Gene

KWMTBOMO04175

Pre Gene Modal

BGIBMGA008726

Annotation

PREDICTED:_kinesin-like_protein_KLP2_[Bombyx_mori]

Location

Bomo_Chr7(+):12891332-12900916      View in EpiBrowser

Full name

Kinesin-like protein       + More
Kinesin-like protein Klp61F      

Alternative Name

Bipolar kinesin KRP-130

Location in the cell

Nuclear   Reliability : 2.508

CDS

ATGACGTCGGAGAAAGGATGTCGGAAAGAAAAGAATCAAAATATCCAAGTGTTTGTTCGTTTGAGACCGCTCAACCAACGGGAACGAGACCTCAAATCTTTGGGGGTAGTTGAAGTGCACAATAACAAGGAGGTTGTTGTGAGGATCTCACAGCAGAACAGCATTACCAAGAAGTTCACCTTTGACAGAGCATTCGCTCCTTATGCTAACCAGGTGGAAGTTTACCAAGAGGTGGTCAGCCCACTCATTGAAGAAGTGCTGGCTGGCTACAATTGCACTGTCTTTGCTTACGGTCAGACAGGCACTGGCAAGACCCACACAATGGTCGGAGAAAATACTGGTGATGAAACTACTTGGCAAAAGGATCCATTAGCCGGTATAATACCACGTGCGCTGAGCCAACTTTTTGATGAGCTTCGTATTTCTAACACCGAGTACACGGTCCGTGTGTCTTACTTGGAACTATATAATGAAGAGCTCTTTGACTTGCTGGCAACATCTGAAGACAATTCCAAGCTCAGGATTTATGAAGATGTCACTAGGAAAGGATCTAATATTGTAAATGGGCTAGAAGAGATAACTGTCTACAACAAAAAGGAAGTGTTCAGAATTATGGCCCAAGGACAGGAAAGGAAGAAAGTGGCTTCGACACTCATGAACGCACAGTCCAGTCGCTCCCACACCGTCTTCACGATCGTAGTCCACATGAAAGAAAACAGTCCTGAGGGCGAGGAGCTGGTGAAGATAGGCAAGCTAAACCTGGTGGATCTCGCTGGCTCAGAGAACATCAGCAAAGCTGGTTCTGACAACCCAGCCAAGCGGGAGAGGGCCCGGGAGTGTGTGAACATCAATCAGTCTCTGCTAACTCTTGGGAGGGTCATCACTGCACTTGTGGAAAGGCATCCTCATGTTCCTTATAGAGAATCAAAACTAACCAGGATCCTGCAAGAATCCCTCGGTGGTAGAACCAAAACTTCAATTATTGCTACAATCTCACCTGGACATAAGGACCTGGAAGAGACCATGAGCACTCTTGAGTATGCTCACAGAGCAAAGAACATTCAGAACAAGCCTGAGATCAACCAGAAAATGACAAAGAAAGCTATATTGAAAGAATATGCTGAAGAAATTGACAGATTGAAGAGGGATCTGCAAGCTGCAAGGGATAAAAATGGAGTATATCTAGCGAATGAAACCTTCGCCGAGATAACATTGAAACAGGAAGAACAAAGAAAAGAAATACAAGAACTCTTACTAAAGAAGAGAGCGATGGAAGAGGAAAGAGATAAGATGGAGAATGTCTTTAGTAACTTGAGTCGACAGTTGGAGTGCAGGGATGCCGAGCTCAATGAAACAAAGAATAAATTGAACAAAACAAATGCGAGATTAGCAGACACCTGTAATGTTCTGAAGACAACAAAACAACAATTTGAAGAGCAACAGTATTTAGTTCTGAAACATCGTGATACAGAACAAACTCTCACTCAACAAGCGAATCAGTTGCTGGCCACAGTAGACGTCGCCACCACACATGTCGCTTGCTTGCATGATAGTGTTGAAAAGAGGAGGCAATTAGAAAATGTTAATTTAGAAGTGACTAAAACTTACGTTGAACAATCCTCACAACACCGGGAGCACCTCACCGCTGGCATTGGGCATTACGTAGAGAACATCACTGCGGCACTGAAATCCCTCGATGATGCTATGAATGACTATACAGACAATAACACTCGCACACAAGAAGAAAGCAAACTTAAATTGGAAGAGTTAATTGGAATGATGGAGACAACTATCAATGAGATAAGATCCCTAGCATTGGACACACAGACCTCATTGGTTACAACAACAGAACAACATCGAAACGAAGCTCGGTCGATGGTAAGCGACCACAAGATGGCAATCTGTGCTGTTGGAGATTCATTAGTGGAGAGTGCTAACGATACCTTAGTCGCGGCCACCAACGTGGACCTCAGAGGACACATCGCTAGATTTGTCGAGCAGTGGTACAGGGGAGCGGCGGAGCGCGTGACGTCACTGCAGCGTGCGCACGCAGCGTTCGACACGCGCGGGCGGGACGCGCGGCTGGCACGCGGCGTGCTGCACGCGGCCCGGCGGGACGGCGCGCGGGAGCAGGCGGCGCGCGCGCGTCTCGCGGTGCAAGCGTGTCTACAGGTAGTCAATCAAATCAAATGA

