Gene

KWMTBOMO04380

Pre Gene Modal

BGIBMGA005275

Annotation

PREDICTED:_PAB-dependent_poly(A)-specific_ribonuclease_subunit_PAN2_isoform_X2_[Bombyx_mori]

Location

Bomo_Chr8(+):5110208-5132872      View in EpiBrowser

Full name

PAN2-PAN3 deadenylation complex catalytic subunit PAN2      

Alternative Name

PAB1P-dependent poly(A)-specific ribonuclease
Poly(A)-nuclease deadenylation complex subunit 2

Location in the cell

Cytoplasmic   Reliability : 1.3 Nuclear   Reliability : 1.971

CDS

ATGGAATATCATTACTCCGAAATATGTATGCCATCCGATCATCTAACAGATTACACACCCCCTTTCATCATTCCCACGCATGAAGGGGAATATGAAGCACGTAACAGTGTACTCGTGGATGGAGGAGAGAGATTTGGAGTTTCGGCAGTTGTGTTCGATAAACATGAAGAGTTGATATGGATGGGGAATCAGGGTGGTCATGTAACATCCTACTACGGTCCAAATATGCAAAAGTACACATCATTTCAAGTACACGATACTGATGAAGTTAGAGATATTATCACTGTTGAAAAATGTATTTATGTTTTGACCAAAACAACTCTACGCCATCAGTTACGTAGAGGTATACCTAAGCACACGCACAAATCACCAAACATGACAGACATGCAATGCCTGTATCAAGTGAACTCTACAAAATTATTGATGGGCGGACACCAAGACAAACTAATCGATTTGGACCTTAATAAAATGGTTGAAACTATCATTCCAATACAAGAGGAAGGCTGTGCGGTATTAAGAAGCGGTGGTAGCAGTCACGTCGCTTGTGGTAGTGCTAACGGTATAGTGGCATTGCGGGATATGCGTGCTCCTACAAAAGCTGAGCATACATTCAGAGCACACACGGCTTGTCTATCTGATCTGGATATGCAAGGAGATCTGTTGCTCACATGTGGATTTACTCAGGCCGTTGGAGGGGTAGTCGCAGAACCCTACATAGTGGTCTGGGACGTCCGATGTCTACGCGGCAGTAACCCTGGACAAGGAGGTTGGACCATTCATACCACATCACCGCCACTGCTACTTCACTTCCTGCCGGCGTTCTCTGGTAGGGCAGTAGCTGCTTCGGGCGACGGACACGTCAGTTTGTTACACGTGAACGCACCTGAAGAAAGGCAGTCAATTTTTAAGGTAGAGACGCACAGTTCACAGTGCAATGTGATGGACGTTGCGTCGACGAGTCAAGCCCTCGCTTTCGGCGACCAATCAGGACATTTAAATCTTTTCTCACCGACGCAGAATCACGAACCTGTATACAATAATTTTTCAAGGTCAACAGAGTTCGCGGATCCAGTGGAAGGTCTACCTTACGTTCGGTTCAGCGACACCAGGTTCCAGTACTCGTCGGTGCCACTACCTCAGTTGGCGAGCGGCGGGAAATGGTTCAATGAATTGCCACCGGAATTCTTTAGGAAAGTGTATAGAAAACCAAAGCCAGTCGATCCTGAAGTTTTGAAATCGATGAAAATGCAAGGTCCAATAGGATACGCGCCTAATCCGAAAACTTTTAAAAGGAATCAGATGCCGTACCTTGAAGAATCGTTAGAAGAAATGAGTATATCAAAAATAAATGGAAATAAAGTAATAATGCAGCCGATACCGAAACATTATCAGAAGCTCGACATACGATATAATAAGCACGGTCCGAATGAAGAATTAGAGAGTTTCAATAAAACAGGTCAGCCCGGACTGGAAGCGACATTACCGAATTCATACTGCAATGCCATGTTACAGGTTTTGTACTACACGCCGCCGGTCAAAGCCACGTTGCTAGCGCACACGTGCGCCAAAGAGTTCTGTTTGAGCTGCGAATTAGGTTTTCTATTTCGCATGCTGGACACGTCTGGCGGCACCCCTTGTCAGGCCACAAACTTCCTACGCGCGTTTCGTACGGTCCCCGAAGCGGCTGCCCTAGGACTGATACTGCCGGATCGAGCGCCCGACATAAGAGTCGATTTGGTAGCCTTAATACAGAGCTGGAACCGCTTCATCCTGCACCAGATTCATTACGAGATCCTCGAGACCCGTAAGAAGGAGAAGGAGTTAGCGCTGATAGCGAACAGTCCGCCCAAAGCACCACGCGCGTCCAACCCGAAGGAACGTCACTCGCTAATGAACGGACAGTACTCCGAGGAGTGTCAGTATACCGAACTGGAGTTCTTGGGACCCACCGAATTTGATTGTCAAGAGGATGGCGAACTCTGGCCGGGCAGGGATGAAGGCGTCGACGGCGTGGGGCCGTTGCAAAACCACGAAAGTCACGAGCTGAACAACCGACAGGCGCACCGGGAGGAGACCGAGATCTCGCAGCTGTTCGCCATCGGTCGGCATCAGCTCAACCGCTGCTTGAAGTGCAACAAGGAGGAGGGCAGGGAGTCGGTGGTGCTGGCGTGCGCCCTGCAGTACCCGCCGACGGGCGCGGGCGGCGCCGGGGAGGCGAGGGGCGGATTCGTCGAACTCGTGCGCGCGTCGCTGGCGGCGCGGCGGAGCACGCCGGCGTGGTGCGACCACTGCTCGCGGTTCACACCGACCACGCAGCGAGGGCGACTGCTGCGACTACCTCCTATATTGGCGATTAATTGTGGAGGAGTTACGGTCAGAGAGAAAGCGTATTGGGCTAAAGGAGTACAAAAGGAAATGTCCGATTCGAAAAGAGGGGGCAGCGGGAAACCGTGTCGCTACGGCTTACATTGCGCACGACCGGGTTGTCATTTCAAACATCCCGATCGGCCGAATACATCACAAAGTTCTTCCAAGGGCTCGAGCCAGGAGTCGCACTGCATGCTGCCGCTTCAGCTGCTCATCCGGCTTCAGTCAGACGGTGACGTCATCATCAACGACAAGATCGAAACTAATTCAGCGGAGAGTTCGCCGATCAACCGACCGGACACGAGCAGGCACAAAAAGAAGTACGTGACCACGCACCTCGAGGAGGAGTACTCGCTCTCGGCCGCCGTCGTCTGCGTCGAGGACAACCCTAAGAACTTGGTCGCCTACATACACACGGTCCGGGACGGCGAAACTCAATGGTTCATGTACAACGATCTATGTATCGTTCCGGTGAGTACGGACGAGGTGATACATTTCGGCGTGTGGTGGAAAACGCCTTGCGTGCTATTCTACACGGCGACCGGCGGCGGACAGTGCAACGGTGACGTCAGCTCATAG

