Gene

KWMTBOMO03910

Pre Gene Modal

BGIBMGA010218

Annotation

PREDICTED:_D-tyrosyl-tRNA(Tyr)_deacylase_1_[Papilio_polytes]

Location

Bomo_Chr7(+):6296896-6300114      View in EpiBrowser

Full name

D-aminoacyl-tRNA deacylase       + More
D-aminoacyl-tRNA deacylase 1      

Alternative Name

DNA-unwinding element-binding protein B
Gly-tRNA(Ala) deacylase
Histidyl-tRNA synthase-related

Location in the cell

Cytoplasmic   Reliability : 1.496 Nuclear   Reliability : 1.85

CDS

ATGAAAGCACTAATACAACGAGTAATGAATGCCAAAGTTATAGTAAATGGTGAAGTCATTAGTAACATAGAACAAGGTCTATGCATACTTATCGGCATATCCAAATATGATACTGTCAAAGATATCGAATATATAGTGAGGAAGATTCTAAGTATCAAGTTATTTGATGACGAGTCCTCTAAGAGATGGAAGAAAACAGTAGTTGATAAACAATTTGAAATATTATGTGTCAGTCAATTCACATTATATAATACTTGGAAAGGTAACAAACCAGACTTCCACAATGCAATGCCTGGTGACCAATCTAAAGAATTTTACGAGAAATTTCTGCAGATGTTAAAAGACGCATATGTGTCTGATAAAATAAAAGATGGTTTATTTGGAGCCTACATGCAAGTTTGCATACAGAACGATGGACCTGTGACTTTTGAAATTGAATCCCTTGACAAGGACCCAAGCAAGAGTAATAAAAAAACAACAAGCTTAGAAGCAGGAGAAAGTATAGACTCTTAA

Protein

MKALIQRVMNAKVIVNGEVISNIEQGLCILIGISKYDTVKDIEYIVRKILSIKLFDDESSKRWKKTVVDKQFEILCVSQFTLYNTWKGNKPDFHNAMPGDQSKEFYEKFLQMLKDAYVSDKIKDGLFGAYMQVCIQNDGPVTFEIESLDKDPSKSNKKTTSLEAGESIDS

Description

An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality.
ATPase involved in DNA replication, may facilitate loading of CDC45 onto pre-replication complexes.
Possible ATPase (PubMed:15653697) involved in DNA replication, may facilitate loading of CDC45 onto pre-replication complexes (PubMed:20065034).

Catalytic Activity

a D-aminoacyl-tRNA + H2O = a D-alpha-amino acid + H(+) + tRNA
glycyl-tRNA(Ala) + H2O = glycine + H(+) + tRNA(Ala)

Subunit

Homodimer. Interacts with CDC45 and TOPBP1 (By similarity).
Homodimer (PubMed15653697). Interacts with CDC45 and TOPBP1 (PubMed:20065034).

Similarity

Belongs to the DTD family.

Keywords

Complete proteome   Cytoplasm   DNA replication   DNA-binding   Hydrolase   Metal-binding   Nucleus   Phosphoprotein   Reference proteome   RNA-binding   tRNA-binding   3D-structure   Alternative splicing   Direct protein sequencing  

Feature

chain  D-aminoacyl-tRNA deacylase 1
splice variant  In isoform 2.

