Gene

KWMTBOMO04076  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA008621

Annotation

3-hydroxyacyl-CoA_dehydrogenase?_partial_[Agrotis_segetum]

Location

Bomo_Chr7(-):10829378-10833055      View in EpiBrowser

Full name

3-hydroxyacyl-CoA dehydrogenase type-2      

Alternative Name

17-beta-hydroxysteroid dehydrogenase 10
3-hydroxy-2-methylbutyryl-CoA dehydrogenase
3-hydroxyacyl-CoA dehydrogenase type II
Mitochondrial ribonuclease P protein 2
Scully protein
Type II HADH

Location in the cell

Mitochondrial   Reliability : 1.828

CDS

ATGCATAAGGGTATGGTCAGCCTGGTAACTGGTGGAGCTTCTGGGTTAGGTAGGGCTACAGCTGAGAGACTGGTGAAACAAGGCGGAAAGGTTGTTCTGCTAGATATACAAGGAACGAGACTTAAAGAAGTTACAAAAGAAATAGGGGACGATGCTGTAGCTGTGACGGGATCAATAACATCAGAGGATGATGTTAAAAAAGCACTGGACATAACCAAAGACAAATTTGGCAGACTTGATACACTAGTCAATTGCGCTGGACAGTCAATAACACATCAGATTTATAACTTTAATAAAGATTTACCCAGTACATTGGCAGATTTTACAAAAATTATCAATATCAACACTGTTGGAACATTCAACACAATTAGGTTATCGGCTGGACTGATTGGTAAGAATGAACCAGACGCTAATGGCCAAAGAGGAGTTATAGTAAATACGGCATCAACAATAGCATACGAAGGAGATATTGGTCAAGCTGCATATGCGGCATCAACTGCGGCAATAATAGGAATGACGTTACCGATTGCAAGGGATCTAGCTTCAAAAGGCATTCGAGTTGTTACCATAGCACCAGGTATATTTGACACGCCCCTAACGTCATATTTACCAGAGAAAATGAGGGAATTCATCAAGCGTATGACTCCATTCCCCTCGAGATTGGGCAAACCTGAGGAGTTTGCTCATCTCGTTACAACTATAGTTGAGAATCCAATGTTGAACGGAGAAGTCATAAGATTAGATGGTGCACAAAGGTGGTTTCCGCTTTGA

Protein

MHKGMVSLVTGGASGLGRATAERLVKQGGKVVLLDIQGTRLKEVTKEIGDDAVAVTGSITSEDDVKKALDITKDKFGRLDTLVNCAGQSITHQIYNFNKDLPSTLADFTKIININTVGTFNTIRLSAGLIGKNEPDANGQRGVIVNTASTIAYEGDIGQAAYAASTAAIIGMTLPIARDLASKGIRVVTIAPGIFDTPLTSYLPEKMREFIKRMTPFPSRLGKPEEFAHLVTTIVENPMLNGEVIRLDGAQRWFPL

Description

May function in mitochondrial tRNA maturation. Catalyzes the beta-oxidation at position 17 of androgens and estrogens, and has 3-alpha-hydroxysteroid dehydrogenase activity with androsterone. Catalyzes the third step in the beta-oxidation of fatty acids. Carries out oxidative conversions of 7-beta-hydroxylated bile acids. Also exhibits 20-beta-OH and 21-OH dehydrogenase activities with C21 steroids. Required for cell survival during embryonic development. May play a role in germline formation.

