Gene

KWMTBOMO04172  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA008668

Annotation

clip_domain_serine_protease_4_precursor_[Bombyx_mori]

Location

Bomo_Chr7(-):12788321-12792482      View in EpiBrowser

Full name

Venom serine protease Bi-VSP       + More
Venom protease      

Alternative Name

Thrombin-like enzyme

Location in the cell

Cytoplasmic   Reliability : 1.804 Extracellular   Reliability : 1.502

CDS

ATGTTGAATACGTATTTCACTCAAAATAGTACTCGTATTTGCCACGGCTACACCAAATTTCACGAAGACTTCCGAAGAAATTATCGCGAATCCTTAATTTCCAGGTACTTAGTGCGAGTGGGAGAGCTGGATCTTGCCAGAGACGATGAAGGTGCTACTCCTGTGGACGTTCTCATTAAGACGAAGATCAAGCACGAGCAATACGATGCCGCCTCTTATACCAACGACATCGGCATCCTGGTGCTGGAGAAGGACGTACCTATTACAGACTTGATTAAGCCTATCTGCATCCCCAAGGACACAGAGCTTCGCTCCCGCAGCTTCGAGGACTACAACCCCATCATAGCCGGCTGGGGGGACACAGAGTTCCGCGGTCCATCCGCAACGCACCTCCAAGTCCTGCAGCTGCCTGTGGTCAGCAACGACTTTTGTGCTCAAGCGTACTCGCCATATAAGAATCAGAAGATCGATGAGAGGGTGCTCTGCGCTGGGTACAAGAAGGGGGGCAAGGACGCCTGTCAGGGGGACAGCGGGGGCCCTCTGATGCAGCCCATTTGGAACTCGCAGACCTACAAGACCTACTTCTACCAGATCGGAGTGGTCTCCTTCGGCAAGAAATGCGCTGAGGCTGGATTCCCCGGCGTCTATGCCAGGGTCACGCACTTCGTTCCCTGGATCCAAGAGAAGGTTGTGGGTCATGCGTGA

Protein

MLNTYFTQNSTRICHGYTKFHEDFRRNYRESLISRYLVRVGELDLARDDEGATPVDVLIKTKIKHEQYDAASYTNDIGILVLEKDVPITDLIKPICIPKDTELRSRSFEDYNPIIAGWGDTEFRGPSATHLQVLQLPVVSNDFCAQAYSPYKNQKIDERVLCAGYKKGGKDACQGDSGGPLMQPIWNSQTYKTYFYQIGVVSFGKKCAEAGFPGVYARVTHFVPWIQEKVVGHA

Description

Multifunctional venom serine protease. In insects, it acts as an arthropod prophenoloxidase-activating factor, thereby triggering the phenoloxidase cascade. When injected into larvae, it induces a lethal melanization response in target insects by modulating the innate immune response. In mammals, it converts fibrinogen into fibrin, activates prothrombin, and also degrades fibrin. In mammal, it may act in a cooperative manner with the serine protease inhibitor Bi-KTI (AC G3LH89) to promote the spread of bee venom under anti-bleeding conditions.

Miscellaneous

Does not activate plasminogen.

Similarity

Belongs to the peptidase S1 family.
Belongs to the peptidase S1 family. CLIP subfamily.

Keywords

Blood coagulation cascade activating toxin   Calcium   Direct protein sequencing   Disulfide bond   Fibrinolytic toxin   Glycoprotein   Hemostasis impairing toxin   Hydrolase   Metal-binding   Protease   Prothrombin activator   Secreted   Serine protease   Signal   Toxin   Zymogen   Allergen  

