Silkworm Genome Informatics Database

Gene

KWMTBOMO03960

Annotation

ecdysone_20-hydroxylase_[Bombyx_mori]

Location

Bomo_Chr7(+):7023114-7024468 View in EpiBrowser

Full name

Ecdysone 20-monooxygenase

Alternative Name

CYPCCCXIVA1
Cytochrome P450 314a1, mitochondrial
Protein shade

Location in the cell

Mitochondrial Reliability : 1.963

CDS

ATGATTTTGAGAGCAAGAAACGCTCGAAGAGTTCTAGCCAACAGTTTGGTGTTTCTTCTGTACCTACTGAGTGGACGTCCAGATTGGCAAAGACAAATTAATTCAGAACTACCACCGTATACAATGCTTTGCTCTGAAGATTTGGCAGGAGCACCTTCGGTCAGAGCTGCGATCAATGAAGCCTTTAGACTATTGCCTACAGCTCCATTTCTTGCACGGCTCTTAGATTCACCGATGACGATTGGAGGACACAAGATCCCGCCTGGAACGTTTGTGCTCGCCCATACGGCTGCGGCTTGTCGTAGAGAAGAAAACTTCTGGCGCGCGGAGGAATATCTTCCGGAGCGATGGATTAAAGTGCAGGAGCCGCACGCTTACTCGCTAGTGGCTCCATTCGGACGCGGACGCAGGATGTGTCCAGGGAAACGATTTGTAGAACTCGAACTACATTTGTTACTCGCAAAGGTGATTGTTTTTTTTTTCCTACCTATTCGCTGA

Protein

MILRARNARRVLANSLVFLLYLLSGRPDWQRQINSELPPYTMLCSEDLAGAPSVRAAINEAFRLLPTAPFLARLLDSPMTIGGHKIPPGTFVLAHTAAACRREENFWRAEEYLPERWIKVQEPHAYSLVAPFGRGRRMCPGKRFVELELHLLLAKVIVFFFLPIR

Description

Required for CNS development; midline glial cells. Involved in the metabolism of insect hormones; responsible for all ecdysone 20-monooxygenase activity during embryonic, larval and adult stages. May be involved in the breakdown of synthetic insecticides.

Catalytic Activity

AH2 + ecdysone + O2 = 20-hydroxyecdysone + A + H2O

Cofactor

heme

Miscellaneous

Member of the Halloween gene group.

Similarity

Belongs to the cytochrome P450 family.

Keywords

Alternative splicing Complete proteome Heme Iron Membrane Metal-binding Mitochondrion Monooxygenase Oxidoreductase Reference proteome Transit peptide

Feature

chain Ecdysone 20-monooxygenase
splice variant In isoform C.

Uniprot

L0N4N9 L0N5I8 Q3LFR2 Q2EMR3 S6CBA3 A0A0P0I9N6 + More
D2DEB0 A0A248QFG1 A0A2W1BUM3 A0A2A4J8X4 A0A1V0D9F0 A0A194R0I2 A0A212EJE2 A0A3S2NV91 A0A2H8TX85 A0A0J7NWX0 A0A3G1ZJK6 A0A1W6L1H2 J9K5U5 E0VHL8 E2AC01 E9JB01 A0A0C9R0C0 A0A0C9R3M8 A0A2J7QGF4 X1XDF6 A0A2J7QGG0 A0A026WTA0 A0A1I9W2S4 A0A0K0LB70 A0A067RDA3 A0A1S3DTS2 R9UA55 A0A3L8D9G5 M9SV74 U5KSD0 A0A154P5K5 M9SV71 A0A0C5BXD2 Q16RI4 A0A1Q1NKZ6 A0A023I730 Q306I2 K4JQD8 A0A088A312 A0A2R7WJE3 A0A0F7J1S1 A0A182P729 A0A023F626 A0A310SMY1 A0A0L7QXG8 Q563C4 E2B6K1 K7J971 J9KA32 A0A182UXD2 B0W3J4 A0A0N0BJD8 A0A182HT41 A0A182LF83 A0A182HT42 A0A182XX08 A0A1S4J4E5 A0A182R9W9 T1HSC5 A0A182KBD2 A0A182G832 W5J5A5 A0A182XI58 Q7QC35 H9MFM9 A0A0F7KYF0 A0A182J6S4 A0A182VX95 A0A2P8Y5W6 A0A0Q9WJA8 A0A182NFG1 A0A2L1DHD6 A0A1W4UN16 A0A0K2VFD3 A0A182FPI5 A0A1L8E416 A0A182QFH3 Q9VUF8-2 A0A0J9RWE8 A0A0R1DXS6 A0A1W4ULA8 A0A0A9XMH1 A0A0J9RVI8 B4QK08 B4HHR3 M9PI59 Q9VUF8 A0A336MFD5 B4PIE9 A0A2A3EAW2

