Gene

KWMTBOMO04502  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA005448

Annotation

replication_protein_A1_[Bombyx_mori]

Location

Bomo_Chr8(+):8250188-8259274      View in EpiBrowser

Full name

Replication protein A subunit       + More
Replication protein A 70 kDa DNA-binding subunit      

Alternative Name

Replication factor A protein 1
Single-stranded DNA-binding protein

Location in the cell

Cytoplasmic   Reliability : 1.017 Nuclear   Reliability : 1.083

CDS

ATGTCTTATAATTTGTCGGAGGGTGCCTTAGAGACCATAATGAAGGGTGGTAATTATGATGCACCAATAATACAAATGCTGGGTACAAAGAAGATTCAAGGGAATGATGCTGGGGATAGATACAGATTATTGGTATCAGATGGAAAATATACATATAGTTTTGCAATGTTAGCCACACAGTTGAATATTATGTTGCTCTCTGGAGAACTTACAGATTACTCTGTGATACAAGTTGATCAGTTTGTTACGACATTGCTGAAGAATGTTGGGAAAGGTGAAAAGCGTGTTATTATCATTCTAGCTTTGACCATTATAGCAAATGGTCGAGATGTTCGTCATAAGATAGGTAATCCACAACCATGGTCAGAGGAAGCAACATCAGCTCAACGCCCAGAACCAAAGCCTGCACCAGTAACTCCAAAGTTTACGGCTAAAGTAGAACCAAAAGCTGCTAATCTCGATTCCAGTATGCTCTCGGTTAAAATGACCAATCCAATCTCTAGCCTTAGTCCTTATCAAAATAAATGGGTAATAAGAGCTCGTGTAATGTCAAAAACACCTATAAGAACATGGAGTAATGCCAAAGGGGAAGGAAAACTATTTAGTATGTATCTATGTGATGAAACTGGAGAAATTAGAGCCACAGCTTTCAAAAACGAATGTGACACATTCTATGATATGATTCAGGTGGATAAAGTATACTACATAAGTCGGTGTGCAATCAAAACTGCCAATAAGAAGTTTACAACATTGAAGAATGATTATGAGATGACATTCACTGCTGAGACTGTCGTGGGTGAATATGTTGGAGATGAGTCTGCTGTGCCAACAGTCCAGTATGACTTTTTACCGATCAGCGAGATTGCTAATAAAGAAACTGAAACGCTGATAGATCTCATAGGTGTTTGCAAGTCAGCATCAGATGTCCAAGAGTTAACAGCTAAAAGTACTGGGAAACTACTTAAGAAAAGAGAAGTGATGCTAGTTGACGCTTCTGGTGGAGCTGTAACACTAACACTGTGGGGCAATGAAGCTGAAAAGTTTGAAGGAAACACAAACCCAATTTTAGCAGTAAAGGGAGCTCGTCTAACTGAATTCAACGGCAGTAAGTCATTGTCTTGTTTATCGAGCACAATGCTGCGTTTGAACCCCGATGTACCTGAAGCTCACAAACTAAGAGGCTGGTACGACAACGGAGGGGCAGATGCCGACATCGTCAACATATCTGCTAAGTCGGGTAACTTCACGGGTGGTTCGAATGAATGGATATCGTTTTCGGAAGCCGAGAGCCGTCAACTGGGCACCGGTGAGAAAGGCGATTACTACAGTCTCCTCGGCGTATTGACGTTTACGTTTTCCGAAAATGCCGTGTACAAGGCATGTCCGCAAGAACAGTGCAACAAGAAACTTGTCGATCAAGAAAATGGCCTATTCCGATGCGAGAAATGTAATAGAGAATATCCTAATTACAAACACAGGATCTTATTGGCGGCAAACGTCTCAGATCCTACTGGCGACCAACGTGTCACGCTTTTCAACGAAGCCGCAGAAACAATGCTGGGCAAGTCCGCCGAAGAAATCGCACGTCTGTCGGAATACGACAAGAACGAATATAACAAAATCTTTGACGAAGTGAAATTCAAAACTTTTGTCTTTAAATTCCGTACCAAGATGGAAAATTTTAATGATGAATCCAGGCTGAAGACGACCGTAGTTAACGTACAACCCGTCGACTATAAGGACGGTATTGCCAGACTTCTCAAGAACATAAAAACGGCCACTGGAGTACAAGTTTAA

