SGID Silkworm Genome Informatics Database
Gene
KWMTBOMO04397
Pre Gene Modal
BGIBMGA005239
Annotation
PREDICTED:_alpha-amylase_4N-like_[Papilio_polytes]
Full name
Alpha-amylase       + More
Alpha-amylase 4N      
Location in the cell
Cytoplasmic   Reliability : 1.402 Extracellular   Reliability : 2.073
 

Sequence

CDS
ATGCCTCAGTTGCGATTGCTTCTCGTGTGTTTAATCTTGGGTTTCGTGCTATGCAATCATCATAAAAGGACGAACCAAGATCTCAACCGGTCGACAATTGTGCATCTCTTCGAATGGAAATGGAAGGATATTGCTGATGAATGCGAGAGATTCCTTGCGCCGAAAGGCTTCGGTGGAGTTCAAGTATCTCCACCCTCGGAGAATGTTATCATTCGTTTATCAGACGGTACACGACCTTGGTATGAACGTTACCAAGTAATGTCCTACAAGTTGATCACCAGATCAGGAAATCAAGATGAATTTTTGAACATGACCCGAAGATGTAATGATGTTGGGATAAGAATTTACGCTGACGTGGTAATTAATCATATGACGAGTGACAATAGGGAATCTGTTGGTACAGGGGGTAGTACAGCCAACTATAAAGAGTACAGCTACCCCGCTGTGCCGTACACGAGAGACCACTTCCATCATCCGTCCTGCTCTATTAATAATTATAATGATGCTGCACAGGTAAGGGCCTGCGATTTGGTTGGATTGAAGGACTTGAATCACAACTTGCCGTATGTTCGTGACAAAATCGTTGAATACCTAAACAAGCTTATTGCATTAGGAGTTGCCGGATTCAGAGTGGATGCAGCAAAACATATGTGGCCACACGACCTCAAAGAGATGTACAATAGACTTAATAATCTCAATGTAGATTTTGGCTTCCTACCTAATACAAAACCGTACATTTACCAAGAAGTAATTTATAGAGGGAATGAGCCTATCCAACATACTGAATACACACCCTTTGGTGACGTCACAGAATTTCGGGTCGGATACGAGTTAAAACCAGTTTTTGAGGGTAAAAATCCATTGAAATGGTTGAAGTCCTGGGGCGAGAATTGGAACTTATCACCCAGTGACCGCGTTGTTGTATTCATTGACAATCACGACACGCAGAGGTCGAATGAAGTTTTGACGTACAAAAATGCCAAGTCTTATAAGGCAGCTATAGCGTTTACACTGGCACACCCATATGGCGAACCTCGCGTGATGAGCGGTTATTTCTTCGACAGAACCGACCAAGGTCCACCAAGCGACGGTAACGAGGAAATTTTATCTCCAATCATAAATGATGACGACACCTGCGGCAATGGCTGGGTTTGCGAGCATCGTTGGCGACAGATCTATCAAATGGTCGCATTTAGAAATGCCGTCCGAGATACTAGAGTTGAAAATTGGTGGGACAACGGTCTTAATCAGATAGCTTTCAGTCGAGGAAATAAAGGCTTTATCGCTATTAACGCCGAGGGTCGAGATTTGGATGTTATTTTACAGACTGGTCTTCCATCTGGTACTTATTGCGATGTTATAAGCGGGAAAATTCAAGGCCAAGAATGTTCCGGAAGAAAAATAACAGTTGGCAGTGATGGTCGTGCGCATATTTATGTATCCAAAGACGGGGAGGACATGCATCTTGCCACACATGTTGGGCCGGAGTCATTACTCCAGATCAAGCAGAAGATATGA
Protein
MPQLRLLLVCLILGFVLCNHHKRTNQDLNRSTIVHLFEWKWKDIADECERFLAPKGFGGVQVSPPSENVIIRLSDGTRPWYERYQVMSYKLITRSGNQDEFLNMTRRCNDVGIRIYADVVINHMTSDNRESVGTGGSTANYKEYSYPAVPYTRDHFHHPSCSINNYNDAAQVRACDLVGLKDLNHNLPYVRDKIVEYLNKLIALGVAGFRVDAAKHMWPHDLKEMYNRLNNLNVDFGFLPNTKPYIYQEVIYRGNEPIQHTEYTPFGDVTEFRVGYELKPVFEGKNPLKWLKSWGENWNLSPSDRVVVFIDNHDTQRSNEVLTYKNAKSYKAAIAFTLAHPYGEPRVMSGYFFDRTDQGPPSDGNEEILSPIINDDDTCGNGWVCEHRWRQIYQMVAFRNAVRDTRVENWWDNGLNQIAFSRGNKGFIAINAEGRDLDVILQTGLPSGTYCDVISGKIQGQECSGRKITVGSDGRAHIYVSKDGEDMHLATHVGPESLLQIKQKI

