Gene

KWMTBOMO04233  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA004858

Annotation

PREDICTED:_putative_inorganic_phosphate_cotransporter_[Bombyx_mori]

Location

Bomo_Chr8(-):513792-522401      View in EpiBrowser

Full name

Adenylosuccinate synthetase      

Alternative Name

IMP--aspartate ligase

Location in the cell

PlasmaMembrane   Reliability : 4.901

CDS

ATGAGGGCCTGTATGGGAGTTTCTCTGGTCGCCATGACTGATTTTGATGAAGACCCAAATACTAATCTGACAAGCAATTCCAGTAGTAAAGAGTTTGAGGTGCATGGAGTTTTAAATACCTTGATGTTAGTGCCACCGTACCCAATCTTCAGATGGAACAAAAAAATCCAAGATGTCGTGATATCGTCATTCTTATGGGGCTACGTAGTTCTGCAGATACCTGCTGGGCACCTGGCCCACAAATATGGAGCGAAATCCCTGTTAACCGGCGCTATGGCCATAAATTGTATTGCGTCTATTCTATTACCTTTAGGCACTTACTATGGAGGTTGGATCTGCATAGTAATATTCCGTGTTATACAAGGTTTGAGTCAGGCGTGTATAATGCCCGGGATGCACACTTTCTTTGGAAACTGGGCTCCGTTGCATGAACGGGGTCGATTGACTGCTTTGACTTACGGAGGTCAGGCGCTAGGTACCGTATTAGGACTTCCAATGACAGGGTTCATTTCAGCCTCGTCATTAGGCTGGCCTGGAATTTTCAGACTCTACGGGTTGCTATCTGGCTTAATCGGTGCTGTCATCTGGTGGTTATTAGCTGACACTCCAAGTAAGCACACTAGAATATCGGCGATGGAGAAAGAGTATATTGAAAGTGATTTAGGACATAAAAAAGGCCATGACAAGAATAGAATGCCCGTTCCATGGAAGAAAATATTGCGTTCTCAAGGAAGTTATGCTATTATAGTTGCGCATATCGGACACACTTGGGGCCAGCTGATTCTGTACTCCGAGGTACCTGCGTATATGGATAAGGTCATGGGTGTGAACATTAAGGCGAATGGGCTTCTGACAGCCCTTCCATTCATGGTGATGTGGCTTACAAACTTTTTCTTCAGCTGGTTCACGGATATGCTAATTGTAAAGAAGTTTCTGTCCGTCACTAACACCAGAAAAGTCGCTAACTCTATTGGATGTATTCCAGCGGCTATAGGTCTTATATCGCTCGCATATGTTCCGCAAAATATTTATATTATCGAAACAATCCTTGTCTTGGTGTGTGCTGTAAAGGTATCGAGCCATATGGGATTCCAAGTAAGTGAAAGATAG

Protein

MRACMGVSLVAMTDFDEDPNTNLTSNSSSKEFEVHGVLNTLMLVPPYPIFRWNKKIQDVVISSFLWGYVVLQIPAGHLAHKYGAKSLLTGAMAINCIASILLPLGTYYGGWICIVIFRVIQGLSQACIMPGMHTFFGNWAPLHERGRLTALTYGGQALGTVLGLPMTGFISASSLGWPGIFRLYGLLSGLIGAVIWWLLADTPSKHTRISAMEKEYIESDLGHKKGHDKNRMPVPWKKILRSQGSYAIIVAHIGHTWGQLILYSEVPAYMDKVMGVNIKANGLLTALPFMVMWLTNFFFSWFTDMLIVKKFLSVTNTRKVANSIGCIPAAIGLISLAYVPQNIYIIETILVLVCAVKVSSHMGFQVSER

Description

Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP.

Catalytic Activity

GTP + IMP + L-aspartate = GDP + 2 H(+) + N(6)-(1,2-dicarboxyethyl)-AMP + phosphate

Cofactor

Mg(2+)

Subunit

Homodimer.

Similarity

Belongs to the adenylosuccinate synthetase family.