EC Number

1.14.99.22

Pubmed

18226571 16473459 19682519 28881445 28756777 26354079 + More
22118469 20566863 20798317 21282665 24508170 24845553 30249741 24055487 17510324 28092127 17069637 24324548 25942333 25474469 15804580 20075255 20966253 25244985 26483478 20920257 23761445 12364791 14747013 17210077 19060216 22465741 24438588 29403074 17994087 29401467 14610274 10731132 12537572 12537569 12676319 22936249 17550304 25401762 12537568 12537573 12537574 16110336 17569856 17569867

EMBL

AK289332 BAM73859.1 AK289331 BAM73858.1 AB236417 BAE45332.1 + More
DQ372988 ABD18735.1 AB649121 BAN66315.2 KP764856 ALJ84054.1 FJ501968 ACM66924.1 KX443477 ASO98052.1 KZ149930 PZC77365.1 NWSH01002306 PCG68567.1 KY212068 ARA91632.1 KQ460930 KPJ10755.1 AGBW02014474 OWR41622.1 RSAL01000161 RVE45412.1 GFXV01006715 MBW18520.1 LBMM01001105 KMQ96915.1 MG745777 AYL99849.1 KX609541 ARN17948.1 ABLF02025371 ABLF02025372 DS235171 EEB12904.1 GL438389 EFN69019.1 GL770707 EFZ09995.1 GBYB01001435 GBYB01001436 GBYB01001849 JAG71202.1 JAG71203.1 JAG71616.1 GBYB01001431 GBYB01001434 JAG71198.1 JAG71201.1 NEVH01014371 PNF27670.1 ABLF02021856 PNF27668.1 KK107111 EZA58896.1 KU928172 APA21471.1 KM216996 KM216997 AIW79960.1 KK852777 KDR16753.1 JX462958 AGN52751.1 QOIP01000011 RLU16976.1 KC701466 AGI92301.1 KC579461 AGU16450.1 KQ434809 KZC06410.1 KC701461 AGI92296.1 KP001113 AJN91158.1 CH477709 EAT37005.2 KX660711 AQM57050.1 KC579456 AGU16445.1 DQ244074 ABB36784.1 JX566821 AFU86480.1 KK854838 PTY19140.1 KP899602 AKH03534.1 GBBI01002062 JAC16650.1 KQ761039 OAD58368.1 KQ414704 KOC63313.1 AY947552 AAX85208.1 GL445975 EFN88693.1 AAZX01002215 ABLF02035037 DS231832 EDS31780.1 KQ435719 KOX78791.1 APCN01000411 ACPB03019876 JXUM01047181 KQ561509 KXJ78352.1 ADMH02002069 ETN59657.1 EF546765 AAAB01008859 ABU42525.1 EAA08077.4 JN982228 AEX56134.1 KP942351 AKH45319.1 PYGN01000894 PSN39633.1 CH940647 KRF84793.1 MF598469 AVD30014.1 HACA01031265 CDW48626.1 GFDF01000594 JAV13490.1 AXCN02000214 AF484414 AE014296 AY089309 CM002912 KMY99594.1 CM000159 KRK01888.1 GBHO01023596 JAG20008.1 KMY99592.1 KMY99595.1 CM000363 EDX10446.1 CH480815 EDW41478.1 AGB94536.1 UFQS01000835 UFQT01000835 SSX07160.1 SSX27503.1 EDW94506.2 KZ288322 PBC28201.1

Proteomes

UP000218220 UP000053240 UP000007151 UP000283053 UP000036403 UP000007819 + More
UP000009046 UP000000311 UP000235965 UP000053097 UP000027135 UP000079169 UP000279307 UP000076502 UP000008820 UP000005203 UP000075885 UP000053825 UP000008237 UP000002358 UP000075903 UP000002320 UP000053105 UP000075840 UP000075882 UP000076408 UP000075900 UP000015103 UP000075881 UP000069940 UP000249989 UP000000673 UP000076407 UP000007062 UP000075880 UP000075920 UP000245037 UP000008792 UP000075884 UP000192221 UP000069272 UP000075886 UP000000803 UP000002282 UP000000304 UP000001292 UP000242457

