIEKKFREKMYELHVTKAIDKDIREYAVALDKCLMEFHREKMENINMIIRELWRKIYRGNDIDYIEIKTEGNLTVESERRKYDYRVVQSKNGVEIDMRGRCSAGQKVLACLIIRLALAETFSSRFGILALDEPTTNLDQENIHSLCAALGEIVQERMMQKNFMFIIITHDKEFIESLGNIDKVTHYYEVSRNDNGKSRVKRIRFM

Description

Essential component of the MRN complex, a complex that possesses single-stranded DNA endonuclease and 3' to 5' exonuclease activities, and plays a central role in double-strand break (DSB) repair. The complex participates in processes such as DNA recombination, DNA repair, genome stability, telomere integrity and meiosis. The MRN complex may protect telomeres by facilitating recruitment of HOAP and HP1 at chromosome ends. In the complex, it mediates the ATP-binding and is probably required to bind DNA ends and hold them in close proximity.
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation (By similarity).

Cofactor

Zn(2+)

Subunit

Homodimer. Probable component of the MRN complex with mre11 (By similarity).
Component of the MRN complex composed of two heterodimers RAD50/MRE11 associated with a single NBN. As part of the MRN complex, interacts with MCM8 and MCM9; the interaction recruits the complex to DNA repair sites. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11 and NBN. Found in a complex with TERF2. Interacts with RINT1. Interacts with BRCA1 via its N-terminal domain. Interacts with DCLRE1C/Artemis. Interacts with MRNIP.

Similarity

Belongs to the SMC family. RAD50 subfamily.

Keywords

ATP-binding   Cell cycle   Chromosome   Coiled coil   Complete proteome   DNA damage   DNA repair   Hydrolase   Meiosis   Metal-binding   Nucleotide-binding   Nucleus   Reference proteome   Telomere   Zinc   Acetylation   Alternative splicing   Direct protein sequencing   Phosphoprotein  

Feature

chain  DNA repair protein RAD50
splice variant  In isoform 3.

Uniprot

H9J7F7   A0A194PM06   A0A212EI90   A0A2A4J953   A0A194RCL8   A0A067QZL2   + More
A0A2Z5TRB2   A0A2J7QQY9   A0A2J7QQZ7   A0A2J7QQZ3   A0A1B6F1S1   A0A1B0GH32   A0A0V0G504   A0A2C9JC49   A0A1B6KQF5   A0A1B6C3H1   A0A069DZS5   A0A1B6D4X3   A0A224X5C5   A0A0A1WNN8   A0A0L8GKX7   B4P7X9   A0A1Q3F5U9   A0A182KXV5   A0A0J9U8E7   A0A182YG56   B0WAP9   A0A182QKZ8   A0A0K8UNW1   A0A3B0J5K5   A0A0K8VRS2   E0W0B2   B3NNP5   A0A3B0J5T6   B4MJF7   W8C472   A0A1W4VQG8   W5MWI4   A0A0B4K7L0   D0Z764   A0A1I8NB16   A0A1I8P2K6   A0A3Q0J5N7   A0A3Q0J6C2   V9KA54   Q9W252   A0A3B4DBX2   A0A182G7F0   A0A2I0LTA5   A0A3B4D7T9   B4I847   A0A1U7RTA8   A0A182P3Y5   Q5SV02   A0A3Q3N8A8   P70388   A0A151M734   A0A1A9WRR6   A0A1U7QKB1   A0A084VEJ8   A0A0L0CTW8   F1NEG1   B7NZL1   W5LKZ2   A0A3Q3AN83   A0A087RF50   K9IUU3   A0A182JDN9   A0A0B7AG76   B4QH58   A0A091GX79   F7AR56   A0A3P8TN45   A0A091RC47   A0A3Q1BHM8   A0A091IN49   A0A3Q1BDK4   A0A3B4WWW8   F6RT86   A0A3B5A3G4   A0A3B3QVD8   A0A099YQH8   A0A093C8H2   T1JCH0   A0A093R5B6   A0A0P6J401   A0A3B3QW23   G3V9X6   A0A146P4S6   A0A3B3QSS0   A0A3Q3FJ57   G3WB07   A0A146NW52   A0A1A7WGK8   A0A3P8TJH3   A0A2K6V0L4   A0A2S2QS01   A0A3Q2P9Q3  

