Gene

KWMTBOMO04003  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA012780

Annotation

putative_succinyl-CoA_synthetase_small_subunit_[Danaus_plexippus]

Location

Bomo_Chr7(-):8089444-8095870      View in EpiBrowser

Full name

Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial      

Alternative Name

Succinyl-CoA synthetase subunit alpha
Succinyl-coenzyme A synthetase alpha subunit 1

Location in the cell

Mitochondrial   Reliability : 2.656

CDS

ATGGCTATGCCAGTCCGGGTTCTTTCTAAGTTCAAAGATGGACTAAAACTCGGCAATGTACGATTTGCATCCGGCAACCCTTATGCTGAAACCAGAAAAAATTTGATCCTTACAAGTGAAACAAAAGTGATTGTTCAGGGTTTCACTGGAAAGCAGGGTACCTTCCACAGCCAACAAGCCCTTGACTATGGTACTAAAGTTGTTGGAGGAGTGTCACCAAAGAAGGCTGGTACAGAACATCTTGGTAAGCCTGTGTTTGGTACAGTCAAAGAGGCAAAGGCAGGCACAGGAGCAACTGCCTCTGTTATTTATGTACCTCCCCCTGGAGCTGCAGCAGCCATTCTAGAAGCTATTGAGGCTGAAATGCCCCTCATTGTGTGCATTACAGAAGGTGTGCCACAACATGATATGGTGCGAGTAAAACATGCCCTCTTGAGACAAAACAAGTCTAGACTGGTTGGTCCTAACTGTCCTGGTATCATTGCTCCCGAGAAGTGTAAAATTGGTATCATGCCAGCTGCAGTGCACAAACGTGGTTGCATCGGAGTTGTATCGCGTTCTGGGACATTGACCTATGAAGCCTGTCATCAGACTACTATTACTGGCTTAGGCCAAACTTTGTGTGTCGGTATTGGAGGGGATCCCTTCAACGGAACAGACTTCATTGACTGCTTAGAAGTTTTCCTCAAGGATCCTGAGACCAAGGGCATCATCCTCATTGGTGAAATTGGTGGAAATGCTGAAGAATTAGCATCAGAATATTTGACTCAGTACAATACTGGTCAAAAAGCTAAGCCTGTGGTGTCGTTTATCGCCGGTTTGACGGCTCCCCCGGGTCGACGAATGGGTCACGCTGGTGCCATCATTTCGGGCGGCAAAGGAGGCGCCATGGACAAAATTAAGGCTTTAGAAAAAGCTAATGTAATCGTCACTCGGTCTCCGGCCAAAATGGGCGTGGAACTTCACAAAGAAATGAAACGATTAGAGATCATCTAA

Protein

MAMPVRVLSKFKDGLKLGNVRFASGNPYAETRKNLILTSETKVIVQGFTGKQGTFHSQQALDYGTKVVGGVSPKKAGTEHLGKPVFGTVKEAKAGTGATASVIYVPPPGAAAAILEAIEAEMPLIVCITEGVPQHDMVRVKHALLRQNKSRLVGPNCPGIIAPEKCKIGIMPAAVHKRGCIGVVSRSGTLTYEACHQTTITGLGQTLCVGIGGDPFNGTDFIDCLEVFLKDPETKGIILIGEIGGNAEELASEYLTQYNTGQKAKPVVSFIAGLTAPPGRRMGHAGAIISGGKGGAMDKIKALEKANVIVTRSPAKMGVELHKEMKRLEII

Description

Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and specificity for either ATP or GTP is provided by different beta subunits.

Catalytic Activity

ATP + CoA + succinate = ADP + phosphate + succinyl-CoA
CoA + GTP + succinate = GDP + phosphate + succinyl-CoA

Subunit

Heterodimer of an alpha and a beta subunit. Different beta subunits determine nucleotide specificity. Together with an ATP-specific beta subunit, forms an ADP-forming succinyl-CoA synthetase (A-SCS). Together with a GTP-specific beta subunit forms a GDP-forming succinyl-CoA synthetase (G-SCS).

Similarity

Belongs to the succinate/malate CoA ligase alpha subunit family.