Protein

MSYNLSEGALETIMKGGNYDAPIIQMLGTKKIQGNDAGDRYRLLVSDGKYTYSFAMLATQLNIMLLSGELTDYSVIQVDQFVTTLLKNVGKGEKRVIIILALTIIANGRDVRHKIGNPQPWSEEATSAQRPEPKPAPVTPKFTAKVEPKAANLDSSMLSVKMTNPISSLSPYQNKWVIRARVMSKTPIRTWSNAKGEGKLFSMYLCDETGEIRATAFKNECDTFYDMIQVDKVYYISRCAIKTANKKFTTLKNDYEMTFTAETVVGEYVGDESAVPTVQYDFLPISEIANKETETLIDLIGVCKSASDVQELTAKSTGKLLKKREVMLVDASGGAVTLTLWGNEAEKFEGNTNPILAVKGARLTEFNGSKSLSCLSSTMLRLNPDVPEAHKLRGWYDNGGADADIVNISAKSGNFTGGSNEWISFSEAESRQLGTGEKGDYYSLLGVLTFTFSENAVYKACPQEQCNKKLVDQENGLFRCEKCNREYPNYKHRILLAANVSDPTGDQRVTLFNEAAETMLGKSAEEIARLSEYDKNEYNKIFDEVKFKTFVFKFRTKMENFNDESRLKTTVVNVQPVDYKDGIARLLKNIKTATGVQV

Description

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage.

Subunit

Component of the heterotrimeric canonical replication protein A complex (RPA).

Similarity

Belongs to the replication factor A protein 1 family.

Keywords

Complete proteome   DNA replication   DNA-binding   Metal-binding   Nucleus   Phosphoprotein   Reference proteome   Zinc   Zinc-finger  

Feature

chain  Replication protein A 70 kDa DNA-binding subunit

Uniprot

Q5TLD3   H9J7F6   A0A3S2TPC1   A0A1E1W3W0   A0A2A4J7W4   A0A2H1WY01   + More
A0A0N1PEW2   A0A212EIA5   A0A194PNA2   A0A067RKT9   A0A2Z5TR94   A0A2P8Y1D6   A0A2J7Q8Z5   B0W5Z1   A0A1B6CV81   A0A1Q3F4P1   A0A1S4FXA4   Q16KY9   A0A182QCD7   A0A182N5G1   Q7PXI6   A0A182HKU4   A0A182U3F6   U5EWX6   A0A182X9W4   A0A182JRW5   W5JEE8   A0A023EVB3   A0A182VRB5   A0A182RPL3   A0A182Y582   A0A182MBH5   A0A182FP30   A0A182P2E3   A0A182JAC3   A0A0K8VQU9   A0A034WC14   W8BK99   A0A0A9YUP1   A0A146L4T4   A0A023F2L9   A0A224XK33   A0A1B0G3S9   A0A069DVV0   A0A084VW44   A0A0P4VM31   A0A1B0A0G5   A0A2S2PYK9   A0A1B6HZS7   A0A1B0BMK5   A0A1I8MNT3   A0A0V0G459   A0A1A9Y0Z1   A0A164NPL0   B4M169   A0A2A3ERK1   A0A0P6BZA4   B4PT26   A0A1B6MN54   A0A0P6EY13   B3NZV7   A0A0M4F3V6   B4HLN6   B4QYN5   Q24492   A0A2H8TNF8   A0A0P6I084   A0A2R7W1L9   J9K097   T1HTA7   A0A154PH53   A0A1A9VAH6   B4K953   A0A1I8QBZ9   A0A1Y1NLC2   A0A232EXI6   A0A087ZZR0   V9IGY9   A0A0N0U3V3   B4JF42   A0A3B0JPG3   A0A0P5TR09   Q298Q4   B4G4G0   B3MSV9   A0A1A9W3T9   B4NAK2   A0A2S2PBM4   A0A1B0CNK6   V5IEU7   A0A2P6KVW8   A0A1W4VVD2   A0A131XU94   A0A0L7R1H7   A0A3B4ZVY3   R7V2A0   A0A336LJ18   A0A293LLH6   D7EJW6   A0A2R5LE87  