Feature

chain  Venom serine protease Bi-VSP

Uniprot

Q8MP08   H9JGM1   A0A2A4JS27   A0A2W1BQQ9   Q8I927   Q5MPB7   + More
Q5MPB8   Q8I925   Q8I926   A0A212F528   A0A2H1WWB5   A0A194PXA8   A0A212EIG1   A0A212EPS0   A0A212EIF7   A0A2A3ENM0   A0A1I9UVU8   A0A212EPV1   A0A154PEP1   A0A088ADM5   A0A2J7R078   A0A2J7R073   A0A0M8ZP71   A0A1V1FYF4   A0A1W4WNW1   A0A1W4WNW0   B0WB63   A0A1Y1M8P7   A0A067RFG3   K7J1M7   E2BNP2   A0A182GQG7   Q17J63   A0A0L7RGN5   E9J1L2   A0A1S4F0R9   A0A232EUU8   A0A0P4VP51   A0A1B6EF69   T1HGB7   A0A226EEU3   A0A026VUK8   W5JGA9   U4TZ65   A0A224XE75   A0A0P4ZFV9   A0A158P3U7   B5U2W0   F4WKX6   F1CGQ2   F1CGQ3   G3GQP4   A0A0P5H1N4   A0A0P5J354   A0A0P6DSV9   A0A0P6F026   A0A0P5LAI0   A0A164XDX4   A0A0P6CM36   A0A0P6GSZ8   A0A0P5QER7   A0A0P5YT07   K7J1M8   A0A0P5S303   A0A151WSE3   A0A0P5PRB7   A0A084W007   R4FQA1   B8QQQ1   A0A1S4EMH0   F5HKL0   E9H0K2   A0A182SD58   A0A067XL24   A0A195CNZ7   A0A0L7LIA9   A0A195DZM2   A0A195ATJ4   A0A2H8TVY8   D6W808   A0A151K0E7   A0A182LGK1   A0A182UXQ5   A0A291C3B4   K7J0K5   A0A182Q6I6   J9JVY2   Q7M4I3   A0A1D2MGY3   A0A232F2M8   A0A348G5Y4   A0A0P6G9Q0   A0A1W6EW33   A0A2R7VPE0   A0A0J7KRY6   A0A1B6DCP0   A0A1B6E0S4   A0A2P2I6P9   A0A0P6I905  

EC Number

3.4.21.-

Pubmed

19121390    28756777    15944088    22118469    26354079    28410430    + More
28004739    24845553    20075255    20798317    26483478    17510324    21282665    28648823    27129103    24508170    30249741    20920257    23761445    23537049    21347285    20454652    22359676    21719571    24438588    12364791    14747013    17210077    21292972    26227816    18362917    19820115    20966253    27289101   

EMBL

AB073673    BAB91156.1    BABH01034383    BABH01034384    NWSH01000785    PCG74210.1    + More
KZ149929    PZC77442.1    AY061933    AAL31704.1    AY672793    AAV91015.1    AY672792    AAV91014.1    AY061935    AAL31706.1    AY061934    AAL31705.1    AGBW02010265    OWR48842.1    ODYU01011542    SOQ57348.1    KQ459589    KPI97653.1    AGBW02014657    OWR41265.1    AGBW02013393    OWR43495.1    OWR41269.1    KZ288206    PBC33104.1    KU668683    APA05228.1    OWR43494.1    KQ434875    KZC09758.1    NEVH01008287    PNF34238.1    PNF34237.1    KQ435944    KOX68265.1    FX985286    BAX07299.1    DS231877    EDS42128.1    GEZM01037396    JAV82073.1    KK852669    KDR18888.1    GL449438    EFN82690.1    JXUM01080533    KQ563191    KXJ74306.1    CH477234    EAT46744.1    KQ414596    KOC69988.1    GL767674    EFZ13255.1    NNAY01002080    OXU22140.1    GDKW01003671    JAI52924.1    GEDC01000715    JAS36583.1    ACPB03015962    LNIX01000004    OXA55910.1    KK107847    QOIP01000005    EZA47437.1    RLU23132.1    ADMH02001605    ETN61854.1    KB631792    ERL86132.1    GFTR01005660    JAW10766.1    GDIP01213944    JAJ09458.1    ADTU01008561    FJ159442    FJ159443    GL888206    EGI65218.1    HQ257242    ADY75779.1    HQ257243    ADY75780.1    JF979158    JF979159    AEN41590.1    GDIQ01233256    JAK18469.1    GDIQ01211809    JAK39916.1    GDIQ01073307    JAN21430.1    GDIQ01076108    GDIQ01067715    JAN27022.1    GDIQ01172773    JAK78952.1    LRGB01001005    KZS14121.1    GDIQ01090555    JAN04182.1    GDIQ01211810    GDIQ01076107    GDIQ01057117    GDIQ01049775    GDIQ01030295    JAN64442.1    GDIQ01136492    JAL15234.1    GDIP01053817    JAM49898.1    GDIQ01093048    JAL58678.1    KQ982783    KYQ50711.1    GDIQ01125343    JAL26383.1    ATLV01019066    KE525259    KFB43551.1    GAHY01000519    JAA76991.1    EU665686    ACF70480.1    AAAB01008859    EGK96762.1    GL732581    EFX74738.1    JX399874    AGI44422.1    KQ977513    KYN02207.1    JTDY01001069    KOB74971.1    KQ980044    KYN18074.1    KQ976746    KYM75330.1    GFXV01005523    MBW17328.1    KQ971307    EFA11581.1    KQ981292    KYN43137.1    KX950713    ATF27964.1    AXCN02000328    ABLF02021787    LJIJ01001282    ODM92260.1    NNAY01001198    OXU24769.1    FX985522    BBF97857.1    GDIQ01036590    JAN58147.1    KY563523    ARK19932.1    KK854015    PTY09392.1    LBMM01003785    KMQ93137.1    GEDC01013824    JAS23474.1    GEDC01005772    JAS31526.1    IACF01004094    LAB69689.1    GDIQ01007827    JAN86910.1   