Catalytic Activity

a (3S)-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) + NADH
(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD(+) = 2-methyl-3-oxobutanoyl-CoA + H(+) + NADH
NAD(+) + testosterone = androst-4-ene-3,17-dione + H(+) + NADH
NADP(+) + testosterone = androst-4-ene-3,17-dione + H(+) + NADPH

Biophysicochemical Properties

33.7 uM for acetoacetyl-CoA (in the presence of 0.2 mM NADH, at pH 6.4 and 25 degrees Celsius)
101 uM for beta-hydroxybutyryl-CoA (in the presence of 1 mM NAD, at pH 9.3 and 25 degrees Celsius)
37.3 uM for androsterone (in the presence of 1 mM NAD, at pH 9.3 and 25 degrees Celsius)
12.3 uM for 5-alpha-dihydrotestosterone (in the presence of 0.2 mM NADH, at pH 6.4 and 25 degrees Celsius)
11.1 uM for 17-beta-estradiol (in the presence of 0.2 mM NADH, at pH 9.3 and 25 degrees Celsius)
9 uM for 5-alpha-pregnan-20-beta-ol-3-one (in the presence of 1 mM NAD, at pH 9.3 and 25 degrees Celsius)
3 uM for isoursodeoxycholic acid (in the presence of 1 mM NAD, at pH 9.3 and 25 degrees Celsius)
32.5 uM for NADH (in the presence of acetoacetyl-CoA, at pH 7.0 and 25 degrees Celsius)
64.4 uM for NAD (in the presence of beta-hydroxybutyryl-CoA, at pH 9.3 and 25 degrees Celsius)
124 uM for NAD (in the presence of aldosterone, at pH 9.3 and 25 degrees Celsius)

Subunit

Multimer.

Similarity

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Keywords

Complete proteome   Mitochondrion   NAD   Oxidoreductase   Reference proteome   tRNA processing  

Feature

chain  3-hydroxyacyl-CoA dehydrogenase type-2

Uniprot

H9JGH4   A0A068FMP4   A0A194PLU3   A0A3S2NNC5   A0A194RMH3   A0A212EJ30   + More
A0A2A4K558   B4Q2N6   B3NTA4   A0A1E1XB23   B4M6R1   A0A1W4UN75   O18404   R4V243   B4I6N3   A0A224Z8S0   A0A2C9JID1   L7MDN0   A0A0M4EZZ4   A0A3B0K0K0   A0A293MXH6   A0A0C9RY70   B3MXV0   B4L296   Q29H71   B4H078   D6WFE2   A0A1B0GJR4   A0A1W4XN13   G3MK41   A0A131YYH0   A0A1D2M829   B4JNB1   A0A0A1X8C9   A0A1L8DZJ3   B4NEB5   E3TDR7   A0A023FVV6   A0A131XC52   E3TDJ4   A0A2R5LN65   A0A069DY28   A0A0P4VTY5   R4FNB7   A0A034WG91   A0A0C9R451   U3GM45   T1PE05   A0A0K8UAH4   A0A0A9Z6D1   Q5XJS8   A0A067R1F2   A0A3B4C668   A0A1Z5L9R7   A0A1W4XXI0   W8C108   A0A023FLX5   A0A1A9XA65   A0A0K8RNJ1   M7AQR3   A0A1W4XJI9   A0A1I8P3Q2   A0A2D0SH21   A0A1B6LJU8   A0A1B6F172   A0A224XV14   A0A3B3C5E4   A0A1I8P3Q5   D3TLT8   I3KID3   A0A3S0ZQZ8   A0A1B6JZI7   A0A3B4H904   A0A023F9Q1   A0A0V0G444   A0A3P9DNA6   A0A3B4WFP0   A0A3B4TR48   A0A3B4WH98   A0A3B4TR38   A0A1A7XM58   U5EUU3   H3B6K3   A0A3Q3L724   A0A0L8HI08   A0A3Q2DZR4   A0A3B1KFP3   A0A3Q2Y663   A0A2M3ZCD3   A0A2M4BXJ7   A0A3Q2VAE6   A0A3P8PZC7   A0A182FA62   A0A3Q2VJS8   A0A3B3HLN0   A0A3P9IJ16   A0A0F8ASV8   A0A1L8EE35   A0A2M3ZCB0   A0A1Q3G3T0  

EC Number

1.1.1.35

Pubmed

19121390    26385554    26354079    22118469    17994087    17550304    + More
28503490    9585418    10731132    12537572    12537569    12917011    16979555    17924685    28797301    15562597    25576852    26131772    15632085    18362917    19820115    22216098    26830274    27289101    25830018    20634964    28049606    26334808    27129103    25348373    25315136    25401762    26823975    23594743    24845553    28528879    24495485    23624526    29451363    20353571    25186727    25474469    9215903    25329095    17554307    25835551   