Keywords

Complete proteome   Ligase   Mitochondrion   Nucleotide-binding   Reference proteome   Transit peptide   Tricarboxylic acid cycle  

Feature

chain  Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial

Uniprot

H9JTB8   A0A3S2TGU8   A0A212EIE7   S4PML6   A0A194RKT2   A0A194Q5R0   + More
E3UKP6   V5SHZ8   A0A2A4JTB3   A0A1L8E146   A0A0A1X3P7   Q1HR33   A0A023EPT6   A0A1Y9G8K1   A0A034W897   A0A0L0BQF0   A0A1I8Q4T6   A0A0K8VW67   A0A0K8TN56   A0A1I8JVE7   A0A1C7CZS7   A0A1I8JV33   A0A1I8JTE3   Q7PXA8   A0A1I8JUR8   A0A1L8EH99   A0A240PPQ4   A0A2C9GUM6   A0A1Y9HAB6   A0A1Y9IVV5   A0A2M4AC31   W5J816   A0A1Y9H2T8   W8BDF5   T1E7J0   A0A1I8M5I0   A0A1Q3G444   A0A2M3ZGR0   A0A240PKK7   V5GQY1   A0A1I8JW55   B3NFY4   A0A1J1IAG1   B4KY99   D3TRD7   A0A1B0AJC3   A0A1A9WLE1   A0A336K9C0   E2AUB5   B4N590   A0A0P4VP23   R4G324   A0A240PPA9   A0A336LUD5   B3M805   A0A069DSN1   A0A3B0KGQ0   B5DPQ3   B4GS06   A0A0M4ERI3   B4PH63   B4IZW2   A0A0A9XJW2   A0A0A9WL89   B4LDG6   W8C099   A0A182YBD5   A0A224XSF2   A0A0V0G6I8   A0A084VXV8   A0A0A9WN87   B4HTU6   A0A1W4W4Q4   B4QPY9   Q94522   A0A023F9Q6   A0A158P3V0   A0A0L7RGD4   E9J1L4   D6W7D6   A0A1B6MI96   A0A2J7QSL9   A0A1B6JRD9   A0A3L8DTP8   V9ID95   E0VG44   U5ELV2   A0A0L7LPY4   J3JZ14   A0A0P5JIU0   A0A0C9Q444   A0A151K0M7   A0A1B0GF61   A0A067QT32   A0A088ADV3   T1PCI4   A0A2A3E2W5   E2BNP4   R4WCU3   A0A1S3CX11  

EC Number

6.2.1.4
6.2.1.5

Pubmed

19121390    22118469    23622113    26354079    25830018    17204158    + More
17510324    24945155    26483478    25348373    26108605    26369729    20966253    12364791    14747013    17210077    20920257    23761445    24495485    25315136    17994087    20353571    20798317    27129103    26334808    15632085    17550304    25401762    26823975    25244985    24438588    22936249    10731132    12537572    12537569    10071211    28017724    25474469    21347285    21282665    18362917    19820115    30249741    20566863    26227816    22516182    23537049    24845553    23691247   