Uniprot

A0A3S2M7P6   A0A2W1BRF8   A0A194QZD0   A0A2A4JN66   A0A194PLD5   A0A2H1WHF2   + More
S4P4E0   H9JL17   A0A3M6UHT7   A0A1E1WYV2   C3YA83   A0A2B4RE83   A0A369RXG0   B3RKW5   A0A023GFH5   A0A212F6U1   A0A1S3J8J8   A0A2T7NZ48   A0A131XH47   A0A131Z5R4   A0A1B0DDI3   W8BB04   Q2T9V8   L7M2W5   A0A2U3W7K9   A7RI71   A0A023GIL3   A0A2Y9DLI3   A0A2Y9R634   T1GKS1   A0A2Y9R6G8   A0A2K6DDC5   A0A2K5WFX0   F6SFP9   A0A2Y9HXR9   A0A2U3XHB2   A0A3Q7PFV1   A0A224YVD4   A0A250YCE3   G1T0D8   E1BRV8   A0A3L8SS01   A0A218V7D6   B5G2B1   U3JYZ5   A0A210PVY3   A0A1S3G2I0   G3QCX7   H2QK09   F1SBH1   A0A2I3HZ32   A0A2I3N5U2   A0A2K6QSJ2   Q8TEA8   G1LH06   A0A2J8VDF1   A0A337SIY5   A0A1S2ZYZ1   A0A2C9LR57   A0A2K5D659   F7H1X1   A0A2U4AQ65   A0A340XNW5   A0A2Y9PSW1   U6CSR6   A0A2Y9IWB8   M3XQ78   A0A0B8RUS3   A0A1V4KB59   A0A0Q3R9H2   A0A2I0LV95   A0A2K5N517   A0A3Q7WB05   A0A2Y9SSF0   A0A1D2MCP1   A0A2Y9HXG6   G5CBB5   B4NAX3   A0A2K5Q3N8   G3GVV9   A0A3Q0CVU2   F6QE61   A0A2K6H075   Q9DD18   A0A0A1XFX7   W5NGZ5   Q9DD18-2   W5NGZ7   A0A2R8Y6X2   H0WXF8   G1KG77   B0K014   H0UYI6   A0A098M0P8   A0A3Q2HYQ6   A0A2G8LR42   Q6DEC9   A0A1W7RI18   K1QSG6   A0A3Q7W175  

EC Number

3.1.1.-
3.1.1.96

Pubmed

28756777    26354079    23622113    19121390    30382153    28503490    + More
18563158    30042472    18719581    22118469    28049606    26830274    24495485    25576852    17615350    17431167    25319552    28797301    28087693    21993624    15592404    30282656    17018643    20360741    28812685    16136131    30723633    25362486    12392168    14702039    11780052    15489334    15653697    18088087    18669648    19413330    19690332    20065034    20068231    21269460    21406692    22814378    23186163    24275569    17264083    20010809    17975172    15562597    25243066    25476704    23371554    27289101    21993625    17994087    21804562    26319212    19892987    16141072    19468303    17242355    21183079    25830018    15057822    22673903    29023486    27762356    26358130    22992520   

