SGID Silkworm Genome Informatics Database
Gene
KWMTBOMO04438
Pre Gene Modal
BGIBMGA005382
Annotation
DNA_cytosine-5_methyltransferase_[Bombyx_mori]
Full name
DNA (cytosine-5)-methyltransferase       + More
Cytosine-specific methyltransferase      
DNA (cytosine-5)-methyltransferase PliMCI      
DNA (cytosine-5)-methyltransferase 1      
Alternative Name
DNA methyltransferase PliMCI
Dnmt1
MCMT
DNA methyltransferase GgaI
Location in the cell
Nuclear   Reliability : 3.783
 

Sequence

CDS
ATGCCCACTTCAACTATTACTTGCAACAGTATAACACAAGTGTGCATGGATAAGATACTTGATGGGGATGAAATAATCAAAGAAAACTCTAAGAGAAAAAGGAGCTGTGAAGAAATTGTTTCTGCCAATAATAAACGATCAAAAACAGAAGACAATGATGGACATCAAATTTCACAAGATTCTACAAGCAATGACTCTATTGAAAATCTTAACATTCCAGCAAAAAATACTAGTCTTATTGTTTCTGAAAACATCAATAATGTATATAAAAATTATGACGAGGTTGAAAATAAACCAGTATATAAAAATGGTGACCCTATCGTTGTTGAAATAGATAACAAGGATAAGCCTGGATCAGTTAACAACAATCATGACATGATTGATGATTCAGAGACCATATCTAATATTAAGAATAGCAATAATATTATCCCTGATACAGAGAAATGTAACATATGTGGCCAATTTTTAAATAATTCTGACTTAATTTATTACCAAGGTCACCCACAAGATGCTGTTGAAGAATATATTGCATTGACTAATGACAAACTTGTTTTGAGCTCTGGGGAAGATGGAGACATAATGGAACGACCCCAAACAAATATAACTGGCTTTACAATATTTGATGAGCAAGGACATTTGTGTCCAATTGATGGTGGTTTGGTAGAAAACGATGTTCGAATTTATATGTCTGGATATTTAAAGTCAATTTGCTCTGATTCATCAGAGATTGACGAAGAATCCATTCCAGTTAAAGATGTAGGTCCTATAATAGAATGGTTCATACATGGTTTCGATGGTGGAGATAGGAACTGTATAACTTTATCAACGGAATTTGGGGAATATAACTTGCTCAAACCTAGTGAAGCTTATACGCCCCTAATGAATAATTTATATGAAAAAATATGGCTTAGCAAAGTAGTCGTCGAATTCTTAGAAGAATATCACTACTTGCAACCGAGCTATGAAGATTTACTTGAAGTTGTAAGAGATTTTTCCATTCCTGAACTAAATAATAAAAAGATGACAGAAGAAATGCTACATAAACATGCCCAGTTTGTTTGTGATCAAGTAGTTAGCTTAGAAATAGAGGAAGATGATGAACCATTAATAACATTACCTTGTATGAGAGAACTTATCAAATTAATGGGCATAAAATTCGGTAAACGAAAAATACGTACCCAGATACAATACAAAAAAACAGATAAGAAAGCATGGACAAAAGCTACAACAACTCCACTTGTAAGAAAAACATTTGAAAGTTTTTTCTCAAATCAGTTAGACAAAACCAATCACGAACTGGTCTTACGAAGAAAACGATGCGGGGTTTGTGAAGCCTGTCAATTGCCTGATTGCGGCGAATGTAATGCATGTCGTGCCATGGCTAAATTCGGTGGTCATGGAAGAACTAAGAAAGCATGCGTCCGTAGATTATGTCCTAATATGGCAGTAGAACAGGCAGAAGATTCTGATCCCGATGATGAAGATGAGTATCAGCAAATATCTGAGAAAAAACAAGACAAAATAGATGATGCAGTACCAGTCAAGCTCACTGGGTCAAATAGTAAAAATTTAAAATGGATTGGTGAGCCTGTTAAAGCTGATGCCACAAAAATATATTACGAAAAAGTTGAAATTGATGGTGCTGAGCTGTGTAATGGTGACTTTGTCATGATTGAAACTTCCCAAACAAACATTCCTACACTGGTCGCTAAAGTTGTATACATGTGGAAAGAAATCCATAACCCGAAATCAGGCTATTTTCACGGAGAAGTCTTCATACGAGCCTCGGACACAGTTTTAGGAGAAGTGAGTGACCCAAGAGAAGTATTTTTGGGCGACAGATGTTGCCATGGTGCTCCGCTTTCTTCCATATTAAGAAAAGCGAATATAGAAAGGAAAGAGACGTCAGCAGATTGGTTTAAACTTGGCGGGAAAGAAGTAGACGACGAACATTTCGAGGATGATGGAAGAACTTATTTTTATAGCAAGTATTACGACAGGTTCACGTCGCGTTTTGAAGATTTACCTCCAGATCCAGCGTGTCCAAATGCTTTAAGAAAGCACAGATTCTGCCCATCTTGTGAACGTAAAACAAAACGAGATGCAAGAAACATTCCTAAAGTTTTTGAAAAATTGATTGTGAAATCCGAAATTGTTTCAGAACAAAACAGGTCGGAATGGTCATATGTAAAATGGCAAGACTTTGACTATAAGAAGGGTTGTGGTGTTTTCCTAAAACCGGGAACCTTTAAACTAAAAAATAGTATGACAAAAGCGAACACTGTTGCCAAACCGCGGTTTGAAAAAGTCGATGAAACTATTTATCCTGAATATTATAGGAAGAACGATTCTAATACGCGTGGGTCTAATATTGATACCGGGGAACCTTTTTGTGTGGGTTACATAGCTGCCGTCACTGCCGCGAGTGAGGGACCTCTAGTTGTACCGCAAGATATTTACCTGAAAGTGAATGTGCTGCTTAGACCTGAAAACACGAGCAGTAAATTTCCGCAGCACGAGGACACTAATGTTTTATATTGGACAACTGAAATAAGGGAGATTCCATTTTCGACCGTCGTTGGGCATTGTCATTTAATTTATGAACAAAATGTTCCCCAAAATATTTCACTTCAAGAATGGTTAGGAAATGATCCGTGCAGATTCTACTTCAGAATGGCGTATTGTAAGTCCACGGGAGAGTTCACGGATCTCCCGCAGAATGCCATCAGTGTTGGAAGAACAGATCGTACTAAAGATAAAGGGAAGGGGAAGGGAAAATCTACAAAAACTATTGAAACGGTTCCCGCTAAAGTTGTAGAGGAGAAAATTAGACCGTTGCGAACTTTAGACGTGTTTGCCGGTTGTGGGGGCCTATCAGAAGGCTTGCATCAAGCTGGAGTTGCAGAATGCAAGTGGGCTATTGAAAACGTGGAAGCTGCTTCTCATGCTTATTCATTAAATAATAAAAGTTGTATTGTATTTAATGAAGACTGTAATGCACTTTTGAAAACTGTAATGTCTGGTGCTAAGCACAGCGCGAATGGACTGCGGCTCCCCATGCAAGGAGAAGTGGAGCTTTTATGTGGCGGGCCACCATGTCAAGGCTTCTCCGGAATGAATCGTTTTAATTCAAGAGAGTATTCAAACTTCAAAAACTCATTAGTTGCATCGTATTTATCGTTTTGTGATTATTACAGACCTAAATATTTTATACTGGAAAACGTTCGTAACTTTGTGGCCTTTAAAAAAGGTATGGTTTTGAAACTCACTCTGCGAGCTTTGTTGGACATGGGATACCAGTGTACCTTTGGTATCTTACAAGCGGGTAACTACGGAGTTCCCCAAACTAGACGAAGGTTGATCATATTGGCGGCAGCGCCGGGTTACAATCTTCCGTTCTACCCAGAACCCACGCACGTCTTCAGTAGACGTGCTTGTACATTAACAACGACGATTGACGGCAAAAGGTTCTCGACTAATATTCACTGGGACGAGTCGGCTCCAAAAAGAACGTGCACCATTCAAGACGCCATGAGTGATTTGCCTCAAATATGTAATGGTGCTAATAAAATAGAAATCGAATATGGCTCCATGCCAGAAAGTCATTTCCAGAGATTAGTGCGAAGCAACGATGAGAATTCTAAACTACGAGATCACATCTGTAAAAACATGGCGCCATTGATTCAAGCTAGGATCAGCAGGATACCTACAACACCGGGCTCCGACTGGAGAGATCTACCCAACATATCGGTTACTCTCTCCGATGGCACTAAGTGCAAGGTTCTACAGTATCGCTACGACGACAAGCGCAATGGGCGGTCGAGCAGCGGCGCAGTGCGCGGCGTGTGCGCGTGCGCGAGCGGCCGCGCCTGCTCGCCGCTGGACAAGCAGGAGAACACGCTCATCCCGTGGTGTCTGCCGCACACCGGCAACAGGCACAACCACTGGGCCGGGCTCTACGGTCGGCTATCGTGGGGCGGGTACTTCAGCACGACAGTGACGGATCCCGAACCGATGGGCAAGCAAGGCCGGGTGCTCCACCCCGACCAGCACCGAGTCGTCTCCGTGCGAGAGTGCGCGCGCTCGCAGGGCTTCCCTGATACGTATCTCTTCGCAGGCTCCGTACAGGACAAACATCGACAGATCGGAAATGCAGTTCCACCACCTCTTGGCGCCGCTCTGGGTAGAGAAATAAAGAAAGCGCTTACTCTCAGCCTGACCACATCATGA
Protein
MPTSTITCNSITQVCMDKILDGDEIIKENSKRKRSCEEIVSANNKRSKTEDNDGHQISQDSTSNDSIENLNIPAKNTSLIVSENINNVYKNYDEVENKPVYKNGDPIVVEIDNKDKPGSVNNNHDMIDDSETISNIKNSNNIIPDTEKCNICGQFLNNSDLIYYQGHPQDAVEEYIALTNDKLVLSSGEDGDIMERPQTNITGFTIFDEQGHLCPIDGGLVENDVRIYMSGYLKSICSDSSEIDEESIPVKDVGPIIEWFIHGFDGGDRNCITLSTEFGEYNLLKPSEAYTPLMNNLYEKIWLSKVVVEFLEEYHYLQPSYEDLLEVVRDFSIPELNNKKMTEEMLHKHAQFVCDQVVSLEIEEDDEPLITLPCMRELIKLMGIKFGKRKIRTQIQYKKTDKKAWTKATTTPLVRKTFESFFSNQLDKTNHELVLRRKRCGVCEACQLPDCGECNACRAMAKFGGHGRTKKACVRRLCPNMAVEQAEDSDPDDEDEYQQISEKKQDKIDDAVPVKLTGSNSKNLKWIGEPVKADATKIYYEKVEIDGAELCNGDFVMIETSQTNIPTLVAKVVYMWKEIHNPKSGYFHGEVFIRASDTVLGEVSDPREVFLGDRCCHGAPLSSILRKANIERKETSADWFKLGGKEVDDEHFEDDGRTYFYSKYYDRFTSRFEDLPPDPACPNALRKHRFCPSCERKTKRDARNIPKVFEKLIVKSEIVSEQNRSEWSYVKWQDFDYKKGCGVFLKPGTFKLKNSMTKANTVAKPRFEKVDETIYPEYYRKNDSNTRGSNIDTGEPFCVGYIAAVTAASEGPLVVPQDIYLKVNVLLRPENTSSKFPQHEDTNVLYWTTEIREIPFSTVVGHCHLIYEQNVPQNISLQEWLGNDPCRFYFRMAYCKSTGEFTDLPQNAISVGRTDRTKDKGKGKGKSTKTIETVPAKVVEEKIRPLRTLDVFAGCGGLSEGLHQAGVAECKWAIENVEAASHAYSLNNKSCIVFNEDCNALLKTVMSGAKHSANGLRLPMQGEVELLCGGPPCQGFSGMNRFNSREYSNFKNSLVASYLSFCDYYRPKYFILENVRNFVAFKKGMVLKLTLRALLDMGYQCTFGILQAGNYGVPQTRRRLIILAAAPGYNLPFYPEPTHVFSRRACTLTTTIDGKRFSTNIHWDESAPKRTCTIQDAMSDLPQICNGANKIEIEYGSMPESHFQRLVRSNDENSKLRDHICKNMAPLIQARISRIPTTPGSDWRDLPNISVTLSDGTKCKVLQYRYDDKRNGRSSSGAVRGVCACASGRACSPLDKQENTLIPWCLPHTGNRHNHWAGLYGRLSWGGYFSTTVTDPEPMGKQGRVLHPDQHRVVSVRECARSQGFPDTYLFAGSVQDKHRQIGNAVPPPLGAALGREIKKALTLSLTTS