Uniprot

H9J5R8   A0A2A4JKI4   A0A2A4JKL3   A0A2H1VNW8   A0A2H1VP06   A0A2H1VP00   + More
A0A3S2NCJ8   A0A2H1VPY0   A0A212FKV3   A0A194PYB2   A0A194PYB5   A0A0L7KTC4   A0A0L7KS90   A0A2W1BNQ7   A0A194RGR8   A0A194RFB1   A0A2W1BRI7   A0A2A4JIL8   A0A2H1V268   A0A2H1V245   H9JXH9   A0A194RFZ7   H9JXH8   A0A194RFB6   A0A1L8DE98   A0A182K1W8   A0A2J7Q377   A0A2J7Q364   A0A023ESD7   T1PF36   A0A336LZS5   A0A336LY62   A0A182GLJ7   A0A2M4CLX1   A0A084VL23   A0A194Q004   A0A2W1BJ80   Q17LW3   A0A067QS64   A0A1W4WJ07   A0A232FHD3   A0A2M4AF33   K7IRT9   W5JTB8   A0A1Q3FPF7   A0A1Q3FPC2   W8AL50   W8AX47   B0WB17   A0A182MZ46   A0A2A4JK24   A0A2M4AFD6   A0A182MLJ5   Q7PWK4   A0A2H1VD15   A0A182RI61   A0A194Q4I9   A0A182I7D3   A0A182U715   A0A182FN64   A0A1S4GZN6   A0A2A4JRS5   A0A182LMR0   A0A182VKS3   A0A182XNS8   U5EXD9   A0A182W6C0   A0A2M3ZEY8   A0A195C4Y5   A0A2M4BMD9   A0A2M4BMC5   A0A2M4BMA3   A0A182PDV6   A0A212FBF2   A0A2M4BLU2   A0A182Q5D9   A0A0A1XJ44   A0A0A1XHS7   A0A2M4BL92   A0A088AJE0   A0A212FKU6   A0A158NF07   A0A195E864   D6WYK6   A0A2A3E4U8   F4WEN8   A0A195E907   A0A158NF06   V5G377   A0A2H1VWL4   A0A154PQW6   A0A151XIZ3   A0A2H1VIM2   A0A182Y0D9   B4MK15   A0A2W1BL59   F4WEN7   A0A034V4D0   D6WVV3   H9JXI0  

EC Number

6.3.4.4

Pubmed

19121390    22118469    26354079    26227816    28756777    24945155    + More
25315136    26483478    24438588    17510324    24845553    28648823    20075255    20920257    23761445    24495485    12364791    20966253    25830018    21347285    18362917    19820115    21719571    25244985    17994087    25348373   

EMBL

BABH01025059    BABH01025060    NWSH01001263    PCG71943.1    PCG71942.1    ODYU01003583    + More
SOQ42529.1    ODYU01003582    SOQ42527.1    SOQ42528.1    RSAL01000225    RVE43970.1    ODYU01003747    SOQ42903.1    AGBW02007960    OWR54367.1    KQ459585    KPI98326.1    KPI98327.1    JTDY01006116    KOB66274.1    JTDY01006556    KOB65894.1    KZ150053    PZC74370.1    KQ460211    KPJ16520.1    KPJ16518.1    PZC74373.1    PCG71945.1    ODYU01000335    SOQ34909.1    ODYU01000336    SOQ34910.1    BABH01032693    KPJ16522.1    KPJ16523.1    GFDF01009316    JAV04768.1    NEVH01019069    PNF23027.1    PNF23028.1    GAPW01001428    JAC12170.1    KA647304    AFP61933.1    UFQS01000360    UFQT01000360    SSX03128.1    SSX23494.1    UFQS01000292    UFQT01000292    SSX02540.1    SSX22914.1    JXUM01072235    JXUM01072236    KQ562709    KXJ75293.1    GGFL01002003    MBW66181.1    ATLV01014397    KE524970    KFB38667.1    KPI98324.1    PZC74361.1    CH477210    EAT47708.1    KK853091    KDR11545.1    NNAY01000187    OXU30176.1    GGFK01005927    MBW39248.1    ADMH02000203    ETN67381.1    GFDL01005653    JAV29392.1    GFDL01005671    JAV29374.1    GAMC01017148    GAMC01017145    JAB89410.1    GAMC01017147    GAMC01017146    JAB89408.1    DS231876    EDS41975.1    PCG71944.1    GGFK01006136    MBW39457.1    AXCM01007649    AAAB01008984    EAA14814.5    ODYU01001711    SOQ38292.1    KPI98325.1    APCN01003380    NWSH01000802    PCG74112.1    GANO01002583    JAB57288.1    GGFM01006318    MBW27069.1    KQ978287    KYM95665.1    GGFJ01004982    MBW54123.1    GGFJ01005003    MBW54144.1    GGFJ01004983    MBW54124.1    AGBW02009333    OWR51071.1    GGFJ01004823    MBW53964.1    AXCN02000794    GBXI01003739    JAD10553.1    GBXI01003780    JAD10512.1    GGFJ01004684    MBW53825.1    OWR54368.1    ADTU01013694    ADTU01013695    KQ979479    KYN21311.1    KQ971362    EFA07863.1    KZ288409    PBC26101.1    GL888103    EGI67401.1    KYN21312.1    ADTU01013693    GALX01004011    JAB64455.1    ODYU01004887    SOQ45220.1    KQ435056    KZC14291.1    KQ982080    KYQ60190.1    ODYU01002771    SOQ40673.1    CH963846    EDW72454.1    PZC74374.1    EGI67400.1    GAKP01020827    GAKP01020826    JAC38126.1    KQ971358    EFA08237.1    BABH01032689    BABH01032690   