Pubmed

19121390    26354079    22118469    24845553    26760975    29403074    + More
17510324    12364791    14747013    17210077    20920257    23761445    24945155    25244985    25348373    24495485    25401762    26823975    25474469    26334808    24438588    27129103    25315136    17994087    17550304    10731132    12537572    12537569    18327897    28004739    28648823    15632085    25765539    23254933    18362917    19820115   

EMBL

AB194776    BAD69789.1    BABH01013768    BABH01013769    RSAL01000032    RVE51523.1    + More
GDQN01009422    JAT81632.1    NWSH01002530    PCG68061.1    ODYU01011926    SOQ57950.1    KQ459161    KPJ03587.1    AGBW02014681    OWR41201.1    KQ459599    KPI94468.1    KK852411    KDR24447.1    FX985742    BBA93629.1    PYGN01001052    PSN38056.1    NEVH01016945    PNF25051.1    DS231845    EDS35978.1    GEDC01019902    JAS17396.1    GFDL01012556    JAV22489.1    CH477930    EAT34982.1    AXCN02001172    AAAB01008987    EAA01767.4    APCN01000869    GANO01001221    JAB58650.1    ADMH02001372    ETN62732.1    GAPW01000540    JAC13058.1    AXCM01000442    GDHF01026422    GDHF01026344    GDHF01019659    GDHF01011058    JAI25892.1    JAI25970.1    JAI32655.1    JAI41256.1    GAKP01007614    JAC51338.1    GAMC01007388    JAB99167.1    GBHO01008781    JAG34823.1    GDHC01016462    JAQ02167.1    GBBI01003341    JAC15371.1    GFTR01007464    JAW08962.1    CCAG010008228    GBGD01000854    JAC88035.1    ATLV01017439    KE525168    KFB42188.1    GDKW01000438    JAI56157.1    GGMS01001280    MBY70483.1    GECU01027519    JAS80187.1    JXJN01016981    GECL01003294    JAP02830.1    LRGB01002849    KZS06135.1    CH940650    EDW67480.1    KZ288194    PBC33902.1    GDIP01007132    JAM96583.1    CM000160    EDW96487.1    GEBQ01002636    JAT37341.1    GDIQ01056417    JAN38320.1    CH954181    EDV49955.1    CP012526    ALC46337.1    CH480815    EDW43063.1    CM000364    EDX13793.1    Z70277    AE014297    AY069331    GFXV01003898    MBW15703.1    GDIQ01011454    JAN83283.1    KK854243    PTY13627.1    ABLF02041005    ACPB03012822    KQ434905    KZC11216.1    CH933806    EDW14466.1    GEZM01001745    JAV97545.1    NNAY01001758    OXU23008.1    JR047152    AEY60335.1    KQ435863    KOX70402.1    CH916369    EDV93323.1    OUUW01000007    SPP82813.1    GDIP01125757    JAL77957.1    CM000070    EAL27901.2    CH479179    EDW24508.1    CH902623    EDV30349.1    CH964232    EDW80816.1    GGMR01014244    MBY26863.1    AJWK01020464    GANP01009567    JAB74901.1    MWRG01004520    PRD30477.1    GEFM01006021    JAP69775.1    KQ414667    KOC64699.1    AMQN01006222    KB297895    ELU09821.1    UFQT01000027    SSX18134.1    GFWV01002940    MAA27670.1    DS497721    EFA12891.1    GGLE01003541    MBY07667.1   

Proteomes

UP000005204    UP000283053    UP000218220    UP000053268    UP000007151    UP000027135    + More
UP000245037    UP000235965    UP000002320    UP000008820    UP000075886    UP000075884    UP000007062    UP000075840    UP000075902    UP000076407    UP000075881    UP000000673    UP000075920    UP000075900    UP000076408    UP000075883    UP000069272    UP000075885    UP000075880    UP000092444    UP000030765    UP000092445    UP000092460    UP000095301    UP000092443    UP000076858    UP000008792    UP000242457    UP000002282    UP000008711    UP000092553    UP000001292    UP000000304    UP000000803    UP000007819    UP000015103    UP000076502    UP000078200    UP000009192    UP000095300    UP000215335    UP000005203    UP000053105    UP000001070    UP000268350    UP000001819    UP000008744    UP000007801    UP000091820    UP000007798    UP000092461    UP000192221    UP000053825    UP000261400    UP000014760    UP000007266   