Protein

MTSEKGCRKEKNQNIQVFVRLRPLNQRERDLKSLGVVEVHNNKEVVVRISQQNSITKKFTFDRAFAPYANQVEVYQEVVSPLIEEVLAGYNCTVFAYGQTGTGKTHTMVGENTGDETTWQKDPLAGIIPRALSQLFDELRISNTEYTVRVSYLELYNEELFDLLATSEDNSKLRIYEDVTRKGSNIVNGLEEITVYNKKEVFRIMAQGQERKKVASTLMNAQSSRSHTVFTIVVHMKENSPEGEELVKIGKLNLVDLAGSENISKAGSDNPAKRERARECVNINQSLLTLGRVITALVERHPHVPYRESKLTRILQESLGGRTKTSIIATISPGHKDLEETMSTLEYAHRAKNIQNKPEINQKMTKKAILKEYAEEIDRLKRDLQAARDKNGVYLANETFAEITLKQEEQRKEIQELLLKKRAMEEERDKMENVFSNLSRQLECRDAELNETKNKLNKTNARLADTCNVLKTTKQQFEEQQYLVLKHRDTEQTLTQQANQLLATVDVATTHVACLHDSVEKRRQLENVNLEVTKTYVEQSSQHREHLTAGIGHYVENITAALKSLDDAMNDYTDNNTRTQEESKLKLEELIGMMETTINEIRSLALDTQTSLVTTTEQHRNEARSMVSDHKMAICAVGDSLVESANDTLVAATNVDLRGHIARFVEQWYRGAAERVTSLQRAHAAFDTRGRDARLARGVLHAARRDGAREQAARARLAVQACLQVVNQIK

Description

Important role in mitotic dividing cells (PubMed:8227131). Microtubule motor required for spindle body separation (PubMed:8918872). Slow plus-end directed microtubule motor capable of cross-linking and sliding apart antiparallel microtubules, thereby pushing apart the associated spindle poles during spindle assembly and function (PubMed:8918872, PubMed:8589456, PubMed:19062285). Forms cross-links between microtubules within interpolar microtubule bundles (PubMed:9885249, PubMed:19158379). Contributes to the length of the metaphase spindle, maintains the prometaphase spindle by antagonizing Ncd, drives anaphase B, and also contributes to normal chromosome congression, kinetochore spacing, and anaphase A rates (PubMed:19158379). Displays microtubule-stimulated ATPase activity (PubMed:8589456). Required for normal fusome organization (PubMed:10469596). Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface (PubMed:23857769).

Subunit

Homotetramer (PubMed:8538794, PubMed:9885249, PubMed:24714498). Consists of two pairs of polypeptides associated by coiled-coil interactions to form two homodimers (PubMed:8538794). The homodimers are linked by lateral interactions between their coiled-coil regions to form a bipolar homotetramer consisting of a central rod with two motor domains projecting from either end (PubMed:8538794). Parallel coiled coils extend from each pair of motor heads, switch to two antiparallel coiled coils in the central region and then back to parallel coiled coils (PubMed:24714498). Interacts with Wee1 (PubMed:19800237).

Similarity

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. BimC subfamily.

