Silkworm Genome Informatics Database

Gene

KWMTBOMO03959

Pre Gene Modal

BGIBMGA010239

Annotation

ecdysone_20-hydroxylase_[Bombyx_mori]

Location

Bomo_Chr7(+):7010944-7022029 View in EpiBrowser

Full name

Ecdysone 20-monooxygenase

Alternative Name

CYPCCCXIVA1
Cytochrome P450 314a1, mitochondrial
Protein shade

Location in the cell

PlasmaMembrane Reliability : 2.808

CDS

ATGAGACCGAGCCATATTGTGATGCAGAGTACATCTCCTCCTCTGCTGGACTGGTCGTGTGTGCCGACGCTGGTGCTCGCCGTGATCGCAGTGGTTGTAGCTGTAACCGCGTTGCTAACGAGAACATCGGATGCTAAGCACTCGTGTCGTCTACCCGGCCCTCAGCCTCTTCCCTTCCTGGGAACCAGGTGGCTATTCTGGAGTAGATACAAAATGAATAAACTGCACGAGGCTTATGCAGATATGTTCAAGCGTTATGGGCCAGTGTTCATGGAGACGACGCCAGGTGGTGTCGCCGTGGTGTCGATAGCCGAGCGGACTGCTCTTGAGGCCGTACTGCGTTCTCCTGCTAAGAAACCTTACCGTCCGCCCACTGAGATCGTGCAAATGTACCGCAGAAGCAGACCAGACAGATACGCATCAACTGGACTCGTCAATGAGCAGGGAGAAAAATGGTACCACTTACGGCGCAACTTGACTACTGATCTAACGAGTCCACATACAATGCAAAATTTCCTTCCCCAATTGAACACCATCTCCGATGACTTCTTGGAGCTACTGAATACTTCTCGGCAGTCTGATGGCACAGTATACGCCTTCGAACAGCTGACAAACAGAATGGGTCTGGAATCGGTATGCGGTCTGATGCTGGGTTCAAGACTTGGGTTTCTCGAACGATGGATGTCGGGACGTGCAATGGCTCTGGCTGCTGCTGTCAAGAATCACTTTCGAGCACAGAGGGACTCTTATTACGGTGCTCCGCTTTGGAAATTTGCTCCTACAGCGCTCTACAAGACATTCGTTAAAAGCGAGGAAACTATACACGCGATAGTATCGGAGCTGATGGAGGAGGCAAAATCTAAAACCACTGGTATGGCCCAAGACGAGGCGATACAAGAGATTTTCCTCAAGATACTGGAAAACCCAGCACTGGACATGCGCGACAAGAAGGCTGCCATTATAGACTTCATAACTGCCGGTATTGAGACGGTAATTATTGTTGAATCGCTTTCTTGTCTTAAATATTGTTTGTCTTTATTAATATTTGTCTAA

Protein

MRPSHIVMQSTSPPLLDWSCVPTLVLAVIAVVVAVTALLTRTSDAKHSCRLPGPQPLPFLGTRWLFWSRYKMNKLHEAYADMFKRYGPVFMETTPGGVAVVSIAERTALEAVLRSPAKKPYRPPTEIVQMYRRSRPDRYASTGLVNEQGEKWYHLRRNLTTDLTSPHTMQNFLPQLNTISDDFLELLNTSRQSDGTVYAFEQLTNRMGLESVCGLMLGSRLGFLERWMSGRAMALAAAVKNHFRAQRDSYYGAPLWKFAPTALYKTFVKSEETIHAIVSELMEEAKSKTTGMAQDEAIQEIFLKILENPALDMRDKKAAIIDFITAGIETVIIVESLSCLKYCLSLLIFV

Description

Required for CNS development; midline glial cells. Involved in the metabolism of insect hormones; responsible for all ecdysone 20-monooxygenase activity during embryonic, larval and adult stages. May be involved in the breakdown of synthetic insecticides.

Catalytic Activity

AH2 + ecdysone + O2 = 20-hydroxyecdysone + A + H2O

Cofactor

heme

Miscellaneous

Member of the Halloween gene group.