EMBL

RSAL01000013    RVE53354.1    KZ149942    PZC76901.1    KQ460930    KPJ10812.1    + More
NWSH01000998    PCG73168.1    KQ459600    KPI94246.1    ODYU01008367    SOQ51894.1    GAIX01006014    JAA86546.1    BABH01011486    RCHS01001489    RMX53233.1    GFAC01006989    JAT92199.1    GG666494    EEN62631.1    LSMT01000759    PFX14585.1    NOWV01000174    RDD38503.1    DS985241    EDV29445.1    GBBM01002819    JAC32599.1    AGBW02009969    OWR49455.1    PZQS01000008    PVD26447.1    GEFH01003151    JAP65430.1    GEDV01002229    JAP86328.1    AJVK01031973    GAMC01012367    JAB94188.1    BC111244    GACK01006383    JAA58651.1    DS469511    EDO49100.1    GBBM01002818    JAC32600.1    CAQQ02064258    AQIA01008022    AQIA01008023    AQIA01008024    AQIA01008025    AQIA01008026    AQIA01008027    AQIA01008028    AQIA01008029    JSUE03004843    JSUE03004844    JSUE03004845    JSUE03004846    JU337107    JV048704    AFE80860.1    AFI38775.1    GFPF01007117    MAA18263.1    GFFW01003574    JAV41214.1    AAGW02052450    AAGW02052451    AAGW02052452    AAGW02052453    AAGW02052454    AAGW02052455    AAGW02052456    AAGW02052457    AADN05000005    QUSF01000008    RLW07069.1    MUZQ01000035    OWK61829.1    ABQF01122985    DQ216030    DQ216032    ACH45422.1    AGTO01022007    NEDP02005457    OWF40658.1    CABD030115800    CABD030115801    CABD030115802    CABD030115803    CABD030115804    CABD030115805    CABD030115806    CABD030115807    CABD030115808    CABD030115809    AACZ04064903    AACZ04064904    AACZ04064905    AACZ04064906    GABC01003204    GABF01006903    GABD01006265    GABE01008610    JAA08134.1    JAA15242.1    JAA26835.1    JAA36129.1    AEMK02000106    DQIR01268826    HDC24304.1    ADFV01042586    ADFV01042587    ADFV01042588    ADFV01042589    ADFV01042590    ADFV01042591    ADFV01042592    ADFV01042593    ADFV01042594    ADFV01042595    AHZZ02000689    AHZZ02000690    AHZZ02000691    AHZZ02000692    AHZZ02000693    AF332356    AK074304    AK291440    AL121900    AL121780    CH471133    BC000599    BC045167    BC100923    BC100924    BC100925    ACTA01152666    ACTA01160665    ACTA01168663    NDHI03003422    PNJ55545.1    AANG04003379    GAMT01007618    GAMS01008429    GAMS01008428    GAMR01006264    GAMQ01005768    GAMP01009581    JAB04243.1    JAB14707.1    JAB27668.1    JAB36083.1    JAB43174.1    HAAF01001695    CCP73521.1    AEYP01035809    AEYP01035810    AEYP01035811    AEYP01035812    AEYP01035813    AEYP01035814    AEYP01035815    GBSH01000834    JAG68191.1    LSYS01003963    OPJ81625.1    LMAW01002113    KQK81953.1    AKCR02000085    PKK21348.1    LJIJ01001850    ODM90682.1    JH204766    GEBF01001636    EHB18826.1    JAO01997.1    CH964232    EDW80937.1    JH000044    EGV95396.1    AK003594    AK010822    AK011917    AK017565    AK158898    AL808119    AL844516    BC026537    GBXI01004441    JAD09851.1    AHAT01001659    AAQR03145339    AAQR03145340    AAQR03145341    AAQR03145342    AAQR03145343    AAQR03145344    AAQR03145345    AAQR03145346    AAQR03145347    AAQR03145348    AABR07054070    AABR07054071    AABR07054072    BC159413    AAI59414.1    AAKN02012609    GBSI01000697    JAC95799.1    MRZV01000007    PIK62694.1    BC077198    CM004474    AAH77198.1    OCT79805.1    GDAY02000646    JAV50779.1    JH818024    EKC39842.1   

Proteomes

UP000283053    UP000053240    UP000218220    UP000053268    UP000005204    UP000275408    + More
UP000001554    UP000225706    UP000253843    UP000009022    UP000007151    UP000085678    UP000245119    UP000092462    UP000009136    UP000245340    UP000001593    UP000248480    UP000015102    UP000233120    UP000233100    UP000006718    UP000248481    UP000245341    UP000286641    UP000001811    UP000000539    UP000276834    UP000197619    UP000007754    UP000016665    UP000242188    UP000081671    UP000001519    UP000002277    UP000008227    UP000001073    UP000028761    UP000233200    UP000005640    UP000008912    UP000011712    UP000079721    UP000076420    UP000233020    UP000008225    UP000245320    UP000265300    UP000248483    UP000248482    UP000000715    UP000190648    UP000051836    UP000053872    UP000233060    UP000286642    UP000248484    UP000094527    UP000006813    UP000007798    UP000233040    UP000001075    UP000189706    UP000002281    UP000233160    UP000000589    UP000018468    UP000005225    UP000001646    UP000002494    UP000005447    UP000230750    UP000186698    UP000005408   