Keywords

3D-structure   ATP-binding   Cell cycle   Cell division   Coiled coil   Complete proteome   Cytoplasm   Cytoskeleton   Direct protein sequencing   Microtubule   Mitosis   Motor protein   Nucleotide-binding   Phosphoprotein   Reference proteome  

Feature

chain  Kinesin-like protein Klp61F

Uniprot

H9JGS9   A0A2A4K3W6   A0A3S2NYV7   A0A2H1WJI6   A0A212EIG2   A0A194PW97   + More
A0A194RCC7   A0A2W1BXA5   A0A1I8P378   P46863   Q0PQ30   B4HVQ1   A0A0J9RLX7   B4QLY4   B3NB76   Q0PQ31   B3M537   B4LG86   B4PD55   B4J2F6   A0A3B0JRK3   Q29EV0   A0A1W4W768   B4L0M5   A0A0L0CR22   A0A1B0A2F7   A0A1I8NCE9   A0A1A9W4K9   A0A0A1WPI6   W8BFY4   A0A0M4EJL6   A0A0K8TXE6   A0A336LMR6   A0A2J7QSN9   A0A1B0BAB5   A0A182KRE0   A0A182TUA3   A0A182IP87   A0A182I3C6   A0A182V4Z5   Q7QJG2   A0A1B0C8R8   A0A182X3P3   A0A182QBE6   A0A182XYY0   A0A182FF61   A0A232FJ27   K7J300   A0A182PQD4   A0A182KAR0   B4MXY8   A0A182MQK1   A0A182RSQ1   A0A084WNC6   A0A195F4N0   A0A158NE35   A0A182WJ67   A0A0P5C224   A0A0P5C278   A0A0N8A9Q9   A0A0P5EK76   A0A0P6GJC8   A0A0P6FH74   A0A0P5H1R0   A0A0P5WQL3   A0A0P4YVL0   A0A0P5PB87   A0A0P5GPT4   A0A0P5AHG7   A0A182NHT7   A0A195BP88   A0A0P5GVE6   A0A0P4WWK6   A0A0N8BKA1   A0A0P5F0I5   A0A0P4Z4S7   A0A0P4Z622   A0A0P5IL51   A0A0P6IHI9   A0A0N8CZQ2   A0A0N8CLC8   A0A0P5TY16   A0A0P5L4P1   A0A0P5EV44   A0A0P6CX10   A0A0N8EBY4   F4WZ40   A0A0P5WKJ5   A0A0P5R5R9   E9IYU5   A0A0P5XN86   E9GVD2   A0A182GX94   A0A0P4YC33   A0A0P5WWF5   Q16HA8  

Pubmed

19121390    22118469    26354079    28756777    8227131    10731132    + More
12537572    12537569    1924306    8918872    8589456    8538794    10469596    9885249    17372656    19062285    18327897    19800237    19158379    23857769    19285086    24714498    17994087    22936249    17550304    15632085    18057021    26108605    25315136    25830018    24495485    20966253    12364791    25244985    28648823    20075255    24438588    21347285    21719571    21282665    21292972    26483478    17510324   

EMBL

BABH01034392    BABH01034393    BABH01034394    BABH01034395    BABH01034396    NWSH01000158    + More
PCG78945.1    RSAL01000020    RVE52567.1    ODYU01009057    SOQ53198.1    AGBW02014657    OWR41268.1    KQ459589    KPI97651.1    KQ460367    KPJ15498.1    KZ149929    PZC77440.1    U01842    AE014296    AY069442    M74428    DQ782382    ABG91087.1    CH480817    EDW50016.1    CM002912    KMY96772.1    CM000363    EDX08770.1    CH954178    EDV50129.1    DQ782381    ABG91086.1    CH902618    EDV39516.1    CH940647    EDW69394.1    CM000159    EDW92803.1    CH916366    EDV96015.1    OUUW01000009    SPP84767.1    CH379070    EAL29960.1    CH933809    EDW18102.1    KRG05979.1    JRES01000036    KNC34696.1    GBXI01013535    JAD00757.1    GAMC01010697    GAMC01010693    JAB95858.1    CP012525    ALC44238.1    GDHF01033356    JAI18958.1    UFQT01000063    SSX19266.1    NEVH01011876    PNF31587.1    JXJN01010891    APCN01000205    AAAB01008807    EAA04655.4    AJWK01001311    AXCN02000377    NNAY01000174    OXU30307.1    CH963876    EDW76977.1    AXCM01000691    ATLV01024587    KE525352    KFB51720.1    KQ981820    KYN35132.1    ADTU01012730    GDIP01177228    JAJ46174.1    GDIP01177227    JAJ46175.1    GDIP01168665    JAJ54737.1    GDIP01145275    JAJ78127.1    GDIQ01033903    JAN60834.1    GDIQ01047845    JAN46892.1    GDIQ01233229    JAK18496.1    GDIP01083030    JAM20685.1    GDIP01232965    JAI90436.1    GDIQ01131026    JAL20700.1    GDIQ01244876    GDIQ01240941    GDIQ01220452    GDIQ01216987    GDIQ01140809    GDIQ01050761    JAK06849.1    GDIP01219520    GDIP01199467    GDIQ01187494    JAJ23935.1    JAK64231.1    KQ976424    KYM88325.1    GDIQ01242380    JAK09345.1    GDIP01254011    JAI69390.1    GDIQ01169451    JAK82274.1    GDIQ01267544    JAJ84180.1    GDIP01217964    GDIQ01032596    JAJ05438.1    JAN62141.1    GDIP01221773    JAJ01629.1    GDIQ01211844    JAK39881.1    GDIQ01010957    JAN83780.1    GDIP01083031    JAM20684.1    GDIP01120423    JAL83291.1    GDIP01120377    JAL83337.1    GDIQ01180449    JAK71276.1    GDIQ01267543    JAJ84181.1    GDIQ01086480    JAN08257.1    GDIQ01041796    JAN52941.1    GL888465    EGI60543.1    GDIP01084777    JAM18938.1    GDIQ01110622    JAL41104.1    GL767051    EFZ14248.1    GDIP01069628    JAM34087.1    GL732568    EFX76397.1    JXUM01094920    JXUM01094921    JXUM01094922    JXUM01094923    KQ564161    KXJ72656.1    GDIP01230567    JAI92834.1    GDIP01080981    JAM22734.1    CH478187    EAT33633.1   