Similarity

Belongs to the cytochrome P450 family.

Keywords

Alternative splicing Complete proteome Heme Iron Membrane Metal-binding Mitochondrion Monooxygenase Oxidoreductase Reference proteome Transit peptide

Feature

chain Ecdysone 20-monooxygenase
splice variant In isoform C.

Uniprot

Q3LFR2 Q2EMR3 L0N5I8 S6CBA3 A0A0P0I9N6 A0A2W1BUM3 + More
A0A2A4J8X4 A0A248QFG1 D2DEB0 A0A212EJE2 A0A1V0D9F0 A0A194Q2Z0 A0A0K8TT59 A0A1W4U9W6 A0A0R1DY93 A0A1W4ULA8 A0A0Q9XDI0 A0A0J9RVI8 B4HHR3 B4QK08 A0A0Q5U4F8 M9PI59 Q9VUF8 A0A2J7QGF4 B4PIE9 A0A2J7QGG0 B3M3F4 R9UA55 T1HSC5 B4KVM7 B4IWT4 A0A336MAY2 B4LEW8 A0A3B0JL24 A0A2S2Q0C8 Q2LZS4 A0A0R3P4A0 A0A0R3P476 A0A1Q1NKZ6 A0A0M4F155 B3ND40 A0A1B6EHD4 A0A1I8MBY6 K4JQD8 B4NM34 A0A1I9W2S4 A0A0K0LB70 E0VHL8 A0A2P8Y5W6 A0A1B6FWG4 M9SV74 U5KSD0 A0A0M3QWR1 M9SV71 A0A1I8Q469 A0A146KXT8 A0A0A9XMH1 A0A023I730 A0A1A9VHQ2 A0A1B0AFX2 A0A0A1WTZ6 A0A224XR57 A0A1A9XC80 A0A1B0AKY9 A0A1B6J7Y8 A0A1A9W8F7 A0A023F626 K9LCM6 A0A0K8VUZ4 A0A1B0G8W1 A0A034W759 A0A2H8TX85 J9K5U5 A0A0L0C4U9 A0A3G1ZJK6 A0A1B0DGD6 A0A1L8E416 E9JB01 A0A1B0CKE9 A0A0C9R3M8 E2AC01 A0A2S2QKE6 A0A182G832 A0A1S4J4E5 A0A0N0BJD8 A0A0C5BXD2 A0A067RDA3 J9KA32 Q563C4 A0A182MPH0 B0W3J4 A0A182VX95 A0A0T6AY57 K0GJA0 B4H842

EC Number

1.14.99.22

Pubmed

18226571 16473459 28756777 28881445 19682519 22118469 + More
26354079 26369729 17994087 17550304 22936249 10731132 12537568 12537572 12537573 12537574 16110336 17569856 17569867 14610274 12537569 12676319 18057021 15632085 28092127 25315136 24324548 20566863 29403074 24055487 26823975 25401762 25830018 25474469 25348373 26108605 21282665 20798317 26483478 24845553 15804580 23019649