Proteomes

UP000005204    UP000218220    UP000007151    UP000053268    UP000242457    UP000076502    + More
UP000005203    UP000235965    UP000053105    UP000192223    UP000002320    UP000027135    UP000002358    UP000008237    UP000069940    UP000249989    UP000008820    UP000053825    UP000215335    UP000015103    UP000198287    UP000053097    UP000279307    UP000000673    UP000030742    UP000005205    UP000007755    UP000076858    UP000075809    UP000030765    UP000079169    UP000007062    UP000000305    UP000075901    UP000078542    UP000037510    UP000078492    UP000078540    UP000007266    UP000078541    UP000075882    UP000075903    UP000075886    UP000007819    UP000094527    UP000036403   

PRIDE

Q8MP08    H9JGM1   

Pfam

PF00089   Trypsin        + More
PF12032   CLIP

Interpro

IPR033116   TRYPSIN_SER        + More
IPR022700   CLIP       
IPR038565   CLIP_sf       
IPR018114   TRYPSIN_HIS       
IPR001254   Trypsin_dom       
IPR009003   Peptidase_S1_PA       
IPR001314   Peptidase_S1A       

SUPFAM

SSF50494   SSF50494       

Gene 3D

2.20.20.90    

CDD

cd00190  Tryp_SPc

ProteinModelPortal

Q8MP08   H9JGM1   A0A2A4JS27   A0A2W1BQQ9   Q8I927   Q5MPB7   + More
Q5MPB8   Q8I925   Q8I926   A0A212F528   A0A2H1WWB5   A0A194PXA8   A0A212EIG1   A0A212EPS0   A0A212EIF7   A0A2A3ENM0   A0A1I9UVU8   A0A212EPV1   A0A154PEP1   A0A088ADM5   A0A2J7R078   A0A2J7R073   A0A0M8ZP71   A0A1V1FYF4   A0A1W4WNW1   A0A1W4WNW0   B0WB63   A0A1Y1M8P7   A0A067RFG3   K7J1M7   E2BNP2   A0A182GQG7   Q17J63   A0A0L7RGN5   E9J1L2   A0A1S4F0R9   A0A232EUU8   A0A0P4VP51   A0A1B6EF69   T1HGB7   A0A226EEU3   A0A026VUK8   W5JGA9   U4TZ65   A0A224XE75   A0A0P4ZFV9   A0A158P3U7   B5U2W0   F4WKX6   F1CGQ2   F1CGQ3   G3GQP4   A0A0P5H1N4   A0A0P5J354   A0A0P6DSV9   A0A0P6F026   A0A0P5LAI0   A0A164XDX4   A0A0P6CM36   A0A0P6GSZ8   A0A0P5QER7   A0A0P5YT07   K7J1M8   A0A0P5S303   A0A151WSE3   A0A0P5PRB7   A0A084W007   R4FQA1   B8QQQ1   A0A1S4EMH0   F5HKL0   E9H0K2   A0A182SD58   A0A067XL24   A0A195CNZ7   A0A0L7LIA9   A0A195DZM2   A0A195ATJ4   A0A2H8TVY8   D6W808   A0A151K0E7   A0A182LGK1   A0A182UXQ5   A0A291C3B4   K7J0K5   A0A182Q6I6   J9JVY2   Q7M4I3   A0A1D2MGY3   A0A232F2M8   A0A348G5Y4   A0A0P6G9Q0   A0A1W6EW33   A0A2R7VPE0   A0A0J7KRY6   A0A1B6DCP0   A0A1B6E0S4   A0A2P2I6P9   A0A0P6I905  

PDB

4DGJ     E-value=6.23819e-28,     Score=306

KEGG

692435    no KO assigned | (RefSeq) CLIP4, BmCLIP4, BmSP, Sp; clip domain serine protease 4

GO

GO:0004252   GO:0090729   GO:0005576   GO:0016504   GO:0046872   GO:0046658   GO:0019897   GO:0016021   GO:0005615   GO:0006508   GO:0016020   GO:0005515   GO:0042555   GO:0006260   GO:0004672   GO:0048384  

Subcellular location

Secreted  

Length:

234

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.01576

Exp number, first 60 AAs:

0

Total prob of N-in:

0.09146

outside

1  -  234

 

 

Pi

59.819166

Theta

48.291836

Tajima's D

-0.782408

CLR

1.131977

CSRT

0.181540922953852

Interpretation

Uncertain