Protein

MEYHYSEICMPSDHLTDYTPPFIIPTHEGEYEARNSVLVDGGERFGVSAVVFDKHEELIWMGNQGGHVTSYYGPNMQKYTSFQVHDTDEVRDIITVEKCIYVLTKTTLRHQLRRGIPKHTHKSPNMTDMQCLYQVNSTKLLMGGHQDKLIDLDLNKMVETIIPIQEEGCAVLRSGGSSHVACGSANGIVALRDMRAPTKAEHTFRAHTACLSDLDMQGDLLLTCGFTQAVGGVVAEPYIVVWDVRCLRGSNPGQGGWTIHTTSPPLLLHFLPAFSGRAVAASGDGHVSLLHVNAPEERQSIFKVETHSSQCNVMDVASTSQALAFGDQSGHLNLFSPTQNHEPVYNNFSRSTEFADPVEGLPYVRFSDTRFQYSSVPLPQLASGGKWFNELPPEFFRKVYRKPKPVDPEVLKSMKMQGPIGYAPNPKTFKRNQMPYLEESLEEMSISKINGNKVIMQPIPKHYQKLDIRYNKHGPNEELESFNKTGQPGLEATLPNSYCNAMLQVLYYTPPVKATLLAHTCAKEFCLSCELGFLFRMLDTSGGTPCQATNFLRAFRTVPEAAALGLILPDRAPDIRVDLVALIQSWNRFILHQIHYEILETRKKEKELALIANSPPKAPRASNPKERHSLMNGQYSEECQYTELEFLGPTEFDCQEDGELWPGRDEGVDGVGPLQNHESHELNNRQAHREETEISQLFAIGRHQLNRCLKCNKEEGRESVVLACALQYPPTGAGGAGEARGGFVELVRASLAARRSTPAWCDHCSRFTPTTQRGRLLRLPPILAINCGGVTVREKAYWAKGVQKEMSDSKRGGSGKPCRYGLHCARPGCHFKHPDRPNTSQSSSKGSSQESHCMLPLQLLIRLQSDGDVIINDKIETNSAESSPINRPDTSRHKKKYVTTHLEEEYSLSAAVVCVEDNPKNLVAYIHTVRDGETQWFMYNDLCIVPVSTDEVIHFGVWWKTPCVLFYTATGGGQCNGDVSS

