SGID Silkworm Genome Informatics Database
Gene
KWMTBOMO04079  Validated by peptides from experiments
Pre Gene Modal
BGIBMGA008625
Annotation
PREDICTED:_DNA_replication_licensing_factor_Mcm2_isoform_X1_[Bombyx_mori]
Full name
DNA helicase       + More
DNA replication licensing factor Mcm2      
CTP synthase      
Alternative Name
Minichromosome maintenance 2 protein
UTP--ammonia ligase
Location in the cell
Nuclear   Reliability : 4.467
 

Sequence

CDS
ATGAGTTCTCCAATCCCAGACACACCTTCAGACCGCGAAGGAGCTCAATCACGAATGACATCACCAGCAAGAGAATATGAAATGTTTGAAGATGAGGGGGCTATACTCGGTGATAATCCTCTTGAGGAAGAAGAAGATGGAGAGGAACTTTTCAATGATAATATGGAAGCTGATTATCGTCCTATGCCCGCGCTGGATCGCTATGATAATGAAGACCTTGATGATGATGAATACGATGCAATGTCCATTCGAGACCGTGTGGCAGTTGAACAGGAGCTCCGAAGAAGAGATAGAGATGAAGGAAGAATAAGACGTGATGATCGTGATCTGTTGTACGATGAGTCGGATGATGAAAATGCCGATGCCCCTCGAGCGAAACGAAGGAGGGCTGCGGAGAATGCAGCGATGGGTGCAGAAGAACAAGTGGAAGAAGGTATCGAAAGTATTGAGAATTTAGAAGATACTAAAGGTTACACCACCAAGGAGTGGGTTTCAATGCTTGGACCTAGAACTGAAATTGCTAACAGGTTCAAGAACTTCCTTAGAACTTTCACAAATAACAAAGGCCAGTTTGTGTACAAGGACCGTATCAGACGCATGTGTGAGCATAACCAAGCATCCTTTCATGTTGAGTTTGATCTGTTAGCTCATAGAGAACAAGTTTTAGCCTATTTTTTGCCTGAAGCTCCCTTCCAGATGTTGCAAATATTCGATGAGGTGGCCAAAGAGATAGTCCTCCAAATATTCCCAAGCTACGAACGCGTGACCTCTGAGATCCACGTCCGCATATCAGACCTACCGCTCATTGAGGAACTTCGCACCTTCAGAAAACTTCACCTCAACCAGCTTGTGAGAACTGTTGGAGTGATAACGTCGACCACCGGAGTACTACCACAACTGTCCGTTGTTAAATACGATTGTAACCGTTGTGGATATATACTGGGACCATTCGTGCAGTCACAGAACTCTGAAGTCAAACCCGGGTCTTGTCCAGAGTGTCAGAGCTCTGGACCATTTATGGTGAATATGGAACAAACGGTGTATCGGAACTATCAAAAGGTCACCATCCAGGAGTCTCCAGGTCGGATCCCGGCCGGCAGGATACCTCGTAGCAAAGATGTCATATTACTGGCAGATTTGTGCGACCGATGTAAGCCCGGTGATGAAGTTGACTTGACCGGTGTATACACCAACAACTATGATGGCTCATTAAATACTGAACAGGGATTCCCAGTATTCGCGACAGTGATAATCGCGAATTACATCGTTGTGAAGGACTGCAAGCACATTGTTGAATCCTTGACCGATGAAGATGTAGCGAACATAGTCAAGTTGTCGAAGGATCCGAGAATCGGCGATAGAATAGTACAAAGCATAGCGCCTTCTATATACGGATACGATTACATTAAAAGGGGATTAGCTTTAGCGCTCTTTGGAGGAGAACCTAAAAATCCAGGCGAGAAACACAAAGTGAGAGGCGACATCAACGTTCTTATATGCGGGGACCCCGGAACAGCCAAGTCGCAGTTCCTGAAATATACTGAAAAGATAGCTCCCAGGGCCGTGTTCACGACCGGCCAGGGGGCCAGCGCCGTCGGTCTCACCGCCTACGTGCGGAAGAACCCAACCACTAGGTAA
Protein
MSSPIPDTPSDREGAQSRMTSPAREYEMFEDEGAILGDNPLEEEEDGEELFNDNMEADYRPMPALDRYDNEDLDDDEYDAMSIRDRVAVEQELRRRDRDEGRIRRDDRDLLYDESDDENADAPRAKRRRAAENAAMGAEEQVEEGIESIENLEDTKGYTTKEWVSMLGPRTEIANRFKNFLRTFTNNKGQFVYKDRIRRMCEHNQASFHVEFDLLAHREQVLAYFLPEAPFQMLQIFDEVAKEIVLQIFPSYERVTSEIHVRISDLPLIEELRTFRKLHLNQLVRTVGVITSTTGVLPQLSVVKYDCNRCGYILGPFVQSQNSEVKPGSCPECQSSGPFMVNMEQTVYRNYQKVTIQESPGRIPAGRIPRSKDVILLADLCDRCKPGDEVDLTGVYTNNYDGSLNTEQGFPVFATVIIANYIVVKDCKHIVESLTDEDVANIVKLSKDPRIGDRIVQSIAPSIYGYDYIKRGLALALFGGEPKNPGEKHKVRGDINVLICGDPGTAKSQFLKYTEKIAPRAVFTTGQGASAVGLTAYVRKNPTTR

