Gene

KWMTBOMO04467

Pre Gene Modal

BGIBMGA005373

Annotation

PREDICTED:_cationic_amino_acid_transporter_4_[Amyelois_transitella]

Location

Bomo_Chr8(-):7129422-7134414      View in EpiBrowser

Full name

Lipoyl synthase, mitochondrial      

Alternative Name

Lipoate synthase
Lipoic acid synthase

Location in the cell

PlasmaMembrane   Reliability : 4.946

CDS

ATGCCGGGCGCAAGGCACAAGATCCTTGGCCATGTGCTATCCGGCTTCTGTCATAAAATGAATCGATGCAAGCCACTACACGGGGATTCCATGGATACTCCCCTAAATAGATGTCTAACCACTTTTGATATAACATTACTCGGCGTGGGTCACATGGTGGGTGCAGGGATATATGTACTTACCGGAACTGTTGCAAAATCAATGGCTGGTCCTTCGACTGCACTAAGTTTCCTTCTGGCGGGGATAACTTCGACTCTCGCTGCCCTTTGTTATGCTGAATTCGGGACTCGAATACCACGAGCGGGTAGCGCGTACGCTTATACCTATGTCAGTATTGGTGAATTTTGGGCCTTTATTATCGGATGGAATATTGTTTTGGAATATATGATTGGTGCTGCTTCAGTGGCGAGAGCTTGGTCAGGATATTTGGATTCTATATTACATGGGGCTATCAGTAATGCCACAGTAGCGTTAACTGGTGAACTTCATGAAACACTTTTAAGCAGATATCCTGATATTCTAGCGTTTCTCATATGTCTAGCAGCGTCCTTGATACTCGCTGCGGGTGTTAAAACATCAGCCTATATCAATAACGGCCTGACTATTTTAAATCTTTTGGTCATCTCTTTAGTAATCTTTTTGGGTTTTTATTATGCTGATATCAGCAACTGGTCAGAGAAAAATGGTGGTTTTATGCCGTTTGGTTTTAGTGGTGTGTTAGCAGGAGCTGCTACTTGTTTTTACGCTTTTGTAGGGTTTGATAGTATATCTGCTTCCAGTGAAGAAGCCAAAGATCCTTCTCGCTCTATTCCTGTTGCAACAGTATTATCAATGGCTGTAGTGGCTTTTGGTTATATTTTGGTCGCGATGGCATTAACTTTAATGGTTCCATATAATACAATTAATCCAGATGCTGCGCTTCCAGCTGCTCTTGGTGCAGTCCACGCAGACTGGGCAAAATATGCAGTTGCTGTGGGTGCAGTATGTGGAATGACAACAACTTTACTTGGATCCATGTTTTCACTACCACGATGTCTATATGCTATGTCTGCCGACGGTTTGCTTTTTGGGTTCTTCAGCGACATTAGTAACAAGGCCCAAATTCCTGTTGCTAATTTAGCTATTTCTGGTTTATCGTCTGCTTTCATAGCTCTTCTTTTTGATTTGGAAAAACTTGTAGAATTTATGTCTATAGGCACATTGTTAGCCTACACGATTGTTAGTGCCGCCGTAATAATATTGAGATATCGTCCAACTCCAACAACAGAAGATAAAGGTTTTGTTGTCCCACAGTTAGATTCACCTTGTGATCGAGAGGATAGTTCAGCAACTGGAACGCCGGCAACGGATGGCGGATCTTCTTCTTCTGAGATGTTTGAAACATTAACGGTCGGTCGTCTACGGCCTCAATTCGCATGGCTAGAACCTCTTGTTGGTGGTAGAGCCCCTGGTGCTGCTGTGACATGTTCTGTGTATACATTTACGATTGCTACTGCGGCTCTGTGTGCTCACAACCATTTTCTCGTCGAACCTGCTGGATTATGGGTGTTATTGCCGGACTTCGTTCTTATTTTCATCATCATCTCGTGTTTGGTGATAATCTGGGCTCACCAACAAAGTCCGACTCGTTTACCGTTCCGTGTACCTTGGGTACCACTGTTACCTGCTGCAAGTGTGATGCTCAACGTAGAACTTATGATTAATCTGAATGCTCTAACTTGGGCACGCTTTGCTATGTGGATGACTTTCGGTCTTCTTGTATATTTCCTATACGGTATCCATCACAGCAAGCTGGGAGAGGGTGTCGCCGGTTTGCTATCCAGCGGTGGAAATAACAGCGATTGGGGTGCAGTAGAAAAGACGAGTTCACGACGTATTGGCCGCTTTGGTCGGAGCAGTAAAGGAGATGACCGCAAACCCATCATTTGTGAAGACGAACTCTCTAGAAGAGAACCTTGA

