Gene

KWMTBOMO03932  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA010147

Annotation

PREDICTED:_E3_ubiquitin-protein_ligase_KCMF1_[Bombyx_mori]

Location

Bomo_Chr7(-):6755178-6762564      View in EpiBrowser

Full name

E3 ubiquitin-protein ligase Kcmf1       + More
E3 ubiquitin-protein ligase KCMF1      

Alternative Name

RING-type E3 ubiquitin transferase KCMF1

Location in the cell

Nuclear   Reliability : 2.431

CDS

ATGAATCGCCATGAAGGAGTCAGCTGTGATTCTTGTTTGAAAAATAACTTTAGAGGACGACGATACAAATGTTTGATTTGCATCGATTACGACTTGTGCGCTACTTGCTACGAGTCCGGCGCTACCACGAATCAACACACTACCGAACACCCCATGCAGTGCATTCTTTCTAGAAGTGATTTTGATTTATATTATGGAGGAGAAGCATTAACATTGGAACAGTCACAGGCGTACACGTGTCCTTTTTGTAACCGCATGGGTTTTACCGACACTGGTCTCATGGAGCATGTAACGGCGGAACACGCTGACACAACCCTTGCCGTAGTTTGTCCGGTGTGTGCATCAATGCCAGGTGGTGAGCCTAATTTCGTCACGGATGACTTTGCTGGCCATCTTACACTCGAGCACAGGACCGGGTCTAGAGATCTCATCTCTTTTTTGGATGAACCCAGTGGTATCCGGCATGGCGGAGTACGACGCATGCCCCACTCCGGTCGCGGCGTGAGTCGCGCGCGCCGTACCAACATGCAGTTCAGCACCGGTGGGGGCGGGATCTCGTCGCTGTCGCCGTCGTCCCGGGACGCGGTGGTGGATCCGATCGCGGAGCTCCTCTCCCAGCTGTCGGGCGTGCGGCGCGGCGCGCAGCCCGGCACTCCCAGCCAGCTCCAGCAGCTACAGATGCAGCTGCAGCTTGAACGACACCAGGCAACTGTCAATATCATTTTATTTCTTCTACATCTCGCTCGTTATTACACGCTTTATATTAGCTTCACTTGTATGTATGTTTGTAACTGA

Protein

MNRHEGVSCDSCLKNNFRGRRYKCLICIDYDLCATCYESGATTNQHTTEHPMQCILSRSDFDLYYGGEALTLEQSQAYTCPFCNRMGFTDTGLMEHVTAEHADTTLAVVCPVCASMPGGEPNFVTDDFAGHLTLEHRTGSRDLISFLDEPSGIRHGGVRRMPHSGRGVSRARRTNMQFSTGGGGISSLSPSSRDAVVDPIAELLSQLSGVRRGAQPGTPSQLQQLQMQLQLERHQATVNIILFLLHLARYYTLYISFTCMYVCN

Description

Has intrinsic E3 ubiquitin ligase activity and promotes ubiquitination. Involved in the negative regulation of the Ras/MAPK signaling pathway in the wing by acting with the E2 enzyme Unc6 and the putative E3 ligases poe and Ufd4 to mediate the ubiquitination and proteasomal degradation of rl/MAPK.
Has intrinsic E3 ubiquitin ligase activity and promotes ubiquitination.

Catalytic Activity

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.

Subunit

Interacts with poe.

Similarity

Belongs to the KCMF1 family.

Keywords

Complete proteome   Metal-binding   Reference proteome   Transferase   Ubl conjugation pathway   Zinc   Zinc-finger   Coiled coil  