EMBL

BABH01004162    BABH01011634    BABH01011635    RSAL01000155    RVE45656.1    AGBW02014660    + More
OWR41259.1    GAIX01003490    JAA89070.1    KQ460075    KPJ17940.1    KQ459582    KPI98735.1    HM449920    ADO33055.1    KF647633    AHB50495.1    NWSH01000620    PCG75251.1    GFDF01001792    JAV12292.1    GBXI01009014    JAD05278.1    DQ440261    CH477408    ABF18294.1    EAT41527.1    JXUM01044656    GAPW01002568    KQ561409    JAC11030.1    KXJ78673.1    GAKP01007171    GAKP01007170    JAC51781.1    JRES01001517    KNC22315.1    GDHF01009188    JAI43126.1    GDAI01002020    JAI15583.1    APCN01000834    AAAB01008987    EAA01194.2    GFDG01000774    JAV18025.1    AXCM01001181    AXCN02001518    GGFK01005022    MBW38343.1    ADMH02002105    ETN59000.1    GAMC01011533    GAMC01011532    JAB95023.1    GAMD01003002    JAA98588.1    GFDL01000472    JAV34573.1    GGFM01007006    MBW27757.1    GALX01001917    JAB66549.1    CH954178    EDV50813.1    CVRI01000047    CRK97203.1    CH933809    EDW17678.1    EZ423989    ADD20265.1    UFQS01000194    UFQT01000194    UFQT01000732    SSX01091.1    SSX21471.1    SSX26785.1    GL442815    EFN62972.1    CH964101    EDW79529.1    GDKW01002388    JAI54207.1    ACPB03016273    GAHY01002066    JAA75444.1    SSX01090.1    SSX21470.1    SSX26784.1    CH902618    EDV39913.1    GBGD01002148    JAC86741.1    OUUW01000009    SPP84916.1    CH379069    EDY73560.1    CH479188    EDW40541.1    CP012525    ALC45080.1    CM000159    EDW93300.1    CH916366    EDV96734.1    GBHO01024526    GBRD01000206    GDHC01021949    JAG19078.1    JAG65615.1    JAP96679.1    GBHO01034392    GBHO01034389    GBRD01017667    GBRD01014819    JAG09212.1    JAG09215.1    JAG48160.1    CH940647    EDW68904.1    GAMC01011531    GAMC01011530    JAB95024.1    GFTR01005056    JAW11370.1    GECL01002409    JAP03715.1    ATLV01018208    KE525224    KFB42802.1    GBHO01034390    JAG09214.1    CH480817    EDW50367.1    CM000363    CM002912    EDX09109.1    KMY97424.1    AE014296    AY089549    Y09067    GBBI01001054    JAC17658.1    ADTU01008562    KQ414596    KOC69915.1    GL767674    EFZ13224.1    KQ971307    EFA11129.1    GEBQ01004331    JAT35646.1    NEVH01011876    PNF31578.1    GECU01005917    JAT01790.1    QOIP01000005    RLU23138.1    JR038527    AEY58299.1    DS235131    EEB12350.1    GANO01001260    JAB58611.1    JTDY01000368    KOB77500.1    APGK01047228    APGK01047229    APGK01047230    BT128495    KB741077    AEE63452.1    ENN74242.1    GDIQ01197769    JAK53956.1    GBYB01008803    JAG78570.1    KQ981292    KYN43135.1    CCAG010012763    KK853313    KDR08649.1    KA646462    AFP61091.1    KZ288416    PBC26057.1    GL449438    EFN82692.1    AK417178    BAN20393.1   

Proteomes

UP000005204    UP000283053    UP000007151    UP000053240    UP000053268    UP000218220    + More
UP000008820    UP000069940    UP000249989    UP000069272    UP000037069    UP000095300    UP000075903    UP000075882    UP000075902    UP000075840    UP000007062    UP000075900    UP000075885    UP000075883    UP000075886    UP000075920    UP000000673    UP000075884    UP000095301    UP000075880    UP000076407    UP000008711    UP000183832    UP000009192    UP000092445    UP000091820    UP000000311    UP000007798    UP000015103    UP000075881    UP000007801    UP000268350    UP000001819    UP000008744    UP000092553    UP000002282    UP000001070    UP000008792    UP000076408    UP000030765    UP000001292    UP000192221    UP000000304    UP000000803    UP000005205    UP000053825    UP000007266    UP000235965    UP000279307    UP000009046    UP000037510    UP000019118    UP000078541    UP000092444    UP000027135    UP000005203    UP000242457    UP000008237    UP000079169   

PRIDE

H9JTB8    Q94522    A0A088ADV3   

Pfam

PF02629   CoA_binding        + More
PF00549   Ligase_CoA
PF00453   Ribosomal_L20
PF03901   Glyco_transf_22

Interpro

IPR017440   Cit_synth/succinyl-CoA_lig_AS        + More
IPR016102   Succinyl-CoA_synth-like       
IPR005811   CoA_ligase       
IPR036291   NAD(P)-bd_dom_sf       
IPR005810   CoA_lig_alpha       
IPR033847   Citrt_syn/SCS-alpha_CS       
IPR003781   CoA-bd       
IPR005813   Ribosomal_L20       
IPR035566   Ribosomal_protein_L20_C       
IPR005599   GPI_mannosylTrfase       