Summary

Catalytic Activity
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.
Cofactor
Ca(2+)
chloride
Subunit
Monomer.
Similarity
Belongs to the glycosyl hydrolase 13 family.
Keywords
Calcium   Carbohydrate metabolism   Chloride   Complete proteome   Disulfide bond   Glycosidase   Hydrolase   Metal-binding   Reference proteome   Signal  
Feature
chain  Alpha-amylase
EC Number
3.2.1.1
EMBL
BABH01028504    RSAL01000030    RVE51685.1    KQ459761    KPJ20139.1    AGBW02008240    + More
OWR53974.1    ODYU01003330    SOQ41941.1    AGBW02007717    OWR54671.1    JRES01000462    KNC31016.1    ODYU01012544    SOQ58927.1    KA644631    AFP59260.1    ODYU01008873    SOQ52846.1    MK075190    AYV99593.1    BABH01018101    BABH01018102    BABH01018103    BABH01018104    NWSH01006767    PCG63259.1    GFDG01000548    JAV18251.1    OWR53975.1    AY333762    AAP97394.1    GDHF01005753    JAI46561.1    U04223    AAA03715.1    NWSH01001047    PCG72932.1    HQ424576    ADP89000.1    GAKP01019473    GAKP01019470    JAC39482.1    AY330289    AAP92665.1    HM357843    AEA76309.1    KQ459580    KPI99290.1    GDHF01027086    JAI25228.1    GAKP01019466    JAC39486.1    EF672102    ABV24963.1    EF600049    EU325552    ABU98614.1    ACB54942.1    AXCN02001626    GAMC01004278    JAC02278.1    GAMC01008204    JAB98351.1    GDHF01018640    JAI33674.1    FJ489868    ACL14798.1    RSAL01000004    RVE54528.1    AF467104    AAO17923.2    AJWK01015266    AJWK01015267    GQ274006    ACT64133.1    BABH01028500    BABH01028501    BABH01028502    MG941008    AYV89929.1    KPI99291.1    GAKP01019468    JAC39484.1    KX398855    ANZ79224.1    AF146757    AAO13691.1    AB078773    BAC06339.1    AB035066    BAA95440.1    NWSH01006246    PCG63558.1    AB078770    BAC06336.1    AB035065    BAA95439.1    AB078765    BAC06331.1    GBXI01001663    JAD12629.1    AB078766    BAC06332.1    KX398852    ANZ79221.1    ATLV01022314    KE525331    KFB47455.1    AB078767    BAC06333.1    AY333761    AAP97393.1    X76241    CAA53820.1    AF280891    AAO13754.1    AB035061    BAA95435.1    AXCM01000750    AB035062    BAA95436.1    AB035060    AB077431    BAA95434.1    BAB91544.1    AB077432    BAB91545.1    AB077436    BAB91549.1    AB035069    BAA95443.1    U53699    AAC35243.1    CH902619    KPU77312.1    EDV35653.1    U53477    U31121    CH933808    EDW08396.1    AB035057    AB035058    BAA95432.1    GANO01001973    JAB57898.1    AB077433    BAB91546.1    AB077391    BAB91504.1    AB077412    AB077413    AB077417    AB077423    AB077424    BAB91525.1    BAB91526.1    BAB91530.1    BAB91536.1    BAB91537.1    AB078769    BAC06335.1    AB078768    BAC06334.1    AB035059    BAA95433.1    AB078771    BAC06337.1    AB077430    BAB91543.1    AB077404    BAB91517.1    AB077388    AB077395    AB077406    AB077407    BAB91501.1    BAB91508.1    BAB91519.1    BAB91520.1    AB035067    AB035068    BAA95442.1    AB078772    BAC06338.1    U53478    AAB69330.1    AB077426    BAB91539.1    KX398853    ANZ79222.1    AB077409    AB077410    AB077411    AB077415    AB077421    AB077422    AB077425    AB077428    AB077429    BAB91522.1    BAB91523.1    BAB91524.1    BAB91528.1    BAB91534.1    BAB91535.1    BAB91538.1    BAB91541.1    BAB91542.1    AAAB01008859    EAA07513.6   
Pfam
PF00128   Alpha-amylase        + More
PF02806   Alpha-amylase_C
PF00155   Aminotran_1_2
Interpro
IPR006047   Glyco_hydro_13_cat_dom        + More
IPR017853   Glycoside_hydrolase_SF       
IPR006046   Alpha_amylase       
IPR031319   A-amylase_C       
IPR006048   A-amylase/branching_C       
IPR013780   Glyco_hydro_b       
IPR015421   PyrdxlP-dep_Trfase_major       
IPR015424   PyrdxlP-dep_Trfase       
IPR004839   Aminotransferase_I/II       
IPR001917   Aminotrans_II_pyridoxalP_BS       
IPR015422   PyrdxlP-dep_Trfase_dom1       
SUPFAM
SSF51445   SSF51445        + More
SSF53383   SSF53383       
PDB
1VIW     E-value=7.36993e-157,     Score=1422

Ontologies

Topology

SignalP
Position:   1 - 18,         Likelihood:  0.967376
 
 
Length:
505
Number of predicted TMHs:
0
Exp number of AAs in TMHs:
0.11163
Exp number, first 60 AAs:
0.10587
Total prob of N-in:
0.00273
outside
1  -  505
 
 

Population Genetic Test Statistics

Pi
240.824248
Theta
168.302705
Tajima's D
1.385034
CLR
0.258557
CSRT
0.75741212939353
Interpretation
Uncertain

Multiple alignment of Orthologues

 
 

Gene Tree

 
 
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