EMBL

BABH01039421    KJ622101    AID66691.1    KQ459600    KPI94297.1    RSAL01000210    + More
RVE44222.1    KQ459995    KPJ18530.1    AGBW02014538    OWR41485.1    NWSH01000139    PCG79156.1    CM000162    EDX02677.1    CH954180    EDV46555.1    GFAC01002754    JAT96434.1    CH940653    EDW62478.1    Y15102    AE014298    AY121672    BT029045    BT132763    KC740963    AGM32787.1    CH480823    EDW55981.1    GFPF01011746    MAA22892.1    GACK01002974    JAA62060.1    CP012528    ALC49867.1    OUUW01000003    SPP78541.1    GFWV01019322    MAA44050.1    GBZX01000104    JAG92636.1    CH902630    EDV38565.1    CH933810    EDW07757.1    CH379064    EAL31887.1    CH479200    EDW29673.1    KQ971319    EFA00491.1    AJWK01022146    JO842242    AEO33859.1    GEDV01004318    JAP84239.1    LJIJ01002921    ODM89092.1    CH916371    EDV92204.1    GBXI01007369    JAD06923.1    GFDF01002382    JAV11702.1    CH964239    EDW82084.1    GU588497    ADO28453.1    GBBL01001701    JAC25619.1    GEFH01004639    JAP63942.1    GU588424    ADO28380.1    GGLE01006818    MBY10944.1    GBGD01002615    JAC86274.1    GDKW01000596    JAI55999.1    ACPB03014675    GAHY01000628    JAA76882.1    GAKP01005605    JAC53347.1    GBYB01001591    JAG71358.1    KF031120    AGT97387.1    KA646375    AFP61004.1    GDHF01028999    JAI23315.1    GBHO01003590    GBRD01011971    GBRD01011970    GDHC01009188    JAG40014.1    JAG53853.1    JAQ09441.1    CR735130    BC083219    BC165246    AAH83219.1    AAI65246.1    KK852996    KDR12703.1    GFJQ02002841    JAW04129.1    GAMC01003782    JAC02774.1    GBBK01001865    JAC22617.1    GADI01001659    JAA72149.1    KB575110    EMP26949.1    GEBQ01016088    JAT23889.1    GECZ01025777    JAS43992.1    GFTR01004176    JAW12250.1    CCAG010012474    EZ422390    ADD18666.1    AERX01043880    RQTK01000374    RUS80743.1    GECU01003373    JAT04334.1    GBBI01001059    JAC17653.1    GECL01003313    JAP02811.1    HADW01017781    HADX01009706    SBP19181.1    GANO01001305    JAB58566.1    AFYH01083165    AFYH01083166    AFYH01083167    AFYH01083168    AFYH01083169    AFYH01083170    AFYH01083171    KQ418094    KOF88861.1    GGFM01005367    MBW26118.1    GGFJ01008583    MBW57724.1    KQ040919    KKF33011.1    GFDG01001832    JAV16967.1    GGFM01005390    MBW26141.1    GFDL01000582    JAV34463.1   

Proteomes

UP000005204    UP000053268    UP000283053    UP000053240    UP000007151    UP000218220    + More
UP000002282    UP000008711    UP000008792    UP000192221    UP000000803    UP000001292    UP000076420    UP000092553    UP000268350    UP000007801    UP000009192    UP000001819    UP000008744    UP000007266    UP000092461    UP000192224    UP000094527    UP000001070    UP000007798    UP000221080    UP000015103    UP000095301    UP000000437    UP000027135    UP000261440    UP000092443    UP000031443    UP000192223    UP000095300    UP000261560    UP000092444    UP000005207    UP000271974    UP000261460    UP000265160    UP000261360    UP000261420    UP000008672    UP000261640    UP000053454    UP000265020    UP000018467    UP000264820    UP000264840    UP000265100    UP000069272    UP000001038    UP000265180    UP000265200   