EC Number

3.6.-.-

Pubmed

19121390    26354079    22118469    24845553    26760975    15562597    + More
26334808    25830018    17994087    17550304    20966253    22936249    25244985    20566863    24495485    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    25315136    24402279    18984573    12537569    15296753    15135728    26483478    23371554    12040188    17525332    19468303    21183079    8910585    16141072    15489334    10377422    12208847    22293439    24438588    26108605    15592404    25329095    19892987    17495919    29240929    15057822    15632090    21709235   

EMBL

BABH01013770    BABH01013771    BABH01013772    KQ459599    KPI94471.1    AGBW02014681    + More
OWR41199.1    NWSH01002530    PCG68064.1    KQ460397    KPJ15214.1    KK853069    KDR11819.1    FX985773    BBA93660.1    NEVH01012082    PNF31004.1    PNF31005.1    PNF31009.1    GECZ01025672    GECZ01010895    JAS44097.1    JAS58874.1    AJWK01003905    GECL01003006    JAP03118.1    GEBQ01026304    GEBQ01016094    JAT13673.1    JAT23883.1    GEDC01029242    JAS08056.1    GBGD01000130    JAC88759.1    GEDC01016549    JAS20749.1    GFTR01008729    JAW07697.1    GBXI01013628    JAD00664.1    KQ421376    KOF77598.1    CM000158    EDW92134.2    GFDL01012100    JAV22945.1    CM002911    KMY95790.1    DS231874    EDS41690.1    AXCN02001185    GDHF01024116    JAI28198.1    OUUW01000001    SPP75063.1    GDHF01011069    JAI41245.1    DS235858    EEB19068.1    CH954179    EDV55602.2    SPP75062.1    CH963846    EDW72246.1    GAMC01009761    JAB96794.1    AHAT01021397    AE013599    AFH08214.1    BT100311    ACZ52623.1    JW862011    AFO94528.1    BT003555    FJ219324    FJ219329    FJ219330    FJ219331    FJ219332    FJ219333    FJ219334    FJ219335    FJ219336    FJ219337    FJ219338    FJ219339    FJ219340    FJ219341    FJ219342    FJ219343    FJ219344    FJ219345    FJ219346    FJ219347    FJ219348    FJ219349    FJ219350    AY052106    JXUM01046519    KQ561480    KXJ78447.1    AKCR02000104    PKK20650.1    CH480824    EDW56772.1    AL645741    CH466575    EDL33574.1    U66887    AK018001    AK087982    BC058180    AKHW03006437    KYO20250.1    ATLV01012278    KE524778    KFB36392.1    JRES01000027    KNC34829.1    AC159985    DP001045    ACK28136.1    KL226335    KFM12104.1    GABZ01000350    JAA53175.1    HACG01032782    CEK79647.1    CM000362    EDX08204.1    KL513254    KFO87137.1    KK717443    KFQ37393.1    KK500767    KFP10124.1    KL885134    KGL72549.1    KL460192    KFV12235.1    JH432064    KL224978    KFW66199.1    GEBF01006495    JAN97137.1    AC135771    CH473948    EDM04395.1    GCES01147361    JAQ38961.1    AEFK01014865    AEFK01014866    AEFK01014867    AEFK01014868    AEFK01014869    AEFK01014870    GCES01150349    JAQ35973.1    HADW01003296    SBP04696.1    GGMS01010699    MBY79902.1   

Proteomes

UP000005204    UP000053268    UP000007151    UP000218220    UP000053240    UP000027135    + More
UP000235965    UP000092461    UP000076420    UP000053454    UP000002282    UP000075882    UP000076408    UP000002320    UP000075886    UP000268350    UP000009046    UP000008711    UP000007798    UP000192221    UP000018468    UP000000803    UP000095301    UP000095300    UP000079169    UP000261440    UP000069940    UP000249989    UP000053872    UP000001292    UP000189705    UP000075885    UP000000589    UP000261640    UP000050525    UP000091820    UP000189706    UP000030765    UP000037069    UP000000539    UP000018467    UP000264800    UP000053286    UP000075880    UP000000304    UP000002281    UP000265080    UP000257160    UP000053119    UP000261360    UP000002280    UP000261400    UP000261540    UP000053641    UP000054081    UP000002494    UP000007648    UP000233220    UP000265000   

PRIDE

H9J7F7    D0Z764    Q9W252    P70388    F1NEG1   

Pfam

PF13476   AAA_23        + More
PF04423   Rad50_zn_hook
PF13304   AAA_21
PF13175   AAA_15
PF00727   IL4