Protein

MPGARHKILGHVLSGFCHKMNRCKPLHGDSMDTPLNRCLTTFDITLLGVGHMVGAGIYVLTGTVAKSMAGPSTALSFLLAGITSTLAALCYAEFGTRIPRAGSAYAYTYVSIGEFWAFIIGWNIVLEYMIGAASVARAWSGYLDSILHGAISNATVALTGELHETLLSRYPDILAFLICLAASLILAAGVKTSAYINNGLTILNLLVISLVIFLGFYYADISNWSEKNGGFMPFGFSGVLAGAATCFYAFVGFDSISASSEEAKDPSRSIPVATVLSMAVVAFGYILVAMALTLMVPYNTINPDAALPAALGAVHADWAKYAVAVGAVCGMTTTLLGSMFSLPRCLYAMSADGLLFGFFSDISNKAQIPVANLAISGLSSAFIALLFDLEKLVEFMSIGTLLAYTIVSAAVIILRYRPTPTTEDKGFVVPQLDSPCDREDSSATGTPATDGGSSSSEMFETLTVGRLRPQFAWLEPLVGGRAPGAAVTCSVYTFTIATAALCAHNHFLVEPAGLWVLLPDFVLIFIIISCLVIIWAHQQSPTRLPFRVPWVPLLPAASVMLNVELMINLNALTWARFAMWMTFGLLVYFLYGIHHSKLGEGVAGLLSSGGNNSDWGAVEKTSSRRIGRFGRSSKGDDRKPIICEDELSRREP

Description

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.

Catalytic Activity

[[Fe-S] cluster scaffold protein carrying a second [4Fe-4S](2+) cluster] + 4 H(+) + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + 2 5'-deoxyadenosine + [[Fe-S] cluster scaffold protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 L-methionine + 2 reduced [2Fe-2S]-[ferredoxin]

Cofactor

[4Fe-4S] cluster

Similarity

Belongs to the radical SAM superfamily. Lipoyl synthase family.

Uniprot

H9J781   A0A2H1W7F3   A0A2A4JZH1   A0A212FEP9   A0A194PM86   A0A194QSG4   + More
A0A3S2NI70   A0A0L7LAT9   A0A1B6D6M1   A0A067QSN3   A0A2R7WD22   A0A2J7PHM4   T1I0G7   A0A023F3A9   A0A084VKX1   A0A182JRK1   B0WAU5   A0A182MYD5   A0A182QTQ2   Q7Q8T5   B4KYY5   A0A182JKW0   A0A182LQ24   Q16W21   B4J3M8   A0A182X1V2   A0A182HFR6   A0A182YA31   A0A182P8H5   A0A0M3QWS7   W5JEE3   A0A182G9I6   A0A182S4V6   A0A182SPN1   A0A182URT1   A0A0L0C7Y6   A0A182FUK1   A0A2H8TCN8   A0A336MAD2   A0A1W4WIX7   Q2LYJ7   B4HBV1   A0A3B0K3T6   B4LD69   A0A2A3E9C6   B3M989   B3NIL2   A0A1W4W6S6   V9IDC4   A0A1B0GHL3   Q9VPG2   B4PF89   B4QRY6   Q6NNV9   D6WY56   B4MLJ1   A0A154PG88   K7IU03   A0A0L7R6N6   A0A151WP18   F4X1B7   A0A195DP28   A0A232EP67   A0A151JSU7   A0A164QTX2   A0A0P5SZP7   A0A0N8E7Z0   A0A0N0BKD7   A0A2P8YWU4   A0A182VTB7   Q16N09   A0A195BCN1   A0A088ASR1   A0A1B0DAS8   A0A0P6A948   A0A182U709   A0A0C9R8E4   A0A182MCA8   A0A0P6JV95   A0A1I8N3M4   E2B0W3   A0A1I8PJB1   A0A0T6B709   A0A3L8DAR3   A0A026WWI0   T1IVD2   A0A3B0K1Z5   V9IFJ9   A0A195D727   A0A3Q0J2D8   A0A158NFQ8   A0A2M3Z5B4   A0A2M3Z5F6   A0A0P5B888   A0A0P5W918   A0A0N8A1V7   W5KCS5   A0A1W4YFI1   A0A2D0QXU2   F6SWF3  