Summary

Description
Methylates CpG residues. Preferentially methylates hemimethylated DNA. It is responsible for maintaining methylation patterns established in development. Mediates transcriptional repression by direct binding to HDAC2. Plays a role in promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes. Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing.
Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells. Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing. Promotes tumor growth.
Catalytic Activity
a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = a 5-methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine
Subunit
Homodimer (By similarity). Interacts with PCNA (PubMed:9302295).
Homodimer. Forms a stable complex with E2F1, BB1 and HDAC1. Forms a complex with DMAP1 and HDAC2, with direct interaction. Interacts with the PRC2/EED-EZH2 complex. Probably part of a corepressor complex containing ZNF304, TRIM28, SETDB1 and DNMT1. Interacts with UHRF1; promoting its recruitment to hemimethylated DNA. Interacts with USP7, promoting its deubiquitination. Interacts with BAZ2A/TIP5. Interacts with PCNA. Interacts with MBD2 and MBD3. Interacts with DNMT3A and DNMT3B. Interacts with UBC9. Interacts with HDAC1. Interacts with CSNK1D.
Similarity
Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.
Keywords
DNA-binding   Metal-binding   Methyltransferase   Nucleus   Repeat   S-adenosyl-L-methionine   Transferase   Zinc   Zinc-finger   Complete proteome   Phosphoprotein   Reference proteome   Repressor   Transcription   Transcription regulation   Acetylation   Activator   Chromatin regulator   Isopeptide bond   Methylation   Ubl conjugation  
Feature
chain  DNA (cytosine-5)-methyltransferase PliMCI
EC Number
2.1.1.37
EMBL
AB194008    BAD67189.1    LC010238    BAP86925.1    BABH01013633    ODYU01010002    + More
SOQ54793.1    JTDY01002883    KOB70543.1    MF347704    AWI66421.1    AGBW02008764    OWR52605.1    KQ460397    KPJ15240.1    JTDY01003135    KOB70090.1    KQ435012    KZC13768.1    KQ434870    KZC09572.1    KQ414585    KOC70520.1    FX985462    BBC27273.1    KZ288347    PBC27578.1    KK107061    EZA61363.1    GEDC01029530    JAS07768.1    GL453161    EFN76367.1    NNAY01002670    OXU20811.1    KF294265    AHZ08393.1    GU907553    ASM42030.1    KZ288444    PBC25685.1    GEBQ01032237    JAT07740.1    NEVH01013194    PNF30164.1    KQ435727    KOX77963.1    RCHS01003956    RMX38664.1    KQ979074    KYN22712.1    GL888237    EGI64171.1    LSMT01000002    PFX34776.1    ADTU01002818    KQ976565    KYM80462.1    KQ981215    KYN44594.1    KQ761467    OAD57514.1    GBBI01003378    JAC15334.1    KQ422062    KOF75587.1    KQ982706    KYQ51853.1    KX064246    ANN46487.1    GFTR01008788    JAW07638.1    KC210883    AGK72242.1    JX027277    AFR91943.1    GG666566    EEN54323.1    AAGJ04051615    AAGJ04051616    AAGJ04051617    Z50183    AJ495767    AJ495845    AJ495846    AJ495847    AJ495848    AJ495849    AJ495850    AJ495851    AJ495852    AJ495853    AJ495854    AJ495855    AJ496187    CAD42182.3    AKHW03000333    KYO47915.1    GEZM01014671    JAV92043.1    AADN05000499    D43920    JT410353    AHH39024.1    PYGN01000608    PSN43414.1    AAMC01032281    LSYS01008266    OPJ69161.1    AEMK02000012    AYCK01008264    AY244709    AY173048    BC114063   
Pfam
PF12047   DNMT1-RFD        + More
PF00145   DNA_methylase
PF02008   zf-CXXC
PF01426   BAH
PF06464   DMAP_binding
Interpro
IPR029063   SAM-dependent_MTases        + More
IPR031303   C5_meth_CS       
IPR001525   C5_MeTfrase       
IPR018117   C5_DNA_meth_AS       
IPR001025   BAH_dom       
IPR017198   DNMT1-like       
IPR022702   Cytosine_MeTrfase1_RFD       
IPR002857   Znf_CXXC       
IPR010506   DMAP1-bd       
SUPFAM
SSF53335   SSF53335       
PDB
4WXX     E-value=0,     Score=2896

Ontologies

Topology

Subcellular location
Nucleus  
Length:
1409
Number of predicted TMHs:
0
Exp number of AAs in TMHs:
0.01602
Exp number, first 60 AAs:
0
Total prob of N-in:
0.00000
outside
1  -  1409
 
 

Population Genetic Test Statistics

Pi
182.670958
Theta
207.13532
Tajima's D
-2.346409
CLR
1.965968
CSRT
0.00264986750662467
Interpretation
Uncertain
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