Summary

Description
Acts as component of the Mcm2-7 complex (Mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the Mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.
Catalytic Activity
ATP + H2O = ADP + H(+) + phosphate
ATP + H2O + L-glutamine + UTP = ADP + CTP + 2 H(+) + L-glutamate + phosphate
Subunit
Component of the Mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order Mcm2-Mcm6-Mcm4-Mcm7-Mcm3-Mcm5 (Probable). Interacts with Mcm10.
Similarity
Belongs to the MCM family.
Belongs to the CTP synthase family.
Keywords
ATP-binding   Cell cycle   Cell division   Complete proteome   DNA replication   DNA-binding   Helicase   Hydrolase   Metal-binding   Nucleotide-binding   Nucleus   Phosphoprotein   Reference proteome   Zinc   Zinc-finger  
Feature
chain  DNA replication licensing factor Mcm2
EC Number
3.6.4.12
6.3.4.2
EMBL
NWSH01000139    PCG79153.1    ODYU01007344    SOQ50024.1    BABH01039421    BABH01039422    + More
BABH01039423    BABH01039424    BABH01039425    KQ459995    KPJ18533.1    KQ459600    KPI94294.1    JXJN01015972    KA648321    AFP62950.1    LJIG01022556    KRT79946.1    JRES01000936    KNC27056.1    GBXI01005942    JAD08350.1    GDHF01025324    JAI26990.1    CH902617    EDV44088.1    GEDC01026389    JAS10909.1    CH916377    EDV90520.1    ATLV01024815    KE525361    KFB51958.1    CH940650    EDW67538.1    GAMC01003180    JAC03376.1    KZ288206    PBC33109.1    CH964232    EDW80817.1    CM000160    EDW96488.1    CH954181    EDV49954.1    GBYB01011577    GBYB01014685    GBYB01014686    JAG81344.1    JAG84452.1    JAG84453.1    L42762    AE014297    AY069702    CH933806    EDW16691.1    CM000070    EAL27902.1    CP012526    ALC46999.1    KK107109    EZA59244.1    AXCN02000084    CH480815    EDW43062.1    QOIP01000008    RLU19179.1    OUUW01000007    SPP82812.1    GL446721    EFN87325.1    GFDL01015764    JAV19281.1    CCAG010004927    PYGN01002802    PSN29948.1    JXUM01088166    KQ563684    KXJ73497.1    CH477416    EAT41359.1    APCN01005029    AAAB01008880    EAA08670.5    JXUM01027084    KQ560777    KXJ80931.1    KQ977104    KYN05793.1    KQ979568    KYN20930.1    LBMM01006018    KMQ91014.1    ADMH02001267    ETN63293.1    KQ435824    KOX72070.1    ADTU01014870    DS231847    EDS36437.1    GL768221    EFZ11696.1    GL888818    EGI57938.1    GL439602    EFN66971.1    AXCM01000384    KQ414697    KOC63500.1    KK854039    PTY10046.1    KQ435037    KZC14122.1    KK852777    KDR16761.1    KQ982911    KYQ49335.1    KQ976662    KYM78597.1    ACPB03005149    GFTR01008220    JAW08206.1    GBBI01002389    JAC16323.1    KQ981864    KYN34214.1    GEBQ01016385    GEBQ01008553    JAT23592.1    JAT31424.1    KM036511    AIP98398.1    APGK01040543    KB740983    ENN76347.1    GECZ01028772    JAS40997.1    NEVH01014371    PNF27677.1    KB632410    ERL95212.1    CVRI01000004    CRK87607.1    GDHC01011151    JAQ07478.1    GBHO01024500    JAG19104.1    GBRD01004521    JAG61300.1    GBHO01035774    JAG07830.1    GDHC01006122    JAQ12507.1    GEZM01053963    JAV73893.1   
Pfam
PF12619   MCM2_N        + More
PF17855   MCM_lid
PF17207   MCM_OB
PF00493   MCM
PF14551   MCM_N
PF03153   TFIIA
PF00117   GATase
PF06418   CTP_synth_N
PF00005   ABC_tran
PF00067   p450
Interpro
IPR012340   NA-bd_OB-fold        + More
IPR001208   MCM_dom       
IPR031327   MCM       
IPR008045   MCM2       
IPR027417   P-loop_NTPase       
IPR033762   MCM_OB       
IPR041562   MCM_lid       
IPR027925   MCM_N       
IPR018525   MCM_CS       
IPR003593   AAA+_ATPase       
IPR004855   TFIIA_asu/bsu       
IPR009088   TFIIA_b-brl       
IPR017926   GATASE       
IPR029062   Class_I_gatase-like       
IPR004468   CTP_synthase       
IPR033828   GATase1_CTP_Synthase       
IPR017456   CTP_synthase_N       
IPR036396   Cyt_P450_sf       
IPR017871   ABC_transporter_CS       
IPR003439   ABC_transporter-like       
IPR001128   Cyt_P450       
IPR017972   Cyt_P450_CS       
SUPFAM
SSF50249   SSF50249        + More
SSF52540   SSF52540       
SSF50784   SSF50784       
SSF52317   SSF52317       
SSF48264   SSF48264       
Gene 3D
PDB
6HV9     E-value=2.51488e-114,     Score=1055

Ontologies

Topology

Subcellular location
Nucleus  
Length:
545
Number of predicted TMHs:
0
Exp number of AAs in TMHs:
0.7176
Exp number, first 60 AAs:
0
Total prob of N-in:
0.00065
outside
1  -  545
 
 

Population Genetic Test Statistics

Pi
313.260106
Theta
184.16132
Tajima's D
2.222395
CLR
0.092554
CSRT
0.916354182290885
Interpretation
Uncertain
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