EMBL

AB236417 BAE45332.1 DQ372988 ABD18735.1 AK289331 BAM73858.1 + More
AB649121 BAN66315.2 KP764856 ALJ84054.1 KZ149930 PZC77365.1 NWSH01002306 PCG68567.1 KX443477 ASO98052.1 FJ501968 ACM66924.1 AGBW02014474 OWR41622.1 KY212068 ARA91632.1 KQ459579 KPI99369.1 GDAI01000049 JAI17554.1 CM000159 KRK01887.1 CH933809 KRG06656.1 CM002912 KMY99592.1 KMY99595.1 CH480815 EDW41478.1 CM000363 EDX10446.1 CH954178 KQS43960.1 AE014296 AGB94536.1 AF484414 AY089309 NEVH01014371 PNF27670.1 EDW94506.2 PNF27668.1 CH902618 EDV39215.1 KPU77942.1 JX462958 AGN52751.1 ACPB03019876 EDW19498.2 KRG06659.1 CH916366 EDV97335.1 UFQS01000835 UFQT01000835 SSX07159.1 SSX27502.1 CH940647 EDW70225.2 OUUW01000002 SPP76070.1 GGMS01002024 MBY71227.1 CH379069 EAL29432.3 KRT07976.1 KRT07975.1 KX660711 AQM57050.1 CP012525 ALC44595.1 ALC45040.1 EDV51762.2 GEDC01000004 JAS37294.1 JX566821 AFU86480.1 CH964278 EDW85402.1 KU928172 APA21471.1 KM216996 KM216997 AIW79960.1 DS235171 EEB12904.1 PYGN01000894 PSN39633.1 GECZ01024652 GECZ01015222 JAS45117.1 JAS54547.1 KC701466 AGI92301.1 KC579461 AGU16450.1 ALC44593.1 KC701461 AGI92296.1 GDHC01017385 JAQ01244.1 GBHO01023596 JAG20008.1 KC579456 AGU16445.1 GBXI01011743 JAD02549.1 GFTR01005967 JAW10459.1 JXJN01029347 GECU01032662 GECU01012428 JAS75044.1 JAS95278.1 GBBI01002062 JAC16650.1 JF835074 JQ229645 AFH54210.1 AFI99108.1 GDHF01009621 JAI42693.1 CCAG010010271 GAKP01009349 JAC49603.1 GFXV01006715 MBW18520.1 ABLF02025371 ABLF02025372 JRES01000903 KNC27383.1 MG745777 AYL99849.1 AJVK01059538 GFDF01000594 JAV13490.1 GL770707 EFZ09995.1 AJWK01016155 GBYB01001431 GBYB01001434 JAG71198.1 JAG71201.1 GL438389 EFN69019.1 GGMS01008960 MBY78163.1 JXUM01047181 KQ561509 KXJ78352.1 KQ435719 KOX78791.1 KP001113 AJN91158.1 KK852777 KDR16753.1 ABLF02035037 AY947552 AAX85208.1 AXCM01008923 AXCM01008924 DS231832 EDS31780.1 LJIG01022630 KRT79543.1 JX066977 JX066978 JX066979 JX066980 JX066981 JX066982 JX066983 JX066984 JX066985 JX066986 JX066987 JX066988 JX066989 JX066990 JX066991 JX066992 JX066993 JX066994 JX066995 JX066996 JX066997 JX066998 JX066999 JX067000 JX067001 JX067002 JX067003 JX067004 JX067005 JX067006 JX067007 JX067008 JX067009 JX067010 AFU24662.1 AFU24663.1 AFU24664.1 AFU24665.1 AFU24666.1 AFU24667.1 AFU24668.1 AFU24669.1 AFU24670.1 AFU24671.1 AFU24672.1 AFU24673.1 AFU24674.1 AFU24675.1 AFU24676.1 AFU24677.1 AFU24678.1 AFU24679.1 AFU24680.1 AFU24681.1 AFU24682.1 AFU24683.1 AFU24684.1 AFU24685.1 AFU24686.1 AFU24687.1 AFU24688.1 AFU24689.1 AFU24690.1 AFU24691.1 AFU24692.1 AFU24693.1 AFU24694.1 AFU24695.1 CH479220 EDW34836.1

Proteomes

UP000218220 UP000007151 UP000053268 UP000192221 UP000002282 UP000009192 + More
UP000001292 UP000000304 UP000008711 UP000000803 UP000235965 UP000007801 UP000015103 UP000001070 UP000008792 UP000268350 UP000001819 UP000092553 UP000095301 UP000007798 UP000009046 UP000245037 UP000095300 UP000078200 UP000092445 UP000092443 UP000092460 UP000091820 UP000092444 UP000007819 UP000037069 UP000092462 UP000092461 UP000000311 UP000069940 UP000249989 UP000053105 UP000027135 UP000075883 UP000002320 UP000075920 UP000008744