Proteomes

UP000005204    UP000218220    UP000283053    UP000007151    UP000053268    UP000053240    + More
UP000095300    UP000000803    UP000001292    UP000000304    UP000008711    UP000007801    UP000008792    UP000002282    UP000001070    UP000268350    UP000001819    UP000192221    UP000009192    UP000037069    UP000092445    UP000095301    UP000091820    UP000092553    UP000235965    UP000092460    UP000075882    UP000075902    UP000075880    UP000075840    UP000075903    UP000007062    UP000092461    UP000076407    UP000075886    UP000076408    UP000069272    UP000215335    UP000002358    UP000075885    UP000075881    UP000007798    UP000075883    UP000075900    UP000030765    UP000078541    UP000005205    UP000075920    UP000075884    UP000078540    UP000007755    UP000000305    UP000069940    UP000249989    UP000008820   

PRIDE

H9JGS9    P46863    Q0PQ30    Q0PQ31   

Pfam

PF00225   Kinesin        + More
PF13931   Microtub_bind
PF13873   Myb_DNA-bind_5

Interpro

IPR019821   Kinesin_motor_CS        + More
IPR027417   P-loop_NTPase       
IPR036961   Kinesin_motor_dom_sf       
IPR001752   Kinesin_motor_dom       
IPR027640   Kinesin-like_fam       
IPR025901   Kinesin-assoc_MT-bd_dom       
IPR028002   Myb_DNA-bind_5       

SUPFAM

SSF52540   SSF52540       

Gene 3D

3.40.850.10    

ProteinModelPortal

H9JGS9   A0A2A4K3W6   A0A3S2NYV7   A0A2H1WJI6   A0A212EIG2   A0A194PW97   + More
A0A194RCC7   A0A2W1BXA5   A0A1I8P378   P46863   Q0PQ30   B4HVQ1   A0A0J9RLX7   B4QLY4   B3NB76   Q0PQ31   B3M537   B4LG86   B4PD55   B4J2F6   A0A3B0JRK3   Q29EV0   A0A1W4W768   B4L0M5   A0A0L0CR22   A0A1B0A2F7   A0A1I8NCE9   A0A1A9W4K9   A0A0A1WPI6   W8BFY4   A0A0M4EJL6   A0A0K8TXE6   A0A336LMR6   A0A2J7QSN9   A0A1B0BAB5   A0A182KRE0   A0A182TUA3   A0A182IP87   A0A182I3C6   A0A182V4Z5   Q7QJG2   A0A1B0C8R8   A0A182X3P3   A0A182QBE6   A0A182XYY0   A0A182FF61   A0A232FJ27   K7J300   A0A182PQD4   A0A182KAR0   B4MXY8   A0A182MQK1   A0A182RSQ1   A0A084WNC6   A0A195F4N0   A0A158NE35   A0A182WJ67   A0A0P5C224   A0A0P5C278   A0A0N8A9Q9   A0A0P5EK76   A0A0P6GJC8   A0A0P6FH74   A0A0P5H1R0   A0A0P5WQL3   A0A0P4YVL0   A0A0P5PB87   A0A0P5GPT4   A0A0P5AHG7   A0A182NHT7   A0A195BP88   A0A0P5GVE6   A0A0P4WWK6   A0A0N8BKA1   A0A0P5F0I5   A0A0P4Z4S7   A0A0P4Z622   A0A0P5IL51   A0A0P6IHI9   A0A0N8CZQ2   A0A0N8CLC8   A0A0P5TY16   A0A0P5L4P1   A0A0P5EV44   A0A0P6CX10   A0A0N8EBY4   F4WZ40   A0A0P5WKJ5   A0A0P5R5R9   E9IYU5   A0A0P5XN86   E9GVD2   A0A182GX94   A0A0P4YC33   A0A0P5WWF5   Q16HA8  

