Gene

KWMTBOMO04351

Pre Gene Modal

BGIBMGA000832

Annotation

PREDICTED:_fibroblast_growth_factor_receptor_2-like_isoform_X1_[Amyelois_transitella]

Location

Bomo_Chr8(-):4176113-4189232      View in EpiBrowser

Full name

Flap endonuclease 1      

Alternative Name

Flap structure-specific endonuclease 1

Location in the cell

Cytoplasmic   Reliability : 1.977 Nuclear   Reliability : 1.335

CDS

ATGAGTACCAAAAACACAATTATTATAATCAAAGAAGAAGCATCGCAGGAAGAGATGCAAGACTTCTTCCGGGAAATAGAGATGCTGAAGCACGTTGGCTACCACAAACACGTGATTCGTCTCATCGGCTGCTGCACGAGACGAGCCCCGCTCATCGCTCTCCTGGAACACGCTCCAAGAGGTGATCTGCTTTCACTGTTGAGGGCAGCCAGAGGGAGAAGGAAGGAAACTCAGACGTCTGACGTCACTAGGAGAGCAGGCAGCGACATTATCGGAAAACCTTCTGAAGGGGATTCGGATTACACACATTTTAGCGACTCGGATCCGGCACTGTCGGACGAAAAACTTTACATAGACAGCGAAATAAATAAATTAATGAAAAGAGACCATTACGTGGCTGAGCCTGCGTTACATTTAGATAGTACGACTATGAGAGAATACGCGTTGCAAGTCGCTTTAGGAATGAAGCATTTGGAGGGCAGAGGGATCACGCATAGAGATCTGGCGGCTCGGAACATCCTGGTGGATAGCACGGGAGTCTTGAAAGTGGCAGACTTCGGCCTCTCCAGATCAGGAGTGTACGTCCACACGCGTTCCAAGCCAGTGCCGCTGCGATGGCTAGCACCCGAAGCCATTGTCCATTCCCAATATTGCAGCAAAAGCGATGTTTGGGCTTTCGCTGTTTTGCTTTGGGAGATAGCCACCTTGGGTGGTTTCCCTTACGCGGAACTAAGCAACTACCAAGTACCCGCATTTTTGACTGGCGGCGGCAGACTGCCGAAGCCAATGCGAGCGTCAGTGCGCCTCTACGAATTAATGGTCGAATGTTGGTCAGACGATCCACACGACCGGCCAACGTTCGCTCAAATAGTGGACAAACTGGTCATCCAACAGCAGTTGTATGTTGATTTGGAGTGCGTGCTTCCACCGTCAGAAGAAGACATTGGATTCAAAGATTATGATTACACTCTGCCAAGCCCACTCCCCGGGGACACCCAGCTTATGTACAAAGACTGA

Protein

MSTKNTIIIIKEEASQEEMQDFFREIEMLKHVGYHKHVIRLIGCCTRRAPLIALLEHAPRGDLLSLLRAARGRRKETQTSDVTRRAGSDIIGKPSEGDSDYTHFSDSDPALSDEKLYIDSEINKLMKRDHYVAEPALHLDSTTMREYALQVALGMKHLEGRGITHRDLAARNILVDSTGVLKVADFGLSRSGVYVHTRSKPVPLRWLAPEAIVHSQYCSKSDVWAFAVLLWEIATLGGFPYAELSNYQVPAFLTGGGRLPKPMRASVRLYELMVECWSDDPHDRPTFAQIVDKLVIQQQLYVDLECVLPPSEEDIGFKDYDYTLPSPLPGDTQLMYKD

Description

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

Catalytic Activity

ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-[protein]

Cofactor

Mg(2+)

Subunit

Interacts with PCNA. Three molecules of sls bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity.

Similarity

Belongs to the protein kinase superfamily. Tyr protein kinase family.
Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.

Uniprot

A0A2H1WPD8   A0A2A4JIF0   A0A194Q2T8   A0A0N1IAD9   A0A3S2PHM8   A0A212EH28   + More
A0A0L7KY07   A0A182UEF3   A0A1S4H435   A0A1B6DW38   A0A1B6CL53   A0A084VPY9   A0A2J7RRJ4   A0A182FMY7   A0A182Q8T9   Q179Z3   A0A1L8DJG6   A0A1L8DJG5   A0A182JN47   A0A182N8P0   W5J967   Q7Q0U2   V5IAC6   A0A067R3X6   A0A1B6MNC8   A0A182KJM7   A0A1B6LTA0   A0A0L0C7C2   A0A182PVI5   A0A139WC45   B3NAS7   B4Q955   B4I2X7   B4NXU5   A0A1B6IHA6   W8BCC2   A0A3P7E412   W8BST5   Q8IQ00   A0A1I8E8P7   A0A0N4U1K1   A0A0N5DU80   B4NLR3   A0A0J9XQ09   A0A1I7V8S3   A0A3P6TXP8   A0A1S0UJ96   A0A077Z082   A0A0A1XQY7   B3MNY1   A0A0M4ECA8   A0A044UX42   A0A1W4UU21   A0A158RBL0   A0A034VDX9   A0A085NNF4   B4JDX2   B4LTH2   A0A085MGX7   F1KW71   A0A0K8UN28   A0A0A1XCK9   A0A183V2U5   B4G9R0   Q29M57   A0A0R3NY09   A0A1Y1L060   A0A158R5H6   A0A0V1D1Q4   A0A0V1HJV2   A0A0V1HHN3   A0A0V1HIA9   A0A0V1HHS0   A0A0V1HJ59   A0A0V1HHZ4   A0A0V1HIK5   A0A0V1N404   A0A0V1HI03   A0A0V1HJ22   A0A0V1HIJ2   A0A0V0ZYW2   A0A0V0ZYN5   A0A0V0ZYT7  