PRIDE

Q5TLD3    H9J7F6    Q24492    V5IEU7   

Pfam

PF01336   tRNA_anti-codon        + More
PF04057   Rep-A_N
PF16900   REPA_OB_2
PF08646   Rep_fac-A_C

Interpro

IPR031657   REPA_OB_2        + More
IPR013955   Rep_factor-A_C       
IPR004591   Rfa1       
IPR004365   NA-bd_OB_tRNA       
IPR012340   NA-bd_OB-fold       
IPR007199   Rep_factor-A_N       

SUPFAM

SSF50249   SSF50249       

CDD

cd04476  RPA1_DBD_C
cd04477  RPA1N

ProteinModelPortal

Q5TLD3   H9J7F6   A0A3S2TPC1   A0A1E1W3W0   A0A2A4J7W4   A0A2H1WY01   + More
A0A0N1PEW2   A0A212EIA5   A0A194PNA2   A0A067RKT9   A0A2Z5TR94   A0A2P8Y1D6   A0A2J7Q8Z5   B0W5Z1   A0A1B6CV81   A0A1Q3F4P1   A0A1S4FXA4   Q16KY9   A0A182QCD7   A0A182N5G1   Q7PXI6   A0A182HKU4   A0A182U3F6   U5EWX6   A0A182X9W4   A0A182JRW5   W5JEE8   A0A023EVB3   A0A182VRB5   A0A182RPL3   A0A182Y582   A0A182MBH5   A0A182FP30   A0A182P2E3   A0A182JAC3   A0A0K8VQU9   A0A034WC14   W8BK99   A0A0A9YUP1   A0A146L4T4   A0A023F2L9   A0A224XK33   A0A1B0G3S9   A0A069DVV0   A0A084VW44   A0A0P4VM31   A0A1B0A0G5   A0A2S2PYK9   A0A1B6HZS7   A0A1B0BMK5   A0A1I8MNT3   A0A0V0G459   A0A1A9Y0Z1   A0A164NPL0   B4M169   A0A2A3ERK1   A0A0P6BZA4   B4PT26   A0A1B6MN54   A0A0P6EY13   B3NZV7   A0A0M4F3V6   B4HLN6   B4QYN5   Q24492   A0A2H8TNF8   A0A0P6I084   A0A2R7W1L9   J9K097   T1HTA7   A0A154PH53   A0A1A9VAH6   B4K953   A0A1I8QBZ9   A0A1Y1NLC2   A0A232EXI6   A0A087ZZR0   V9IGY9   A0A0N0U3V3   B4JF42   A0A3B0JPG3   A0A0P5TR09   Q298Q4   B4G4G0   B3MSV9   A0A1A9W3T9   B4NAK2   A0A2S2PBM4   A0A1B0CNK6   V5IEU7   A0A2P6KVW8   A0A1W4VVD2   A0A131XU94   A0A0L7R1H7   A0A3B4ZVY3   R7V2A0   A0A336LJ18   A0A293LLH6   D7EJW6   A0A2R5LE87  

PDB

4GOP     E-value=1.25606e-84,     Score=800

KEGG

692484    K07466 replication factor A1 | (RefSeq) Rpa1, BmRPA1; replication protein A1

PATHWAY

03030    DNA replication - Bombyx mori (domestic silkworm)
03420    Nucleotide excision repair - Bombyx mori (domestic silkworm)
03430    Mismatch repair - Bombyx mori (domestic silkworm)
03440    Homologous recombination - Bombyx mori (domestic silkworm)
03460    Fanconi anemia pathway - Bombyx mori (domestic silkworm)

GO

GO:0006310   GO:0046872   GO:0003677   GO:0006281   GO:0005634   GO:0006260   GO:0051321   GO:0043565   GO:0006268   GO:0003697   GO:0043047   GO:0000723   GO:0000724   GO:0006261   GO:0005662   GO:0007004   GO:0000784   GO:0003684   GO:0006289   GO:0007507   GO:0003676   GO:0006418   GO:0009058   GO:0016742   GO:0003824  

Subcellular location

Nucleus  

Length:

598

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.0659100000000001

Exp number, first 60 AAs:

0.03129

Total prob of N-in:

0.00299

outside

1  -  598

 

 

Pi

180.394627

Theta

169.56177

Tajima's D

-1.081252

CLR

143.425559

CSRT

0.124293785310734

Interpretation

Uncertain