PRIDE

L0N4N9 L0N5I8 Q3LFR2 Q9VUF8

Pfam

PF00067 p450 + More
PF03109 ABC1

Interpro

IPR036396   Cyt_P450_sf        + More
IPR017972   Cyt_P450_CS
IPR001128   Cyt_P450
IPR002401   Cyt_P450_E_grp-I
IPR011009   Kinase-like_dom_sf
IPR000719   Prot_kinase_dom
IPR004147   UbiB_dom

SUPFAM

SSF48264 SSF48264 + More
SSF56112 SSF56112

Gene 3D

1.10.630.10

ProteinModelPortal

L0N4N9 L0N5I8 Q3LFR2 Q2EMR3 S6CBA3 A0A0P0I9N6 + More
D2DEB0 A0A248QFG1 A0A2W1BUM3 A0A2A4J8X4 A0A1V0D9F0 A0A194R0I2 A0A212EJE2 A0A3S2NV91 A0A2H8TX85 A0A0J7NWX0 A0A3G1ZJK6 A0A1W6L1H2 J9K5U5 E0VHL8 E2AC01 E9JB01 A0A0C9R0C0 A0A0C9R3M8 A0A2J7QGF4 X1XDF6 A0A2J7QGG0 A0A026WTA0 A0A1I9W2S4 A0A0K0LB70 A0A067RDA3 A0A1S3DTS2 R9UA55 A0A3L8D9G5 M9SV74 U5KSD0 A0A154P5K5 M9SV71 A0A0C5BXD2 Q16RI4 A0A1Q1NKZ6 A0A023I730 Q306I2 K4JQD8 A0A088A312 A0A2R7WJE3 A0A0F7J1S1 A0A182P729 A0A023F626 A0A310SMY1 A0A0L7QXG8 Q563C4 E2B6K1 K7J971 J9KA32 A0A182UXD2 B0W3J4 A0A0N0BJD8 A0A182HT41 A0A182LF83 A0A182HT42 A0A182XX08 A0A1S4J4E5 A0A182R9W9 T1HSC5 A0A182KBD2 A0A182G832 W5J5A5 A0A182XI58 Q7QC35 H9MFM9 A0A0F7KYF0 A0A182J6S4 A0A182VX95 A0A2P8Y5W6 A0A0Q9WJA8 A0A182NFG1 A0A2L1DHD6 A0A1W4UN16 A0A0K2VFD3 A0A182FPI5 A0A1L8E416 A0A182QFH3 Q9VUF8-2 A0A0J9RWE8 A0A0R1DXS6 A0A1W4ULA8 A0A0A9XMH1 A0A0J9RVI8 B4QK08 B4HHR3 M9PI59 Q9VUF8 A0A336MFD5 B4PIE9 A0A2A3EAW2

PDB

3K9Y E-value=3.09742e-22, Score=254

KEGG

100127102 K10723 ecdysone 20-monooxygenase [EC:1.14.99.22] | (RefSeq) Cyp314a1; ecdysone 20-hydroxylase

PATHWAY

00981 Insect hormone biosynthesis - Bombyx mori (domestic silkworm)

GO

GO:0004497 GO:0020037 GO:0016021 GO:0016705 GO:0005506 GO:0004501 GO:0046872 GO:0005524 GO:0004672 GO:0008258 GO:0031966 GO:0007417 GO:0007295 GO:0007391 GO:0007494 GO:0045456 GO:0005739 GO:0035074 GO:0007298 GO:0005634 GO:0005665 GO:0043565 GO:0048384 GO:0055114

Subcellular location

Mitochondrion membrane

Length:

165

Number of predicted TMHs:

1

Exp number of AAs in TMHs:

15.85589

Exp number, first 60 AAs:

0.23047

Total prob of N-in:

0.87072

inside

1 - 143

TMhelix

144 - 164

outside

165 - 165

Pi

270.337109

Theta

137.204007

Tajima's D

3.038233

CLR

0.49092

CSRT

0.980950952452377

Interpretation

Uncertain

Sequence

Summary

Ontologies

Topology

Population Genetic Test Statistics