Interpro

IPR013134   Zn_hook_RAD50        + More
IPR038729   Rad50/SbcC_AAA       
IPR027417   P-loop_NTPase       
IPR004584   Rad50_eukaryotes       
IPR003959   ATPase_AAA_core       
IPR041685   AAA_15       
IPR002354   IL-4       
IPR009079   4_helix_cytokine-like_core       

SUPFAM

SSF52540   SSF52540        + More
SSF47266   SSF47266       

ProteinModelPortal

H9J7F7   A0A194PM06   A0A212EI90   A0A2A4J953   A0A194RCL8   A0A067QZL2   + More
A0A2Z5TRB2   A0A2J7QQY9   A0A2J7QQZ7   A0A2J7QQZ3   A0A1B6F1S1   A0A1B0GH32   A0A0V0G504   A0A2C9JC49   A0A1B6KQF5   A0A1B6C3H1   A0A069DZS5   A0A1B6D4X3   A0A224X5C5   A0A0A1WNN8   A0A0L8GKX7   B4P7X9   A0A1Q3F5U9   A0A182KXV5   A0A0J9U8E7   A0A182YG56   B0WAP9   A0A182QKZ8   A0A0K8UNW1   A0A3B0J5K5   A0A0K8VRS2   E0W0B2   B3NNP5   A0A3B0J5T6   B4MJF7   W8C472   A0A1W4VQG8   W5MWI4   A0A0B4K7L0   D0Z764   A0A1I8NB16   A0A1I8P2K6   A0A3Q0J5N7   A0A3Q0J6C2   V9KA54   Q9W252   A0A3B4DBX2   A0A182G7F0   A0A2I0LTA5   A0A3B4D7T9   B4I847   A0A1U7RTA8   A0A182P3Y5   Q5SV02   A0A3Q3N8A8   P70388   A0A151M734   A0A1A9WRR6   A0A1U7QKB1   A0A084VEJ8   A0A0L0CTW8   F1NEG1   B7NZL1   W5LKZ2   A0A3Q3AN83   A0A087RF50   K9IUU3   A0A182JDN9   A0A0B7AG76   B4QH58   A0A091GX79   F7AR56   A0A3P8TN45   A0A091RC47   A0A3Q1BHM8   A0A091IN49   A0A3Q1BDK4   A0A3B4WWW8   F6RT86   A0A3B5A3G4   A0A3B3QVD8   A0A099YQH8   A0A093C8H2   T1JCH0   A0A093R5B6   A0A0P6J401   A0A3B3QW23   G3V9X6   A0A146P4S6   A0A3B3QSS0   A0A3Q3FJ57   G3WB07   A0A146NW52   A0A1A7WGK8   A0A3P8TJH3   A0A2K6V0L4   A0A2S2QS01   A0A3Q2P9Q3  

PDB

5DA9     E-value=8.55226e-40,     Score=416

KEGG

101744768    K10866 DNA repair protein RAD50 [EC:3.6.-.-] | (RefSeq) DNA repair protein RAD50

GO

GO:0046872   GO:0030870   GO:0006281   GO:0000723   GO:0016887   GO:0005524   GO:0006302   GO:0016233   GO:0000722   GO:0008104   GO:0000793   GO:0032508   GO:0070192   GO:0000784   GO:0007004   GO:0000794   GO:0035861   GO:0051880   GO:0090305   GO:0043047   GO:0000790   GO:0004017   GO:0003691   GO:0003690   GO:0051321   GO:0000781   GO:0051276   GO:0005634   GO:0005654   GO:0032206   GO:0045120   GO:0007346   GO:0033674   GO:0016234   GO:0003677   GO:0000019   GO:0031954   GO:0006974   GO:1904354   GO:0030674   GO:0031860   GO:0046597   GO:0051291   GO:0048471   GO:0006310   GO:0006955   GO:0005136   GO:0005576   GO:0008083   GO:0006418   GO:0009058   GO:0016742   GO:0003824  

Subcellular location

Nucleus  
Chromosome  
Telomere  

Length:

1169

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00717999999999999

Exp number, first 60 AAs:

0.00018

Total prob of N-in:

0.00001

outside

1  -  1169

 

 

Pi

187.04012

Theta

183.74862

Tajima's D

0.025857

CLR

0.277492

CSRT

0.370381480925954

Interpretation

Uncertain