Description

Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1.

Catalytic Activity

Exonucleolytic cleavage of poly(A) to 5'-AMP.

Cofactor

a divalent metal cation

Subunit

Forms a heterotrimer with an asymmetric homodimer of the regulatory subunit PAN3 to form the poly(A)-nuclease (PAN) deadenylation complex.

Similarity

Belongs to the peptidase C19 family. PAN2 subfamily.

Keywords

Complete proteome   Cytoplasm   Exonuclease   Hydrolase   Metal-binding   mRNA processing   Nuclease   Nucleus   Reference proteome  

Feature

chain  PAN2-PAN3 deadenylation complex catalytic subunit PAN2

Uniprot

A0A2H1X1K5   A0A2A4J6D2   A0A3S2M593   A0A194Q169   A0A0N0PCF7   A0A0L0CMD5   + More
A0A1A9UZF6   A0A1A9ZQL9   A0A1B0FCG7   A0A0A1WKY4   A0A1A9X9Q1   A0A0A1X745   W8C0U8   A0A034VFC4   W8BE31   A0A034VB09   A0A0K8UJW9   A0A0K8UMD6   A0A026WRW3   B4HSB9   B4JVF5   T1PLY6   A0A0B4LEY4   A0A1I8MWA2   A1Z7K9   A0A1I8MWA8   B4P3E8   A0A0Q5WKR0   B3N839   A0A0J9TWR7   A0A0J9R8L1   B3MIA4   B4KTP8   B4LNP4   A0A0Q9W2X4   A0A3B0JKW5   A0A1I8Q0L5   A0A1I8Q0K1   Q28Y86   A0A2P8XF45   A0A0R3NNZ7   A0A1W4UJY0   A0A195D8A5   A0A1W4UZ14   A0A0L7R7H3   A0A2A3EJH6   A0A0P6J585   B4MIX2   A0A1W4UKF3   A0A088AEH3   A0A1W4UXN6   E2BV67   A0A195BCN5   A0A158NFR0   F4X1C0   A0A151WNW1   A0A1Y1MHS7   B0WEM6   A0A151JSW3   E1ZXV4   A0A182H522   A0A1Q3F7P3   A0A0M4EVE5   A0A0N0BDL8   A0A1B6ER40   A0A0K8VDY8   A0A1B6CJM8   A0A1W4WJ71   A0A1W4WV13   A0A154PQJ1   A0A195DP94   Q7QG93   D6WYL7   A0A182PBX3   A0A182YA82   A0A1B6MGP3   A0A182MD40   A0A182LMD0   A0A1B6M3L7   A0A182K478   A0A182W2E6   A0A182RR09   A0A084WLR5   A0A182T4B7   A0A182UZY4   A0A182HFK7   A0A182IN81   A0A182XG05   A0A0V0G6P6   A0A0P4VMQ2   A0A1J1IDM9   A0A182ULA7   N6U8E4   U4UGF8   A0A023F1S7   A0A224X6Y7  

EC Number

3.1.13.4

Pubmed

26354079    26108605    25830018    24495485    25348373    24508170    + More
30249741    17994087    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    25315136    23932717    24880343    17550304    22936249    15632085    29403074    26999592    20798317    21347285    21719571    28004739    26483478    12364791    18362917    19820115    25244985    20966253    24438588    27129103    23537049    25474469   