PRIDE

H9JL17    Q2T9V8    F1SBH1    Q8TEA8    G3GVV9    Q9DD18    + More

Pfam

PF02580   Tyr_Deacylase

Interpro

IPR003732   Daa-tRNA_deacyls_DTD        + More
IPR023509   DTD-like_sf       
IPR036600   PAH_sf       
IPR003822   PAH       

SUPFAM

SSF69500   SSF69500        + More
SSF47762   SSF47762       

Gene 3D

3.50.80.10    

CDD

cd00563  Dtyr_deacylase

ProteinModelPortal

A0A3S2M7P6   A0A2W1BRF8   A0A194QZD0   A0A2A4JN66   A0A194PLD5   A0A2H1WHF2   + More
S4P4E0   H9JL17   A0A3M6UHT7   A0A1E1WYV2   C3YA83   A0A2B4RE83   A0A369RXG0   B3RKW5   A0A023GFH5   A0A212F6U1   A0A1S3J8J8   A0A2T7NZ48   A0A131XH47   A0A131Z5R4   A0A1B0DDI3   W8BB04   Q2T9V8   L7M2W5   A0A2U3W7K9   A7RI71   A0A023GIL3   A0A2Y9DLI3   A0A2Y9R634   T1GKS1   A0A2Y9R6G8   A0A2K6DDC5   A0A2K5WFX0   F6SFP9   A0A2Y9HXR9   A0A2U3XHB2   A0A3Q7PFV1   A0A224YVD4   A0A250YCE3   G1T0D8   E1BRV8   A0A3L8SS01   A0A218V7D6   B5G2B1   U3JYZ5   A0A210PVY3   A0A1S3G2I0   G3QCX7   H2QK09   F1SBH1   A0A2I3HZ32   A0A2I3N5U2   A0A2K6QSJ2   Q8TEA8   G1LH06   A0A2J8VDF1   A0A337SIY5   A0A1S2ZYZ1   A0A2C9LR57   A0A2K5D659   F7H1X1   A0A2U4AQ65   A0A340XNW5   A0A2Y9PSW1   U6CSR6   A0A2Y9IWB8   M3XQ78   A0A0B8RUS3   A0A1V4KB59   A0A0Q3R9H2   A0A2I0LV95   A0A2K5N517   A0A3Q7WB05   A0A2Y9SSF0   A0A1D2MCP1   A0A2Y9HXG6   G5CBB5   B4NAX3   A0A2K5Q3N8   G3GVV9   A0A3Q0CVU2   F6QE61   A0A2K6H075   Q9DD18   A0A0A1XFX7   W5NGZ5   Q9DD18-2   W5NGZ7   A0A2R8Y6X2   H0WXF8   G1KG77   B0K014   H0UYI6   A0A098M0P8   A0A3Q2HYQ6   A0A2G8LR42   Q6DEC9   A0A1W7RI18   K1QSG6   A0A3Q7W175  

PDB

2OKV     E-value=1.05127e-47,     Score=474

KEGG

101740560    K07560 D-aminoacyl-tRNA deacylase [EC:3.1.1.96] | (RefSeq) D-tyrosyl-tRNA(Tyr) deacylase 1

GO

GO:0051500   GO:0000049   GO:0005737   GO:0051499   GO:0002161   GO:0006399   GO:0006260   GO:0003677   GO:0046872   GO:0005634   GO:0004812   GO:0005730   GO:0005829   GO:0106026   GO:0006355   GO:0016788   GO:0019478   GO:0006412   GO:0007219   GO:0007275   GO:0016021   GO:0004672   GO:0048384  

PANTHER

PTHR10472  

Subcellular location

Cytoplasm  
Nucleus   Associated with chromatin at some replication origins containing functional DNA-unwinding elements (By similarity).   With evidence from 3 publications.

Length:

170

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.08976

Exp number, first 60 AAs:

0.08827

Total prob of N-in:

0.25809

outside

1  -  170

 

 

Pi

319.862355

Theta

171.747773

Tajima's D

2.718713

CLR

0

CSRT

0.961601919904005

Interpretation

Uncertain