EC Number

3.1.-.-

Pubmed

26354079    22118469    26227816    12364791    24438588    17510324    + More
20920257    23761445    24845553    20966253    26108605    18362917    19820115    17994087    22936249    17550304    24495485    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    26850696    17885136    25217238    25830018    25348373    24929829    21685128    15632085    28004739   

EMBL

ODYU01010083    SOQ54935.1    NWSH01001362    PCG71546.1    KQ459580    KPI99314.1    + More
KQ460393    KPJ15389.1    RSAL01000030    RVE51708.1    AGBW02014980    OWR40784.1    JTDY01004618    KOB67936.1    AAAB01008980    GEDC01007403    JAS29895.1    GEDC01023203    JAS14095.1    ATLV01015084    KE525002    KFB40033.1    NEVH01000606    PNF43435.1    AXCN02001858    CH477342    EAT43078.1    GFDF01007491    JAV06593.1    GFDF01007492    JAV06592.1    ADMH02002025    ETN59928.1    EAA13998.4    GALX01001420    JAB67046.1    KK852776    KDR16790.1    GEBQ01002509    JAT37468.1    GEBQ01013065    JAT26912.1    JRES01000799    KNC28293.1    KQ971371    KYB25529.1    CH954177    EDV57600.1    CM000361    CM002910    EDX03594.1    KMY87853.1    CH480820    EDW54122.1    CM000157    EDW87516.2    GECU01021405    JAS86301.1    GAMC01010213    JAB96342.1    UYWW01001541    VDM10659.1    GAMC01010214    JAB96341.1    AE014134    AAN10388.3    UYYG01001151    VDN54876.1    CH964274    EDW85302.1    LN856867    CDP93291.1    UYRX01001095    VDK88149.1    JH712198    EJD74947.1    HG805868    CDW53822.1    GBXI01000568    JAD13724.1    CH902620    EDV32168.2    CP012523    ALC39235.1    CMVM020000238    UYYF01004311    VDN02111.1    GAKP01019216    GAKP01019214    JAC39738.1    KL367484    KFD71000.1    CH916368    EDW03492.1    CH940649    EDW63942.2    KL363193    KFD56473.1    JI166901    ADY42125.1    GDHF01024584    JAI27730.1    GBXI01006094    JAD08198.1    UYWY01022611    VDM46386.1    CH479180    EDW29090.1    CH379060    EAL33838.3    KRT04255.1    GEZM01072991    JAV65335.1    JYDI01000055    KRY55434.1    JYDP01000061    KRZ10322.1    KRZ10318.1    KRZ10314.1    KRZ10315.1    KRZ10320.1    KRZ10317.1    KRZ10316.1    JYDO01000010    KRZ78775.1    KRZ10323.1    KRZ10319.1    KRZ10321.1    JYDQ01000055    KRY17832.1    KRY17830.1    KRY17831.1   

Proteomes

UP000218220    UP000053268    UP000053240    UP000283053    UP000007151    UP000037510    + More
UP000075902    UP000030765    UP000235965    UP000069272    UP000075886    UP000008820    UP000075880    UP000075884    UP000000673    UP000007062    UP000027135    UP000075882    UP000037069    UP000075885    UP000007266    UP000008711    UP000000304    UP000001292    UP000002282    UP000270924    UP000000803    UP000093561    UP000038040    UP000274756    UP000046395    UP000007798    UP000006672    UP000095285    UP000277928    UP000030665    UP000007801    UP000092553    UP000024404    UP000192221    UP000046394    UP000276776    UP000001070    UP000008792    UP000050794    UP000267007    UP000008744    UP000001819    UP000046393    UP000054653    UP000055024    UP000054843    UP000054783   

PRIDE

Q8IQ00   

Pfam

PF07714   Pkinase_Tyr        + More
PF12075   KN_motif
PF00752   XPG_N
PF13012   MitMem_reg
PF07679   I-set
PF00041   fn3
PF01398   JAB
PF00867   XPG_I
PF00069   Pkinase
PF06582   Titin_Ig-rpts
PF00435   Spectrin