EMBL

ODYU01012657    SOQ59086.1    NWSH01002801    PCG67511.1    RSAL01000030    RVE51698.1    + More
KQ459580    KPI99301.1    KQ460571    KPJ13954.1    JRES01000195    KNC33426.1    CCAG010002922    GBXI01015224    JAC99067.1    GBXI01007163    JAD07129.1    GAMC01011296    JAB95259.1    GAKP01018452    JAC40500.1    GAMC01011297    JAB95258.1    GAKP01018451    JAC40501.1    GDHF01025501    GDHF01019213    GDHF01015207    GDHF01008943    JAI26813.1    JAI33101.1    JAI37107.1    JAI43371.1    GDHF01024784    GDHF01009308    JAI27530.1    JAI43006.1    KK107119    QOIP01000009    EZA58790.1    RLU18781.1    CH480816    EDW47014.1    CH916375    EDV98423.1    KA649144    KA649806    AFP64435.1    AE013599    AHN56017.1    BT031142    CM000157    EDW89421.1    CH954177    KQS70830.1    EDV59452.1    CM002911    KMY92380.1    KMY92381.1    CH902619    EDV35949.1    CH933808    EDW09631.1    CH940648    EDW60114.2    KRF79250.1    OUUW01000001    SPP73876.1    CM000071    EAL26079.1    PYGN01002372    PSN30631.1    KRT02814.1    KQ976750    KYN08674.1    KQ414640    KOC66794.1    KZ288230    PBC31624.1    GDUN01000655    JAN95264.1    CH963719    EDW72061.2    GL450824    EFN80359.1    KQ976514    KYM82318.1    ADTU01014572    GL888528    EGI59792.1    KQ982893    KYQ49554.1    GEZM01036261    JAV82847.1    DS231910    EDS45743.1    KQ982011    KYN30679.1    GL435132    EFN73985.1    JXUM01110650    JXUM01110651    KQ565431    KXJ70982.1    GFDL01011469    JAV23576.1    CP012524    ALC41582.1    KQ435855    KOX70612.1    GECZ01029337    JAS40432.1    GDHF01015155    JAI37159.1    GEDC01023612    JAS13686.1    KQ435039    KZC14159.1    KQ980662    KYN14666.1    AAAB01008838    EAA05834.4    KQ971363    EFA09006.2    GEBQ01004860    JAT35117.1    AXCM01001761    GEBQ01009455    JAT30522.1    ATLV01024270    KE525351    KFB51159.1    APCN01006362    GECL01002345    JAP03779.1    GDKW01000752    JAI55843.1    CVRI01000047    CRK98320.1    APGK01038985    KB740966    ENN76921.1    KB632186    ERL89691.1    GBBI01003287    JAC15425.1    GFTR01008209    JAW08217.1   

Proteomes

UP000218220    UP000283053    UP000053268    UP000053240    UP000037069    UP000078200    + More
UP000092445    UP000092444    UP000092443    UP000053097    UP000279307    UP000001292    UP000001070    UP000000803    UP000095301    UP000002282    UP000008711    UP000007801    UP000009192    UP000008792    UP000268350    UP000095300    UP000001819    UP000245037    UP000192221    UP000078542    UP000053825    UP000242457    UP000007798    UP000005203    UP000008237    UP000078540    UP000005205    UP000007755    UP000075809    UP000002320    UP000078541    UP000000311    UP000069940    UP000249989    UP000092553    UP000053105    UP000192223    UP000076502    UP000078492    UP000007062    UP000007266    UP000075885    UP000076408    UP000075883    UP000075882    UP000075881    UP000075920    UP000075900    UP000030765    UP000075901    UP000075903    UP000075840    UP000075880    UP000076407    UP000183832    UP000075902    UP000019118    UP000030742   

PRIDE

A1Z7K9   

Pfam

PF13423   UCH_1        + More
PF00132   Hexapep
PF00929   RNase_T
PF07904   Eaf7

Interpro

IPR038765   Papain-like_cys_pep_sf        + More
IPR036322   WD40_repeat_dom_sf       
IPR028889   USP_dom       
IPR015943   WD40/YVTN_repeat-like_dom_sf       
IPR028881   PAN2_dom       
IPR001451   Hexapep       
IPR011004   Trimer_LpxA-like_sf       
IPR030843   PAN2       
IPR013520   Exonuclease_RNaseT/DNA_pol3       
IPR036397   RNaseH_sf       
IPR012337   RNaseH-like_sf       
IPR012423   Eaf7/MRGBP       
IPR000571   Znf_CCCH       
IPR011047   Quinoprotein_ADH-like_supfam       