PRIDE

Q3LFR2 L0N5I8 Q9VUF8

Pfam

PF00067 p450 + More
PF03109 ABC1

Interpro

IPR036396   Cyt_P450_sf        + More
IPR001128   Cyt_P450
IPR017972   Cyt_P450_CS
IPR002401   Cyt_P450_E_grp-I
IPR000719   Prot_kinase_dom
IPR004147   UbiB_dom
IPR011009   Kinase-like_dom_sf

SUPFAM

SSF48264 SSF48264 + More
SSF56112 SSF56112

Gene 3D

1.10.630.10

ProteinModelPortal

Q3LFR2 Q2EMR3 L0N5I8 S6CBA3 A0A0P0I9N6 A0A2W1BUM3 + More
A0A2A4J8X4 A0A248QFG1 D2DEB0 A0A212EJE2 A0A1V0D9F0 A0A194Q2Z0 A0A0K8TT59 A0A1W4U9W6 A0A0R1DY93 A0A1W4ULA8 A0A0Q9XDI0 A0A0J9RVI8 B4HHR3 B4QK08 A0A0Q5U4F8 M9PI59 Q9VUF8 A0A2J7QGF4 B4PIE9 A0A2J7QGG0 B3M3F4 R9UA55 T1HSC5 B4KVM7 B4IWT4 A0A336MAY2 B4LEW8 A0A3B0JL24 A0A2S2Q0C8 Q2LZS4 A0A0R3P4A0 A0A0R3P476 A0A1Q1NKZ6 A0A0M4F155 B3ND40 A0A1B6EHD4 A0A1I8MBY6 K4JQD8 B4NM34 A0A1I9W2S4 A0A0K0LB70 E0VHL8 A0A2P8Y5W6 A0A1B6FWG4 M9SV74 U5KSD0 A0A0M3QWR1 M9SV71 A0A1I8Q469 A0A146KXT8 A0A0A9XMH1 A0A023I730 A0A1A9VHQ2 A0A1B0AFX2 A0A0A1WTZ6 A0A224XR57 A0A1A9XC80 A0A1B0AKY9 A0A1B6J7Y8 A0A1A9W8F7 A0A023F626 K9LCM6 A0A0K8VUZ4 A0A1B0G8W1 A0A034W759 A0A2H8TX85 J9K5U5 A0A0L0C4U9 A0A3G1ZJK6 A0A1B0DGD6 A0A1L8E416 E9JB01 A0A1B0CKE9 A0A0C9R3M8 E2AC01 A0A2S2QKE6 A0A182G832 A0A1S4J4E5 A0A0N0BJD8 A0A0C5BXD2 A0A067RDA3 J9KA32 Q563C4 A0A182MPH0 B0W3J4 A0A182VX95 A0A0T6AY57 K0GJA0 B4H842

PDB

3K9Y E-value=1.26855e-13, Score=185

KEGG

100127102 K10723 ecdysone 20-monooxygenase [EC:1.14.99.22] | (RefSeq) Cyp314a1; ecdysone 20-hydroxylase

GO

GO:0005506 GO:0020037 GO:0016021 GO:0004501 GO:0004497 GO:0016705 GO:0008258 GO:0007417 GO:0007295 GO:0007391 GO:0007494 GO:0045456 GO:0005739 GO:0035074 GO:0007298 GO:0031966 GO:0046872 GO:0005524 GO:0004672 GO:0005634 GO:0005665 GO:0043565 GO:0048384 GO:0055114

Subcellular location

Mitochondrion membrane

Length:

350

Number of predicted TMHs:

1

Exp number of AAs in TMHs:

27.10392

Exp number, first 60 AAs:

21.26261

Total prob of N-in:

0.24717

POSSIBLE N-term signal

sequence

outside

1 - 14

TMhelix

15 - 37

inside

38 - 350

Pi

251.589306

Theta

187.935644

Tajima's D

1.001886

CLR

0.02365

CSRT

0.661866906654667

Interpretation

Uncertain

Sequence

Summary

Ontologies

Topology

Population Genetic Test Statistics