SUPFAM

SSF51735   SSF51735        + More
SSF52210   SSF52210       
SSF74731   SSF74731       

Gene 3D

3.40.50.261     1.10.1900.20    

CDD

cd07026  Ribosomal_L20

ProteinModelPortal

H9JTB8   A0A3S2TGU8   A0A212EIE7   S4PML6   A0A194RKT2   A0A194Q5R0   + More
E3UKP6   V5SHZ8   A0A2A4JTB3   A0A1L8E146   A0A0A1X3P7   Q1HR33   A0A023EPT6   A0A1Y9G8K1   A0A034W897   A0A0L0BQF0   A0A1I8Q4T6   A0A0K8VW67   A0A0K8TN56   A0A1I8JVE7   A0A1C7CZS7   A0A1I8JV33   A0A1I8JTE3   Q7PXA8   A0A1I8JUR8   A0A1L8EH99   A0A240PPQ4   A0A2C9GUM6   A0A1Y9HAB6   A0A1Y9IVV5   A0A2M4AC31   W5J816   A0A1Y9H2T8   W8BDF5   T1E7J0   A0A1I8M5I0   A0A1Q3G444   A0A2M3ZGR0   A0A240PKK7   V5GQY1   A0A1I8JW55   B3NFY4   A0A1J1IAG1   B4KY99   D3TRD7   A0A1B0AJC3   A0A1A9WLE1   A0A336K9C0   E2AUB5   B4N590   A0A0P4VP23   R4G324   A0A240PPA9   A0A336LUD5   B3M805   A0A069DSN1   A0A3B0KGQ0   B5DPQ3   B4GS06   A0A0M4ERI3   B4PH63   B4IZW2   A0A0A9XJW2   A0A0A9WL89   B4LDG6   W8C099   A0A182YBD5   A0A224XSF2   A0A0V0G6I8   A0A084VXV8   A0A0A9WN87   B4HTU6   A0A1W4W4Q4   B4QPY9   Q94522   A0A023F9Q6   A0A158P3V0   A0A0L7RGD4   E9J1L4   D6W7D6   A0A1B6MI96   A0A2J7QSL9   A0A1B6JRD9   A0A3L8DTP8   V9ID95   E0VG44   U5ELV2   A0A0L7LPY4   J3JZ14   A0A0P5JIU0   A0A0C9Q444   A0A151K0M7   A0A1B0GF61   A0A067QT32   A0A088ADV3   T1PCI4   A0A2A3E2W5   E2BNP4   R4WCU3   A0A1S3CX11  

PDB

6G4Q     E-value=8.72438e-101,     Score=936

KEGG

101735810    K01899 succinyl-CoA synthetase alpha subunit [EC:6.2.1.4 6.2.1.5] | (RefSeq) succinyl-CoA ligase [AD

PATHWAY

00020    Citrate cycle (TCA cycle) - Bombyx mori (domestic silkworm)
00640    Propanoate metabolism - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)
01200    Carbon metabolism - Bombyx mori (domestic silkworm)

GO

GO:0004775   GO:0004776   GO:0000166   GO:0005739   GO:0048037   GO:0006099   GO:0016874   GO:0006104   GO:0004550   GO:0006105   GO:0009142   GO:0005829   GO:0004778   GO:0016746   GO:0003735   GO:0019843   GO:0005840   GO:0006412   GO:0045244   GO:0003824   GO:0016021   GO:0016757   GO:0008152   GO:0008270   GO:0008892   GO:0006811   GO:0006032   GO:0004672   GO:0048384  

Subcellular location

Mitochondrion  

Length:

331

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.0368300000000001

Exp number, first 60 AAs:

0

Total prob of N-in:

0.02115

outside

1  -  331

 

 

Pi

231.087655

Theta

178.321308

Tajima's D

1.082502

CLR

0.079842

CSRT

0.68341582920854

Interpretation

Uncertain