SUPFAM

SSF50978   SSF50978        + More
SSF54001   SSF54001       
SSF51161   SSF51161       
SSF53098   SSF53098       
SSF50998   SSF50998       

Gene 3D

2.130.10.10     3.30.420.10    

ProteinModelPortal

A0A2H1X1K5   A0A2A4J6D2   A0A3S2M593   A0A194Q169   A0A0N0PCF7   A0A0L0CMD5   + More
A0A1A9UZF6   A0A1A9ZQL9   A0A1B0FCG7   A0A0A1WKY4   A0A1A9X9Q1   A0A0A1X745   W8C0U8   A0A034VFC4   W8BE31   A0A034VB09   A0A0K8UJW9   A0A0K8UMD6   A0A026WRW3   B4HSB9   B4JVF5   T1PLY6   A0A0B4LEY4   A0A1I8MWA2   A1Z7K9   A0A1I8MWA8   B4P3E8   A0A0Q5WKR0   B3N839   A0A0J9TWR7   A0A0J9R8L1   B3MIA4   B4KTP8   B4LNP4   A0A0Q9W2X4   A0A3B0JKW5   A0A1I8Q0L5   A0A1I8Q0K1   Q28Y86   A0A2P8XF45   A0A0R3NNZ7   A0A1W4UJY0   A0A195D8A5   A0A1W4UZ14   A0A0L7R7H3   A0A2A3EJH6   A0A0P6J585   B4MIX2   A0A1W4UKF3   A0A088AEH3   A0A1W4UXN6   E2BV67   A0A195BCN5   A0A158NFR0   F4X1C0   A0A151WNW1   A0A1Y1MHS7   B0WEM6   A0A151JSW3   E1ZXV4   A0A182H522   A0A1Q3F7P3   A0A0M4EVE5   A0A0N0BDL8   A0A1B6ER40   A0A0K8VDY8   A0A1B6CJM8   A0A1W4WJ71   A0A1W4WV13   A0A154PQJ1   A0A195DP94   Q7QG93   D6WYL7   A0A182PBX3   A0A182YA82   A0A1B6MGP3   A0A182MD40   A0A182LMD0   A0A1B6M3L7   A0A182K478   A0A182W2E6   A0A182RR09   A0A084WLR5   A0A182T4B7   A0A182UZY4   A0A182HFK7   A0A182IN81   A0A182XG05   A0A0V0G6P6   A0A0P4VMQ2   A0A1J1IDM9   A0A182ULA7   N6U8E4   U4UGF8   A0A023F1S7   A0A224X6Y7  

PDB

4D0K     E-value=2.57102e-24,     Score=282

KEGG

101738383    K12571 PAB-dependent poly(A)-specific ribonuclease subunit 2 [EC:3.1.13.4] | (RefSeq) PAB-dependent

PATHWAY

03018    RNA degradation - Bombyx mori (domestic silkworm)

GO

GO:0003676   GO:0004535   GO:0006397   GO:0000289   GO:0031251   GO:0000932   GO:0010606   GO:0006355   GO:0043189   GO:0046872   GO:0005634   GO:0008233   GO:0005515   GO:0015074   GO:0016311   GO:0016791   GO:0006470   GO:0004674   GO:0009058   GO:0016742   GO:0003824  

Subcellular location

Cytoplasm   Shuttles between nucleus and cytoplasm.   With evidence from 1 publications.
P-body   Shuttles between nucleus and cytoplasm.   With evidence from 1 publications.
Nucleus   Shuttles between nucleus and cytoplasm.   With evidence from 1 publications.

Length:

981

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.0522400000000001

Exp number, first 60 AAs:

0

Total prob of N-in:

0.00199

outside

1  -  981

 

 

Pi

229.062206

Theta

176.058956

Tajima's D

0.942018

CLR

0.329966

CSRT

0.640617969101545

Interpretation

Uncertain