Feature

chain  E3 ubiquitin-protein ligase Kcmf1

Uniprot

S4PYE0   A0A2H1WI49   A0A194QZA1   A0A194PLB1   A0A212FAG4   A0A2W1BQN3   + More
A0A182V3J7   A0A1Q3FAU6   A0A336M811   A0A1Q3FAZ4   A0A1B6EP31   A0A182GR99   A0A1Q3FAW1   A0A1L8DSN1   A0A1S4EW06   A0A1B6HJ09   A0A1B0GKY9   A0A2J7QSF3   A0A2J7QSG6   A0A2M3Z175   A0A2M4BIQ1   A0A2M4A8H0   A0A2M4A987   A0A2M4BIU2   A0A1Q3FAY0   Q17PB0   A0A2C9GR31   A0A0K8TRY1   A0A1B6CD46   A0A1W4XJM1   V5G9B9   U4UWJ2   N6UDY5   A0A2J7QSG2   U5EYS5   A0A2J7QSF2   A0A1W4XKI6   D6W7D8   A0A0T6AZ25   B3M204   Q7QCK5   A0A067QVW4   A0A182NR88   A0A026WHY3   A0A0K8UHV5   A0A0K8W5W3   A0A1W4W289   A0A1A9UN96   W8BLD2   B5DWI5   Q95RX5   A0A0A1XNA6   A0A034VU14   B4KBU6   A0A0R1E689   B4NA68   B4PTZ1   A0A182FQ58   B4M457   A0A182XAH6   A0A1S3J089   A0A0C9RVL4   A0A1I8MLP9   A0A1I8MLP7   T1P8K4   T1PAN7   A0A1I8MLP8   A0A0R3NJL0   A0A158ND19   W8C1J3   A0A1I8NLF2   T1JLN5   A0A1S3D3T4   A0A1I8NLE5   K7IT80   A0A1I8NLE7   A0A2D0QBU4   A0A0F8AMG7   H3A7Y3   A0A2R5L5J1   A0A1S3J1I1   A0A293N600   Q7T321   V9KN73   A0A3B0JUD7   A0A1Z5LG59   A0A3B4BPX1   A0A2A3EJM6   V9IKS9   A0A088AGJ2   A0A1A7XFZ6   F6RF07   A0A1Y1MRI2   A0A337SPV8   A0A1W5ADY9   A0A3Q0D405   A0A3Q2GIU3   B1H326  

EC Number

2.3.2.27

Pubmed

23622113    26354079    22118469    28756777    26483478    17510324    + More
26369729    23537049    18362917    19820115    17994087    18057021    12364791    24845553    24508170    30249741    24495485    15632085    10731132    12537572    12537569    27552662    25830018    25348373    17550304    25315136    23185243    21347285    20075255    25835551    9215903    15520368    23594743    24402279    28528879    17495919    28004739    17975172    20431018   

EMBL

GAIX01003453    JAA89107.1    ODYU01008814    SOQ52738.1    KQ460930    KPJ10787.1    + More
KQ459600    KPI94221.1    AGBW02009472    OWR50735.1    KZ149930    PZC77409.1    GFDL01010349    JAV24696.1    UFQT01000586    SSX25511.1    GFDL01010363    JAV24682.1    GECZ01030082    JAS39687.1    JXUM01082063    JXUM01082064    KQ563287    KXJ74124.1    GFDL01010358    JAV24687.1    GFDF01004697    JAV09387.1    GECU01033151    JAS74555.1    AJWK01033962    AJWK01033963    AJWK01033964    AJWK01033965    NEVH01011876    PNF31519.1    PNF31515.1    GGFM01001522    MBW22273.1    GGFJ01003771    MBW52912.1    GGFK01003776    MBW37097.1    GGFK01004042    MBW37363.1    GGFJ01003762    MBW52903.1    GFDL01010311    JAV24734.1    CH477193    EAT48522.1    APCN01000261    GDAI01000504    JAI17099.1    GEDC01025944    JAS11354.1    GALX01001731    JAB66735.1    KB632399    ERL94640.1    APGK01039147    KB740967    ENN76872.1    PNF31516.1    GANO01000099    JAB59772.1    PNF31518.1    KQ971307    EFA11042.1    LJIG01022526    KRT80109.1    CH902617    EDV43328.2    KPU80205.1    KPU80206.1    AAAB01008859    EAA08156.5    KK853313    KDR08653.1    KK107213    QOIP01000007    EZA55281.1    RLU19999.1    GDHF01026168    JAI26146.1    GDHF01006069    JAI46245.1    GAMC01006953    JAB99602.1    CM000070    EDY68555.3    AE014297    AY061066    BT001502    BT001483    BT024222    AAF54323.3    AAL28614.1    AAN71238.1    AAN71257.1    ABC86284.1    GBXI01011579    GBXI01002224    JAD02713.1    JAD12068.1    GAKP01013638    GAKP01013637    JAC45315.1    CH933806    EDW15798.2    KRG01738.1    CM000160    KRK03226.1    KRK03227.1    CH964232    EDW80711.2    EDW96602.2    KRK03223.1    KRK03224.1    KRK03225.1    KRK03228.1    CH940652    EDW59418.2    GBYB01012995    JAG82762.1    KA644972    KA644974    AFP59601.1    KA644971    KA644973    AFP59602.1    KRT01216.1    KRT01217.1    KRT01218.1    KRT01219.1    ADTU01012183    GAMC01006954    GAMC01006952    JAB99601.1    JH430212    AAZX01003293    AAZX01005794    KQ041734    KKF23443.1    AFYH01168825    AFYH01168826    AFYH01168827    AFYH01168828    AFYH01168829    AFYH01168830    AFYH01168831    AFYH01168832    AFYH01168833    AFYH01168834    GGLE01000617    MBY04743.1    GFWV01022891    MAA47618.1    AY394941    BX294395    BC053288    JW867183    AFO99700.1    OUUW01000008    SPP84012.1    GFJQ02000976    JAW05994.1    KZ288235    PBC31409.1    JR049769    AEY61071.1    HADW01015577    SBP16977.1    GEZM01026236    JAV87125.1    AANG04002776    AAMC01029066    AAMC01029067    AAMC01029068    AAMC01029069    AAMC01029070    AAMC01029071    AAMC01029072    AAMC01029073    BC161222    AAI61222.1   