PRIDE

H9JGH4    O18404    G3MK41    I3KID3   

Pfam

PF00106   adh_short

Interpro

IPR002347   SDR_fam        + More
IPR036291   NAD(P)-bd_dom_sf       
IPR020904   Sc_DH/Rdtase_CS       

SUPFAM

SSF51735   SSF51735       

ProteinModelPortal

H9JGH4   A0A068FMP4   A0A194PLU3   A0A3S2NNC5   A0A194RMH3   A0A212EJ30   + More
A0A2A4K558   B4Q2N6   B3NTA4   A0A1E1XB23   B4M6R1   A0A1W4UN75   O18404   R4V243   B4I6N3   A0A224Z8S0   A0A2C9JID1   L7MDN0   A0A0M4EZZ4   A0A3B0K0K0   A0A293MXH6   A0A0C9RY70   B3MXV0   B4L296   Q29H71   B4H078   D6WFE2   A0A1B0GJR4   A0A1W4XN13   G3MK41   A0A131YYH0   A0A1D2M829   B4JNB1   A0A0A1X8C9   A0A1L8DZJ3   B4NEB5   E3TDR7   A0A023FVV6   A0A131XC52   E3TDJ4   A0A2R5LN65   A0A069DY28   A0A0P4VTY5   R4FNB7   A0A034WG91   A0A0C9R451   U3GM45   T1PE05   A0A0K8UAH4   A0A0A9Z6D1   Q5XJS8   A0A067R1F2   A0A3B4C668   A0A1Z5L9R7   A0A1W4XXI0   W8C108   A0A023FLX5   A0A1A9XA65   A0A0K8RNJ1   M7AQR3   A0A1W4XJI9   A0A1I8P3Q2   A0A2D0SH21   A0A1B6LJU8   A0A1B6F172   A0A224XV14   A0A3B3C5E4   A0A1I8P3Q5   D3TLT8   I3KID3   A0A3S0ZQZ8   A0A1B6JZI7   A0A3B4H904   A0A023F9Q1   A0A0V0G444   A0A3P9DNA6   A0A3B4WFP0   A0A3B4TR48   A0A3B4WH98   A0A3B4TR38   A0A1A7XM58   U5EUU3   H3B6K3   A0A3Q3L724   A0A0L8HI08   A0A3Q2DZR4   A0A3B1KFP3   A0A3Q2Y663   A0A2M3ZCD3   A0A2M4BXJ7   A0A3Q2VAE6   A0A3P8PZC7   A0A182FA62   A0A3Q2VJS8   A0A3B3HLN0   A0A3P9IJ16   A0A0F8ASV8   A0A1L8EE35   A0A2M3ZCB0   A0A1Q3G3T0  

PDB

1U7T     E-value=1.7489e-76,     Score=725

KEGG

101744555    K08683 3-hydroxyacyl-CoA dehydrogenase / 3-hydroxy-2-methylbutyryl-CoA dehydrogenase [EC:1.1.1.35 1.

PATHWAY

00280    Valine, leucine and isoleucine degradation - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0047022   GO:0047035   GO:0008210   GO:0008205   GO:0004303   GO:0090646   GO:0008209   GO:0018454   GO:0030678   GO:0006631   GO:0006637   GO:0016491   GO:0047015   GO:0090502   GO:0005739   GO:0005829   GO:0016229   GO:0008202   GO:0003857   GO:0030283   GO:0035410   GO:0016020   GO:0009408   GO:0009409  

Subcellular location

Mitochondrion   Localizes to the lipid droplet fraction in early embryos.   With evidence from 4 publications.

Length:

256

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.48421

Exp number, first 60 AAs:

0.0064

Total prob of N-in:

0.04553

outside

1  -  256

 

 

Pi

282.60588

Theta

159.543585

Tajima's D

1.832725

CLR

0.000001

CSRT

0.854357282135893

Interpretation

Uncertain