PDB

2WBE     E-value=1.22695e-129,     Score=1189

KEGG

692748    K10398 kinesin family member 11 | (RefSeq) Kinesin-5, Klp2, Klp61f; kinesin like protein 2

GO

GO:0007018   GO:0008017   GO:0003777   GO:0005524   GO:0003774   GO:0005874   GO:1990498   GO:0005875   GO:0005783   GO:0005818   GO:0051301   GO:0097431   GO:0008574   GO:0000278   GO:0045478   GO:0072686   GO:0051289   GO:0007052   GO:0051299   GO:0005794   GO:0045169   GO:0005634   GO:0005737   GO:0007100   GO:0001578   GO:0042998   GO:0005819   GO:0009306   GO:0005871   GO:0031535   GO:0007030   GO:0090307   GO:0016887   GO:0005876   GO:0016021   GO:0042555   GO:0006260   GO:0004672   GO:0048384  

PANTHER

PTHR24115  

Subcellular location

Cytoplasm   In somatic cells, cytoplasmic during interphase, localized to centrosomal asters at the onset of mitosis in prophase and associated with spindle structures during the remainder of mitosis (PubMed:10469596). In male and female germ cells, associates with fusomes during interphase, then localizes to centrosomal asters during prophase and to spindles in metaphase (PubMed:10469596). Coincident with spindle microtubules from prophase to metaphase and, as mitosis proceeds, found both at the spindle poles and along the spindle fibers (PubMed:8589456).   With evidence from 5 publications.
Cytoskeleton   In somatic cells, cytoplasmic during interphase, localized to centrosomal asters at the onset of mitosis in prophase and associated with spindle structures during the remainder of mitosis (PubMed:10469596). In male and female germ cells, associates with fusomes during interphase, then localizes to centrosomal asters during prophase and to spindles in metaphase (PubMed:10469596). Coincident with spindle microtubules from prophase to metaphase and, as mitosis proceeds, found both at the spindle poles and along the spindle fibers (PubMed:8589456).   With evidence from 5 publications.
Spindle   In somatic cells, cytoplasmic during interphase, localized to centrosomal asters at the onset of mitosis in prophase and associated with spindle structures during the remainder of mitosis (PubMed:10469596). In male and female germ cells, associates with fusomes during interphase, then localizes to centrosomal asters during prophase and to spindles in metaphase (PubMed:10469596). Coincident with spindle microtubules from prophase to metaphase and, as mitosis proceeds, found both at the spindle poles and along the spindle fibers (PubMed:8589456).   With evidence from 5 publications.
Spindle pole   In somatic cells, cytoplasmic during interphase, localized to centrosomal asters at the onset of mitosis in prophase and associated with spindle structures during the remainder of mitosis (PubMed:10469596). In male and female germ cells, associates with fusomes during interphase, then localizes to centrosomal asters during prophase and to spindles in metaphase (PubMed:10469596). Coincident with spindle microtubules from prophase to metaphase and, as mitosis proceeds, found both at the spindle poles and along the spindle fibers (PubMed:8589456).   With evidence from 5 publications.

Length:

730

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.01034

Exp number, first 60 AAs:

0

Total prob of N-in:

0.00111

outside

1  -  730

 

 

Pi

26.792513

Theta

25.667299

Tajima's D

1.300669

CLR

0.360958

CSRT

0.744262786860657

Interpretation

Uncertain