Proteomes

UP000053240    UP000053268    UP000007151    UP000075903    UP000069940    UP000249989    + More
UP000092461    UP000235965    UP000008820    UP000075840    UP000192223    UP000030742    UP000019118    UP000007266    UP000007801    UP000007062    UP000027135    UP000075884    UP000053097    UP000279307    UP000192221    UP000078200    UP000001819    UP000000803    UP000009192    UP000002282    UP000007798    UP000069272    UP000008792    UP000076407    UP000085678    UP000095301    UP000005205    UP000095300    UP000079169    UP000002358    UP000221080    UP000008672    UP000000437    UP000268350    UP000261440    UP000242457    UP000005203    UP000002280    UP000011712    UP000192224    UP000189706    UP000265020    UP000008143   

PRIDE

Q95RX5    A0A088AGJ2   

Pfam

PF05605   zf-Di19        + More
PF00569   ZZ
PF01762   Galactosyl_T

Interpro

IPR008598   Di19_Zn_binding_dom        + More
IPR039858   KCMF1       
IPR000433   Znf_ZZ       
IPR035994   Nucleoside_phosphorylase_sf       
IPR013087   Znf_C2H2_type       
IPR002659   Glyco_trans_31       

SUPFAM

SSF53167   SSF53167       

ProteinModelPortal

S4PYE0   A0A2H1WI49   A0A194QZA1   A0A194PLB1   A0A212FAG4   A0A2W1BQN3   + More
A0A182V3J7   A0A1Q3FAU6   A0A336M811   A0A1Q3FAZ4   A0A1B6EP31   A0A182GR99   A0A1Q3FAW1   A0A1L8DSN1   A0A1S4EW06   A0A1B6HJ09   A0A1B0GKY9   A0A2J7QSF3   A0A2J7QSG6   A0A2M3Z175   A0A2M4BIQ1   A0A2M4A8H0   A0A2M4A987   A0A2M4BIU2   A0A1Q3FAY0   Q17PB0   A0A2C9GR31   A0A0K8TRY1   A0A1B6CD46   A0A1W4XJM1   V5G9B9   U4UWJ2   N6UDY5   A0A2J7QSG2   U5EYS5   A0A2J7QSF2   A0A1W4XKI6   D6W7D8   A0A0T6AZ25   B3M204   Q7QCK5   A0A067QVW4   A0A182NR88   A0A026WHY3   A0A0K8UHV5   A0A0K8W5W3   A0A1W4W289   A0A1A9UN96   W8BLD2   B5DWI5   Q95RX5   A0A0A1XNA6   A0A034VU14   B4KBU6   A0A0R1E689   B4NA68   B4PTZ1   A0A182FQ58   B4M457   A0A182XAH6   A0A1S3J089   A0A0C9RVL4   A0A1I8MLP9   A0A1I8MLP7   T1P8K4   T1PAN7   A0A1I8MLP8   A0A0R3NJL0   A0A158ND19   W8C1J3   A0A1I8NLF2   T1JLN5   A0A1S3D3T4   A0A1I8NLE5   K7IT80   A0A1I8NLE7   A0A2D0QBU4   A0A0F8AMG7   H3A7Y3   A0A2R5L5J1   A0A1S3J1I1   A0A293N600   Q7T321   V9KN73   A0A3B0JUD7   A0A1Z5LG59   A0A3B4BPX1   A0A2A3EJM6   V9IKS9   A0A088AGJ2   A0A1A7XFZ6   F6RF07   A0A1Y1MRI2   A0A337SPV8   A0A1W5ADY9   A0A3Q0D405   A0A3Q2GIU3   B1H326  

PDB

4XIB     E-value=5.65705e-05,     Score=109

KEGG

101742658    K22376 E3 ubiquitin-protein ligase KCMF1 [EC:2.3.2.27] | (RefSeq) E3 ubiquitin-protein ligase KCMF1

GO

GO:0008270   GO:0016874   GO:0061630   GO:0009116   GO:0003676   GO:0016020   GO:0006486   GO:0008378   GO:0005829   GO:0046872   GO:0015074   GO:0006355   GO:0043565   GO:0016021   GO:0004672   GO:0048384  

PANTHER

PTHR12268:SF13  

Length:

264

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

16.39843

Exp number, first 60 AAs:

0.00228

Total prob of N-in:

0.38814

outside

1  -  264

 

 

Pi

270.674376

Theta

175.588306

Tajima's D

1.70977

CLR

0.185735

CSRT

0.831108444577771

Interpretation

Uncertain