Proteomes

UP000005204    UP000218220    UP000283053    UP000007151    UP000053268    UP000037510    + More
UP000053240    UP000075881    UP000235965    UP000095301    UP000069940    UP000249989    UP000030765    UP000008820    UP000027135    UP000192223    UP000215335    UP000002358    UP000000673    UP000002320    UP000075884    UP000075883    UP000007062    UP000075900    UP000075840    UP000075902    UP000069272    UP000075882    UP000075903    UP000076407    UP000075920    UP000078542    UP000075885    UP000075886    UP000005203    UP000005205    UP000078492    UP000007266    UP000242457    UP000007755    UP000076502    UP000075809    UP000076408    UP000007798   

PRIDE

H9J5R8    H9JXH9    H9JXH8    H9JXI0   

Pfam

PF07690   MFS_1        + More
PF00709   Adenylsucc_synt

Interpro

IPR001114   Adenylosuccinate_synthetase        + More
IPR042110   Adenylosuccinate_synth_dom2       
IPR042109   Adenylosuccinate_synth_dom1       
IPR027417   P-loop_NTPase       
IPR020846   MFS_dom       
IPR036259   MFS_trans_sf       
IPR033128   Adenylosuccin_syn_Lys_AS       
IPR011701   MFS       
IPR027378   Nucleotide_channel_N       
IPR005829   Sugar_transporter_CS       

SUPFAM

SSF103473   SSF103473        + More
SSF52540   SSF52540       

Gene 3D

1.10.300.10     3.40.440.10     1.20.120.540    

CDD

cd06174  MFS

ProteinModelPortal

H9J5R8   A0A2A4JKI4   A0A2A4JKL3   A0A2H1VNW8   A0A2H1VP06   A0A2H1VP00   + More
A0A3S2NCJ8   A0A2H1VPY0   A0A212FKV3   A0A194PYB2   A0A194PYB5   A0A0L7KTC4   A0A0L7KS90   A0A2W1BNQ7   A0A194RGR8   A0A194RFB1   A0A2W1BRI7   A0A2A4JIL8   A0A2H1V268   A0A2H1V245   H9JXH9   A0A194RFZ7   H9JXH8   A0A194RFB6   A0A1L8DE98   A0A182K1W8   A0A2J7Q377   A0A2J7Q364   A0A023ESD7   T1PF36   A0A336LZS5   A0A336LY62   A0A182GLJ7   A0A2M4CLX1   A0A084VL23   A0A194Q004   A0A2W1BJ80   Q17LW3   A0A067QS64   A0A1W4WJ07   A0A232FHD3   A0A2M4AF33   K7IRT9   W5JTB8   A0A1Q3FPF7   A0A1Q3FPC2   W8AL50   W8AX47   B0WB17   A0A182MZ46   A0A2A4JK24   A0A2M4AFD6   A0A182MLJ5   Q7PWK4   A0A2H1VD15   A0A182RI61   A0A194Q4I9   A0A182I7D3   A0A182U715   A0A182FN64   A0A1S4GZN6   A0A2A4JRS5   A0A182LMR0   A0A182VKS3   A0A182XNS8   U5EXD9   A0A182W6C0   A0A2M3ZEY8   A0A195C4Y5   A0A2M4BMD9   A0A2M4BMC5   A0A2M4BMA3   A0A182PDV6   A0A212FBF2   A0A2M4BLU2   A0A182Q5D9   A0A0A1XJ44   A0A0A1XHS7   A0A2M4BL92   A0A088AJE0   A0A212FKU6   A0A158NF07   A0A195E864   D6WYK6   A0A2A3E4U8   F4WEN8   A0A195E907   A0A158NF06   V5G377   A0A2H1VWL4   A0A154PQW6   A0A151XIZ3   A0A2H1VIM2   A0A182Y0D9   B4MK15   A0A2W1BL59   F4WEN7   A0A034V4D0   D6WVV3   H9JXI0  

PDB

6E9O     E-value=4.77197e-13,     Score=180

KEGG

101739720    K12301 MFS transporter, ACS family, solute carrier family 17 (sodium-dependent inorganic phosphate c

PATHWAY

04142    Lysosome - Bombyx mori (domestic silkworm)

GO

GO:0044208   GO:0005525   GO:0004019   GO:0016021   GO:0055085   GO:0000287   GO:0005737   GO:0005215   GO:0005886   GO:0001666   GO:0048190   GO:0007186   GO:0006384   GO:0042555   GO:0006260   GO:0004672   GO:0048384  

Subcellular location

Cytoplasm  

Length:

369

Number of predicted TMHs:

8

Exp number of AAs in TMHs:

161.42875

Exp number, first 60 AAs:

2.05679

Total prob of N-in:

0.96534

inside

1  -  58

TMhelix

59  -  76

outside

77  -  90

TMhelix

91  -  113

inside

114  -  147

TMhelix

148  -  170

outside

171  -  179

TMhelix

180  -  199

inside

200  -  243

TMhelix

244  -  263

outside

264  -  277

TMhelix

278  -  300

inside

301  -  319

TMhelix

320  -  339

outside

340  -  342

TMhelix

343  -  360

inside

361  -  369

 

 

Pi

207.437293

Theta

152.724049

Tajima's D

0.637372

CLR

0.406455

CSRT

0.551372431378431

Interpretation

Uncertain