Interpro

IPR008266   Tyr_kinase_AS        + More
IPR020635   Tyr_kinase_cat_dom       
IPR000719   Prot_kinase_dom       
IPR001245   Ser-Thr/Tyr_kinase_cat_dom       
IPR011009   Kinase-like_dom_sf       
IPR017441   Protein_kinase_ATP_BS       
IPR020683   Ankyrin_rpt-contain_dom       
IPR021939   KN_motif       
IPR036770   Ankyrin_rpt-contain_sf       
IPR002110   Ankyrin_rpt       
IPR013783   Ig-like_fold       
IPR036279   5-3_exonuclease_C_sf       
IPR024969   Rpn11/EIF3F_C       
IPR029060   PIN-like_dom_sf       
IPR006086   XPG-I_dom       
IPR003961   FN3_dom       
IPR007110   Ig-like_dom       
IPR003599   Ig_sub       
IPR013098   Ig_I-set       
IPR019974   XPG_CS       
IPR008271   Ser/Thr_kinase_AS       
IPR036116   FN3_sf       
IPR006084   XPG/Rad2       
IPR008918   HhH2       
IPR000555   JAMM/MPN+_dom       
IPR013106   Ig_V-set       
IPR010939   Titin-rpts       
IPR037518   MPN       
IPR036179   Ig-like_dom_sf       
IPR003598   Ig_sub2       
IPR006085   XPG_DNA_repair_N       
IPR023426   Flap_endonuc       
IPR018159   Spectrin/alpha-actinin       
IPR002017   Spectrin_repeat       

SUPFAM

SSF56112   SSF56112        + More
SSF48403   SSF48403       
SSF88723   SSF88723       
SSF48726   SSF48726       
SSF47807   SSF47807       
SSF49265   SSF49265       

Gene 3D

1.25.40.20     2.60.40.10    

CDD

cd00204  ANK
cd09867  PIN_FEN1
cd00063  FN3

ProteinModelPortal

A0A2H1WPD8   A0A2A4JIF0   A0A194Q2T8   A0A0N1IAD9   A0A3S2PHM8   A0A212EH28   + More
A0A0L7KY07   A0A182UEF3   A0A1S4H435   A0A1B6DW38   A0A1B6CL53   A0A084VPY9   A0A2J7RRJ4   A0A182FMY7   A0A182Q8T9   Q179Z3   A0A1L8DJG6   A0A1L8DJG5   A0A182JN47   A0A182N8P0   W5J967   Q7Q0U2   V5IAC6   A0A067R3X6   A0A1B6MNC8   A0A182KJM7   A0A1B6LTA0   A0A0L0C7C2   A0A182PVI5   A0A139WC45   B3NAS7   B4Q955   B4I2X7   B4NXU5   A0A1B6IHA6   W8BCC2   A0A3P7E412   W8BST5   Q8IQ00   A0A1I8E8P7   A0A0N4U1K1   A0A0N5DU80   B4NLR3   A0A0J9XQ09   A0A1I7V8S3   A0A3P6TXP8   A0A1S0UJ96   A0A077Z082   A0A0A1XQY7   B3MNY1   A0A0M4ECA8   A0A044UX42   A0A1W4UU21   A0A158RBL0   A0A034VDX9   A0A085NNF4   B4JDX2   B4LTH2   A0A085MGX7   F1KW71   A0A0K8UN28   A0A0A1XCK9   A0A183V2U5   B4G9R0   Q29M57   A0A0R3NY09   A0A1Y1L060   A0A158R5H6   A0A0V1D1Q4   A0A0V1HJV2   A0A0V1HHN3   A0A0V1HIA9   A0A0V1HHS0   A0A0V1HJ59   A0A0V1HHZ4   A0A0V1HIK5   A0A0V1N404   A0A0V1HI03   A0A0V1HJ22   A0A0V1HIJ2   A0A0V0ZYW2   A0A0V0ZYN5   A0A0V0ZYT7  

PDB

1FVR     E-value=1.43697e-40,     Score=417

KEGG

101738022    no KO assigned | (RefSeq) fibroblast growth factor receptor-like

GO

GO:0005524   GO:0004713   GO:0016021   GO:0004672   GO:0008284   GO:0004714   GO:0007169   GO:0043235   GO:0005887   GO:0006468   GO:0008409   GO:0017108   GO:0006284   GO:0006260   GO:0005730   GO:0003677   GO:0000287   GO:0005654   GO:0005739   GO:0043137   GO:0006281   GO:0004518   GO:0004674   GO:0009058   GO:0016742   GO:0003824  

Subcellular location

Mitochondrion   Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.   With evidence from 1 publications.
Nucleus   Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.   With evidence from 1 publications.
Nucleolus   Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.   With evidence from 1 publications.
Nucleoplasm   Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.   With evidence from 1 publications.

Length:

338

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.9646

Exp number, first 60 AAs:

0.00436

Total prob of N-in:

0.08924

outside

1  -  338

 

 

Pi

216.538379

Theta

155.856448

Tajima's D

2.522032

CLR

0.358199

CSRT

0.946102694865257

Interpretation

Uncertain