EC Number

2.8.1.8

Pubmed

19121390    22118469    26354079    26227816    24845553    25474469    + More
24438588    12364791    17994087    20966253    17510324    25244985    20920257    23761445    26483478    26108605    15632085    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    26109357    26109356    17550304    22936249    18362917    19820115    20075255    21719571    28648823    29403074    25315136    20798317    30249741    24508170    21347285    25329095    20431018   

EMBL

BABH01013671    BABH01013672    BABH01013673    ODYU01006801    SOQ48988.1    NWSH01000327    + More
PCG77415.1    AGBW02008899    OWR52235.1    KQ459599    KPI94437.1    KQ461154    KPJ08427.1    RSAL01000088    RVE48220.1    JTDY01001897    KOB72592.1    GEDC01015975    JAS21323.1    KK853329    KDR08430.1    KK854586    PTY17141.1    NEVH01025135    PNF15825.1    ACPB03016100    GBBI01002812    JAC15900.1    ATLV01014310    KE524956    KFB38615.1    DS231875    EDS41811.1    AXCN02002053    AAAB01008933    EAA09935.2    CH933809    EDW18877.1    CH477577    EAT38775.1    CH916366    EDV97259.1    APCN01005567    CP012525    ALC44629.1    ADMH02001479    ETN62406.1    JXUM01049229    JXUM01049230    KQ561596    KXJ78053.1    JRES01000760    KNC28543.1    GFXV01000072    MBW11877.1    UFQT01000621    SSX25849.1    CH379069    EAL29865.3    CH479267    EDW39545.1    OUUW01000012    SPP87342.1    CH940647    EDW69950.1    KZ288334    PBC27882.1    CH902618    EDV40073.2    CH954178    EDV52508.1    JR040284    AEY59065.1    AJWK01005316    AE014296    BT099767    AAF51591.1    ACV82466.1    CM000159    EDW95175.1    CM000363    CM002912    EDX11232.1    KMZ00780.1    BT011174    AAR88535.1    KQ971362    EFA08956.2    CH963847    EDW72847.1    KQ434890    KZC10464.1    KQ414646    KOC66411.1    KQ982893    KYQ49558.1    GL888528    EGI59789.1    KQ980662    KYN14670.1    NNAY01002993    OXU20155.1    KQ982011    KYN30675.1    LRGB01002371    KZS08057.1    GDIP01132985    JAL70729.1    GDIQ01156015    GDIQ01153355    GDIQ01052948    JAN41789.1    KQ435700    KOX80454.1    PYGN01000312    PSN48720.1    CH477843    EAT35719.1    KQ976514    KYM82313.1    AJVK01013390    AJVK01013391    GDIP01033145    JAM70570.1    GBYB01004310    JAG74077.1    AXCM01009715    GDIQ01007481    JAN87256.1    GL444701    EFN60666.1    LJIG01009402    KRT83151.1    QOIP01000010    RLU17401.1    KK107079    EZA60168.1    JH431581    SPP87343.1    JR040283    AEY59064.1    KQ976750    KYN08681.1    ADTU01014565    ADTU01014566    GGFM01002952    MBW23703.1    GGFM01002983    MBW23734.1    GDIP01188085    JAJ35317.1    GDIP01089504    JAM14211.1    GDIP01190681    JAJ32721.1    AAMC01008374   

Proteomes

UP000005204    UP000218220    UP000007151    UP000053268    UP000053240    UP000283053    + More
UP000037510    UP000027135    UP000235965    UP000015103    UP000030765    UP000075881    UP000002320    UP000075884    UP000075886    UP000007062    UP000009192    UP000075880    UP000075882    UP000008820    UP000001070    UP000076407    UP000075840    UP000076408    UP000075885    UP000092553    UP000000673    UP000069940    UP000249989    UP000075900    UP000075901    UP000075903    UP000037069    UP000069272    UP000192223    UP000001819    UP000008744    UP000268350    UP000008792    UP000242457    UP000007801    UP000008711    UP000192221    UP000092461    UP000000803    UP000002282    UP000000304    UP000007266    UP000007798    UP000076502    UP000002358    UP000053825    UP000075809    UP000007755    UP000078492    UP000215335    UP000078541    UP000076858    UP000053105    UP000245037    UP000075920    UP000078540    UP000005203    UP000092462    UP000075902    UP000075883    UP000095301    UP000000311    UP000095300    UP000279307    UP000053097    UP000078542    UP000079169    UP000005205    UP000018467    UP000192224    UP000221080    UP000008143   

PRIDE

H9J781    Q9VPG2    Q6NNV9   

Pfam

PF13906   AA_permease_C        + More
PF13520   AA_permease_2
PF13855   LRR_8
PF16881   LIAS_N
PF04055   Radical_SAM
PF09766   FmiP_Thoc5

Interpro

IPR032675   LRR_dom_sf        + More
IPR029485   CAT_C       
IPR001611   Leu-rich_rpt       
IPR003591   Leu-rich_rpt_typical-subtyp       
IPR002293   AA/rel_permease1       
IPR003698   Lipoyl_synth       
IPR007197   rSAM       
IPR031691   LIAS_N       
IPR006638   Elp3/MiaB/NifB       
IPR013785   Aldolase_TIM       
IPR019163   THO_Thoc5       

Gene 3D

3.80.10.10     3.20.20.70    

ProteinModelPortal

H9J781   A0A2H1W7F3   A0A2A4JZH1   A0A212FEP9   A0A194PM86   A0A194QSG4   + More
A0A3S2NI70   A0A0L7LAT9   A0A1B6D6M1   A0A067QSN3   A0A2R7WD22   A0A2J7PHM4   T1I0G7   A0A023F3A9   A0A084VKX1   A0A182JRK1   B0WAU5   A0A182MYD5   A0A182QTQ2   Q7Q8T5   B4KYY5   A0A182JKW0   A0A182LQ24   Q16W21   B4J3M8   A0A182X1V2   A0A182HFR6   A0A182YA31   A0A182P8H5   A0A0M3QWS7   W5JEE3   A0A182G9I6   A0A182S4V6   A0A182SPN1   A0A182URT1   A0A0L0C7Y6   A0A182FUK1   A0A2H8TCN8   A0A336MAD2   A0A1W4WIX7   Q2LYJ7   B4HBV1   A0A3B0K3T6   B4LD69   A0A2A3E9C6   B3M989   B3NIL2   A0A1W4W6S6   V9IDC4   A0A1B0GHL3   Q9VPG2   B4PF89   B4QRY6   Q6NNV9   D6WY56   B4MLJ1   A0A154PG88   K7IU03   A0A0L7R6N6   A0A151WP18   F4X1B7   A0A195DP28   A0A232EP67   A0A151JSU7   A0A164QTX2   A0A0P5SZP7   A0A0N8E7Z0   A0A0N0BKD7   A0A2P8YWU4   A0A182VTB7   Q16N09   A0A195BCN1   A0A088ASR1   A0A1B0DAS8   A0A0P6A948   A0A182U709   A0A0C9R8E4   A0A182MCA8   A0A0P6JV95   A0A1I8N3M4   E2B0W3   A0A1I8PJB1   A0A0T6B709   A0A3L8DAR3   A0A026WWI0   T1IVD2   A0A3B0K1Z5   V9IFJ9   A0A195D727   A0A3Q0J2D8   A0A158NFQ8   A0A2M3Z5B4   A0A2M3Z5F6   A0A0P5B888   A0A0P5W918   A0A0N8A1V7   W5KCS5   A0A1W4YFI1   A0A2D0QXU2   F6SWF3  

PDB

6F34     E-value=1.33463e-64,     Score=627

KEGG

101745401    K13866 solute carrier family 7 (cationic amino acid transporter), member 4 | (RefSeq) cationic amino

GO

GO:0016021   GO:0022857   GO:0046872   GO:0016992   GO:0005739   GO:0051539   GO:0009249   GO:0016020   GO:0003333   GO:0015171   GO:0006281   GO:0008270   GO:0004812   GO:0006418   GO:0009058   GO:0016742   GO:0003824   GO:0055085  

Subcellular location

Mitochondrion  

Length:

652

Number of predicted TMHs:

14

Exp number of AAs in TMHs:

297.93206

Exp number, first 60 AAs:

18.40728

Total prob of N-in:

0.69655

POSSIBLE N-term signal

sequence

inside

1  -  45

TMhelix

46  -  65

outside

66  -  68

TMhelix

69  -  91

inside

92  -  103

TMhelix

104  -  126

outside

127  -  172

TMhelix

173  -  190

inside

191  -  196

TMhelix

197  -  219

outside

220  -  228

TMhelix

229  -  251

inside

252  -  271

TMhelix

272  -  294

outside

295  -  320

TMhelix

321  -  343

inside

344  -  367

TMhelix

368  -  387

outside

388  -  391

TMhelix

392  -  414

inside

415  -  476

TMhelix

477  -  499

outside

500  -  513

TMhelix

514  -  536

inside

537  -  547

TMhelix

548  -  567

outside

568  -  571

TMhelix

572  -  594

inside

595  -  652

 

 

Pi

208.586727

Theta

210.558069

Tajima's D

0.023265

CLR

0.720468

CSRT

0.375331233438328

Interpretation

Uncertain